14 results on '"Min‐ji Sung"'
Search Results
2. Proteomic profiling of protein expression changes after 3 months-exercise in ESRD patients on hemodialysis
- Author
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Hye Yun Jeong, Hyun-Ju An, Min Ji Sung, Min Heui Ha, Yu Ho Lee, Dong Ho Yang, Tae Young Yang, Dohyun Han, and So-Young Lee
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Nephrology - Abstract
The prevalence of chronic kidney disease (CKD) is steadily increasing, and it is a global health burden. Exercise has been suggested to improve physical activity and the quality of life in patients with CKD, eventually reducing mortality. This study investigated the change in physical performance after exercise in dialysis-dependent patients with CKD and analyzed differentially expressed proteins before and after the exercise. Plasma samples were collected at enrollment and after 3 months of exercise. Liquid chromatography with tandem mass spectrometry analysis and data-independent acquisition results were analyzed to determine the significantly regulated proteins. A total of 37 patients on dialysis were recruited, and 16 were randomized to exercise for 3 months. The hand grip strength and the walking speed significantly improved in the exercise group. Proteome analysis revealed 60 significantly expressed proteins after 3 months of exercise. In the protein functional analysis, the significantly expressed proteins were involved in the immune response. Also, some of the key significantly expressed proteins [(M Matrix metallopeptidase 9 (MMP-9), Activin A Receptor Type 1B (ACVR1B), Fetuin B (FETUB)] were validated via an enzyme-linked immunosorbent assay. Our results showed that exercise in dialysis-dependent patients with CKD could improve their physical performance. These results indicated that this beneficial effect of exercise in these populations could be associated with immune response.
- Published
- 2023
3. Effects of ICT‐Based Multicomponent Program on Body Composition and Cognitive Function in Older Adults: A Randomized Controlled Clinical Study
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Duri Kim, Jae Hyeok Chang, Seunghwan Song, Young Jin Tak, Up Huh, Jeong Su Cho, Min‐ji Sung, Minwoo Jang, and Jong‐Hwan Park
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Psychiatry and Mental health ,Cellular and Molecular Neuroscience ,Developmental Neuroscience ,Epidemiology ,Health Policy ,Neurology (clinical) ,Geriatrics and Gerontology - Published
- 2022
4. Empagliflozin attenuates diabetic tubulopathy by improving mitochondrial fragmentation and autophagy
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So-Young Lee, Sang Hoon Kim, Dong Ho Yang, Jisu Oh, Dong-Jin Kim, Jun Mo Kang, Jae-Hee Kim, Jin Hyung Heo, Seon Hwa Park, Yu Ho Lee, Sang-Ho Lee, and Min Ji Sung
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Male ,0301 basic medicine ,Physiology ,Gene Expression ,030204 cardiovascular system & hematology ,Pharmacology ,Mitochondrial fragmentation ,Cell Line ,Diabetes Mellitus, Experimental ,Kidney Tubules, Proximal ,Mice ,03 medical and health sciences ,Adenosine Triphosphate ,0302 clinical medicine ,Glucosides ,Sodium-Glucose Transporter 2 ,Tubulopathy ,Autophagy ,medicine ,Empagliflozin ,Animals ,Humans ,Diabetic Nephropathies ,Benzhydryl Compounds ,Sodium-Glucose Transporter 2 Inhibitors ,Organelle Biogenesis ,Chemistry ,medicine.disease ,Adenosine Monophosphate ,Mitochondria ,Mice, Inbred C57BL ,030104 developmental biology ,Apoptosis Regulatory Proteins ,Reactive Oxygen Species ,Cotransporter - Abstract
