652 results on '"Pause, A"'
Search Results
2. Comparing Fear and Anxiety Chemosignals: Do they modulate facial muscle activity and facilitate identifying facial expressions?
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Nuno Gomes, Bettina M Pause, Monique A M Smeets, and Gün R Semin
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Behavioral Neuroscience ,Physiology ,Physiology (medical) ,Sensory Systems - Abstract
Fear and anxiety are the most frequently studied emotional states in chemosignal research. Despite differences between these two emotional states, findings from research using fear and anxiety body odors (BOs) are often treated as part of a similar phenomenon. In this article, we examine possible similarities and differences between participants exposed to fear and anxiety BOs on 2 dependent variables commonly used in chemosignals’ research: (1) the activation of facial muscles in displays of fear expressions (i.e. the medial frontalis and the corrugator supercilii); and (2) the time required to discriminate between negative emotional expressions (fear, anger, and disgust) and neutral ones. Our results show that fear (vs. rest) and anxiety (vs. exercise) BOs activate the medial frontalis, suggesting that both have a similar impact on receivers’ facial muscles. However, we could not replicate previous findings regarding the influence of fear BOs in discriminating negative emotional faces from neutral ones. Two additional replication attempts failed to replicate the earlier results, indicating that the results reported in the literature with this specific paradigm should be interpreted cautiously. Suggestions for future research examining possible differences between fear and anxiety BOs are advanced.
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- 2023
3. Clinically Designed Improvisatory Music Interventions for Well-Being and Burnout Prevention for Neurology, Psychiatry, and Other Health Professionals: Preliminary Findings (S11.003)
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Clara Takarabe, Rajan Shah, Macy Walesa, Eileen Ngo, Brian Wall, Logan Pause, Gaurava Agarwal, and Borna Bonakdarpour
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- 2023
4. Remote sensing of geomorphodiversity linked to biodiversity—Part III
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Angela Lausch, Michael E. Schaepman, Andrew K. Skidmore, Eusebiu Catana, Lutz Bannehr, Olaf Bastian, Erik Borg, Jan Bumberger, Peter Dietrich, Cornelia Glässer, Jorg M. Hacker, Rene Höfer, Thomas Jagdhuber, Sven Jany, András Jung, Arnon Karnieli, Reinhard Klenke, Toralf Kirsten, Uta Ködel, Wolfgang Kresse, Ulf Mallast, Carsten Montzka, Markus Möller, Hannes Mollenhauer, Marion Pause, Minhaz Rahman, Franziska Schrodt, Christiane Schmullius, Claudia Schütze, Peter Selsam, Ralf-Uwe Syrbe, Sina Truckenbrodt, Michael Vohland, Martin Volk, Thilo Wellmann, Steffen Zacharias, and Roland Baatz
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geomorphic traits ,earth observation ,geomorphodiversity ,remote sensing ,taxonomy ,geodivesity ,traits ,ddc:550 ,structure ,land-use intensity ,ddc:910 ,biodiversity ,functions ,spectral traits ,geomorphology ,550 Geowissenschaften ,ecosystem integrity ,multi-mission ,genesis ,ITC-ISI-JOURNAL-ARTICLE ,terrain ,General Earth and Planetary Sciences ,data science ,ddc:620 ,landforms ,ITC-GOLD - Abstract
Remote sensing (RS) enables a cost-effective, extensive, continuous and standardized monitoring of traits and trait variations of geomorphology and its processes, from the local to the continental scale. To implement and better understand RS techniques and the spectral indicators derived from them in the monitoring of geomorphology, this paper presents a new perspective for the definition and recording of five characteristics of geomorphodiversity with RS, namely: geomorphic genesis diversity, geomorphic trait diversity, geomorphic structural diversity, geomorphic taxonomic diversity, and geomorphic functional diversity. In this respect, geomorphic trait diversity is the cornerstone and is essential for recording the other four characteristics using RS technologies. All five characteristics are discussed in detail in this paper and reinforced with numerous examples from various RS technologies. Methods for classifying the five characteristics of geomorphodiversity using RS, as well as the constraints of monitoring the diversity of geomorphology using RS, are discussed. RS-aided techniques that can be used for monitoring geomorphodiversity in regimes with changing land-use intensity are presented. Further, new approaches of geomorphic traits that enable the monitoring of geomorphodiversity through the valorisation of RS data from multiple missions are discussed as well as the ecosystem integrity approach. Likewise, the approach of monitoring the five characteristics of geomorphodiversity recording with RS is discussed, as are existing approaches for recording spectral geomorhic traits/ trait variation approach and indicators, along with approaches for assessing geomorphodiversity. It is shown that there is no comparable approach with which to define and record the five characteristics of geomorphodiversity using only RS data in the literature. Finally, the importance of the digitization process and the use of data science for research in the field of geomorphology in the 21st century is elucidated and discussed.
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- 2022
5. O Alexandre Magno de Arriano de Nicomédia: o retrato do bom princeps a partir da figura do rei macedônio (século II d.C.)
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Pause, Henrique Hamester
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Masculinidade/Virilidade ,Alexandre Magno ,Arriano de Nicomédia ,Adriano - Abstract
Alexandre Magno é uma das figuras de maior destaque quando pensamos naquelas que são amplamente conhecidas e retratadas no imaginário popular contemporâneo e nos veículos de mídia atuais. Porém, suas representações não são exclusivas dos tempos modernos, sendo também usadas na Antiguidade como parâmetro de comparação, modelo a ser seguido e fonte de inspiração e legitimidade para governantes. Neste artigo, buscamos entender como Arriano de Nicomédia, um autor grego do século II d.C., pensava o papel do princeps romano através de sua obra "Anábase de Alexandre Magno". Acreditamos que, ao representar o rei macedônio enquanto grego, justo e generoso, assim como viril, Arriano visa educar a elite aristocrática romana, em especial a figura do princeps.
