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The aerobic granular sludge (AGS) process is an effective wastewater treatment technology for organic matter and nutrient removal that has been introduced in the market rapidly. Until now, limited information is available on AGS regarding the removal of bacterial and viral pathogenic organisms present in sewage. This study focussed on determining the relation between reactor operational conditions (plug flow feeding, turbulent aeration and settling) and physical and biological mechanisms on removing two faecal surrogates, Escherichia coli and MS2 bacteriophages. Two AGS laboratory-scale systems were separately fed with influent spiked with 1.0 × 106 CFU/100 mL of E. coli and 1.3 × 108 PFU/100 mL of MS2 bacteriophages and followed during the different operational phases. The reactors contained only granular sludge and no flocculent sludge. Both systems showed reductions in the liquid phase of 0.3 Log10 during anaerobic feeding caused by a dilution factor and attachment of the organisms on the granules. Higher removal efficiencies were achieved during aeration, approximately 1 Log10 for E. coli and 0.6 Log10 for the MS2 bacteriophages caused mainly by predation. The 18S sequencing analysis revealed high operational taxonomic units (OTUs) of free-living protozoa genera Rhogostoma and Telotrochidium concerning the whole eukaryotic community. Attached ciliates propagated after the addition of the E. coli, an active contribution of the genera Epistylis, Vorticella, and Pseudovorticella was found when the reactor reached stability. In contrast, no significant growth of predators occurred when spiking the system with MS2 bacteriophages, indicating a low contribution of protozoa on the phage removal. Settling did not contribute to the removal of the studied bacterial and viral surrogates. M.L. Barrios-Hernández acknowledges the Technological Institute of Costa Rica for providing the fellowship (Grant Number 007-2014-M) to pursue her PhD programme (2016-2020) at IHE-Delft, the Netherlands. K. Mora-Cabrera acknowledges the Generalitat Valenciana (GRISOLIAP/2017/173) and the European Social Funds (BEFPI/2019/065) for their financial support.

This book describes pathogen removal processes in aerobic granular sludge (AGS) wastewater treatment systems. Faecal indicators (E. coli, Enterococci, coliforms and bacteriophages) were tracked in full-scale AGS facilities and compared to parallel activated sludge (CAS) systems. AGS showed similar removals as the more complex CAS configurations. Removal mechanisms investigated in laboratory-scale reactors showed that the AGS morphology contributes to the removal processes. By tracking E. coli and MS2, it was observed that organisms not attached to the granules are predated by protozoa during aeration. 18S RNA gene analyses confirmed the occurrence of bacterivorous organisms (e.g., Epistylis, Vorticella, Rhogostoma) in the system. Particulate material in the feeding stimulated their development, and a protozoa bloom arose when co-treating with (synthetic) faecal sludge (4 % v/v). An overview of the diverse eukaryotic community in laboratory reactors and real-life applications is also provided. The microbial diversity of the influent was different compared to AGS and CAS sludge samples. However, no clear differences were found between them on species level. This study contributes to a better understanding of the mechanisms behind pathogen removals in AGS systems.

The co-treatment of two synthetic faecal sludges (FS-1 and FS-2) with municipal synthetic wastewater (WW) was evaluated in an aerobic granular sludge (AGS) reactor. After characterisation, FS-1 showed the following concentrations, representative for medium-strength FS: 12,180 mg TSS L−1, 24,300 mg total COD L−1, 93.8 mg PO3-P L−1, and 325 mg NH4-N L−1. The NO3-N concentration was relatively high (300 mg L−1). For FS-2, the main difference with FS-1 was a lower nitrate concentration (18 mg L−1). The recipes were added consecutively, together with the WW, to an AGS reactor. In the case of FS-1, the system was fed with 7.2 kg total COD m−3d−1 and 0.5 kg Nitrogen m−3d−1. Undesired denitrification occurred during feeding and settling resulting in floating sludge and wash-out. In the case of FS-2, the system was fed with 8.0 kg total COD m−3d−1 and 0.3 kg Nitrogen m−3d−1. The lower NO3-N concentration in FS-2 resulted in less floating sludge, a more stabilised granular bed and better effluent concentrations. To enhance the hydrolysis of the slowly biodegradable particulates from the synthetic FS, an anaerobic stand-by period was added and the aeration period was increased. Overall, when compared to a control AGS reactor, a lower COD consumption (from 87 to 35 mg g−1 VSS h−1), P-uptake rates (from 6.0 to 2.0 mg P g VSS−1 h−1) and NH4-N removal (from 2.5 to 1.4 mg NH4-N g VSS−1 h−1) were registered after introducing the synthetic FS. Approximately 40% of the granular bed became flocculent at the end of the study, and a reduction of the granular size accompanied by higher solids accumulation in the reactor was observed. A considerable protozoa Vorticella spp. bloom attached to the granules and the accumulated particles occurred; potentially contributing to the removal of the suspended solids which were part of the FS recipe.

