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In the academic year 2020-21 Middlesex University maths students accessed all learning sessions remotely. Each of these interactive sessions was live-streamed, recorded and uploaded to our Virtual Learning Environment, providing hundreds of hours of recorded, unedited maths lecture for students to review.This case study reports on a project (partially funded by an IMA Education Grant) in which we invited undergraduates to reflect on their remote learning experiences and curate these video lectures. Students were asked to identify the most engaging, useful and interesting segments, and categorise and explain their choices in free-text comments to help us develop our approach to remote lectures and video resources.A total of 33 video clips were identified by students across levels 4 to 6 on our specialist BSc Mathematics and BSc Mathematics with Computing programmes. In this paper we will discuss our findings, illustrate with examples clips, identify themes in the student choices, and conclude with tips to produce engaging video content.We will also discuss applications of video curation as a social pedagogic tool for the current Generation Z students. We will argue that sharing how students interact with digital learning resources can help address the significant digital divide in education.

The graduate skills expected of mathematics students from employers has changed dramatically over the past decade. Increasingly maths graduates are expected to have communication, creative, programming and teamwork skills in addition to the problem solving and critical thinking skills traditionally sought by employers from mathematics graduates. In this paper we will outline how the Middlesex University undergraduate mathematics programme was designed to enhance students’ communication, creativity and programming skills while maintaining the mathematical rigour that unpins all maths programmes.

ObjectiveEvaluate the effectiveness of systematically delivered evidence-based home safety promotion for improving child home safety practices.DesignControlled before-and-after study.SettingNine electoral wards in Nottingham, UK.Participants361 families with children aged 2–7 months at recruitment living in four intervention wards with high health, education and social need; and 401 in five matched control wards.InterventionEvidence-based home safety promotion delivered by health visiting teams, family mentors and children’s centres including 24 monthly safety messages; home safety activity sessions; quarterly ‘safety weeks’; home safety checklists.OutcomesPrimary: composite measure comprising having a working smoke alarm, storing poisons out of reach and having a stairgate. Secondary: other home safety practices; medically attended injuries. Parents completed questionnaires at 12 and 24 months after recruitment plus optional three monthly injury questionnaires.ResultsAt 24 months there was no significant difference between groups in the primary outcome (55.8% vs 48.8%; OR 1.58, 95% CI 0.98 to 2.55) or medically attended injury rates (incidence rate ratio 0.89, 95% CI 0.51 to 1.56), but intervention families were more likely to store poisons safely (OR 1.81, 95% CI 1.06 to 3.07), have a fire escape plan (OR 1.81, 95% CI 1.06 to 3.08), use a fireguard or have no fire (OR 3.17, 95% CI 1.63 to 6.16) and perform more safety practices (β 0.46, 95% CI 0.13 to 0.79).ConclusionsSystematic evidence-based home safety promotion in areas with substantial need increases adoption of some safety practices. Funders should consider commissioning evidence-based multicomponent child home safety interventions.Trial registration numberISRCTN31210493.

Objective: This cadaveric pilot study was to develop a weight bearing computed tomography (WBCT) three-dimensional (3D) distance mapping algorithm that would allow for detection of syndesmotic instability. Methods: Pilot study, two cadaveric specimens. Syndesmotic instability was induced by release of all syndesmotic ligaments through a conventional lateral ankle approach. WBCT imaging under simulated weight bearing was acquired before and after syndesmotic destabilization. Syndesmotic incisura and ankle gutter distances were assessed using a 3D distance mapping WBCT algorithm. Results: We found increases in the overall mean syndesmotic distances in the injured syndesmosis when compared to pre-injury state, and color coded distance maps allowed easy interpretation of the syndesmotic widening following ligament sectioning and destabilization of the syndesmotic joint. Conclusion: The WBCT 3D distance mapping algorithm has the potential to allow detection of mild syndesmotic instability with a relatively ease of interpretation by using color-coded distance maps. Level of Evidence V; Cadaveric Study.

Objective: The objective of the study was to assess the efficacy of the LapiCotton procedure on patients with hallux valgus (HV) combined with medial longitudinal arch collapse. Methods: Preoperative and postoperative weight-bearing computed tomography (WBCT) scans were obtained from patients with HV submitted to the LapiCotton procedure. Semi-automatic measurements were applied to 22 WBCT images across 11 patients enrolled in the study using a software package (Bonelogic, Disior™, Helsinki, Finland). Significance level was set at 0.05. Results: The hallux valgus angle (HVA) was significantly larger (p=0.026) in the preoperative group (Mdn = 27.52) than in the postoperative group (Mdn = 20). In addition, the Meary sagittal measurement was found to be significantly increased (p=0.033) in the preoperative group (Mdn = -14.28) when compared to the postoperative group (Mdn = -11.15). It was also observed that the intermetatarsal angle was significantly larger (p=0.003) in the preoperative group (Mdn = 15.68) compared to the postoperative group (Mdn = 11.26). Conclusion: The LapiCotton procedure effectively corrected radiographic parameters in patients with HV combined with the medial longitudinal arch collapse. Level of Evidence III; Therapeutic Studies; Comparative Retrospective Study.