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- 2023
6. The Impact of Brexit on EU Renewable Energy and Climate Change Policy
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Jana Nysten and Fabian Pause
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- 2023
7. Filmverzeichnis
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Johannes Pause
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- 2023
8. Inhalt
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Johannes Pause
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- 2023
9. Frontmatter
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Johannes Pause
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- 2023
10. 4. Die Säkularisierung der populistischen Theologie
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Johannes Pause
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- 2023
11. 1. Das Theater des Populismus
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Johannes Pause
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- 2023
12. 3. Die populistische Intervention
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Johannes Pause
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- 2023
13. 2. Die Urszene populistischer Repräsentation
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Johannes Pause
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- 2023
14. Danksagung
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Johannes Pause
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- 2023
15. Literaturverzeichnis
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Johannes Pause
- Published
- 2023
16. Worries and anxiety in parents of adult survivors of childhood cancer: A report from the Swiss Childhood Cancer Survivor Study-Parents
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Salome Christen, Katharina Roser, Erika Harju, Fabienne Gumy‐Pause, Luzius Mader, Janine Vetsch, Andre O. von Bueren, and Gisela Michel
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Oncology ,360 Social problems & social services ,Pediatrics, Perinatology and Child Health ,610 Medicine & health ,Hematology - Abstract
OBJECTIVE Having a child diagnosed with cancer is distressing for parents. We aimed to compare worries and anxiety in parents of adult childhood cancer survivors with parents of the Swiss general population (GP-parents), and to evaluate characteristics associated with worry in parents of survivors. METHODS We conducted a nationwide, population-based study in parents of survivors (survivors aged ≥20 years at study, ≤16 years at diagnosis, >5 years post diagnosis) and GP-parents (≥1 child aged ≥20 years at study). We used the Worry and Anxiety Questionnaire (WAQ), and computed the WAQ total score (worries; possible range 0-80) and caseness for generalized anxiety disorder (anxiety), cognitive, somatic, and any criteria. We used multilevel, multivariable linear regression to identify characteristics associated with worries in parents of survivors. RESULTS We included 787 parents of 513 survivors (41.0% fathers) and 478 GP-parents (42.3% fathers). Parents of survivors and GP-parents did not differ regarding worries (16.6 vs. 17.1, p = .977), anxiety (2.7% vs. 3.6%, p = .536), cognitive (p = .440), and somatic criteria (p = .067). Less parents of survivors met any criteria (17.7% vs. 24.0%, p = .039). Half of parents reported current cancer-related worries. Higher cancer-related worries were reported by mothers (β = 4.1; 95% CI: 2.0-6.2), parents with one child (β = 5.9; 95% CI: 2.0-9.7), currently experiencing disadvantages because of their child's former disease (β = 7.3; 95% CI: 4.0-10.6), or with support needs (β = 9.0; 95% CI: 3.9-14.2; p = .001). CONCLUSIONS It is encouraging that most parents of adult survivors report similar worries and anxiety as GP-parents, but cancer-related worries are still prevalent. Efforts should be made to empower parents to seek psycho-social support if required.
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- 2023
- Full Text
- View/download PDF
17. Impact of Microplastics and Nanoplastics on Livestock Health: An Emerging Risk for Reproductive Efficiency
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Susy Urli, Francesca Corte Pause, Martina Crociati, Anja Baufeld, Maurizio Monaci, and Giuseppe Stradaioli
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microplastics ,nanoplastics ,reproductive system ,health ,bovine ,cow ,cattle ,BPA ,granulosa cells ,steroid hormone ,General Veterinary ,Animal Science and Zoology - Abstract
Pollution due to microplastics and nanoplastics is one of the major environmental issues of the last decade and represents a growing threat to human and animal health. In aquatic species, there is a large amount of information regarding the perturbation of marine organisms; instead, there are only a few studies focusing on the pathophysiological consequences of an acute and chronic exposure to micro- and nanoplastics in mammalian systems, especially on the reproductive system. There are several studies that have described the damage caused by plastic particles, including oxidative stress, apoptosis, inflammatory response, dysregulation of the endocrine system and accumulation in various organs. In addition to this, microplastics have recently been found to influence the evolution of microbial communities and increase the gene exchange, including antibiotic and metal resistance genes. Special attention must be paid to farm animals, because they produce food such as milk, eggs and meat, with the consequent risk of biological amplification along the food chain. The results of several studies indicate that there is an accumulation of microplastics and nanoplastics in human and animal tissues, with several negative effects, but all the effects in the body have not been ascertained, especially considering the long-term consequences. This review provides an overview of the possible adverse effects of the exposure of livestock to micro- and nanoplastics and assesses the potential risks for the disruption of reproductive physiological functions.
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- 2023
18. Populismus und Kino: Politische Repräsentation im Hollywood der 1930er Jahre
- Author
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Johannes Pause
- Subjects
Konservatismus ,Politik ,Kulturgeschichte ,Politische Ideologien ,Populismus ,Politische Repräsentation ,representation ,Medienkultur ,Medienwissenschaft ,political representation ,Great Depression ,Filmwissenschaft ,United States of America ,Media Contents, Content Analysis ,film ,ddc:070 ,Multidisciplinaire, généralités & autres [A99] [Arts & sciences humaines] ,Hollywood ,Exzeptionalismus ,Postfaktischer Populismus ,Multidisciplinary, general & others [A99] [Arts & humanities] ,Film ,USA ,News media, journalism, publishing ,andere Medien ,Repräsentation ,Weltwirtschaftskrise ,Medieninhalte, Aussagenforschung ,Demokratie ,Bildsprache ,Szenographie ,Amerika ,Political Representation ,Democracy ,Visual Language ,Scenography ,Exzeceptionalism ,Postfactual Populism ,Conservatism ,America ,Political Ideologies ,Media Studies ,Other Media ,cultural history ,populism ,Politik im Film ,media culture ,Publizistische Medien, Journalismus,Verlagswesen ,Kino ,Filmanalyse ,film studies ,US-Präsident - Abstract
Die 1930er-Jahre gelten als das populistische Jahrzehnt Hollywoods. Regisseure wie Frank Capra, Leo McCarey und John Ford entwerfen in ihren Werken Szenarien geglückter oder gescheiterter politischer Repräsentation, in denen sich demokratische Ideale mit politischer Theologie und amerikanischem Exzeptionalismus verbinden. Die Szenographie dieser Filme hat sich tief in das kulturelle Gedächtnis der USA eingeschrieben und prägt die politische Inszenierung von Repräsentation bis heute. Johannes Pause liest die damals entstandene Bildsprache als eine Typologie populistischer Repräsentation neu und nutzt sie als Folie, um aktuelle politische Tendenzen zu analysieren.
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- 2023
19. Altura de plantas, número de folhas e perfilhos do capim-tanzânia fertilizado com doses de silicato de sódio em condições de toxidez por alumínio
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Pedro José De Souza Comparin, Elisângela Dupas, Jaqueline Maronez Rosa, Marlene Estevão Marchetti, Alzira Gabriela Da Silva Pause, and Gilmar Gabriel De Souza
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- 2023
20. Genetic background of semen parameters in Italian Simmental bulls
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Alberto Cesarani, Francesca Corte Pause, Jorge Hidalgo, Andre Garcia, Lorenzo Degano, Daniele Vicario, Nicolò P.P. Macciotta, and Giuseppe Stradaioli
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fertility ,Genome-wide association study ,single-step genomic BLUP ,Animal Science and Zoology - Published
- 2023
21. Reservoir-based deterministic loading of single-atom tweezer arrays
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Pause, Lars, Preuschoff, Tilman, Schäffner, Dominik, Schlosser, Malte, and Birkl, Gerhard
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Quantum Physics ,Atomic Physics (physics.atom-ph) ,FOS: Physical sciences ,Quantum Physics (quant-ph) ,Physics - Atomic Physics - Abstract
State-of-the-art individual-atom tweezer platforms so far rely on loading schemes based on spatially superimposing the tweezer array with a cloud of cold atoms created beforehand. Together with immanent atom loss, this dramatically limits the data rate as the application sequence has to be alternated with the time-consuming phases of magneto-optical trapping and laser cooling. We introduce a modular scheme built on an additional cold-atom reservoir and an array of buffer traps effectively decoupling cold-atom accumulation and single-atom supply from the quantum-register operation. For this purpose, we connect a microlens-based tweezer array to a cloud of laser-cooled atoms held in an auxiliary large-focus dipole trap by utilizing atom transport and buffer traps for dedicated single-atom supply. We demonstrate deterministic loading of a hexagonal target structure with atoms solely originating from the reservoir trap. The results facilitate increased data rates and unlock a path to continuous operation of individual-atom tweezer arrays in quantum science making use of discrete functional modules, operated in parallel and spatially separated.
