50 results on '"Quaranta, V."'
Search Results
2. Macrophages suppress anti-tumour immunity in metastatic pancreatic cancer
- Author
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Quaranta, V and Schmid, Michael
- Published
- 2023
- Full Text
- View/download PDF
3. Chronic rhinosinusitis with nasal polyps: how to identify eligible patients for biologics in clinical practice
- Author
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Gelardi, M., Bocciolini, C., Notargiacomo, M., Schiavetti, I., Lingua, C., Pecoraro, P., Iannuzzi, L., Quaranta, V. A., Giancaspro, R., Ronca, G., Cassano, M., Ciprandi, G., Luca, Amorosa, Flavio, Arnone, Paolo, Luperto, Pasqua Irene Sciancalepore, Luigi, Alonzi, Marilena, Tomaiuolo, Alessandro Maselli Del Giudice, Anna, Miglietta, Veronica, Seccia, Giovanni Giulio Sulis, Cristiano, Caruso, Valentina, Rossi, Ciofalo, Andrea, Consagra, Calogera, Antonio, Palma, and Michela, Mancini
- Subjects
Adult ,Biological Products ,nasal polyp score ,chronic rhinosinusitis with nasal polyps ,biological therapy ,CCG ,SNOT-22 ,adult ,humans ,quality of life ,biological products ,nasal polyps ,rhinitis ,sinusitis ,General Energy ,Nasal Polyps ,Otorhinolaryngology ,Quality of Life ,Humans ,Sinusitis ,Rhinitis - Abstract
This study compared three severity measures for chronic rhinosinusitis with nasal polyps (CRSwNP). The outcome was to identify patients who are eligible for biological therapy.330 adult patients with CRSwNP were examined. Nasal polyp score (NPS), sinonasal outcome test (SNOT-22) and clinical-cytological grading (CCG) were compared. Clinical history, past surgery and asthma control test were also considered.Only 45 (13.6%) patients had a contextual positivity to the three severity measures. The concordance among tests was slight/fair. Patients with severe disease (all tests positive) had more impaired parameters. The mixed cytotype (OR = 4.07), nasal obstruction (OR = 10.06), post-nasal drip (OR = 1.98), embarrassment (OR = 2.53) and difficulty falling asleep (OR = 1.92) were significantly associated with severe CRSwNP.To identify candidates for biological therapy, the contextual use of NPS, SNOT-22 and CCG is preferable. In this way, global assessment of CRSwNP, including morphology, inflammation, comorbidity, symptoms and quality of life is possible.Rinosinusite cronica con poliposi nasale: come individuare i candidati alla terapia biologica nella pratica clinica.Questo studio confrontava 3 differenti indici di gravità della rinosinusite cronica con poliposi nasale (RSCcPN). L’obbiettivo era individuare i pazienti eleggibili al trattamento con farmaci biologici.330 pazienti adulti con RSCcPN erano arruolati. Tutti i pazienti venivano valutati mediante nasal polyp score (NPS), sinonasal outcome test (SNOT-22) e clinical-cytological grading (CCG). Inoltre erano considerati la storia clinica, i pregressi interventi chirurgici ed il controllo dell’asma.Solamente 45 (13,6%) pazienti avevano i 3 indici positivi. La concordanza tra i 3 indici era bassa/modesta. I pazienti con la malattia grave (contestuale positività a tutti i test) avevano peggiori parametri. Il citotipo misto (OR = 4,07), l’ostruzione nasale (OR = 10,06), il post-nasal drip (OR = 1,98), il disagio (OR = 2,53), e la difficoltà ad addormentarsi (OR = 1,92) erano significativamente associati con la malattia grave.È preferibile utilizzare contemporaneamente i 3 test per individuare i pazienti candidati alla terapia biologica. In questo modo si può avere un quadro generale della malattia, comprendente la morfologia dei polipi, lo stato di infiammazione, le comorbidità, i sintomi e la qualità della vita.
- Published
- 2021
4. Clinical-Cytological-Grading and phenotyping in patients with chronic rhinosinusitis with nasal polyps: the relevance in clinical practice
- Author
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Gelardi, M., Porro, G., Quaranta, V., Quaranta, N., Cassano, M., Ciprandi, G., Luigi, Alonzi, Arturo Arnone Caruso, Francesco, Barbara, Michele, Barbara, Paolo, Castelnuovo, Giovanna, Ciancio, Ciofalo, Andrea, Michele, Curatolo, Nicola De Candia, Carlo De Luca, Valentina De Robertis, Valentina Di Benedetto, Valeria, Fiore, Lucia, Iannuzzi, Clara, Imperatore, Cristiano, Lingua, Giuseppe, Lombardo, Alberto, Macchi, Michela, Mancini, Alessandro Maselli del Giudice, Patrizia, Mola, Mario, Notargiacomo, Antonio, Palma, Piero, Pecoraro, Giuseppe, Pellitteri, Krizia, Piccininni, Massimo, Rinaldi, and Valentina, Rossi. Study Group On CRSwNP I.