Clinical diagnostic reports from 508 cases of canine demodicosis diagnosed either by histological or skin scraping analysis from a United Kingdom Accreditation Service (UKAS) accredited veterinary diagnostic laboratory servicing the United Kingdom (UK) and Ireland were evaluated. Of the 508 cases, 284 had skin swabs submitted for culture on the same day the skin biopsy and/or skin scraping were obtained. Dogs with juvenile-onset (JO) demodicosis represented 57.4% of these cases, whilst adult-onset (AO) cases comprised 42.6%. The data revealed that overgrowth of pathogenic bacteria was more common in AO demodicosis cases (75.2%) in comparison to the JO cases (57%). Adult-onset cases also had increased involvement of bacteria belonging to multiple genera and/or yeast (28.9%) in comparison to JO cases (18.4%). Pruritus was significantly associated with an overgrowth of Staphylococcus pseudintermedius (p 0.001). Resistance to one or more antimicrobial classes was noted in S. pseudintermedius isolates from 56.3% of JO cases with 10.3% of these cases being classified as Multi-Drug Resistant (MDR). Similarly, 51.9% of S. pseudintermedius isolates from the AO cases were noted to be resistant to one or more antimicrobial class with 8.6% of these cases being considered MDR. Cephalosporins were the most frequently administered antimicrobial class noted in submission histories, followed by the penicillin and fluoroquinolone classes. Whilst our findings reveal a high prevalence of concurrent overgrowth of pathogenic bacteria warranting therapeutic intervention in canine demodicosis, the presence of resistance within isolates highlights the need for prudent selection and targeted use of antimicrobial therapy that encompass the key principles of antimicrobial stewardship.

Canine demodicosis, due to an overpopulation of Demodex spp. mites, remains one of the most common dermatological diseases encountered in small animal practice. The aims of this study were to interrogate submitted histories and diagnostic report results from a large cohort of dogs (n = 508) diagnosed with demodicosis either through histological analysis or the finding of Demodex spp. mites on skin scrapings by a UKAS accredited commercial laboratory servicing the United Kingdom (UK) and Ireland in the years 2017 and 2018. The main findings revealed that short-coated breeds were more likely to develop juvenile-onset (JO) demodicosis, whereas medium- and long-coated breeds were more likely to develop adult-onset (AO) disease. Pododemodicosis was reported more commonly in adult, long-coated breeds. Skin scrapings were positive in only 83.3% of samples that had a corresponding positive biopsy result; this finding highlights the necessity to perform further diagnostic tests if demodicosis remains clinically suspected despite a negative skin scraping result. Concurrent underlying diseases, potentially associated with immunosuppression, were reported in 42/221 (19%) of dogs with AO demodicosis. Serum allergy and Sarcoptes ELISA assays were positive in individual animals in both the JO and AO groups; the clinical significance of these latter findings requires careful interpretation in dogs with confirmed demodicosis.

This paper presents a summary of work undertaken by the authors and their teams on a series of Qe'an (plural of Qa’), in the Badia of eastern Jordan. These basins are foci for settlement in the region, with the sites described here (Shubayqa, Wisad and the Qa’ Qattafi) edged by archaeological sites dating from the late Epipalaeolithic (ca. 14,500 - 11,600 cal BP) and the Neolithic (ca. 11,700 - 6100 cal BP), and in areas still used by people today as seasonal wetlands for watering animals and growing cereal. We assess here the potential for the Qe'an sediments to provide what would be rare continuous palaeoenvironmental records for this part of SW Asia.\ud \ud The paper presents the first dates from the Qe'an of this region and the outline sedimentology. Much of the fill is of Holocene age, which leads to discussion of climate and landscape change over the last 15,000 years, particularly due to the close geographical relationship between these basins and archaeology. Our optically stimulated luminescence and radiocarbon dating of the basin fill suggests that there was significantly more space in the landscape for water storage in the early Holocene, which may have therefore provided this resource for people and their livestock or game for a longer duration each year than that seen today. Linked to this are hypotheses of a more vegetated landscape during this time period. Given the environmentally marginal nature of our study area subtle changes in landscape and/or climate, and human exploitation of these resources, could have led to significant, and likely detrimental for its inhabitants, environmental impacts for the region, such as desertification. Our data are suggestive of desertification occurring, and sets up a clear hypothesis for testing by future work in the region.