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- 2023
- Full Text
- View/download PDF
22. Loss of hepatic Flcn protects against fibrosis and inflammation by activating autophagy pathways
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Josué M. J. Ramírez-Reyes, Ming Yan, Jennifer L. Estall, Catherine R. Dufour, Arnim Pause, Alain Pacis, Étienne Audet-Walsh, Hyeonju Jeong, Mathieu Paquette, Vincent Giguère, Leeanna El-Houjeiri, Marco Biondini, and Peter M. Siegel
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Liver Cirrhosis ,Biopsy ,Science ,Inflammation ,Diet, High-Fat ,Article ,Hepatitis ,03 medical and health sciences ,Liver disease ,Mice ,0302 clinical medicine ,Fibrosis ,Non-alcoholic Fatty Liver Disease ,Proto-Oncogene Proteins ,medicine ,Autophagy ,Transcription factors ,Animals ,Genetic Predisposition to Disease ,Folliculin ,Liver diseases ,030304 developmental biology ,Mice, Knockout ,0303 health sciences ,Multidisciplinary ,business.industry ,Gene Expression Profiling ,Tumor Suppressor Proteins ,Fatty liver ,Computational Biology ,medicine.disease ,Immunohistochemistry ,3. Good health ,Disease Models, Animal ,Cancer research ,TFEB ,Medicine ,030211 gastroenterology & hepatology ,Disease Susceptibility ,Steatohepatitis ,medicine.symptom ,business ,Transcriptome ,Biomarkers ,Signal Transduction - Abstract
Non-alcoholic fatty liver disease (NAFLD) is the most frequent liver disease worldwide and can progress to non-alcoholic steatohepatitis (NASH), which is characterized by triglyceride accumulation, inflammation, and fibrosis. No pharmacological agents are currently approved to treat these conditions, but it is clear now that modulation of lipid synthesis and autophagy are key biological mechanisms that could help reduce or prevent these liver diseases. The folliculin (FLCN) protein has been recently identified as a central regulatory node governing whole body energy homeostasis, and we hypothesized that FLCN regulates highly metabolic tissues like the liver. We thus generated a liver specific Flcn knockout mouse model to study its role in liver disease progression. Using the methionine- and choline-deficient diet to mimic liver fibrosis, we demonstrate that loss of Flcn reduced triglyceride accumulation, fibrosis, and inflammation in mice. In this aggressive liver disease setting, loss of Flcn led to activation of transcription factors TFEB and TFE3 to promote autophagy, promoting the degradation of intracellular lipid stores, ultimately resulting in reduced hepatocellular damage and inflammation. Hence, the activity of FLCN could be a promising target for small molecule drugs to treat liver fibrosis by specifically activating autophagy. Collectively, these results show an unexpected role for Flcn in fatty liver disease progression and highlight new potential treatment strategies.
- Published
- 2021
23. Differentiating Fear from Anxiety Body Odors – The Finetuning of Chemosignals
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semin, gün, Pause, Bettina, Smeets, Monique, and Gomes, Nuno
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FOS: Psychology ,Psychology ,Social and Behavioral Sciences - Abstract
Social anxiety and fear are often induced experimentally to collect the body odors released while experiencing these emotions (chemosignals). Numerous studies have examined the effects of these chemosignals independently. The measurements of the effects have ranged from psychophysiological, behavioral to cognitive consequences. We plan to examine the similarities and differences between these two chemosignals with different dependent variables (i.e., facial EMG, RT measures, categorization -negative vs. neutral). Social anxiety is a state experienced when somebody's identity is threatened, and one can lose face. Fear, on the other hand, is a state which arises when one's very existence is threatened. The question is how similar or different of the embodied and mental consequences of these chemosignals are. To this end, we plan to conduct an experiment in which participants will be exposed to different facial expressions of emotion as well as a neutral facial expression. As in Kamiloğlu et al. (2008), the participant's task, guided by speed-accuracy instructions, will be to discriminate between emotional and neutral expressions. At the same time, they will be exposed to four chemosignal conditions in a randomized order (fear, social anxiety, rest odor, and physical exercise odor). We shall collect the participants' facial EMG response (i.e., medial frontalis and corrugator supercilii), record their RTs and answers in deciding if the expression is one of emotion or not.
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- 2022
- Full Text
- View/download PDF
24. Der Chor der Heimat
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Johannes Pause
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- 2022
25. Genome Sequences of Microbacterium foliorum Phages Anseraureola, Pondwater, and Yasuo
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David Bamgbowu, John Bsoumai, Jessica Butura, Emma Cady, Gabriella Cholod, Isaac Collibee, Lois Dompreh, Sydney Eisner, Maxim Elmaleh, Katelan Fitzgerald, Eric Gillis, Anna Horgan, Dylan Judd, Julia Keefe, Erika Kovalski, Kylie LaBianca, Patrick Lee, Fengkai Lin, Heather Maiuri, Chloe McDonald, Arden McKnight, Meghan Meseerole, Farah Mizra, Elizabeth Monger, Evelyn Moore, Nina Nguyen, Bettina Noel, Daniel O’Connor, Ruben Pagani, Makenzie Palmgren, Karin Pan, Bondita Pech, Jeanne Qian, Sheida Rastegar, Brittney Simas, Adeline Southard, Matt Tracy, Harry Vuong, Sean Whelan, Angela Zou, Elizabeth Punska, Robert Pause, Fengqiu Zhang, Alexander Ribbe, Peter Chien, and Jessica Rocheleau
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Immunology and Microbiology (miscellaneous) ,Genetics ,Molecular Biology - Abstract
Anseraureola, Pondwater, and Yasuo are bacteriophages with siphovirus morphology that infect Microbacterium foliorum NRRL B-24224. They were isolated from soil collected in Amherst, Massachusetts, and have genome lengths between 17,362 bp and 17,453 bp. These phages each contain 25 predicted protein-coding genes and are assigned to phage cluster EE.