- Subjects
Pulmonary and Respiratory Medicine ,Adult ,Male ,Allergy ,medicine.medical_specialty ,congenital, hereditary, and neonatal diseases and abnormalities ,real-world ,cytological phenotype ,Chronic rhinosinusitis ,lcsh:Medicine ,Comorbidity ,chronic rhinosinusitis with nasal polyps ,Nasal Polyps ,Internal medicine ,clinical-cytological-grading ,Eosinophilic ,Outcome Assessment, Health Care ,medicine ,Hypersensitivity ,Prevalence ,Humans ,Nasal polyps ,Sinusitis ,Grading (tumors) ,Asthma ,Retrospective Studies ,Aspirin ,business.industry ,lcsh:R ,Cell Biology ,Middle Aged ,medicine.disease ,asthma ,comorbidity ,Clinical Practice ,Cross-Sectional Studies ,Phenotype ,Italy ,Chronic Disease ,Female ,Cardiology and Cardiovascular Medicine ,business - Abstract
Chronic rhinosinusitis (CRS) includes two main phenotypes: without nasal polyps (CRSsNP) and with nasal polyps (CRSwNP). CRSwNP may be associated with comorbidity, mainly concerning asthma, aspirin intolerance, and allergy. CRSwNP patients may also be evaluated by clinical-cytological grading (CCG). The current study investigated the prevalence and characteristics of the different CCG and phenotypes in CRSwNP outpatients examined in clinical practice. This retrospective cross-sectional study enrolled 791 consecutive CRSwNP outpatients (424 males, mean age 48.8 years). In the total population, asthma was a common comorbidity (30.8%) as well as aspirin intolerance (24.8%), and allergy (50.8%). As concerns CCG-grading, 210 (26.5%) outpatients had low-grade, 366 (46.3%) medium, and 215 (27.2%) high. As regards cytological phenotypes, 87 (11%) had neutrophilic type, 371 (46.3%) eosinophilic, 112 (14.2%) mast cell, and 221 (27.9%) mixed. High-grade CCG was significantly associated with more frequent asthma, aspirin intolerance, allergy, recurrent surgery, and mixed cytological phenotype. Low-grade CCG was characterized by fewer comorbidities and operations, and neutrophilic phenotype. Therefore, the present study confirmed that CCG is a useful tool in the management of outpatients with CRSwNP. CRSwNP is frequently associated with asthma, aspirin intolerance, and allergy comorbidity. High-grade CCG is frequently characterized by a mixed cytological phenotype, thus, by more severe progress. These real-world outcomes underline that CRSwNP deserves adequate attention for careful management and optimal identification of the best-tailored therapy; CCG and cytological phenotyping could be fruitful tools in clinical practice. Asthma and aspirin intolerance should be adequately investigated in all CRS patients.
- Published
- 2020
5. The integrin alpha6/beta4 in epithelial and carcinoma cells
- Author
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Quaranta, V, Collo, Luigia Rinalda, Rozzo, C, Starr, L, Gaietta, G, and Tamura, Rn
- Subjects
Integrins ,cell migration ,cell adhesion ,epithelial cells - Abstract
This book represents the most current, comprehensive, and authoritative study of integrins on the market today. It provides an overview of the diverse biological functions of integrins, including: The structure and functions of integrin cytoplasmic domains, the role of b2 integrins in leukocyte adhesion, the role of platelet membrane fibrinogen receptor glycoprotein IIb-IIIa (aIIbb3) in thrombosis and hemostasis, the functions of aV integrin family, the role of integrins in signal transduction, the role of integrins in carcinoma cells, the role of integrins in internalization of microbial pathogens through the binding of Yersinia pseudotuberculosis invasin protein, the role of integrins in the skin, the integrin function in early vertebrate development using amphibian embryos.
- Published
- 2017
6. Prevalence of IgG against hepatitis E virus, Salmonella spp., and Toxoplasma gondii in meat juice samples from wild boars hunted in Southern Italy
- Author
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SARNO, ELEONORA, COSTANZO, NICOLA, SANTORO, ADRIANO MICHELE LUIGI, Quaranta V., Stephan R., Sarno, Eleonora, Costanzo, Nicola, Quaranta, V., Santoro, ADRIANO MICHELE LUIGI, and Stephan, R.