We examined the effects of empagliflozin, a selective inhibitor of Na+-glucose cotransporter 2, on mitochondrial quality control and autophagy in renal tubular cells in a diabetic environment in vivo and in vitro. Human renal proximal tubular cells (hRPTCs) were incubated under high-glucose conditions. Diabetes was induced with streptozotocin in male C57BL/6J mice. Improvements in mitochondrial biogenesis and balanced fusion-fission protein expression were noted in hRPTCs after treatment with empagliflozin in high-glucose media. Empagliflozin also increased autophagic activities in renal tubular cells in the high-glucose environment, which was accompanied with mammalian target of rapamycin inhibition. Moreover, reduced mitochondrial reactive oxygen species production and decreased apoptotic and fibrotic protein expression were observed in hRPTCs after treatment with empagliflozin, even in the hyperglycemic circumstance. Importantly, empagliflozin restored AMP-activated protein kinase-α phosphorylation and normalized levels of AMP-to-ATP ratios in hRPTCs subjected to a high-glucose environment, which suggests the way that empagliflozin is involved in mitochondrial quality control. Empagliflozin effectively suppressed Na+-glucose cotransporter 2 expression and ameliorated renal morphological changes in the kidneys of streptozotocin-induced diabetic mice. Electron microscopy analysis showed that mitochondrial fragmentation was decreased and 8-hydroxy-2′-deoxyguanosine content was low in renal tubular cells of empagliflozin treatment groups compared with those of the diabetic control group. We suggest one mechanism related to the renoprotective actions of empagliflozin, which reverse mitochondrial dynamics and autophagy.
- Published
- 2019
5. Characteristics of Emotion and Personality in Obstructive Sleep Apnea Patients with Insomnia Symptoms: Analysis of Minnesota Multiphasic Personality Inventory
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Boo Suk Na, Won Chul Shin, Hak Young Rhee, Hyun Keuk Cha, Sang-Beom Kim, Hye Yeon Choi, Min Ji Sung, Ji-Hoon Lee, and Han A Cho
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medicine.medical_specialty ,education.field_of_study ,medicine.diagnostic_test ,business.industry ,Population ,Beck Depression Inventory ,Polysomnography ,medicine.disease ,Obstructive sleep apnea ,Psychasthenia ,Minnesota Multiphasic Personality Inventory ,mental disorders ,Respiratory disturbance index ,medicine ,Insomnia ,medicine.symptom ,Psychiatry ,business ,education - Abstract
Address for correspondence Won Chul Shin, MD, PhD Department of Neurology, Kyung Hee University College of Medicine, Kyung Hee University Hospital at Gangdong, 892 Dongnam-ro, Gangdong-gu, Seoul 05278, Korea Tel: +82-2-440-6166 Fax: +82-2-440-7262 E-mail: shin1chul@gmail.com Objectives: Obstructive sleep apnea-hypopnea syndrome (OSAHS) and insomnia are two of the most common sleep disorders in the general population. Because OSAHS patients with insomnia may have difficulty in adapting to the sleep breathing medical equipment, it is necessary to pay special attention to the diagnosis and treatment of comorbid insomnia. This study is to investigate the emotion and personality in OSAHS patients with insomnia complaints by using Minnesota Multiphasic Personality Inventory-2 (MMPI-2). Methods: We reviewed the results of the standardized questionnaires assessing sleep-related variables, MMPI, and polysomnographic findings of the patients diagnosed as OSAHS. Results: 145 subjects were 49.05±11.83 years of age. The mean Respiratory Disturbance Index was 33.57±19.91 and the mean score of Insomnia Severity Index (ISI) was 11.52±6.49. The mean scores of the Beck Depression Inventory (BDI) and MMPI-2 were within normal ranges. We divided the patients into two groups based on the scores of the ISI, OSAHS with insomnia (n=109) and OSAHS without insomnia (n=36). OSAHS patients with insomnia symptoms had significantly higher scores of hypochondriasis, hysteria, psychasthenia, schizophrenia, paranoia and psychopathic deviate scales and BDI than those without insomnia. Conclusions: Our results suggest that insomnia complaints are very common in OSAHS patients and the psychological problems are more frequently found in OSAHS patients with insomnia symptom than those without it. J Sleep Med 2015;12(2):59-63