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- 2022
26. O CONSUMO SUSTENTÁVEL A PARTIR DA PERSPECTIVA DOS BRECHÓS REALIZADOS POR ORGANIZAÇÕES VOLUNTÁRIAS, FOMENTANDO A ECONOMIA SOLIDÁRIA
- Author
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Ana Paula de Oliveira Pause
- Published
- 2022
27. Seventh BHD international symposium: recent scientific and clinical advancement
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Mark R. Woodford, Avgi Andreou, Masaya Baba, Irma van de Beek, Chiara Di Malta, Iris Glykofridis, Hannah Grimes, Elizabeth P. Henske, Othon Iliopoulos, Masatoshi Kurihara, Romain Lazor, W. Marston Linehan, Kenki Matsumoto, Stefan J. Marciniak, Yukiko Namba, Arnim Pause, Neil Rajan, Anindita Ray, Laura S. Schmidt, Wei Shi, Ortrud K. Steinlein, Julia Thierauf, Roberto Zoncu, Anna Webb, Mehdi Mollapour, Human genetics, Cancer Center Amsterdam, Marciniak, Stefan [0000-0001-8472-7183], Apollo - University of Cambridge Repository, Woodford, M. R., Andreou, A., Baba, M., van de Beek, I., Di Malta, C., Glykofridis, I., Grimes, H., Henske, E. P., Iliopoulos, O., Kurihara, M., Lazor, R., Linehan, W. M., Matsumoto, K., Marciniak, S. J., Namba, Y., Pause, A., Rajan, N., Ray, A., Schmidt, L. S., Shi, W., Steinlein, O. K., Thierauf, J., Zoncu, R., Webb, A., Mollapour, M., Human Genetics, CCA -Cancer Center Amsterdam, and CCA - Cancer Treatment and Quality of Life
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Birt-Hogg-Dube Syndrome ,Oncology ,Birt-Hogg-Dubé syndrome ,FLCN ,LDHA ,folliculin ,tuberous sclerosis complex ,education ,Humans ,Meeting Report ,health care economics and organizations - Abstract
The 7th Birt-Hogg-Dubé (BHD) International Symposium convened virtually in October 2021. The meeting attracted more than 200 participants internationally and highlighted recent findings in a variety of areas, including genetic insight and molecular understanding of BHD syndrome, structure and function of the tumor suppressor Folliculin (FLCN), therapeutic and clinical advances as well as patients’ experiences living with this malady.
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- 2022
28. Identification of Pre-1950 Synthetic Organic Pigments in Artists’ Paints. A Non-Invasive Approach Using Handheld Raman Spectroscopy
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Inez Dorothé van der Werf, Rika Pause, Klaas Jan van den Berg, and AHM (FGw)
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Archeology ,Computer science ,Materials Science (miscellaneous) ,Nanotechnology ,02 engineering and technology ,Conservation ,01 natural sciences ,symbols.namesake ,royal talens ,synthetic organic pigments ,handheld raman spectroscopy ,microraman spectroscopy ,Actual use ,010401 analytical chemistry ,Non invasive ,Lightfastness ,Mordant ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Identification (information) ,Archaeology ,symbols ,0210 nano-technology ,Raman spectroscopy ,modern artist oil paint ,CC1-960 - Abstract
There is little information on the actual use of early synthetic organic pigments (SOPs) in art objects, especially those from before 1950. Their presence can, however, pose a challenge to conservation because their chemical composition, as well as their lightfastness and sensitivity to solvents, are often unknown. Here, a study on the non-invasive identification of SOPs in historic pre-1950 varnished paint-outs from artists’ materials manufacturer Royal Talens is presented. The paints were analysed using a handheld Raman device. Spectra were evaluated by recording the spectra of the same samples with a benchtop instrument. This study demonstrated that the identification of SOPs in varnished oil paints with a non-invasive approach is possible and rather straightforward. The handheld Raman device allowed us to identify fourteen SOPs from eight pigment classes. Besides the occurrence of expected and the known SOPs of this time period, there were also some surprising results, like the detection of the triarylcarbonium pigments PG2 and PB8, and the monoazo Mordant Yellow 1.
- Published
- 2021
29. Neuroblastoma response to RAS-MAPK inhibitors and APR-246 (eprenetapopt) co-treatment is dependent on SLC7A11 phosphorylation
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Vid Mlakar, Ina Oehme, Laurence Lesne, Sara Najafi, Marc Ansari, and Fabienne Gumy-Pause
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Background We previously demonstrated that APR-246 could be an efficient treatment option against neuroblastoma (NB), the most common pediatric extracranial solid tumor. APR-246’s mechanism of action is not completely understood and can differ between cell types. Here we investigate the involvement of well-known oncogenic pathways in NB’s response to APR-246. Methods A proteome profiler kinase assays and western blot analysis were used to identify the molecular pathways involved in the responses to APR-246. Bulk ATP levels were used to determine the viability of cells and the IC50 for APR-246. Cystine-FITC was used to measure the cellular uptake of cysteine. PmRNA5 was used to affect ERK1/2 and pshRNA1 was used to silence HSP27. An IMR-32 xenograft zebrafish embryo model was used to assess APR-246 and sulfasalazine efficacy in vivo. Results After APR-246 treatment, the most deregulated signaling protein identified was ERK1/2, an end-point kinase of the RAS-MAPK pathway. Induction of phospho-ERK1/2 resulted in increased glutathione (GSH) levels, increased cystine uptake and increased resistance of NB cells to APR-246. Using ERK1/2 inhibitors in combination with APR-246, we were able to categorize cells into synergistic and antagonistic groups. After co-treatment, these two groups differ by their levels of SLC7A11 and Hsp27 phosphorylation, cystine uptake and BIM expression. Using erastin and sulfasalazine, both inhibitors of SLC7A11 and activators of ferroptosis, we were able to reverse the antagonistic effects of ERK1/2 inhibitors and demonstrate a strong synergistic action in vitro and in vivo in zebrafish models. Conclusions These results demonstrated a pivotal role of the RAS-MAPK pathway in the NB cellular response to APR-246 via the modulation of intracellular concentrations of GSH and the transport of cystine, phosphorylation of Hsp27, and programed cell death. Combining APR-246 with RAS-MAPK pathway inhibitors can, in some cases, lead to antagonistic action, which can be reversed by combining APR-246 with the clinically approved drug sulfasalazine.
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- 2022
30. Testicular tissue cryopreservation for fertility preservation in prepubertal and adolescent boys: A 6 year experience from a Swiss multi-center network
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Dehlia Moussaoui, Anna Surbone, Cécile Adam, Tamara Diesch-Furlanetto, Céline Girardin, Julie Bénard, Isabelle Vidal, Fanette Bernard, Kanete Busiah, Thérèse Bouthors, Marie-Pierre Primi, Marc Ansari, Nicolas Vulliemoz, and Fabienne Gumy-Pause
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Pediatrics, Perinatology and Child Health - Abstract
Testicular tissue cryopreservation is the only option of fertility preservation in prepubertal boys. While it is considered experimental, since procedures to obtain mature spermatozoa from prepubertal testicular tissue are still under development, testicular tissue cryopreservation programs have emerged worldwide. Our aim was to study the feasibility and safety of a program of testicular tissue cryopreservation in prepubertal and adolescent boys facing gonadotoxic treatment in three University hospitals in Switzerland. Testicular tissue cryopreservation was accepted by 90% of families, with a total of 35 patients included. The average patient age was 8.5 years (range 7 months to 18.5 years). Malignancies were the most common diagnosis (31 patients, 88.6%) with 16 (45.7%) solid tumors and 15 (42.9%) hematological malignancies. Four (11.4%) patients had a benign condition. The main indication for testicular tissue cryopreservation was conditioning for hematologic stem cell transplantation (25 patients, 71.4%). Testicular tissue was cryopreserved according to the freezing protocol of Louvain Catholic University (Belgium), which includes either only immature testicular tissue freezing, or mature and immature testicular tissue freezing depending on the age of the patient and the presence or absence of haploid cells. The median number of spermatogonia per tubule cross-section was 2 (range 0–6) and spermatozoa were found in only one patient. Tumoral cells were found in one testicular biopsy of a leukemic patient. There were two minor adverse events and none of them required medical treatment or surgical revision. Five patients died during follow-up. Our data demonstrate the feasibility and safety of a program of testicular tissue cryopreservation coordinated by a multidisciplinary team of fertility preservation. Despite the experimental aspect of the procedure, the acceptation rate was high, which highlights the willingness of families and patients to participate in testicular tissue cryopreservation.