During the COVID-19 pandemic, wastewater surveillance was leveraged as a powerful tool for monitoring community-scale health. Further, the well-known persistence of some pharmaceuticals through wastewater treatment plants spurred concerns that increased usage of pharmaceuticals during the pandemic would increase the concentrations in wastewater treatment plant effluent. We collected weekly influent and effluent samples from May 2020 through May 2021 from two wastewater treatment plants in central Pennsylvania, the Penn State Water Reclamation Facility and the University Area Joint Authority, that provide effluent for beneficial reuse, including for irrigation. Samples were analyzed for severe acute respiratory syndrome coronavirus 2 (influent only), two over-the-counter medicines (acetaminophen and naproxen), five antibiotics (ampicillin, doxycycline, ofloxacin, sulfamethoxazole, and trimethoprim), two therapeutic agents (remdesivir and dexamethasone), and hydroxychloroquine. Although there were no correlations between pharmaceutical and virus concentration, remdesivir detection occurred when the number of hospitalized patients with COVID-19 increased, and dexamethasone detection co-occurred with the presence of patients with COVID-19 on ventilators. Additionally, Penn State decision-making regarding instruction modes explained the temporal variation of influent pharmaceutical concentrations, with detection occurring primarily when students were on campus. Risk quotients calculated for pharmaceuticals with known effective and lethal concentrations at which 50% of a population is affected for fish, daphnia, and algae were generally low in the effluent; however, some acute risks from sulfamethoxazole were high when students returned to campus. Remdesivir and dexamethasone persisted through the wastewater treatment plants, thereby introducing novel pharmaceuticals directly to soils and surface water. These results highlight connections between human health and water quality and further demonstrate the broad utility of wastewater surveillance.

The action to reduce anthropogenic greenhouse gas emissions is severely constrained by the difficulty of locating sources and quantifying their emission rates. Methane emissions by the energy sector are of particular concern. We report results achieved with a new area monitoring approach using laser dispersion spectroscopy to measure path-averaged concentrations along multiple beams. The method is generally applicable to greenhouse gases, but this work is focused on methane. Nineteen calibrated methane releases in four distinct configurations, including three separate blind trials, were made within a flat test area of 175 m by 175 m. Using a Gaussian plume gas dispersion model, driven by wind velocity data, we calculate the data anticipated for hundreds of automatically proposed candidate source configurations. The Markov-chain Monte Carlo analysis finds source locations and emission rates whose calculated path-averaged concentrations are consistent with those measured and associated uncertainties. This approach found the correct number of sources and located them to be within9 m in more than 75% of the cases. The relative accuracy of the mass emission rate results was highly correlated to the localization accuracy and better than 30% in 70% of the cases. The discrepancies for mass emission rates were2 kg/h for 95% of the cases.

A 70-year-old retired male plumber was admitted with reduced consciousness following a recent onset of confusion and headache. He developed encephalopathy, seizures and myoclonus. He died despite treatment 10 weeks later. This article reports the clinicopathological conference presented at the Edinburgh Clinical Neurology Course 2021.

It is known that the water entry of a body with a recessed, cupped nose can suppress the splash and air cavity typically observed for solid body entry (Mathai, Govardhan & Arakeri, Appl. Phys. Lett., vol. 106, 2015, 064101). However, the interplay between the captive gas in the cup, the cavity and the splash is quite subtle and has not been thoroughly explored. Here we study the cavity and splash dynamics associated with the vertical water entry of cups and find a variety of regimes over a range of Weber numbers ( $We_D$ ) and dimensionless cup depths. Our parameter space spans a transition between slow-developing cavities with long closure times (low $We_D$ ) to fast-sealing cavities (high $We_D$ ). An important dynamic event is the evacuation of trapped gas from within the cup, which drives the ensuing cavity and splash behaviour. Through modelling, we predict the conditions for which the evacuating gas inflates a cavity that opens to the atmosphere versus inflating a submerged cavity that suppresses air entrainment from above the surface. We also compare our cup water entry findings to the impact phenomena observed for flat disks, which entrap gas on the front surface similar to cups. In doing so, we reveal the sensitivity of disk splash and cavity behaviour to impact angle, and show that disks share a common regime with cups, in which a thin splash quickly seals on the body. We deduce the mechanisms by which increasing cup depth delays the cavity seal time in this regime. These findings reveal that cups may in fact promote or suppress cavity growth, depending on the cup depth and impact conditions.

The climate of the past two thousand years (2k) provides context for current and future changes, and as such is vital for developing our understanding of the modern climate system. Building on previous phases of the PAGES 2k network, Phase 4 of the PAGES 2k Network paves the way for a new level of understanding of the global water cycle, including enhanced science-policy integration. Previous PAGES 2k network phases emphasised temperature reconstructions, fundamentally improving our understanding of global climate changes over the Common Era. These reconstructions received widespread recognition and were featured in the Summary for Policymakers of the IPCC’s Sixth Assessment Report. Integration of this data with state-of-the-art Earth systems models, proxy system models and data assimilation yielded a more comprehensive understanding of the associated physical drivers and climate dynamics. Phase 4 challenges our community to turn its focus towards hydroclimate. Our aim is to reconstruct hydroclimatic variability over the Common Era, from local to global spatial scales, at sub-annual to multi-centennial time scales, developing a process-level understanding of past hydroclimate events and variability. Our multi-faceted approach includes (1) developing new hydroclimate syntheses that are well-suited for data-model comparisons, (2) improving the interoperability and scope of existing data and model products, and (3) facilitating the translation of our science into evidence-based policy outcomes. In this presentation, we report on our activities and progress to date, particularly highlighting the early stages of our data synthesis efforts.

