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This thesis is focused on the evaluation of changes of structure, dynamics, and development in herb layer situated in natural reservation Dřínová in compare with research which done Karel Drápela. For this purpose has been made 16 phytocenological reléves where were maped spring and summer apect. For my research were used same procedures as during Drapela’s thesis. Changes who signalising homogenisation of herb layer, increase of species who require nitrogen in soil, decrease of specialists species and reduction of sun acess, have been detected through analysis of data files. These changes were apparently caused by covering of tree layer.