- Published
- 2014
7. Innovative Vibration and Acoustic Methodologies for the Design of Enhanced Composite Panel for Aircraft Applications
- Author
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Marino, A., Vitiello, P., Pagliaroli, T., Quaranta, V., Imperatore, B., Roberto CAMUSSI, Marino, A, Vitiello, P, Pagliaroli, Tiziano, Quaranta, V, Imperatore, B, and Camussi, Roberto
- Published
- 2014
8. Mycotoxin contamination in foodstuffs: decontamination of aflatoxin M1 in bovine milk by clay materials
- Author
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Carraro A.(1), De Giacomo A.(2), Giannossi M.L.(3), Medici L.(3), Muscarella M.(4), Palazzo L.(2), Quaranta V.(2), Summa V.(3), and Tateo F.(1)
- Subjects
food and beverages - Abstract
Mycotoxines are widespread toxic substances produced by moulds in human and animal foodstuffs. Some of them, such as aflatoxin B1 (AFB1), are very toxic even in small amounts, but the modern approach to the problem (and the link with public health) started only in the 60s. The presence of aflatoxin M1 (AFM1), in milk and dairy products is also a problem of crucial interest. AFM1 is resistant to thermal and chemical treatments, so mineral sorbents are highly advisable. Clay minerals are suitable, but only a few data are available, as most studies deal with AFB1 (absent in milk). The capacity of clay materials to remove or attenuate the contamination of AFM1 in bovine milk was studied, also considering the effects of these clays on the nutritional properties of milk (in terms of protein, fat and lactose). In order to make the experiments transferable for practical use, only clays available from the market were tested, as well a very simple clay-milk interaction procedure was used. Some bentonites and a kaolin were selected for the experimental work. In particular, different bentonites were selected in order to partially accomplish the wide crystal-chemical features of smectite. Kaolin was less effective than bentonites, but was still able to detoxify contaminated milk, even using a little amount of kaolin (2.4% of the milk suspension). The detoxification role of the bentonites used was variable, but still very efficient: contaminated bovine milk (up to about 80 ng/L) was purified to safe levels (50ng/L for adults and 25ng/L for lactants), with moderate alteration of the nutritional properties of milk; moreover, the bentonite residue in the purified milk was very low (0.4%). The protein was more sensitive to clay adsorption compared to fat and lactose, and the decrease of protein in the treated milk increased with the clay-milk ratio. Among bentonites (beidellite-montmorillonite, ferruginous and saponite types), a saponite clay showed the highest sorbent capacity, in agreement with theoretical consideration about its higher cell surface (available for AFM1) and higher hydrophobicity. Isothermal adsorption procedures for the two more effective bentonites were also carried out in water. The collected data show an effective and safe use of clays in the detoxification of milk (and dairy products) contaminated by AFM1. The climatic changes observed in the last decades point to a wider diffusion of mycotoxines and health effects, and push toward the search for safe, cheap and accessible food treatments.
- Published
- 2013
9. Preliminary operations for calibrating a phased microphone arrays for air traffic monitoring
- Author
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Petrella, O., Quaranta, V., Ameduri, S., and Giovanni BETTA
- Published
- 2012
10. SLEEP MICROSTRUCTURE IN PATIENTS WITH GASTROESOPHAGEAL REFLUX AND OSAS
- Author
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Maestri, Michelangelo, Bonanni, Enrica, Quaranta, V, Fabbrini, M, DI COSCIO, Elisa, Parisi, M, Rossi, M, Parrino, L, Terzano, Mg, and Murri, Luigi
- Published
- 2010
11. Anticipo e ritardo di fase
- Author
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Bonanni, Enrica, DI COSCIO, Elisa, Quaranta, V, and Choub, A.
- Published
- 2009
12. Ground Vibration Test and Aeroelastic Analyses of the CIRA Unmanned Space Vehicle
- Author
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Belardo, M., Ignazio Dimino, Quaranta, V., Belardo, Marika, I., Dimino, and V., Quaranta
- Subjects
modal analysi ,space vehicle ,ground vibration test - Abstract
This paper deals with the numerical and experimental activities performed by CIRA for the Aeroelastic flutter predictions and the numerical structural model validation of the CIRA Unmanned Space Vehicle in the first phase of the Dropped Transonic Test campaign. Starting from the FE model, the numerical results have been used in order to plan the test strategy and to find the optimal location for sensors and shakers, allowing the optimal control and observation of the structural modes in the frequency range of interest. A dedicated suspension system has been designed and realized in order to achieve the test article free free boundary conditions. The Ground Vibration test has been carried out in order to characterize the experimental dynamic model of the vehicle by using both the Phase Resonance (Normal Mode) and the Phase Separation techniques. A correlation analysis between the experimental modal data and the numerical results has been performed. The aeroelastic model has been updated on the basis of experimental FRFs and modal parameters, by minimizing the discrepancies between the FE model predictions and experimental data. Finally the validated numerical model has been used to investigate the aeroelastic phenomena. The interactions of elastic, inertial and aerodynamic forces have been predicted and the flutter speeds have been calculated.
- Published
- 2009
13. Simulazione di indici a catena (1986-1995) per un confronto numerico con gli indici a base fissa
- Author
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QUARANTA V., DI IORIO, FRANCESCA, Quaranta, V., and DI IORIO, Francesca
- Published
- 1998
14. Il sistema informativo geografico (GIS) nella gestione delle emergenze vetrinarie
- Author
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Quaranta, V., Barbini, E., and Messina, G.
- Published
- 2006
15. UVA and UVB sunscreens microspheres: preparation and characterization
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Miraglia, G., Quaranta, V., Centini, Marisanna, and Anselmi, Cecilia
- Subjects
Sunscreens ,Microspheres - Published
- 2005
16. Tissue remodelling in liver diseases
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Gianluigi Giannelli, Quaranta, V., and Antonaci, S.