Consumption of contaminated produce remains a leading cause of foodborne illness. Increasingly, growers are altering agricultural practices and farm environments to manage food-safety hazards, but these changes often result in substantial economic, social, and environmental costs. Here, we present a comprehensive evidence synthesis evaluating the efficacy of soil, non-crop vegetation, animal, landscape, and irrigation water management strategies aimed at reducing produce-safety risk in North America. We systematically summarized findings from 78 peer-reviewed papers on the effect of 21 management practices on the prevalence, abundance, or survival of four foodborne pathogens (i.e., E. coli, Salmonella spp., Listeria spp., and Campylobacter spp.), resulting in 113 summaries. We then organized a 30-member expert panel, who used these summaries to evaluate the impact of each practice on food-safety outcomes. While more than half of the practices were too understudied to confidently evaluate their impact on food safety, the panel did identify several practices that were associated with reduced preharvest food-safety risks, including not using raw manure, separating crop and livestock production, and choosing low-risk irrigation sources. The panel also identified practices that appear ineffective at reducing food-safety risks, such as the removal of non-crop vegetation. Overall, these findings provide insights into the food-safety impacts of agricultural and land management practices that growers, auditors, and extension personnel can use to co-manage produce preharvest environments for food safety and other aims.

The distribution and transmission of Yersinia pestis , the bacterial agent of plague, responds dynamically to climate, both within wildlife reservoirs and human populations. The exact mechanisms mediating plague's response to climate are still poorly understood, particularly across large environmentally heterogeneous regions encompassing several reservoir species. A heterogeneous response to precipitation was observed in plague intensity across northern and southern China during the Third Pandemic. This has been attributed to the response of reservoir species in each region. We use environmental niche modelling and hindcasting methods to test the response of a broad range of reservoir species to precipitation. We find little support for the hypothesis that the response of reservoir species to precipitation mediated the impact of precipitation on plague intensity. We instead observed that precipitation variables were of limited importance in defining species niches and rarely showed the expected response to precipitation across northern and southern China. These findings do not suggest that precipitation–reservoir species dynamics never influence plague intensity but that instead, the response of reservoir species to precipitation across a single biome cannot be assumed and that limited numbers of reservoir species may have a disproportional impact upon plague intensity.

BackgroundPick’s disease (PiD) is a rare and predominantly sporadic form of frontotemporal dementia that is classified as a primary tauopathy. PiD is pathologically defined by argyrophilic inclusion Pick bodies and ballooned neurons in the frontal and temporal brain lobes. PiD is characterised by the presence of Pick bodies which are formed from aggregated, hyperphosphorylated, 3-repeat tau proteins, encoded by theMAPTgene. TheMAPTH2 haplotype has consistently been associated with a decreased disease risk of the 4-repeat tauopathies of progressive supranuclear palsy and corticobasal degeneration, however its role in susceptibility to PiD is unclear. The primary aim of this study was to evaluate the association betweenMAPTH2 and risk of PiD.MethodsWe established the Pick’s disease International Consortium (PIC) and collected 338 (60.7% male) pathologically confirmed PiD brains from 39 sites worldwide. 1,312 neurologically healthy clinical controls were recruited from Mayo Clinic Jacksonville, FL (N=881) or Rochester, MN (N=431). For the primary analysis, subjects were directly genotyped forMAPTH1-H2 haplotype-defining variant rs8070723. In secondary analysis, we genotyped and constructed the six-variantMAPTH1 subhaplotypes (rs1467967, rs242557, rs3785883, rs2471738, rs8070723, and rs7521).FindingsOur primary analysis found that theMAPTH2 haplotype was associated with increased risk of PiD (OR: 1.35, 95% CI: 1.12-1.64 P=0.002). In secondary analysis involving H1 subhaplotypes, a protective association with PiD was observed for the H1f haplotype (0.0% vs. 1.2%, P=0.049), with a similar trend noted for H1b (OR: 0.76, 95% CI: 0.58-1.00, P=0.051). The 4-repeat tauopathy risk haplotypeMAPTH1c was not associated with PiD susceptibility (OR: 0.93, 95% CI: 0.70-1.25, P=0.65).InterpretationThe PIC represents the first opportunity to perform relatively large-scale studies to enhance our understanding of the pathobiology of PiD. This study demonstrates that in contrast to its protective role in 4R tauopathies, theMAPTH2 haplotype is associated with an increased risk of PiD. This finding is critical in directing isoform-related therapeutics for tauopathies.FundingSee funding section

