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Uterine microbiota may be involved in reproductive health and disease. This study aims to describe and compare the vaginal and endometrial microbiome patterns between women who became pregnant and women who did not after in vitro fertilization. We also compared the vaginal and endometrial microbiome patterns between women with and without a history of repeated implantation failures (RIF). This pilot prospective cohort study included 48 women presenting to the fertility clinic for IVF from May 2017 to May 2019. Women who achieved clinical pregnancy presented a greater relative abundance of Lactobacillus spp. in their vaginal samples than those who did not (97.69% versus 94.63%; p = 0.027. The alpha and beta diversity of vaginal and endometrial samples were not statistically different between pregnant and non-pregnant women. The Faith alpha diversity index in vaginal samples was lower in women with RIF than those without RIF (p = 0.027). The alpha diversity of the endometrial microbiome was significantly higher in women without RIF (p = 0.021). There were no significant differences in the vaginal and endometrial microbiomes between pregnant and non-pregnant women. The relative abundance of the genera in women with RIF was different from those without RIF. Statistically significant differences in the endometrial microbiome were found between women with and without RIF.

Carbon nanofibers (CNF) can represent a possible alternative to platinum and other graphitic carbonaceous materials for Dye-Sensitized Solar Cell (DSSC) counter electrodes, both from the economic point of view and for what concerns the performance. Carbon nanofibers obtained under different synthesis temperatures were physico-chemically characterized and tested as counter electrode by voltamperometric polarization and impedance measurements under AM 1.5 illumination. The morphological and structural characterization permitted to observe a decrease in surface area and an increase of the crystallinity with the CNF growth temperature. The morphology of these nanofibers is clearly one-dimensional from the electronic microscopy observation, which corresponds to a high electronic conductivity. The best performance as counter electrode in DSSC was found with the CNF characterized by a high surface area and thin nanofilaments.

This article documents the addition of 396 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Anthocidaris crassispina, Aphis glycines, Argyrosomus regius, Astrocaryum sciophilum, Dasypus novemcinctus, Delomys sublineatus, Dermatemys mawii, Fundulus heteroclitus, Homalaspis plana, Jumellea rossii, Khaya senegalensis, Mugil cephalus, Neoceratitis cyanescens, Phalacrocorax aristotelis, Phytophthora infestans, Piper cordulatum, Pterocarpus indicus, Rana dalmatina, Rosa pulverulenta, Saxifraga oppositifolia, Scomber colias, Semecarpus kathalekanensis, Stichopus monotuberculatus, Striga hermonthica, Tarentola boettgeri and Thermophis baileyi. These loci were cross-tested on the following species: Aphis gossypii, Sooretamys angouya, Euryoryzomys russatus, Fundulus notatus, Fundulus olivaceus, Fundulus catenatus, Fundulus majalis, Jumellea fragrans, Jumellea triquetra Jumellea recta, Jumellea stenophylla, Liza richardsonii, Piper marginatum, Piper aequale, Piper darienensis, Piper dilatatum, Rana temporaria, Rana iberica, Rana pyrenaica, Semecarpus anacardium, Semecarpus auriculata, Semecarpus travancorica, Spondias acuminata, Holigarna grahamii, Holigarna beddomii, Mangifera indica, Anacardium occidentale, Tarentola delalandii, Tarentola caboverdianus and Thermophis zhaoermii.

Riassunto Monitoraggio genetico non invasivo della martora (Martes martes) nella pianura padana occidentale: risultati preliminari. Tra il 2003 e il 2004, due esemplari di martora (Martes martes) sono stati investiti da automezzi sulla sponda sinistra del fiume Po (R. N. Garzaia di Valenza, Piemonte). Per accertare la presenza stabile della specie nella Riserva e lungo il tratto vercellese-alessandrino del Parco fluviale del Po, è stato applicato un metodo PCR-RFLP per l’analisi di mtDNA di origine fecale. A partire da gennaio 2007, sono state raccolte 212 feci attribuibili al genere Martes in base a forma e dimensioni. L’analisi di un campione di 117 feci (55%) ha permesso di identificare 10 feci di martora e 15 di faina (M. foina), confermando la presenza della martora nella Riserva e accertandola per la prima volta in un’area collinare in sponda destra del Po (Camino, AL), circa 32 km a monte della Riserva. Resta da valutare se la scarsa resa dell’amplificazione del DNA (21,4%) sia dovuta alla degradazione del materiale genetico o ad un numero elevato di feci di volpe (Vulpes vulpes) erroneamente attribuite a Martes durante la fase di raccolta.

Orculella bulgarica (Hesse 1915) has been recorded from Bulgaria and western Asia, including Turkey, but has also been reported from Spain by several authors. Most studies on this species have been on subfossil shells. Recent findings of living populations in Turkey and Spain have allowed us to report on the reproductive system morphology and mtDNA sequences of this taxon. Despite the apparent lack of conchological differences between specimens from these two geographical areas, this new information revealed the presence of two species. Review of the literature pertaining to Iberian orculids, led us to conclude that the examined population in Spain species must be assigned to Orculella aragonica (Westerlund 1897), and all previous reports of O. bulgarica from the Iberian Peninsula should be ascribed to the former. This species is redescribed and diagnosed herein, highlighting differences between it and O. bulgarica. Some notes about its conservation status and biogeographic origin are also provided.