- Subjects
Integrins ,6 - Ciencias aplicadas::61 - Medicina [CDU] ,Liver fibrosis - Abstract
Tissue remodelling is a dynamic process that occurs during fetal or adult life and involves a modification of the original organization and function of a tissue. Tissue remodelling is observed in physiological and pathological conditions such as during wound healing or in the mammary gland during the course of pregnancy. In this review we will discuss the remodelling occurring in the liver as a consequence of chronic inflammation, as observed in chronic hepatitis, or as a consequence of hepatocellular carcinoma (HCC) progression in more detail. We will consider how altered deposition and turn-over of extracellular matrix (ECM) proteins could lead to development of liver fibrosis, and how the exacerbation of fibrosis could underlie the development of cirrhosis. The involvement of inflammatory and anti-inflammatory cytokines commonly used as therapeutic agents, such as Interferon-a, is then evaluated with a particular focus on modulation of ECM proteolysis. Finally, we analyze the role of alterations of the surrounding microenvironment including ECM, growth factors, cytokines and membrane receptors for ECM ligands in the development of HCC and in its invasive behaviour.
- Published
- 2003
17. Expression and function of alpha(v)beta(3) and alpha(v)beta(5) integrins in the developing pancreas: roles in the adhesion and migration of putative endocrine progenitor cells
- Author
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Cirulli, V, Beattie, GM, Klier, G, Ellisman, M, Ricordi, C, Quaranta, V, Frasier, F, Ishii, JK, Hayek, A, and Salomon, DR
- Subjects
Adult ,Integrins ,cell migration ,1.1 Normal biological development and functioning ,Fluorescent Antibody Technique ,SCID ,Medical and Health Sciences ,Pancreatic Cancer ,Islets of Langerhans ,Mice ,Rare Diseases ,Underpinning research ,Fetal Tissue Transplantation ,Cell Movement ,Receptors ,Cell Adhesion ,2.1 Biological and endogenous factors ,Animals ,Humans ,Developmental ,Vitronectin ,Aetiology ,Metabolic and endocrine ,Cancer ,Extracellular Matrix Proteins ,pancreatic islets ,alpha(v)beta(3) ,Stem Cells ,Diabetes ,Pancreatic Ducts ,Age Factors ,Epithelial Cells ,endocrine progenitors ,Biological Sciences ,Stem Cell Research ,Gene Expression Regulation ,Stem Cell Research - Nonembryonic - Non-Human ,Pancreas Transplantation ,Digestive Diseases ,Oligopeptides ,Stem Cell Transplantation ,Developmental Biology - Abstract
Cell-cell and cell-matrix interactions play a critical role in tissue morphogenesis and in homeostasis of adult tissues. The integrin family of adhesion receptors regulates cellular interactions with the extracellular matrix, which provides three-dimensional information for tissue organization. It is currently thought that pancreatic islet cells develop from undifferentiated progenitors residing within the ductal epithelium of the fetal pancreas. This process involves cell budding from the duct, migration into the surrounding mesenchyme, differentiation, and clustering into the highly organized islet of Langerhans. Here we report that alpha(v)beta(3) and alpha(v)beta(5), two integrins known to coordinate epithelial cell adhesion and movement, are expressed in pancreatic ductal cells and clusters of undifferentiated cells emerging from the ductal epithelium. We show that expression and function of alpha(v)beta(3) and alpha(v)beta(5) integrins are developmentally regulated during pancreatic islet ontogeny, and mediate adhesion and migration of putative endocrine progenitor cells both in vitro and in vivo in a model of pancreatic islet development. Moreover, we demonstrate the expression of fibronectin and collagen IV in the basal membrane of pancreatic ducts and of cell clusters budding from the ductal epithelium. Conversely, expression of vitronectin marks a population of epithelial cells adjacent to, or emerging from, pancreatic ducts. Thus, these data provide the first evidence for the contribution of integrins alpha(v)beta(3) and alpha(v)beta(5) and their ligands to morphogenetic events in the human endocrine pancreas.