- Published
- 2022
31. A Creative Model for an Interdisciplinary Approach to Service-Learning
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Kimberly Pause Tucker, Meghan A Marx, Rivka L. Glaser, and Christine E. Moran
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Service (systems architecture) ,Inservice Training ,Higher education ,Service-learning ,Interdisciplinary Studies ,Plant Science ,010402 general chemistry ,01 natural sciences ,Experiential learning ,Academic department ,ComputingMilieux_COMPUTERSANDEDUCATION ,Humans ,Learning ,Relevance (information retrieval) ,Biology ,Symposium ,Scope (project management) ,010405 organic chemistry ,business.industry ,Workload ,Problem-Based Learning ,Faculty ,0104 chemical sciences ,Animal Science and Zoology ,Engineering ethics ,Curriculum ,Erratum ,business - Abstract
Synopsis The biological sciences are inherently interdisciplinary and important advances in biology cannot be made without collaboration. Despite the increasing emphasis on interdisciplinarity in higher education, science courses only rarely extend to content outside of the STEM discipline. Classes are typically taught by one faculty member in one discipline. To demonstrate relevance while addressing genuine community needs, faculty can use service-learning in their courses. Service-learning is an experiential learning strategy where students learn course content and additional relevant skills through completing service with a community partner. Community needs are frequently beyond the scope of a single course or discipline. In order to better meet community needs, an interdisciplinary collaboration provides a more comprehensive experience that highlights the application and interconnection of course content. This article presents a generalisable model for successful interdisciplinary collaborations. While the nature of course scheduling, academic department structure, and faculty workload can be barriers to collaboration between faculty, they are not insurmountable and accomplishable within this framework. The benefits to the students and the community far outweigh navigating these challenges. Using an interdisciplinary approach in teaching will not only enrich course content and expand student learning in multiple areas, but also increase collaboration within the academy while better meeting community needs.
- Published
- 2021
32. A review of the biological and clinical implications of RAS-MAPK pathway alterations in neuroblastoma
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Edouard Morel, Marc Ansari, Simona Jurkovic Mlakar, Fabienne Gumy-Pause, and Vid Mlakar
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0301 basic medicine ,Cancer Research ,MAP Kinase Signaling System ,RAS-MAPK ,Review ,Transcriptome ,03 medical and health sciences ,Neuroblastoma ,0302 clinical medicine ,RASopathie ,Humans ,Medicine ,Solid tumor ,RC254-282 ,MEK1/2 ,ERK1/2 ,Inhibitors ,business.industry ,Ras mapk ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,030104 developmental biology ,Oncology ,ALK ,Apoptosis ,030220 oncology & carcinogenesis ,Cancer research ,business ,RAS - Abstract
Neuroblastoma is the most common extra-cranial solid tumor in children, representing approximately 8% of all malignant childhood tumors and 15% of pediatric cancer-related deaths. Recent sequencing and transcriptomics studies have demonstrated the RAS-MAPK pathway’s contribution to the development and progression of neuroblastoma. This review compiles up-to-date evidence of this pathway’s involvement in neuroblastoma. We discuss the RAS-MAPK pathway’s general functioning, the clinical implications of its deregulation in neuroblastoma, and current promising therapeutics targeting proteins involved in signaling. Supplementary Information The online version contains supplementary material available at 10.1186/s13046-021-01967-x.
- Published
- 2021
33. Redox Mediators in Microbial Electrochemical Systems
- Author
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André Gemünde, Bin Lai, Laura Pause, Jens Krömer, and Dirk Holtmann
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Electrochemistry ,Catalysis - Published
- 2022
34. AMPK-dependent phosphorylation is required for transcriptional activation of TFEB and TFE3
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Pietri Puustinen, Kurt Dejgaard, Mathieu Paquette, Paola Blanchette, Hyeonju Jeong, Linda C. Zirden, Peter M. Siegel, Arnim Pause, and Leeanna El-Houjeiri
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Transcriptional Activation ,0301 basic medicine ,mTORC1 ,AMP-Activated Protein Kinases ,Biology ,Mice ,03 medical and health sciences ,AMP-activated protein kinase ,Autophagy ,Animals ,Humans ,Phosphorylation ,Protein kinase A ,Molecular Biology ,Transcription factor ,Mechanistic target of rapamycin ,030102 biochemistry & molecular biology ,Basic Helix-Loop-Helix Leucine Zipper Transcription Factors ,AMPK ,Cell Biology ,Fibroblasts ,Cell biology ,030104 developmental biology ,biology.protein ,TFEB ,Lysosomes ,Signal Transduction ,Research Paper - Abstract
Increased macroautophagy/autophagy and lysosomal activity promote tumor growth, survival and chemo-resistance. During acute starvation, autophagy is rapidly engaged by AMPK (AMP-activated protein kinase) activation and MTOR (mechanistic target of rapamycin kinase) complex 1 (MTORC1) inhibition to maintain energy homeostasis and cell survival. TFEB (transcription factor E3) and TFE3 (transcription factor binding to IGHM enhancer 3) are master transcriptional regulators of autophagy and lysosomal activity and their cytoplasm/nuclear shuttling is controlled by MTORC1-dependent multisite phosphorylation. However, it is not known whether and how the transcriptional activity of TFEB or TFE3 is regulated. We show that AMPK mediates phosphorylation of TFEB and TFE3 on three serine residues, leading to TFEB and TFE3 transcriptional activity upon nutrient starvation, FLCN (folliculin) depletion and pharmacological manipulation of MTORC1 or AMPK. Collectively, we show that MTORC1 specifically controls TFEB and TFE3 cytosolic retention, whereas AMPK is essential for TFEB and TFE3 transcriptional activity. This dual and opposing regulation of TFEB and TFE3 by MTORC1 and AMPK is reminiscent of the regulation of another critical regulator of autophagy, ULK1 (unc-51 like autophagy activating kinase 1). Surprisingly, we show that chemoresistance is mediated by AMPK-dependent activation of TFEB, which is abolished by pharmacological inhibition of AMPK or mutation of serine 466, 467 and 469 to alanine residues within TFEB. Altogether, we show that AMPK is a key regulator of TFEB and TFE3 transcriptional activity, and we validate AMPK as a promising target in cancer therapy to evade chemotherapeutic resistance. Abbreviations: ACACA: acetyl-CoA carboxylase alpha; ACTB: actin beta; AICAR: 5-aminoimidazole-4-carboxamide ribonucleotide; AMPK: AMP-activated protein kinase; AMPKi: AMPK inhibitor, SBI-0206965; CA: constitutively active; CARM1: coactivator-associated arginine methyltransferase 1; CFP: cyan fluorescent protein; CLEAR: coordinated lysosomal expression and regulation; DKO: double knock-out; DMEM: Dulbecco’s modified Eagle’s medium; DMSO: dimethyl sulfoxide; DQ-BSA: self-quenched BODIPY® dye conjugates of bovine serum albumin; EBSS: Earle’s balanced salt solution; FLCN: folliculin; GFP: green fluorescent protein; GST: glutathione S-transferases; HD: Huntington disease; HTT: huntingtin; KO: knock-out; LAMP1: lysosomal associated membrane protein 1; MEF: mouse embryonic fibroblasts; MITF: melanocyte inducing transcription factor; MTORC1: MTOR complex 1; PolyQ: polyglutamine; RPS6: ribosomal protein S6; RT-qPCR: reverse transcription quantitative polymerase chain reaction; TCL: total cell lysates; TFE3: transcription factor binding to IGHM enhancer 3; TFEB: transcription factor EB; TKO: triple knock-out; ULK1: unc-51 like autophagy activating kinase 1.
