1. The clinical significance of PYCR1 expression in renal cell carcinoma
- Author
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Liu Jiayi, Mi Hua, Fu Weijin, Xie Zhibin, Liang Qiumei, Ding Qijian, Chen Yilong, Cheng Jiwen, Liu Deyun, Zheng Shengfeng, and Yang Xiaoli
- Subjects
Male ,Pathology ,medicine.medical_specialty ,renal cell carcinoma ,medicine.medical_treatment ,clinical significance ,Observational Study ,urologic and male genital diseases ,Kidney ,Metastasis ,03 medical and health sciences ,0302 clinical medicine ,Renal cell carcinoma ,Immunochemistry ,medicine ,Carcinoma ,Biomarkers, Tumor ,Humans ,Clinical significance ,030212 general & internal medicine ,RNA, Messenger ,Carcinoma, Renal Cell ,Survival analysis ,business.industry ,General Medicine ,Middle Aged ,medicine.disease ,Prognosis ,Survival Analysis ,Nephrectomy ,Kidney Neoplasms ,Gene Expression Regulation, Neoplastic ,PYCR 1 ,030220 oncology & carcinogenesis ,Female ,Pyrroline Carboxylate Reductases ,business ,Immunostaining ,Research Article - Abstract
Pyrroline-5-carboxylate reductase 1 (PYCR1) is an enzyme involved in cell metabolism and is upregulated in cancer. However, the correlations of PYCR1 expression with the clinicopathological features and prognosis of renal cell carcinoma (RCC) remain unclear. The purpose of this study was to identify the expression of PYCR1 and its clinical relevance in RCC patients. PYCR1 mRNA expression differences between RCC and the adjacent normal renal tissues were assessed using the Cancer Genome Atlas database (TCGA). Subsequently, the expression of PYCR1 mRNA and protein were evaluated by quantitative real-time polymerase chain reaction, Western blot, and immunochemistry using 30 paired frozen samples of RCC and the adjacent normal renal tissues. The protein expression of PYCR1 was evaluated by immunostaining formalin-fixed, paraffin-embedded sections of RCC samples from 96 patients who underwent radical nephrectomy, and its relationship with clinical features were analyzed. Nonpaired t tests were used to statistically analyze the differences between the 2 groups. Cox univariable and multivariable analyses of overall survival (OS) among RCC patients were performed. The expression of PYCR1 mRNA was significantly upregulated in RCC tissues compared to adjacent normal renal tissues in the TCGA database (P
- Published
- 2019