- Published
- 2000
18. Laminin 5 in the human thymus: control of T cell proliferation via alpha6beta4 integrins
- Author
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Vivinus-Nebot, M., Ticchioni, M., Mary, F., Hofman, Paul, Quaranta, V., Rousselle, P., Bernard, A., and Deleage, Gilbert
- Subjects
[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology - Abstract
Laminin 5 (alpha3beta3gamma2) distribution in the human thymus was investigated by immunofluorescence on frozen sections with anti-alpha3, -beta3, and -gamma2 mAbs. In addition to a linear staining of subcapsular basal laminae, the three mAbs give a disperse staining in the parenchyma restricted to the medullary area on a subset of stellate epithelial cells and vessel structures. We also found that laminin 5 may influence mature human thymocyte expansion; while bulk laminin and laminin 2, when cross-linked, are comitogenic with a TCR signal, cross-linked laminin 5 has no effect. By contrast, soluble laminin 5 inhibits thymocyte proliferation induced by a TCR signal. This is accompanied by a particular pattern of inhibition of early tyrosine kinases, including Zap 70 and p59(fyn) inhibition, but not overall inhibition of p56(lck). Using a mAb specific for alpha6beta4 integrins, we observed that while alpha3beta1 are known to be uniformly present on all thymocytes, alpha6beta4 expression parallels thymocyte maturation; thus a correspondence exists between laminin 5 in the thymic medulla and alpha6beta4 on mature thymocytes. Moreover, the soluble Ab against alpha6beta4 inhibits thymocyte proliferation and reproduces the same pattern of tyrosine kinase phosphorylation suggesting that alpha6beta4 is involved in laminin 5-induced modulation of T cell activation.Laminin 5 (alpha3beta3gamma2) distribution in the human thymus was investigated by immunofluorescence on frozen sections with anti-alpha3, -beta3, and -gamma2 mAbs. In addition to a linear staining of subcapsular basal laminae, the three mAbs give a disperse staining in the parenchyma restricted to the medullary area on a subset of stellate epithelial cells and vessel structures. We also found that laminin 5 may influence mature human thymocyte expansion; while bulk laminin and laminin 2, when cross-linked, are comitogenic with a TCR signal, cross-linked laminin 5 has no effect. By contrast, soluble laminin 5 inhibits thymocyte proliferation induced by a TCR signal. This is accompanied by a particular pattern of inhibition of early tyrosine kinases, including Zap 70 and p59(fyn) inhibition, but not overall inhibition of p56(lck). Using a mAb specific for alpha6beta4 integrins, we observed that while alpha3beta1 are known to be uniformly present on all thymocytes, alpha6beta4 expression parallels thymocyte maturation; thus a correspondence exists between laminin 5 in the thymic medulla and alpha6beta4 on mature thymocytes. Moreover, the soluble Ab against alpha6beta4 inhibits thymocyte proliferation and reproduces the same pattern of tyrosine kinase phosphorylation suggesting that alpha6beta4 is involved in laminin 5-induced modulation of T cell activation.
- Published
- 1999
19. Sulla presenza di Brucella Suis Biovar 2 nella lepre in Italia
- Author
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Quaranta, V, Farina, R, Poli, Alessandro, Cerri, Domenico, and Palazzo, L.
- Published
- 1995
20. Gradient of integrin α6A expession in the myocardium during early heart development
- Author
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Collo, Luigia Rinalda, Domanico, S, Klier, G, and AND QUARANTA, V.
- Published
- 1995
21. Psoriatic lesions in patients with chronic liver disease are distinct from psoriasis vulgaris lesions, as judged on basis of integrin adhesion receptors
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Gianluigi Giannelli, Savoia, P., Schiraldi, O., Lospalluti, M., Luca, M., Marchisio, P. C., and Quaranta, V.
- Subjects
Male ,Extracellular Matrix Proteins ,Integrins ,Hepatology ,Liver Diseases ,Mononuclear ,Middle Aged ,Immunohistochemistry ,Diagnosis, Differential ,Diagnosis ,Differential ,Chronic Disease ,Leukocytes ,Leukocytes, Mononuclear ,Humans ,Psoriasis ,Aged ,Epidermis ,Female - Abstract
Psoriatic lesions are relatively frequent in patients with chronic liver disease. Furthermore, therapy with interferons tends to exacerbate the symptoms. The pathogenesis of psoriatic lesions is unclear. An important question is whether such lesions may be linked to the underlying chronic liver disease in these patients, or whether they are incidental manifestations of psoriasis vulgaris. We collected biopsy specimens from involved and uninvolved skin areas of chronic liver disease patients with psoriatic manifestations, as well as from psoriasis vulgaris patients, and investigated the patterns of integrin adhesion receptors by means of immunohistochemical methods. Integrin expression is known to be characteristically altered in psoriasis vulgaris. We found some of these changes in chronic liver disease psoriatic lesions-namely pericellular redistribution and suprabasal expression of the basement membrane receptor alpha 6 beta 4 and of the intercellular integrins alpha 2 beta 1 and alpha 3 beta 1. However, psoriasis vulgaris causes two other typical changes: One is the induction of the prototype fibronectin receptor alpha 5 beta 1, and the other is the alteration of integrin expression in areas of the epidermis that are macroscopically normal. These two changes were not found in chronic liver disease psoriasis biopsy specimens in 14 patients investigated. Thus integrin expression may be useful in differentiating chronic liver disease psoriatic lesions from psoriasis vulgaris lesions. Even though the two types of lesions are indistinguishable on inspection or by their histological features, they may be caused by distinct pathogenetic mechanisms. It remains to be seen whether the underlying chronic liver disease has a role, albeit indirect, in such mechanisms.