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- 2021
35. Nanomechanical characterisation of a water-repelling terpolymer coating of cellulosic fibres
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Isabelle Pause, Yue Du, Markus Biesalski, Robert W. Stark, Lukas Stühn, Wolfgang Ensinger, Alena K. Bell, Sonja Wendenburg, Marcus Schulze, and Julia Auernhammer
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Chemical Physics (physics.chem-ph) ,Materials science ,Polymers and Plastics ,Scanning electron microscope ,FOS: Physical sciences ,Physics - Applied Physics ,Applied Physics (physics.app-ph) ,engineering.material ,Superhydrophobic coating ,Contact angle ,chemistry.chemical_compound ,symbols.namesake ,chemistry ,Coating ,Wet strength ,Physics - Chemical Physics ,symbols ,engineering ,Cellulose ,Composite material ,Raman spectroscopy ,Nanomechanics - Abstract
Polymer coatings on cellulosic fibres are widely used to enhance the natural fibre properties by improving, for example, the hydrophobicity and wet strength. Here, we investigate the effects of a terpolymer P(S-co-MABP-co-PyMA) coating on cotton linters and eucalyptus fibres to improve the resistance of cellulose fibres against wetness. Coated and uncoated fibres were characterised by using scanning electron microscopy, contact angle measurements, Raman spectroscopy and atomic force microscopy with the objective of correlating macroscopic properties such as the hydrophobicity of the fleece with microscopic properties such as the coating distribution and local nanomechanics. The scanning electron and fluorescence microscopy results revealed the distribution of the coating on the paper fleeces and fibres. Contact angle measurements proved the hydrophobic character of the coated fleece, which was also confirmed by Raman spectroscopy measurements that investigated the water uptake in single fibres. The water uptake also induced a change in the local mechanical properties, as measured by atomic force microscopy. These results verify the basic functionality of the hydrophobic coating on fibres and paper fleeces but call into question the homogeneity of the coating., Comment: 24 pages, 5 figures, submitted
- Published
- 2021
36. Nitrogen dynamics and production of mombaça grass under nitrogen fertilization and cutting heights / Dinâmica do nitrogênio e produção do capim-mombaça sob adubação nitrogenada e alturas de corte
- Author
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Aldi Fernandes de Souza França, Euclides Reuter de Oliveira, Alzira Gabriela da Silva Pause, Jefferson Rodrigues Gandra, Jorge Luís Ferreira, Orlando Filipe Costa Marques, Eliane Sayuri Miyagi Okada, and Elisangela Dupas
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Nitrogen fertilizer ,Animal science ,Dry weight ,Chemistry ,Randomized block design ,General Earth and Planetary Sciences ,chemistry.chemical_element ,Forage ,Plant tissue ,Nitrogen ,General Environmental Science - Abstract
The productive potential, apparent nitrogen conversion efficiency, nitrogen contained in the plant tissue, and apparent nitrogen recovery of Mombasa grass subjected to nitrogen fertilization (0, 100, 300, and 500 kg ha -1 ) and cutting heights were evaluated (0.20 and 0.40 m). The experimental design was a complete Randomized block with a 4 x 2 factorial arrangement, in plots subdivided over time with four replications. The dry mass production (DMP) was determined for the whole plant (PI) and its fractions, and only for the whole plant the apparent nitrogen conversion efficiency (ANCE), contained nitrogen (NC) and apparent nitrogen recovery (ANR). The residual heights evaluated did not interfere (P> 0.05) in the production results and the nitrogen dynamics. An effect (P
- Published
- 2021
37. Nutritional value of Mombasa grass submitted to different grazing heights and nitrogen fertilization / Valor nutritivo do capim-Mombaça submetido a diferentes alturas de pastejo e adubação nitrogenada
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Aldi Fernandes de Souza França, Eliane Sayuri Miyagi Okada, Jefferson Rodrigues Gandra, Alzira Gabriela da Silva Pause, Orlando Filipe Costa Marques, Jorge Luís Ferreira, Euclides Reuter de Oliveira, and Elisangela Dupas
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Animal science ,Nitrogen fertilizer ,Grazing ,General Earth and Planetary Sciences ,Biology ,Value (mathematics) ,General Environmental Science - Published
- 2021
38. Der Kalte Krieg und das Meer
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Johannes Pause
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Hollywood ,Fuel Technology ,Process Chemistry and Technology ,media_common.quotation_subject ,Film studies ,Economic Geology ,Art ,Humanities ,media_common - Published
- 2020
39. Soybean silage in dairy heifers’ diets: ruminal fermentation, intake and digestibility of nutrients
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Caio Seiti Takiya, Cibeli A. Pedrini, Euclides Reuter de Oliveira, Alzira Gabriela da Silva Pause, Isabelle Z. Noia, Giovani Antônio, Giovana S. Urio, J. R. Gandra, Jamille D. O. Batista, Rafael Henrique de Tonissi e Buschinelli de Goes, Anderson P. Acosta, and Tiago A. Del Valle
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animal structures ,Silage ,animal diseases ,0402 animal and dairy science ,food and beverages ,Soil Science ,04 agricultural and veterinary sciences ,Plant Science ,Nutrient intake ,Biology ,040201 dairy & animal science ,Rumen ,Animal science ,Nutrient ,040103 agronomy & agriculture ,Ruminal fermentation ,0401 agriculture, forestry, and fisheries ,Animal Science and Zoology ,Fermentation ,Agronomy and Crop Science - Abstract
This study was designed to determine the effects of soybean silage (SS) in diets of dairy heifers on nutrient intake and digestibility, feeding behaviours, rumen fermentation, N utilisation, and mi...