The aim of this study was to evaluate the effectiveness of the novel aerobic granular sludge (AGS) wastewater treatment technology in removing faecal indicator organisms (FIOs) compared to the conventional activated sludge (CAS) treatment system. The work was carried out at two full-scale wastewater treatment plants (WWTP) in the Netherlands, Vroomshoop and Garmerwolde. Both treatment plants have a CAS and AGS system operated in parallel. The parallel treatment lines are provided with the same influent wastewater. The concentrations of the measured FIOs in the influent of the two WWTPs were comparable with reported literature values as follows: F-specific RNA bacteriophages at 106 PFU/100 mL, and Escherichia coli (E. coli), Enterococci, and Thermotolerant coliforms (TtC) at 105 to 106 CFU/100 mL. Although both systems (CAS and AGS) are different in terms of design, operation, and microbial community, both systems showed similar FIOs removal efficiency. At the Vroomshoop WWTP, Log10 removals for F-specific RNA bacteriophages of 1.4 ± 0.5 and 1.3 ± 0.6 were obtained for the AGS and CAS systems, while at the Garmerwolde WWTP, Log10 removals for F-specific RNA bacteriophages of 1.9 ± 0.7 and 2.1 ± 0.7 were found for the AGS and CAS systems. Correspondingly, E. coli, Enterococci, and TtC Log10 removals of 1.7 ± 0.7 and 1.1 ± 0.7 were achieved for the AGS and CAS systems at Vroomshoop WWTP. For Garmerwolde WWTP Log10 removals of 2.3 ± 0.8 and 1.9 ± 0.7 for the AGS and CAS systems were found, respectively. The measured difference in removal rates between the plants was not significant. Physicochemical water quality parameters, such as the concentrations of organic matter, nutrients, and total suspended solids (TSS) were also determined. Overall, it was not possible to establish a direct correlation between the physicochemical parameters and the removal of FIOs for any of the treatment systems (CAS and AGS). Only the removal of TSS could be positively correlated to the E. coli removal for the AGS technology at the evaluated WWTPs., Graphical abstract Image 1, Highlights • No difference in removal efficiencies (α = 5%) of FIOs was found for the studied AGS and CAS systems. • 1.3 to 2.1 Log10 removals for F-specific RNA bacteriophages were found for the CAS and AGS systems. • 1.1 to 2.3 Log10 removals for E. coli, Enterococci, and TtC were obtained for the AGS and CAS systems. • The two stage CAS performed better than the carrousel CAS in removing F-specific RNA bacteriophages. • A positive correlation was found between the TSS and E. coli removal, and COD and TtC removal in the AGS systems.