- Published
- 1994
22. A monoclonal antibody inhibits adhesion to fibronectin and vitronectin of a colon carcinoma cell line and recognizes the integrins alpha v beta 3, alpha v beta 5, and alpha v beta 6
- Author
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Lehmann, R., Rabenandrasana, C, Tamura, R., Lissitzky, J, Quaranta, V., Pichon, J, Marvaldi, J., LEGOUPIL, Laëtitia, and Aix Marseille Université (AMU)
- Subjects
Integrins ,[SDV]Life Sciences [q-bio] ,Molecular Sequence Data ,Antibodies, Monoclonal ,Cross Reactions ,Receptors, Cytoadhesin ,Cell Line ,Fibronectins ,Molecular Weight ,[SDV] Life Sciences [q-bio] ,Epitopes ,Antigens, Neoplasm ,Cell Movement ,Colonic Neoplasms ,Cell Adhesion ,Tumor Cells, Cultured ,Humans ,Electrophoresis, Polyacrylamide Gel ,Receptors, Vitronectin ,Amino Acid Sequence ,Vitronectin ,Glycoproteins - Abstract
International audience; Using whole viable human colon carcinoma HT29 cells as immunogen, we produced a monoclonal antibody (mAb) termed 69-6-5. The antibody was functionally selected on its anti-cell-spreading activity. By immunoprecipitation of surface radiolabeled cell lysates from HT29-D4 cells (an HT29 cell clone), mAb 69-6-5 recognized a molecular complex resembling integrin heterodimers. Sequential immunodepletions with mAb to the integrin alpha v subunit demonstrated that this complex was composed of alpha v-containing integrins. Accordingly, mAb 69-6-5 reacted with integrin alpha v beta 3 immunopurified from melanoma cells and integrins alpha v beta 5 and alpha v beta 6 immunopurified from pancreatic carcinoma cells. In cell adhesion assays, the 69-6-5 mAb was able to inhibit strongly in a dose-dependent manner arginine-glycine-aspartic acid-mediated adhesion of HT29-D4 cells to vitronectin, fibronectin, or ProNectin F but not to laminin or collagen. Immunoprecipitations with beta chain-specific antisera indicated that these cells express integrins alpha v beta 5 (receptor for vitronectin) and alpha v beta 6 (receptor for fibronectin) but neither alpha v beta 1 nor alpha v beta 3. In summary, these results indicated that mAb 69-6-5 reacts with several alpha v integrins and that it can effectively interfere with the adhesive functions of at least alpha v beta 5 and alpha v beta 6, which represent the major receptors on HT29-D4 cells responsible for their adhesion on vitronectin and fibronectin.
- Published
- 1994
23. A new isoform of the laminin receptor α7β1 is developmentally regulated in skeletal muscle
- Author
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Collo, Luigia Rinalda, Starr, L, and Quaranta, V.
- Published
- 1993
24. Cell-type specific integrin variants with alternative α chain cytoplasmic domain
- Author
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Tamura, R. N., Cooper, H. M., Collo, Luigia Rinalda, and Quaranta, V.
- Published
- 1991
25. Ia-like antigens on human T lymphocytes: relationship to other surface markers, role in mixed lymphocyte reactions, and structural profile
- Author
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Indiveri, F., Wilson, B. S., Russo, C., Quaranta, V., Pellegrino, M. A., and Ferrone, S.
- Subjects
Immunology ,Immunology and Allergy - Published
- 1980
26. Additive layer manufacturing for entry capsules
- Author
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Gardi, R., Giuseppe Pezzella, Franchitti, S., Borrelli, R., Pirozzi, C., Quaranta, V., Tiseo, B., Bruno, G., R. Gardi, G. Pezzella, S. Franchitti, R. Borrelli, C. Pirozzi, V. Quaranta, B. Tiseo, G. Bruno, International Astronautical Federation, IAF, Gardi, R., Pezzella, G., Franchitti, S., Borrelli, R., Pirozzi, C., Quaranta, V., Tiseo, B., and Bruno, G.
27. Altered expression of basement membrane proteins and their integrin receptors in lichen planus: Possible pathogenetic role of gelatinases A and B
- Author
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Giannelli, G., Brassard, J., Caterina Foti, Stetler-Stevenson, W. G., Falk-Marzillier, J., Zambonin-Zallone, A., Schiraldi, O., and Quaranta, V.