- Published
- 2020
40. Moving Beyond the Friend-Foe Myth: A Scoping Review of the Use of Social Media in Adolescent and Young Adult Oncology
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Vittoria Espelli, Eva De Clercq, Tamara Diesch, Fabienne Gumy-Pause, Michael Rost, and Bernice Simone Elger
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Ethics ,Medical education ,Adolescent ,business.industry ,Correction ,Context (language use) ,PsycINFO ,CINAHL ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Quality of life (healthcare) ,Systematic review ,Oncology ,Neoplasms ,030220 oncology & carcinogenesis ,Pediatrics, Perinatology and Child Health ,Health care ,Humans ,Medicine ,Social media ,030212 general & internal medicine ,business ,Social Media ,Psychosocial - Abstract
Purpose: Adolescents and young adults (AYA) with cancer present a unique challenge to health care institutions. Their cancer diagnosis and treatment have a profound impact upon their health and well-being. Despite the various support services aimed at improving their quality of life, their needs and preferences are often underestimated or misjudged. Recent studies show that patients are empowered by the knowledge and support they receive online. Given the extensive use of social media among AYA, we aim to identify promises, challenges, and recommendations for integrating these platforms in AYA cancer care. Methodology: We systematically searched seven databases systematically: Scopus, PubMed, PsycInfo, Web of Science, CINAHL, SocINDEX, and Media. We placed no restriction on the type of methodology used in the studies. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses was used to frame the research. Results: Many studies argued that health care professionals need to integrate social media in their clinical practice to engage with patients' lifeworld. Social media were considered important allies in optimizing cancer care at all levels of support, ranging from information provision, treatment adherence, diet and exercise interventions, to professional, peer, and psychosocial self-care. Lack of research on the efficacy of social media in the context of psychosocial support was a commonly cited problem. A small number of publications paid attention to the inherent risks of promoting self-care online. Conclusion: Future studies should continue to pursue empirical research on the efficacy of online psychosocial care, while not neglecting the ethical challenges of social media research.
- Published
- 2020
41. SARS-CoV-2: OF SPANISH DESCENT
- Author
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Sacred Pause
- Subjects
body regions ,viruses ,fungi ,virus diseases ,skin and connective tissue diseases ,SARS-CoV-2, HP2, viral vectors, CRISPR, cDNA, hnRNP Q, gene silencing, CCR5 - Abstract
SARS-CoV-2 is a genetically engineered SARS-COV vector which has been embedded into the backbone of bacteriophage HP2 of Nontypeable Haemophilus Influenza (NTHi). The design of SARS-CoV-2’s spike (S) protein is notable for its ability to facilitate cellular entry via not only the ACE2 receptors but also ICAM-1 which becomes upregulated in a NTHI infection. Technologies used in the development of SARS-CoV-2 were patented by the Spanish National Research Council in 2006 and include methods for creating a SARS-COV vector for gene editing, particularly “gene silencing”, and vaccine administration via “virus particles”. This patent calls for the engineering of a SARS-COV vector using a helper virus such as HP2 for replication and/or transcription. According to this patent, the said system could be constructed using a genome of SARS-COV containing sequences encoding a heterologous reporter gene or a gene derived from a different infectious agent and the construction of a fulllength cDNA, which in the case of SARS-CoV-2 was most likely performed using hnRNP Q CRISPR Activation Plasmid (h) cDNA. Further, NTHI lysates increase exosome release in the human middle ear epithelial cells. Exosomes can regulate gene expression via their miRNAs. SARS-CoV-2 shares a 76% homology with SARS-COV which is an engineering requirement mentioned in the said patent. The patent also sets forth modification principles and sequences for engineering the ORF of gene 7a. This engineering can be observed as a structural analysis has revealed that SARS-CoV-2 ORF 7a has structural homology with ICAM-1. ORF 7a plays a vital role in virus tropism allowing SARS-CoV-2 to be “guided” to gene editing targets via the hosts inflammatory secretions. Manipulation of ORF 7a to evade the hosts natural defenses against virus tropism as well as the use of NTHI lysates can cause an excessive inflammatory response or a “cytokine storm” in high-risk populations who become infected with COVID-19. This mode of engineering would necessarily classify SARS-CoV-2 a biological weapon.
- Published
- 2022
- Full Text
- View/download PDF
42. HSP90 inhibitors induce GPNMB cell-surface expression by modulating lysosomal positioning and sensitize breast cancer cells to glembatumumab vedotin
- Author
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Marco Biondini, Alex Kiepas, Leeanna El-Houjeiri, Matthew G. Annis, Brian E. Hsu, Anne-Marie Fortier, Geneviève Morin, José A. Martina, Isabelle Sirois, Adriana Aguilar-Mahecha, Tina Gruosso, Shawn McGuirk, April A. N. Rose, Unal M. Tokat, Radia M. Johnson, Ozgur Sahin, Eric Bareke, Julie St-Pierre, Morag Park, Mark Basik, Jacek Majewski, Rosa Puertollano, Arnim Pause, Sidong Huang, Tibor Keler, Peter M. Siegel, and Tokat, Ünal Metin
- Subjects
Cancer Research ,Immunoconjugates ,Membrane Glycoproteins ,Cell Line, Tumor ,Cell Membrane ,Genetics ,Antibodies, Monoclonal ,Humans ,Antineoplastic Agents ,Triple Negative Breast Neoplasms ,Lysosomes ,Molecular Biology ,Transcription Factors - Abstract
Transmembrane glycoprotein NMB (GPNMB) is a prognostic marker of poor outcome in patients with triple-negative breast cancer (TNBC). Glembatumumab Vedotin, an antibody drug conjugate targeting GPNMB, exhibits variable efficacy against GPNMB-positive metastatic TNBC as a single agent. We show that GPNMB levels increase in response to standard-of-care and experimental therapies for multiple breast cancer subtypes. While these therapeutic stressors induce GPNMB expression through differential engagement of the MiTF family of transcription factors, not all are capable of increasing GPNMB cell-surface localization required for Glembatumumab Vedotin inhibition. Using a FACS-based genetic screen, we discovered that suppression of heat shock protein 90 (HSP90) concomitantly increases GPNMB expression and cell-surface localization. Mechanistically, HSP90 inhibition resulted in lysosomal dispersion towards the cell periphery and fusion with the plasma membrane, which delivers GPNMB to the cell surface. Finally, treatment with HSP90 inhibitors sensitizes breast cancers to Glembatumumab Vedotin in vivo, suggesting that combination of HSP90 inhibitors and Glembatumumab Vedotin may be a viable treatment strategy for patients with metastatic TNBC.