This paper analyses a live project collaboration between the Birmingham School of Architecture & Design and CoLab Dudley, a social innovation lab based on Dudley High Street. The project developed students’ sustainability competencies while contributing to social, environmental, and economic progression and regeneration of local communities, and explored regenerative futures for Dudley High Street 2030 through engaging students, academics, collaborators, and a wider network of local people in a two-way collaborative learning process.Using Tilbury and Mulà’s five principles of Education for Sustainable Development as a model, the collaboration was analysed to tease out how the work might impact the education of future practitioners. The research identifies a positive impact of real-life collaboration for students, academics, and collaborators in nurturing the conditions for radicality and reveals the conditions necessary for successful partnerships to develop. In going beyond technological solutions, the research reveals the potential of engaging students with real-world communities, participation, and future thinking to create radical practitioners ready to rise to the sustainability challenge

Investigative interviews (e.g., interrogations) are a critical component of criminal, military, and civil investigations. However, how levels of alertness (vs. sleepiness) of the interviewer impact outcomes of actual interviews is unknown. To this end, the current study tracked daily fluctuations in alertness among professional criminal investigators to predict their daily experiences with actual field interviews. Fifty law-enforcement investigators wore a sleep-activity tracker for two weeks while keeping a daily-diary of investigative interviews conducted in the field. For each interview, the investigators indicated how well they established rapport with the subject, how much resistance they encountered, how well they maintained their own focus and composure, and the overall utility of intelligence obtained. Daily alertness was biomathematically modeled from actigraphic sleep duration and continuity estimates and used to predict interview characteristics. Investigators consistently reported more difficulties maintaining their focus and composure as well as encountering more subject resistance during interviews on days with lower alertness. Better interview outcomes were also reported on days with subjectively better sleep, while findings were generally robust to inclusion of covariates. The findings implicate adequate sleep as a modifiable fitness factor for collectors of human intelligence.

Objective: To assess the agreement between semiautomatic 3D measurements and manual measurements derived from WBCT images in patients with end-stage ankle osteoarthritis (AO) who underwent total ankle replacement (TAR). Methods: In this retrospective, IRB-approved study (ID #201904825), we evaluated patients who underwent TAR via the lateral trans-fibular approach for end-stage ankle OA. The study included 14 ankles from 14 patients. Raw multiplanar data were analyzed using CubeVue® software. Lateral talar station (LTS) was obtained in the sagittal plane, while hindfoot moment arm (HMA) and talar tilt angle (TTA) were calculated in the coronal view. Semiautomatic 3D measurements were performed using Disior® software. Intra-rater reliabilities were analyzed using the intraclass correlation coefficient (ICC). Agreement between methods was tested with Bland-Altman plots. Each measurement was assessed using the Wilcoxon signed-rank test. Alpha risk was set to 5% (α=0.05). P-values of ≤0.05 were considered significant. Results: ICC-measured reliabilities ranged from moderate to almost perfect for manual and semiautomatic WBCT measurements in the preoperative and postoperative groups for HMA and LTS. There was high correlation between parameters calculated from manual and semiautomatic measurements, and strong agreement between the readers and software in both groups. Conclusions: Manual (M) and semiautomatic (SA) 3D measurements expressed excellent agreement for pre- and postoperative groups, indicating a high correlation between the parameters calculated and strong agreement between the readers and the software in both groups. Level of Evidence III; Therapeutic Studies; Comparative Retrospective Study.

This case study details several concrete approaches to integrating the use of student-loaned iPads in the teaching of mathematics in Higher Education. Although there is a scarcity of rigorous studies into the efficacy of tablet devices for improved educational outcomes, previous case studies have argued that tablet devices, if used, should be integrated into the whole learning experience. The mathematics teaching team at Middlesex University have developed an inclusive digital pedagogy over the last five years that enabled us to effectively respond to the remote teaching imposed by the COVID-19 pandemic by loaning iPads to all students on specialist mathematics programmes. As we begin the return to campus, we continue to integrate these devices into our teaching to address the observed “digital divide” in Generation-Z students which is characterised not by access to smart devices but by the digital skills to use them as effective learning tools. This is particularly relevant at Middlesex University which is disproportionately affected by digital poverty amongst its student population. We discuss the use of virtual whiteboard apps, the necessity of handwritten mathematics, the rich integration of multimedia content, persistent collaborative “problem solving spaces”, and how a common hardware platform allows for varied and equitable inclusive assessment. We also report the results of students’ surveys of iPad use during the remote-only 2020-21 academic year.