Este artículo documenta la situación de la gestión integral de los residuos sólidos en la Municipalidad de Alvarado, mediante el análisis de los avances, características, fortalezas y debilidades que ha experimentado en los últimos 10 años. Los resultados de esta investigación demuestran que dicha municipalidad es un ejemplo de buenas prácticas de gestión integral de residuos sólidos en el ámbito nacional, a pesar que la Contraloría General de la República la clasifica como poco satisfactoria. Para esta investigación se recopiló y analizó la información bibliográfica disponible de la zona, se desarrollaron instrumentos de captura de información, se entrevistaron los principales actores sociales, se realizaron visitas de campo y se analizó toda esta información, de forma que se logró sistematizar y organizar en los siguientes tópicos: 1) Gestión administrativa de los residuos sólidos: porcentajes de cobertura de recolección, limpieza de parques y vías, relleno sanitario que se utiliza y estado actual del antiguo botadero municipal; 2) descripción y reseña del programa de gestión integral de residuos sólidos, descripción de la implementación de la ley de Gestión Integral de Residuos (GIR) y su relación con los indicadores utilizados por la Controlaría General de la República. This paper documents the situation of integral management of solid waste in the Alvarado Municipality. We analyzed the characteristics, strengths and weaknesses, that the municipality has experimented in the last 10 years, related with the solid waste. The results of this research, shows that Alvarado Municipality is an example of good management of solid waste at national level, despite that the “Contraloría General de la Republica” classifies them as unsatisfactory. We collected and analyzed the bibliographic information available, interviewed the principal social actors, developed instruments for collection of information, made field work, resumed and analyzed all that information, in order that we could systemize and analyze the next topics: 1) Management of the solid waste: covertures percent, characteristics of street and park cleaning, description of municipal landfill, 2) description of the integral management of solid waste, implementation of the GIR Law of the integral management of solid waste, and description of the Contraloría General de la República indicators for environmental efficiencies.

En este artículo se presentan los resultados obtenidos al desarrollar tres nuevas formulaciones a partir de materiales poliméricos generados en el desecho doméstico, con el fin de determinar el mejor material para fabricar poliducto de riego en Costa Rica, utilizando poliducto comercial como referencia. Se recuperó material polimérico postconsumo, específicamente polietilenos y polipropilenos, con la ayuda de actores de programas de recuperación de desechos del Tecnológico de Costa Rica. El material requirió de procesos como limpieza, clasificación, molienda y la extrusión de láminas. Las muestras se prepararon con diferentes proporciones de los polímeros utilizados. Un primer especimen fue de polietileno de baja densidad, otra mezcla fue de 75% de polietileno de baja densidad y 25% de polietileno de alta densidad, y una última mezcla de 75% de polietileno de alta densidad y 25% de polipropileno. A estas muestras se les determinaron propiedades mecánicas y térmicas. Como principal resultado se obtuvo que la formulación que mejoró las propiedades estudiadas del poliducto fue la mezcla de diferentes productos de Polietileno de Baja Densidad postconsumo, para un total de 10 repeticiones con 95% de confianza en la prueba de Kolmogorov-Smirnov, concluyendo que este producto supera las propiedades de resistencia en elongación y flexión y que necesita la optimización de las características para resistencia al impacto modo IZOD. Además, la prueba de calorimetría diferencial de barrido demostró la ausencia de agentes externos en la muestras y el análisis termogravimétrico reveló cambios de temperatura de descomposición semejantes al ducto y mayores a las otras mezclas. It aims to show the results to develop three new formulations of polymeric materials from household waste generated, in order to determine the best material to make irrigation pipeline in Costa Rica, using commercial pipeline reference. Polymeric material was recovered polyethylene and polypropylene post-consumer, with the help of ITCR waste recovery programs. The material required for processes such as cleaning, sorting, grinding and extrusion. The samples were prepared with different proportions of the polymers used. The first specimen was LDPE; another mix was 75 % of LDPE and 25 % HDPE and a final mixture of 75 % of HDPE and 25 % of PP. These samples were analyzed for mechanical and thermal properties. As a main result we found that the improved formulation of the pipeline properties studied was the mixture of different post-consumer LDPE products, for a total of ten repetitions with 95 % confidence in the Kolmogorov- Smirnov test, concluding that this product exceeds elongation resistance properties in bending and optimization needed characteristics for IZOD impact strength mode. Testing differential scanning calorimetry showed no external agents in samples, and thermogravimetric analysis showed the decomposition temperature change similar to the duct and higher than the other blends.