- Published
- 2022
43. Childhood-Onset Movement Disorders Can Mask a Primary Immunodeficiency: 6 Cases of Classical Ataxia-Telangiectasia and Variant Forms
- Author
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Geraldine Blanchard-Rohner, Anna Peirolo, Ludivine Coulon, Christian Korff, Judit Horvath, Pierre R. Burkhard, Fabienne Gumy-Pause, Emmanuelle Ranza, Peter Jandus, Harpreet Dibra, Alexander Malcolm R. Taylor, and Joel Fluss
- Subjects
Adult ,Male ,Delayed Diagnosis ,Adolescent ,Genotype ,Immunology ,Ataxia Telangiectasia Mutated Proteins ,Diagnosis, Differential ,Ataxia Telangiectasia ,Young Adult ,Immunodeficiency ,Humans ,Immunology and Allergy ,Genetic Testing ,Child ,Cerebellar ataxia ,Movement disorder ,Retrospective Studies ,ddc:616 ,ddc:618 ,Movement Disorders ,Neurodegenerative Diseases ,RC581-607 ,ddc:616.8 ,ATM kinase activity ,Cross-Sectional Studies ,Phenotype ,Child, Preschool ,Mutation ,Ataxia telangiectasia ,Female ,movement disorder ,cerebellar ataxia ,Immunologic diseases. Allergy ,immunodeficiency - Abstract
Ataxia-telangiectasia (A-T) is a neurodegenerative and primary immunodeficiency disorder (PID) characterized by cerebellar ataxia, oculocutaneous telangiectasia, immunodeficiency, progressive respiratory failure, and an increased risk of malignancies. It demands specialized care tailored to the individual patient’s needs. Besides the classical ataxia-telangiectasia (classical A-T) phenotype, a variant phenotype (variant A-T) exists with partly overlapping but some distinctive disease characteristics. Here we present a case series of 6 patients with classical A-T and variant A-T, which illustrates the phenotypic variability of A-T that can present in childhood with prominent extrapyramidal features, with or without cerebellar ataxia. We report the clinical data, together with a detailed genotype description, immunological analyses, and related expression of the ATM protein. We show that the presence of some residual ATM kinase activity leads to the clinical phenotype variant A-T that differs from the classical A-T. Our data illustrate that the diagnosis of the variant form of A-T can be delayed and difficult, while early recognition of the variant form as well as the classical A-T is a prerequisite for providing a correct prognosis and appropriate rehabilitation and support, including the avoidance of diagnostic X-ray procedures, given the increased risk of malignancies and the higher risk for side effects of subsequent cancer treatment.
- Published
- 2022
44. Die zwei Geister des Präsidenten: Lincoln-Mythos und politische Theologie im Hollywood-Kino während der Great Depression: Gabriel over the White House (1933)
- Author
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Pause, Johannes
- Published
- 2022
- Full Text
- View/download PDF
45. Die zwei Geister des Präsidenten. Lincoln-Mythos und politische Theologie im Hollywood-Kino während der Great Depression: Gabriel over the White House (1933)
- Author
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Pause, Johannes
- Subjects
Multidisciplinaire, généralités & autres [A99] [Arts & sciences humaines] ,Populism ,Störung ,Populismus ,Abraham Lincoln ,politische Theologie ,Great Depression ,Film Studies ,Multidisciplinary, general & others [A99] [Arts & humanities] ,USA ,Political Representation - Published
- 2022
46. APENAS ASAS?!!!
- Author
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A. P. O. PAUSE and J. M. S. B. VARGAS
- Published
- 2022
47. Cohort-based association study of germline genetic variants with acute and chronic health complications of childhood cancer and its treatment: Genetic Risks for Childhood Cancer Complications Switzerland (GECCOS) study protocol
- Author
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Nicolas Waespe, Sven Strebel, Tiago Nava, Chakradhara Rao S Uppugunduri, Denis Marino, Veneranda Mattiello, Maria Otth, Fabienne Gumy-Pause, André O Von Bueren, Frederic Baleydier, Luzius Mader, Adrian Spoerri, Claudia E Kuehni, Marc Ansari, University of Zurich, and Ansari, Marc
- Subjects
Adult ,cancer genetics ,610 Medicine & health ,2700 General Medicine ,Cohort Studies ,paediatric clinical genetics & dysmorphology ,Young Adult ,360 Social problems & social services ,Neoplasms ,Humans ,genetics ,Child ,Multimorbidity ,Paediatrics ,General Medicine ,adverse events ,Cross-Sectional Studies ,Germ Cells ,paediatric oncology ,10036 Medical Clinic ,Quality of Life ,Medicine ,clinical pharmacology ,Switzerland - Abstract
IntroductionChildhood cancer and its treatment may lead to various health complications. Related impairment in quality of life, excess in deaths and accumulated healthcare costs are relevant. Genetic variations are suggested to contribute to the wide inter-individual variability of complications but have been used only rarely to risk-stratify treatment and follow-up care. This study aims to identify germline genetic variants associated with acute and late complications of childhood cancer.Methods and analysisThe Genetic Risks for Childhood Cancer Complications Switzerland (GECCOS) study is a nationwide cohort study. Eligible are patients and survivors who were diagnosed with childhood cancers or Langerhans cell histiocytosis before age 21 years, were registered in the Swiss Childhood Cancer Registry (SCCR) since 1976 and have consented to the Paediatric Biobank for Research in Haematology and Oncology, Geneva, host of the national Germline DNA Biobank Switzerland for Childhood Cancer and Blood Disorders (BISKIDS).GECCOS uses demographic and clinical data from the SCCR and the associated Swiss Childhood Cancer Survivor Study. Clinical outcome data consists of organ function testing, health conditions diagnosed by physicians, second primary neoplasms and self-reported information from participants. Germline genetic samples and sequencing data are collected in BISKIDS. We will perform association analyses using primarily whole-exome or whole-genome sequencing to identify genetic variants associated with specified health conditions. We will use clustering and machine-learning techniques and assess multiple health conditions in different models.DiscussionGECCOS will improve knowledge of germline genetic variants associated with childhood cancer-associated health conditions and help to further individualise cancer treatment and follow-up care, potentially resulting in improved efficacy and reduced side effects.Ethics and disseminationThe Geneva Cantonal Commission for Research Ethics has approved the GECCOS study.Research findings will be disseminated through national and international conferences, publications in peer-reviewed journals and in lay language online.Trial registration numberNCT04702321.
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- 2022
48. Simulator-based development of a stability assistant for wheeled excavators
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Valentin Pause, Sebastian Emmerich, Stefan Steidel, René Reinhard, Michael Kleer, Veit Kleeberg, Johannes Weber, and Timo Zenner
- Published
- 2022
49. Phosphorus, Tungsten, and Molybdenum – from Brilliant to Fanal®: Unusual Precipitation Methods of Triphenylmethane Dyes in the Early Twentieth Century
- Author
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Rika Pause, Matthijs de Keijzer, Klaas Jan van den Berg, and AHM (FGw)
- Subjects
Conservation - Abstract
Triphenylmethane based dyes became popular after their invention in 1858 due to their brilliant shades and high tinting strengths and were used not only as colourants for textile and paper dyeing, but also as pigments in artists' materials under the commercial name ‘Fanal®’. However, the extent of their use as pigments in artists’ paints has thus far remained unknown. Despite their bright colours, the characteristic low lightfastness of their dyes and pigments inspired further research for new precipitation methods to increase lightfastness, which ultimately resulted in precipitation using complex salts of phosphorus, tungsten, and molybdenum. The present study is based on archival source research from I.G. Farben, the former producer of Fanal®, and Royal Talens, an artists’ paint manufacturer that used these pigments. This research focuses on the history of developments in precipitation methodologies employed for early triphenylmethane dyes and the development of the various lines of Fanal® pigments in oil paints produced by the Dutch manufacturer Talens in the late 1920s and onwards. Historic literature sources, such as the Talens publication Kunstschildersmaterialen en Schildertechniek by Frits Kerdijk that document the application of Fanal® pigments, are also highlighted.
- Published
- 2022
50. Remote Sensing of Regional Soil Moisture
- Author
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Pause, Marion, Wöhling, Thomas, Schulz, Karsten, Jagdhuber, Thomas, and Schrön, Martin
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regional soil moisture ,remote Sensing ,soil moisture ,radiometer ,radar ,SAR - Published
- 2022
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