One approach to control dengue virus transmission is the symbiont Wolbachia, which limits viral infection in mosquitoes. Despite plans for its widespread use in Aedes aegypti, Wolbachia's mode of action remains poorly understood. Many studies suggest that the mechanism is likely multifaceted, involving aspects of immunity, cellular stress and nutritional competition. A previous study from our group used artificial selection to identify a new mosquito candidate gene related to viral blocking; alpha-mannosidase-2a (alpha-Mann-2a) with a predicted role in protein glycosylation. Protein glycosylation pathways tend to be involved in complex host-viral interactions; however, the function of alpha-mannosidases has not been described in mosquito-virus interactions. We examined alpha-Mann-2a expression in response to virus and Wolbachia infections and whether reduced gene expression, caused by RNA interference, affected viral loads. We show that dengue virus (DENV) infection affects the expression of alpha-Mann-2a in a tissue- and time-dependent manner, whereas Wolbachia infection had no effect. In the midgut, DENV prevalence increased following knockdown of alpha-Mann-2a expression in Wolbachia-free mosquitoes, suggesting that alpha-Mann-2a interferes with infection. Expression knockdown had the same effect on the togavirus chikungunya virus, indicating that alpha-Mann-2a may have broad antivirus effects in the midgut. Interestingly, we were unable to knockdown the expression in Wolbachia-infected mosquitoes. We also provide evidence that alpha-Mann-2a may affect the transcriptional level of another gene predicted to be involved in viral blocking and cell adhesion; cadherin87a. These data support the hypothesis that glycosylation and adhesion pathways may broadly be involved in viral infection in Ae. aegypti.

Introduction: Prevalence rates of cardiometabolic disease are rising, placing a significant burden on healthcare sectors, with cardiovascular disease (CVD) and type 2 diabetes contributing the most burden and being overweight the most contributing risk factor. With an ageing population, unfavourable risk factor trends and the challenges associated with CVD, we aimed to analyse a group of middle-class Australian adults for patterns in body composition, fitness and function. Methods: Data collected from adults attending a health clinic in eastern Sydney were analysed. Data were self-reported to or measured by an allied health clinician during initial consultations. Conditions reported included those contributing to the Australian disease burden in adults 45 years and over in 2018. Cardiometabolic multimorbidity was defined as the presence of two or more conditions simultaneously. Simple linear and stepwise regressions were used to assess potential relationships. Results: Data from 533 people (mean age 66.6±12.1 yrs; 67% female) were analysed. In total, 418 had a biological cardiometabolic risk factor or diagnosed CVD, of which 85.2% satisfied the criteria for cardiometabolic multimorbidity (73% female; 62% male), with most reporting 2-3 conditions. The most reported was coronary heart disease (64.4%), followed by hypertension (34.6%), type 2 diabetes (12.9%), cerebrovascular (5.1%), chronic kidney (1%) and peripheral artery (1%) disease. There was no relationship between the number of conditions and key outcomes, except for balance (r=0.12; p=0.03). There was a significant relationship between age and greater systolic blood pressure (SBP) (r=0.26; p Conclusion: Three in 4 Australian adults in this cohort had cardiometabolic multimorbidity, with a greater prevalence in females. Cardiometabolic multimorbidity was unrelated to key body composition parameters or fitness test performance. However, those that were older had a higher waist measurement and poorer lower body strength and performance in the 6-minute walk. Those with higher SBP also had a higher waist and lower body strength. With these fitness measures strongly related to cardiovascular mortality, more older adults need to participate in evidence-based healthy lifestyle education programs to help improve overall body composition, fitness and function, thereby reducing CVD.