28. Acoustic antenna calibration accuracy: A parametric investigation
- Author
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Petrella, O., Quaranta, V., Ameduri, S., and Giovanni BETTA
29. Integrin distributions in renal cell carcinomas of various grades of malignancy
- Author
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Korhonen M, Laitinen L, Jari Ylänne, Gk, Koukoulis, Quaranta V, Juusela H, Ve, Gould, and Virtanen I
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Integrins ,Fluorescent Antibody Technique ,Humans ,Collagen ,Laminin ,Carcinoma, Renal Cell ,Immunohistochemistry ,Basement Membrane ,Kidney Neoplasms ,Research Article - Abstract
We studied 41 renal cell carcinomas, classified according to histologic grades G1 through G3, by indirect immunofluorescence microscopy using a panel of monoclonal antibodies (MAb) against various integrin subunits, and the basement membrane (BM) components laminin and collagen type IV. Selected cases also were immunostained using the avidin-biotin-complex method. The alpha 3 and beta 1 integrin subunits were detected in tumor cells of all the carcinomas. All G1 carcinomas, like normal tubular epithelial cells, expressed the alpha 6 subunit, whereas it was lacking in 20% and 40% of G2 and G3 carcinomas, respectively. Furthermore, when alpha 6 was expressed, a lack of basally polarized organization of the subunit, coupled with disorganization of the BM components, correlated with histologic grade. Another feature that appeared to characterize the more anaplastic tumors was their high level (80%) of the alpha v subunit expression as compared with its absence in the G1 carcinomas. Stromal myofibroblasts, identified by double-labeling with anti-myosin, were often characterized by the expression of the alpha 1, alpha 3, alpha 5 and beta 1 subunits. These results indicate that changes in integrin expression in renal cell carcinomas may be correlated with their degree of histologic malignancy.
30. Pass-by noise tests by means of CIRA acoustic antennas system
- Author
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Diodati, G., Quaranta, V., Fiorillo, V., Tarallo, F., and Francesco Camastra
- Subjects
Acoustic antenna ,Acoustic source detection and localization ,Beam-forming ,Acoustics and Ultrasonics
31. Severe anthrax outbreaks in Italy in 2004: considerations on factors involved in the spread of infection
- Author
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Fasanella, A., Garofolo, G., Domenico Galante, Quaranta, V., Palazzo, L., Lista, F., Adone, R., and Jones, M. H.
32. An oil-emulsion vaccine induces full-protection against Mycoplasma agalactiae infection in sheep
- Author
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Buonavoglia D, Greco G, Quaranta V, Corrente M, Martella V, and Nicola Decaro
33. Proteolytic processing of laminin-5 by MT1-MMP in tissues and its effects on epithelial cell morphology
- Author
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Koshikawa, N., Schenk, S., Gilbert Moeckel, Sharabi, A., Miyazaki, K., Gardner, H., Zent, R., and Quaranta, V.
34. Cooperative interactions of laminin 5 gamma2 chain, matrix metalloproteinase-2, and membrane type-1-matrix/metalloproteinase are required for mimicry of embryonic vasculogenesis by aggressive melanoma
- Author
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Re, Seftor, Ea, Seftor, Koshikawa N, Ps, Meltzer, Lm, Gardner, Martin Bilban, Wg, Stetler-Stevenson, Quaranta V, and Mj, Hendrix
- Subjects
Uveal Neoplasms ,Skin Neoplasms ,Matrix Metalloproteinases, Membrane-Associated ,Neovascularization, Pathologic ,Molecular Mimicry ,Tumor Cells, Cultured ,Humans ,Matrix Metalloproteinase 2 ,Metalloendopeptidases ,Oligonucleotides, Antisense ,Cell Adhesion Molecules ,Melanoma ,Oligonucleotide Array Sequence Analysis - Abstract
Vasculogenic mimicry describes a process where aggressive tumor cells in three-dimensional matrices mimic embryonic vasculogenesis by forming extracellular matrix (ECM)-rich, patterned tubular networks. Microarray gene chip analyses revealed significant increases in the expression of laminin 5 (Ln-5, gamma2 chain) and matrix metalloproteinases (MMP)-1, -2, -9, and MT1-MMP (MMP-14) in aggressive compared with poorly aggressive melanoma cells. These components colocalized with developing patterned networks and antisense oligonucleotides to the Ln-5 gamma2 chain (but not sense oligonucleotides), and antibodies to MMP-2 or MT1-MMP (but not MMP-9) inhibited the formation of these networks. Cultures which did not receive antibodies to either MMPs-2 or -14 contained the Ln-5 gamma2 chain promigratory cleavage fragments. Poorly aggressive melanoma cells seeded on collagen I matrices preconditioned by the aggressive cells formed tubular networks along the Ln-5 gamma2 chain-enriched tracks deposited by the aggressive cells. These results suggest that increased expression of MMP-2 and MT1-MMP, along with matrix deposition of the Ln-5 gamma2 chain and/or its cleavage fragments, are required for vasculogenic mimicry by aggressive melanoma cells. Furthermore, the apparent recapitulation of laminin-rich, patterned networks observed in aggressive melanoma patients' tissue sections by aggressive melanoma tumor cells in three-dimensional culture may also serve as a model to help identify specific molecular targets which could function as templates for the coordinated migration of aggressive tumor cells and their proteolytic remodeling of the ECM and may have profound implications for the development of novel therapies directed at the ECM to alter tumor progression.
35. Acoustic spectrum characterization of a forest fire event
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Domingos Viegas, Sorge, S., Tassini, C. C., D Altrui, G., Quaranta, V., and Dimino, I.
