1. Proximal femoral head transcriptome reveals novel candidate genes related to epiphysiolysis in broiler chickens
- Author
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Maurício Egídio Cantão, Ricardo Zanella, Mônica Corrêa Ledur, Jane de Oliveira Peixoto, Fátima Regina Ferreira Jaenisch, I. R. Savoldi, Adriana Mércia Guaratini Ibelli, Luiz Lehmann Coutinho, J. A. P. Marchesi, J. R. C. Pandolfi, P. F. Giachetto, Matthew L. Settles, JANE DE OLIVEIRA PEIXOTO, CNPSA, IGOR RICARDO SAVOLDI, CNPSA, Universidade do Contestado, UDESC-Oeste, ADRIANA MERCIA GUARATINI IBELLI, CNPSA, MAURICIO EGIDIO CANTAO, CNPSA, FÁTIMA REGINA FERREIRA JAENISCH, CNPSA, POLIANA FERNANDA GIACHETTO, CNPTIA, MATTHEW LEE SETTLES, University of California, RICARDO ZANELLA, UPF, JORGE AUGUSTO PETROLI MARCHESI, CNPSA, USP, JOSE RODRIGO CLAUDIO PANDOLFI, CNPSA, LUIZ LEHMANN COUTINHO, Esalq/USP, and MONICA CORREA LEDUR, CNPSA.
- Subjects
Candidate gene ,Placa de crescimento do fêmur ,Genome ,Transcriptome ,0302 clinical medicine ,Necrose da cabeça do fêmur ,Gene Regulatory Networks ,Leg problems ,Genetics ,0303 health sciences ,BCO ,Femur Head ,Epiphysiolysis ,Proximal femoral head separation ,030220 oncology & carcinogenesis ,Epifisiólise ,DNA microarray ,Research Article ,Biotechnology ,lcsh:QH426-470 ,lcsh:Biotechnology ,RNA-sequencing ,Biology ,03 medical and health sciences ,Epiphyses, Slipped ,lcsh:TP248.13-248.65 ,Femur growth plate ,Animals ,Genetic Predisposition to Disease ,Femur ,Bacterial Chondronecrosis with Osteomyelitis ,Gene ,Genetic Association Studies ,Poultry Diseases ,030304 developmental biology ,Femoral Head Necrosis ,Gene Expression Profiling ,Broiler ,Computational Biology ,Reproducibility of Results ,Femoral head necrosis ,Frangos de corte ,lcsh:Genetics ,Gene Ontology ,Broiler chickens ,Sequenciamento de RNA ,RNA ,Chickens - Abstract
BackgroundThe proximal femoral head separation (FHS) or epiphysiolysis is a prevalent disorder affecting the chicken femur epiphysis, being considered a risk factor to infection which can cause bacterial chondronecrosis with osteomyelitis in broilers. To identify the genetic mechanisms involved in epiphysiolysis, differentially expressed (DE) genes in the femur of normal and FHS-affected broilers were identified using RNA-Seq technology. Femoral growth plate (GP) samples from 35-day-old commercial male broilers were collected from 4 healthy and 4 FHS-affected broilers. Sequencing was performed using an Illumina paired-end protocol. Differentially expressed genes were obtained using the edgeR package based on the False Discovery Rate (FDR ResultsApproximately 16 million reads/sample were generated with 2 × 100 bp paired-end reads. After data quality control, approximately 12 million reads/sample were mapped to the reference chicken genome (Galgal5). A total of 12,645 genes were expressed in the femur GP. Out of those, 314 were DE between groups, being 154 upregulated and 160 downregulated in FHS-affected broilers. In the functional analyses, several biological processes (BP) were overrepresented. Among them, those related to cell adhesion, extracellular matrix (ECM), bone development, blood circulation and lipid metabolism, which are more related to chicken growth, are possibly involved with the onset of FHS. On the other hand, BP associated to apoptosis or cell death and immune response, which were also found in our study, could be related to the consequence of the FHS.ConclusionsGenes with potential role in the epiphysiolysis were identified through the femur head transcriptome analysis, providing a better understanding of the mechanisms that regulate bone development in fast-growing chickens. In this study, we highlighted the importance of cell adhesion and extracellular matrix related genes in triggering FHS. Furthermore, we have shown new insights on the involvement of lipidemia and immune response/inflammation with FHS in broilers. Understanding the changes in the GP transcriptome might support breeding strategies to address poultry robustness and to obtain more resilient broilers.
- Published
- 2019