- Published
- 2015
6. Chloroquine and amodiaquine enhance AMPK phosphorylation and improve mitochondrial fragmentation in diabetic tubulopathy
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Dong Ho Yang, Seon Hwa Park, Hak Hoon Jun, Hye Yun Jeong, Dong-Jin Kim, Jin Hyung Heo, Min Ji Sung, Sang-Ho Lee, So-Young Lee, and Jun Mo Kang
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Male ,0301 basic medicine ,Mitochondrial ROS ,Enzyme Activators ,lcsh:Medicine ,Amodiaquine ,AMP-Activated Protein Kinases ,Pharmacology ,Mitochondrion ,Article ,Cell Line ,Diabetes Mellitus, Experimental ,Antimalarials ,03 medical and health sciences ,Tubulopathy ,Chloroquine ,medicine ,Animals ,Humans ,Diabetic Nephropathies ,Phosphorylation ,lcsh:Science ,Multidisciplinary ,Chemistry ,lcsh:R ,AMPK ,medicine.disease ,Streptozotocin ,Mitochondria ,Mice, Inbred C57BL ,Glucose ,Kidney Tubules ,030104 developmental biology ,Apoptosis ,lcsh:Q ,medicine.drug - Abstract
We investigated the effects of chloroquine (CQ) and amodiaquine (AQ) on AMPK phosphorylation in renal tubular cells in a diabetic environment in vivo and in vitro. We also examined whether CQ- or AQ-mediated AMPK activity restoration attenuated diabetic tubulopathy by normalizing mitochondrial fragmentation. Human renal proximal epithelial cells (HKC8) were incubated in high-glucose conditions. Diabetes was induced with streptozotocin in male C57/BL6J mice. Treatment with CQ or AQ abolished high-glucose-induced phospho-AMPK and phosph-PGC1α down-regulation in HKC8 cells. Improvements in functional mitochondrial mass and balanced fusion/fission protein expression were observed in HKC8 cells after treatment with CQ or AQ in high-glucose conditions. Moreover, decreased mitochondrial ROS production and reduced apoptotic and fibrotic protein expression were noted in HKC8 cells after treatment with CQ or AQ, even in high-glucose conditions. CQ and AQ treatment effectively mitigated albuminuria and renal histopathologic changes and increased AMPK activity in the kidneys of diabetic mice. Electron microscopy analysis showed that mitochondrial fragmentation was decreased, and 8-OHdG content was low in the renal tubular cells of the CQ and AQ treatment groups compared with those of the diabetic control group. Our results suggest that CQ and AQ may be useful treatments for patients with diabetic kidney disease.
- Published
- 2018
7. Effects of an Indigo Blanket on Insomnia Symptoms: Double Blind, Placebo-Controlled, Randomized Pilot Study
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Kwang Ae Park, Seunghoon Lee, Young Nam Kwon, Jangwoon Park, Han A Cho, Min Ji Sung, Heecheon You, Eunjoo Chang, Won Chul Shin, Eun Jin Jeon, Taeil Ha, and Eunha Kim
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medicine.medical_specialty ,Sleep quality ,business.industry ,Blanket ,Placebo ,Sleep in non-human animals ,Double blind ,Pittsburgh Sleep Quality Index ,Insomnia ,medicine ,Physical therapy ,Sleep diary ,medicine.symptom ,Psychiatry ,business - Abstract
Thirty subjects were recruited in this study and were randomly divided into two groups, indigo blanket and placebo asked to complete the sleep questionnaires [Insomnia Severity Index, Pittsburgh Sleep Quality Index, World Health Organization (WHO)-5 Well-Being Index] and to record the sleep diary before and after they use the blanket. The effects of each blanket was evaluated by sleep quality improvement and sleep satisfaction during their use of blanket, based on the sleep diary and sleep questionnaires.