Quantification of land surface-atmosphere fluxes of carbon dioxide (CO2) fluxes and their trends and uncertainties is essential for monitoring progress of the EU27+UK bloc as it strives to meet ambitious targets determined by both international agreements and internal regulation. This study provides a consolidated synthesis of fossil sources (CO2 fossil) and natural sources and sinks over land (CO2 land) using bottom-up (BU) and top-down (TD) approaches for the European Union and United Kingdom (EU27+UK), updating earlier syntheses (Petrescu et al., 2020, 2021b). Given the wide scope of the work and the variety of approaches involved, this study aims to answer essential questions identified in the previous syntheses and understand the differences between datasets, particularly for poorly characterized fluxes from managed ecosystems. The work integrates updated emission inventory data, process-based model results, data-driven sectoral model results, and inverse modeling estimates, extending the previous period 1990–2018 to the year 2020 to the extent possible. BU and TD products are compared with European National Greenhouse Gas Inventories (NGHGIs) reported by Parties including the year 2019 under the United Nations Framework Convention on Climate Change (UNFCCC). The uncertainties of the EU27+UK NGHGI were evaluated using the standard deviation reported by the EU Member States following the guidelines of the Intergovernmental Panel on Climate Change (IPCC) and harmonized by gap-filling procedures. Variation in estimates produced with other methods, such as atmospheric inversion models (TD) or spatially disaggregated inventory datasets (BU), originate from within-model uncertainty related to parameterization as well as structural differences between models. By comparing NGHGIs with other approaches, key sources of differences between estimates arise primarily in activities. System boundaries and emission categories create differences in CO2 fossil datasets, while different land use definitions for reporting emissions from Land Use, Land Use Change and Forestry (LULUCF) activities result in differences for CO2 land. The latter has important consequences for atmospheric inversions, leading to inversions reporting stronger sinks in vegetation and soils than are reported by the NGHGI. For CO2 fossil emissions, after harmonizing estimates based on common activities and selecting the most recent year available for all datasets, the UNFCCC NGHGI for the EU27+UK accounts for 3392 ± 49 Tg CO2 yr-1 (926 ± 13 Tg C yr-1), while eight other BU sources report a mean value of 3340 [3238,3401] [25th,75th percentile] Tg CO2 yr-1 (948 [937,961] Tg C yr-1). The sole top-down inversion of fossil emissions currently available accounts for 3800 Tg CO2 yr-1 (1038 Tg C yr-1), a value close to that of the NGHGI, but for which uncertainty estimates are not yet available. For the net CO2 land fluxes, during the most recent five-year period including the NGHGI estimates, the NGHGI accounted for -91 ± 32 Tg C yr-1 while six other BU approaches reported a mean sink of -62 [-117,-49] Tg C yr-1 and a 15-member ensemble of dynamic global vegetation models (DGVMs) reported -69 [-152,-5] Tg C yr-1. The five-year mean of three TD regional ensembles combined with one non-ensemble inversion of -73 Tg C yr-1 has a slightly smaller spread (0th–100th percentile of [-135,45] Tg C yr-1), and was calculated after removing land-atmosphere CO2 fluxes caused by lateral transport of carbon (crops, wood trade and inland waters) resulting in increased agreement with the the NGHGI and bottom-up approaches. Results at the sub-sector level (Forestland, Cropland, Grassland) show generally good agreement between the NGHGI and sub-sector-specific models, but results for a DGVM are mixed. Overall, for both CO2 fossil and net CO2 land fluxes, we find current independent approaches are consistent with the NGHGI at the scale of the EU27+UK. We conclude that CO2 emissions from fossil sources have decreased over the past 30 years in the EU27+UK, while large uncertainties on net uptake of CO2 by the land surface prevent trend identification. In addition, a gap on the order of 1000 Tg C yr-1 between CO2 fossil emissions and net CO2 uptake by the land exists regardless of the type of approach (NGHGI, TD, BU), falling well outside all available estimates of uncertainties. However, uncertainties in top-down approaches to estimate CO2 fossil emissions remain uncharacterized and are likely substantial. The data used to plot the figures are available at https://doi.org/10.5281/zenodo.7365863.

Thousands of babies are given toys for their zeroth birthday … But what happens if that baby is admitted to neonatal intensive care? In a global first, we describe the population of toys found in incubators on neonatal intensive care unit.

Domestic and family violence (DFV) is a pervasive social problem that social workers often encounter in practice. Responses to DFV require specialist and non-specialist services. Research suggests that first contact social workers can experience a lack of confidence, both in engaging with men who use violence and working with women and children who disclose experiencing violence. This article reports on the findings of an Australian survey (N = 100) that sought to identify the knowledge and practice skills that social workers draw on when they are the first responders to DFV. The survey invited qualified social worker participants from all sectors across Australia through the national online bulletins of the Australian Association of Social Workers (AASW) and Child Family Community Australia (CFCA). Whilst 28% of the respondents demonstrated working in specialist DFV and child protection sectors, 72% worked in other sectors. The research found that most participants recognised signs of DFV, but responses were mainly focused on referring women victims to practical or accommodation support, and children to psychological support. Responding to perpetrators was rarely mentioned. Equipping non-specialist DFV practitioners with knowledge and skills to respond safely can create an opportunity for engagement, earlier intervention, and increased interagency collaboration with specialist services. Refereed/Peer-reviewed

A working knowledge of statistical tests, their application, and interpretation is vital to evidence-based practice. A purposive sample of Doctor of Nursing Practice (DNP) projects listed in ProQuest (N = 667) were assessed for general use of statistics and data on the most frequently used statistical tests were compiled. Results enumerated the top 10 tests used with the top 3 methods reliant on basic and descriptive statistics. Application to practice and contributions to the evidence-base from DNP Projects are discussed. Potential statistical and methods issues are presented, along with some possible remedies. To improve project outcomes and professional practice, scoping of DNP statistical education is needed.

Concern that children are not engaging in enough physical activity (PA) to bring about health benefits is a crisis globally. This paper aims to examine primary school-based PA initiatives from with...

