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Microsatellite locus name, dye used, primer sequences, size range of the amplified product, repeat motif length and the original publication sourced for primer design. Table S2. Microsatellite multiplex name, and the locus marked with each dye. Table S3. GeneMarker panel binning rules, including multiplex design, upper and lower size (bp) boundaries for each marker, allele names, and binning center, minimum, and maximum. Table S4. Pairwise geographical distance (km) and temporal distance (years) between samples from (a) Nguruman, and (b) Ruma. Table S5. Per locus FIS values obtained after testing for deviation from Hardy-Weinberg equilibrium (HWE) using FSTAT v2.9.3 [46] based on 10,000 randomizations for (a) Nguruman and (b) Ruma. Table S6. Pairwise locus P-values after testing for linkage disequilibrium estimated in Genepop v4.1 [43]. Table S7. Per-sample (a) estimates of effective population size (Ne) based on the Jorde/Ryman temporal method showing the interval used in generations (I), the Ne estimate, the 95% CI, and (b) bottleneck results showing the P-values (P) under the two-phase model (TPM), the infinite allele model (IAM) and using the mode-shift test. Figure S1. Allele frequency of each of the 10 microsatellites from (a) Nguruman and (b) Ruma. Figure S2. STRUCTURE [54] estimated Ln likelihood of the data for K values 1â 10 for (a) Nguruman and (b) Ruma, and plots of structure assignment for K values 2â 10 for (c) Nguruman and (d) Ruma. Figure S3. Principal Components Analysis (PCA) of microsatellite data from (a) Nguruman and (b) Ruma. Figure S4. Neighbor-joining tree [57] using Neiâ s genotype frequency based distances (a) Nguruman and (b) Ruma. (DOCX 1088 kb)

Microsatellite locus name, dye used, primer sequences, size range of the amplified product, repeat motif length and the original publication sourced for primer design. Table S2. Microsatellite multiplex name, and the locus marked with each dye. Table S3. GeneMarker panel binning rules, including multiplex design, upper and lower size (bp) boundaries for each marker, allele names, and binning center, minimum, and maximum. Table S4. Pairwise geographical distance (km) and temporal distance (years) between samples from (a) Nguruman, and (b) Ruma. Table S5. Per locus FIS values obtained after testing for deviation from Hardy-Weinberg equilibrium (HWE) using FSTAT v2.9.3 [46] based on 10,000 randomizations for (a) Nguruman and (b) Ruma. Table S6. Pairwise locus P-values after testing for linkage disequilibrium estimated in Genepop v4.1 [43]. Table S7. Per-sample (a) estimates of effective population size (Ne) based on the Jorde/Ryman temporal method showing the interval used in generations (I), the Ne estimate, the 95% CI, and (b) bottleneck results showing the P-values (P) under the two-phase model (TPM), the infinite allele model (IAM) and using the mode-shift test. Figure S1. Allele frequency of each of the 10 microsatellites from (a) Nguruman and (b) Ruma. Figure S2. STRUCTURE [54] estimated Ln likelihood of the data for K values 1â 10 for (a) Nguruman and (b) Ruma, and plots of structure assignment for K values 2â 10 for (c) Nguruman and (d) Ruma. Figure S3. Principal Components Analysis (PCA) of microsatellite data from (a) Nguruman and (b) Ruma. Figure S4. Neighbor-joining tree [57] using Neiâ s genotype frequency based distances (a) Nguruman and (b) Ruma. (DOCX 1088 kb)