- Published
- 2014
8. Clinical Characteristics of Elderly Patients Aged over 65 Years with Insomnia Symptoms
- Author
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Hyun Keuk Cha, Young Nam Kwon, Jin San Lee, Min Ji Sung, Han A Cho, Hyo Jung Nam, Ra Young Yoo, and Won Chul Shin
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Pediatrics ,medicine.medical_specialty ,education.field_of_study ,business.industry ,Population ,medicine.disease ,Obstructive sleep apnea ,Circadian rhythm sleep disorder ,medicine ,Insomnia ,Anxiety ,Sleep diary ,Restless legs syndrome ,medicine.symptom ,education ,business ,Depression (differential diagnoses) - Abstract
Address for correspondence Won Chul Shin, MD Department of Neurology, Kyung Hee University College of Medicine, Kyung Hee University Hospital at Gangdong, 892 Dongnam-ro, Gangdong-gu, Seoul 134-727, Korea Tel: +82-2-440-6166 Fax: +82-2-440-7262 E-mail: shinwc@khu.ac.kr Objectives: Sleeping problems and disorders generally increase with aging, and these changes influence elderly functioning and well-being. We studied the clinical characteristics of the elderly patients with insomnia symptoms for the appropriate diagnosis in Korea. Methods: Patients aged over 65 years with insomnia symptoms were recruited from Sleep Center of Kyung Hee University Hospital at Gangdong, from January 2010 to April 2013. General characteristics of sleep patterns, past medical history, previous medication related sleep, sleep diary, and the Minnesota Multiphasic Personality Inventory-2 (MMPI-2) were analyzed to understand the sleep pattern and the clinical characteristics in the elderly patients with insomnia symptoms. Results: The study population included 143 patients (45 men, 98 female), and their mean age was 71.41±5.32 years. These patients were classified according to the cause of insomnia symptoms, the primary cause was 39.1% (92.8% psychophysiological insomnia, 7.2% paradoxical insomnia), the secondary cause was 34.2% (depression, anxiety, associated medical illness, obstructive sleep apnea syndrome, restless legs syndrome), and circadian rhythm sleep disorder was 26.6%. The MMPI-2 profiles were not significant differences in the study population. Conclusions: The clinical characteristics in our study population were different in variable parameters from the general population. It is necessary to understand these differences in the elderly patients for approaching the appropriate diagnosis and medical care. J Korean Sleep Res Soc 2013;10:25-30
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- 2013
9. Blockade of human HERG K+ channels by rosiglitazone, an antidiabetic drug
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Bok Hee Choi, Han Choe, Su Hyun Jo, Gyesik Min, Min Ji Sung, Sang June Hahn, Seungho Lee, and Jimok Kim
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Drug ,ERG1 Potassium Channel ,congenital, hereditary, and neonatal diseases and abnormalities ,Patch-Clamp Techniques ,media_common.quotation_subject ,Long QT syndrome ,hERG ,Pharmacology ,QT interval ,Membrane Potentials ,Rosiglitazone ,Drug Discovery ,Potassium Channel Blockers ,medicine ,Humans ,Hypoglycemic Agents ,cardiovascular diseases ,media_common ,Cardiotoxicity ,biology ,Chemistry ,Osmolar Concentration ,Organic Chemistry ,HEK 293 cells ,medicine.disease ,Ether-A-Go-Go Potassium Channels ,Recombinant Proteins ,Blockade ,Kinetics ,HEK293 Cells ,biology.protein ,Molecular Medicine ,Thiazolidinediones ,medicine.drug - Abstract
This study examined the effect of rosiglitazone, an oral antidiabetic drug, on human ether-a-gogo-related gene (HERG) channels expressed in human embryonic kidney (HEK293) cells. Using the whole-cell patch-clamp technique, interaction between rosiglitazone and HERG in HEK293 cells was studied. Rosiglitazone inhibited HERG channels in a concentration-dependent manner, with an IC 50 value of 18.8 μM and a Hill coefficient of 1.0. These effects were reversible after wash-out of the drug. The rosiglitazone-induced inhibition of HERG channels was voltagedependent, with a steep increase in inhibition over the voltage range of channel opening. However, inhibition was voltage-independent over the voltage range in which channels are fully activated. Rosiglitazone did not change the steady-state activation or inactivation curves or the activation or deactivation kinetics, implying that rosiglitazone blocks HERG channels predominantly in the open and inactivated state rather than in the closed state. The present study suggests that rosiglitazone blocks HERG channels by binding to activated and inactivated channels, and rosiglitazone use should thus be carefully monitored in patients with pre-existing QT prolongation.