The synthesis and characterization of two homoleptic Zn(II)- and Mg(II)-complexes based on a simple tridentate {NNO} ligand are reported. The production of biocompatible atactic poly(lactic acid) (PLA) under industrially relevant melt conditions is demonstrated, noting high activity for Zn(1) 2at room temperature in CH 2Cl 2(TOF = 184 h -1). Mg(1) 2and Zn(1) 2were shown to facilitate rapid PLA methanolysis into methyl lactate (Me-LA) under mild conditions, achieving up to 85% Me-LA yield within 30 min at 50 °C in THF. Further kinetic analysis found Mg(1) 2and Zn(1) 2to exhibitk appvalues of 0.23 ± 0.0076 and 0.15 ± 0.0029 min -1, respectively {8 wt % cat. loading}, among the highest reported thus far. Zn(1) 2retained excellent activity for both poly(ethylene terephthalate) (PET) and poly(ε-caprolactone) (PCL) degradations, demonstrating catalyst versatility. Various upcycling strategies (e.g., methanolysis, glycolysis, and aminolysis) were employed to achieve a broad substrate scope, which included bis(2-hydroxyethyl) terephthalate (BHET), high value terephthalamides, and methyl 6-hydroxyhexanoate. Optimal glycolysis conditions using Zn(1) 2enabled 64% BHET yield within 1 h at 180 °C, a rare example of PET glycolysis mediated by a discrete homogeneous metal-based catalyst. The application of such catalysts for PET aminolysis and PCL methanolysis has been reported for the first time.

COVID-19 vaccination is being rapidly rolled out in the US and many other countries, and it is crucial to provide fast and accurate assessment of vaccination coverage and vaccination gaps to make strategic adjustments promoting vaccine coverage. We reported the effective use of real-time geospatial analysis to identify barriers and gaps in COVID-19 vaccination in a minority population living in South Texas on the US-Mexico Border, to inform vaccination campaign strategies. We developed 4 rank-based approaches to evaluate the vaccination gap at the census tract level, which considered both population vulnerability and vaccination priority and eligibility. We identified areas with the highest vaccination gaps using different assessment approaches. Real-time geospatial analysis to identify vaccination gaps is critical to rapidly increase vaccination uptake, and to reach herd immunity in the vulnerable and the vaccine hesitant groups. Our results assisted the City of Brownsville Public Health Department in adjusting real-time targeting of vaccination, gathering coverage assessment, and deploying services to areas identified as high vaccination gap. The analyses and responses can be adopted in other locations.

Objectives Some pregnant women are not ready or do not want to quit smoking completely, and currently there is no support provided for these women in the UK. Offering help to reduce smoking could reduce the health risks associated with smoking and increase the limited reach of the NHS Stop Smoking Services (SSS) for pregnant women. This study aimed to design and evaluate a hypothetical intervention aimed at pregnant women who are not yet ready or do not want to quit smoking entirely. Methods A hypothetical intervention, the Reduced Smoking During Pregnancy (RSDP) intervention, was conceptualised based on the best available evidence. The intervention was evaluated, using a decision-analytic model developed for SDP interventions. Two different scenarios, a base-case and a cautious-case were developed, and a cost-utility analysis and return on investment analysis were conducted. The uncertainty around the estimates was assessed, using deterministic and probabilistic sensitivity analyses. Results The RSDP intervention could prevent the loss of 13 foetuses and generate 43 quitters 1 year after delivery per 1000 women. In the lifetime analysis, the intervention was cost-effective in both scenarios, with an incremental cost of £363 (95% CI £29 to £672) and 0.44 (95% CI 0.32 to 0.53) QALYs gained in the base-case. Conclusions The study found that the hypothetical reduction intervention would produce significant health benefits, reduce smoking and be cost-effective. Offering pregnant smokers help to reduce smoking could reduce health inequalities, widen the reach of SSS and improve health. This economic evaluation of a novel, intensive intervention could inform the piloting of such interventions.

Objectives In 2019–2020, four national recommendations were published in the United Kingdom to encourage use of low carbon inhalers. This study aimed to investigate whether these were associated with a change in primary care dispensing in England and to explore associations between geographical variation and clinical commissioning group (CCG) characteristics. Design Ecological study using aggregated publicly available data. Setting All CCGs in England (March 2016 to February 2021). Participants not applicable Main outcome measures Percentage of low carbon inhalers dispensed. Results The percentage of low carbon inhalers dispensed was 26.3% in 2020–2021 (of 8.8 million inhalers). This decreased over the study period for short-acting beta-agonist (SABA), inhaled corticosteroid (ICS) and ICS+long-acting beta-agonist (LABA) inhalers. The same trend was seen for LABA and ICS+LABA+long-acting muscarinic antagonist inhalers from 2019. The SABA and ICS classes were less often dispensed as low carbon inhalers (⁓6% versus 35–45%). Interrupted time series analyses found slight increases in low carbon inhaler percentage in the SABA, LABA and ICS classes after April 2019, which were soon erased by the long-term trend. There was also geographical variation, with the north-west, Birmingham and London consistently dispensing more low carbon inhalers. The presence of advice on climate change in CCG formularies/guidelines, the prevalence of asthma and population age profile were associated with significant variation in low carbon inhaler percentage for some classes. Conclusions The percentage of low carbon inhalers dispensed in England remains low and continues to decrease. Greater use of low carbon inhalers is achievable, but is more likely with locally implemented initiatives.