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- 2012
10. Blockade of HERG human K+ channels by the antidepressant drug paroxetine
- Author
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Seung Ho, Lee, Min Ji, Sung, Hyang Mi, Lee, Daehyun, Chu, Sang June, Hahn, Su-Hyun, Jo, Han, Choe, and Bok Hee, Choi
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Models, Molecular ,Paroxetine ,HEK293 Cells ,Humans ,Ion Channel Gating ,Antidepressive Agents ,Ether-A-Go-Go Potassium Channels ,Selective Serotonin Reuptake Inhibitors - Abstract
The effects of paroxetine, a selective serotonin reuptake inhibitor, on human ether-a-go-go-related gene (HERG) channels were investigated using the whole-cell patch-clamp technique. The HERG channels were stably expressed in human embryonic kidney cells. Paroxetine inhibited the peak tail currents of the HERG channel in a concentration-dependent manner, with an IC50 value of 0.45 µM and a Hill coefficient of 0.85. These effects were reversible after wash-out of the drug. The paroxetine-induced inhibition of the HERG channels was voltage-dependent. There was a steep increase in inhibition over the voltage range of the channel opening. Also, a shallow voltage-dependent inhibition was detected over the voltage range in which the channels were fully activated. The fractional electrical distance was estimated to be 0.11. Paroxetine induced a leftward shift in the voltage-dependence of the steady-state activation of the HERG channels. Before and after application of the 1 µM paroxetine, the half-maximum activation was -14.21 mV and -27.04 mV, respectively, with no shift in the slope value. The HERG channel block was not use-dependent. The characteristics of the block were dependent on open and inactivated channel states rather than closed state. Paroxetine had no effect on activation and deactivation kinetics, steady-state inactivation. These results suggest that paroxetine blocks the HERG channels by binding to these channels in the open and inactivated states.
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- 2014
11. PS 17-19 CHARACTERISTICS OF EMOTION AND PERSONALITY IN OBSTRUCTIVE SLEEP APNEA PATIENTS WITH INSOMNIA SYMPTOMS
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Jung Ick Byun, Min Ji Sung, Hyun Keuk Cha, Wonchul Shin, and Han A Cho
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Physiology ,business.industry ,media_common.quotation_subject ,medicine.disease ,Obstructive sleep apnea ,Internal Medicine ,medicine ,Insomnia ,Personality ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Clinical psychology ,media_common - Published
- 2016
12. PS 17-80 DIFFERENCE OF METABOLIC DISTURBANCE IN POSITIONAL AND NON-POSITIONAL OBSTRUCTIVE SLEEP APNEA SYNDROME
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Hyun Keuk Cha, Han A Cho, Wonchul Shin, Min Ji Sung, and Jung Ick Byun
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Obstructive sleep apnea ,medicine.medical_specialty ,Physiology ,Metabolic disturbance ,business.industry ,Internal medicine ,Internal Medicine ,medicine ,Cardiology ,Cardiology and Cardiovascular Medicine ,medicine.disease ,business - Published
- 2016
13. Effect of psoralen on the cloned Kv3.1 currents
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Bok Hee Choi, Sang June Hahn, and Min Ji Sung
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Stereochemistry ,Kinetics ,Nerve Tissue Proteins ,CHO Cells ,Transfection ,Membrane Potentials ,chemistry.chemical_compound ,Cricetulus ,Cricetinae ,Drug Discovery ,medicine ,Potassium Channel Blockers ,Animals ,heterocyclic compounds ,Psoralen ,Membrane potential ,biology ,Dose-Response Relationship, Drug ,Furocoumarin ,Chinese hamster ovary cell ,Organic Chemistry ,Ficusin ,Potassium channel blocker ,biology.organism_classification ,Rats ,chemistry ,Shaw Potassium Channels ,Biophysics ,Potassium ,Molecular Medicine ,medicine.drug - Abstract
The psoralen, a furocoumarin derivative, on the cloned neuronal rat Kv3.1 channels stably expressed in Chinese hamster ovary cells was investigated using the whole-cell patch-clamp technique. Psoralen reduced Kv3.1 whole-cell currents in a reversible concentration-dependent manner, with an IC50 value and a Hill coefficient of 2.3 +/- 0.03 microM and 0.9 +/- 0.08, respectively. Psoralen accelerated the decay rate of inactivation of Kv3.1 currents without modifying the kinetics of current activation. The psoralen-induced inhibition of Kv3.1 channels was voltage-dependent, with a steep increase over the voltage range of channel opening. However, the inhibition exhibited voltage independence over the voltage range in which channels are fully activated. Psoralen slowed the deactivation time course, resulting in a tail crossover phenomenon when the tail currents, recorded in the presence and absence of psoralen, were superimposed. Inhibition of Kv3.1 by psoralen was use-dependent at a frequency of 1 Hz. The present results suggest that psoralen acts on Kv3.1 currents as an open-channel blocker.
- Published
- 2008
14. Open channel block of Kv3.1 currents by fluoxetine
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Bok Hee Choi, Min Ji Sung, Sang June Hahn, and Hye Sook Ahn
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medicine.medical_specialty ,Patch-Clamp Techniques ,Time Factors ,Serotonin reuptake inhibitor ,Kinetics ,Nerve Tissue Proteins ,CHO Cells ,Transfection ,Dissociation (chemistry) ,Membrane Potentials ,Reaction rate constant ,Cricetulus ,Internal medicine ,Cricetinae ,Fluoxetine ,medicine ,Potassium Channel Blockers ,Animals ,Patch clamp ,Pharmacology ,Dose-Response Relationship, Drug ,Chemistry ,Chinese hamster ovary cell ,lcsh:RM1-950 ,Rats ,Dose–response relationship ,lcsh:Therapeutics. Pharmacology ,Endocrinology ,Shaw Potassium Channels ,Biophysics ,Molecular Medicine ,Ion Channel Gating ,Selective Serotonin Reuptake Inhibitors ,medicine.drug - Abstract
The action of fluoxetine, a serotonin reuptake inhibitor, on the cloned neuronal rat Kv3.1 channels stably expressed in Chinese hamster ovary cells was investigated using the whole-cell patch-clamp technique. Fluoxetine reduced Kv3.1 whole-cell currents in a reversible, concentration-dependent manner, with an IC50value and a Hill coefficient of 13.4 μ M and 1.4, respectively. Fluoxetine accelerated the decay rate of inactivation of Kv3.1 currents without modifying the kinetics of current activation. The inhibition increased steeply between 0 and +30 mV, which corresponded with the voltage range for channel opening. In the voltage range positive to +30 mV, inhibition displayed a weak voltage dependence, consistent with an electrical distance δ of 0.38. The binding (k+1) and dissociation (k−1) rate constants for fluoxetine-induced block of Kv3.1 were 5.7 μ M−1s−1and 53.5 s−1, respectively. The theoretical KDvalue derived by k− 1/k+1yielded 9.3 μ M. Fluoxetine did not affect the ion selectivity of Kv3.1. Fluoxetine slowed the deactivation time course, resulting in a tail crossover phenomenon when the tail currents, recorded in the presence and absence of fluoxetine, were superimposed. Inhibition of Kv3.1 by fluoxetine was use-dependent. The present results suggest that fluoxetine acts on Kv3.1 currents as an open-channel blocker. Keywords:: fluoxetine, serotonin reuptake inhibitor, Kv3.1, Shaw-type K+channel, open channel block
- Published
- 2008
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