36. Standardization procedure for the nasal nitric oxide measurement method using Niox MINO® and the tidal-breathing technique with velum-closure
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Gelardi, M., Abbattista, G., Quaranta, V. N., Quaranta, N., Seccia, V., Buttafava, S., Frati, F., and Giorgio Ciprandi
37. Acoustic antennas characterization for pass-by-noise tests
- Author
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Diodati, G., Quaranta, V., Tarallo, F., and Francesco Camastra
38. Human osteoclast-like cells selectively recognize laminin isoforms, an event that induces migration and activates Ca2+ mediated signals
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Colucci, S., Giannelli, G., Maria Grano, Faccio, R., Quaranta, V., and Zallone, A. Z.
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Integrins ,Cell Movement ,Chemotaxis ,Stem Cells ,Cell Adhesion ,Humans ,Osteoclasts ,Calcium ,Cell Biology ,Laminin ,Cytoskeleton ,Cell Line ,Signal Transduction - Abstract
Osteoclast precursors are chemotactically attracted to sites of bone resorption via migration pathways that include transendothelial crossing in blood capillaries. Transendothelial migration involves poorly understood interactions with basal lamina molecules, including laminins. To investigate osteoclast-laminin interactions, we used human osteoclast-like cell lines obtained from giant cell tumors of bone (GCT 23 and GCT 24). These cell lines are a well-characterized model for osteoclast functions, such as bone resorption and the behaviour of osteoclast precursors. Both GCT cell lines adhered to laminin-2 (merosin) coated wells in standard adhesion assays, but failed to adhere to laminin-1 (EHS-laminin). By light microscopy, GCT cells on laminin-2 were partially spread, with a motile morphology. None of the anti-integrin antibodies tested inhibited GCT cells adhesion to laminin-2. Peptides containing the integrin adhesion site RGD or the laminin adhesion sequence IKVAV did not inhibit GCT cell adhesion to laminin-2. By immunofluorescence, beta 1 integrins were organized in focal adhesions. However, in the presence of monensin this reorganization of beta 1 integrins was abolished, indicating that it was probably due to secretion of fibronectin by GCT cells subsequent to adhesion to laminin-2. GCT cells transmigrated through membranes coated with laminin-2, much more efficiently than through membranes coated with collagen. Migration was induced by osteocalcin, as a chemoattractant, in a dose-dependent manner. At low osteocalcin concentrations, transmigration was detectable on laminin-2 but not collagen. In cells loaded with fura-2, a sharp increase in intracellular Ca2+ was detected upon addition of soluble laminin-2, but not laminin-1, due to release from thapsigargin-dependent intracellular stores. In summary, osteoclasts may recognize laminin isoforms differentially. Initial adhesion to laminin-2 appears to be due to integrin-independent mechanisms. Such adhesion, though, may trigger secretion of fibronectin that could then support spreading and efficient chemotactic migration. These mechanisms may play an important role in facilitating chemotactic migration of osteoclast precursors toward the bone surface.
39. Distribution of the alpha 1-alpha 6 integrin subunits in human developing and term placenta
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Korhonen M, Jari Ylänne, Laitinen L, Hm, Cooper, Quaranta V, and Virtanen I
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Integrins ,Microvilli ,Placenta ,Delivery, Obstetric ,Capillaries ,Trophoblasts ,Pregnancy Trimester, First ,Microscopy, Fluorescence ,Pregnancy ,Pregnancy Trimester, Second ,Decidua ,Humans ,Female ,Tissue Distribution ,Endothelium, Vascular - Abstract
The distribution of the alpha 1-alpha 6 as well as alpha v, beta 1, beta 3 and beta 4 integrin subunits in human first and second trimester and term placentas was studied by indirect immunofluorescence microscopy using a panel of monoclonal antibodies (mAbs). In first and second trimester villi, the alpha 1 and beta 1 integrin subunits were detected in the stromal cells, that were mostly also immunoreactive for desmin. Desmin-positive stromal cells were also found in villi of term placentas, but the stroma was negative for anti-alpha 1 and -beta 1. In the villous trophoblast, anti-alpha 6 and -beta 4 revealed a distinct basal immunoreactivity during all stages of development, whereas immunoreactivity for the alpha 3 and beta 1 subunits emerged during the second and third trimesters. Throughout placental development, endothelia of villous capillaries reacted prominently with anti-alpha 1 and -beta 1. Intermediate trophoblastic cells displayed a somewhat heterogenous immunoreactivity for the beta 1, alpha 1, alpha 3 and alpha 5 integrin subunits, and differed from villous trophoblast also in their lack of expression of the alpha 6 and beta 4 subunits. While nondecidualized endometrial cells displayed weak reactivity for the alpha 1 and beta 1 integrin subunits, the individual decidual cells presented both a basement membrane and a cell surface-confined immunoreactivity for anti-alpha 1, -alpha 3, and -beta 1. The results suggest a role for integrins in placental development, and show that expression of integrins is modulated during the differentiation of trophoblast, villous stroma, and decidual cells. Furthermore, the basal localization of alpha 6 beta 4 and alpha 3 beta 1 integrins suggests that they are employed as basement membrane receptors in the villous trophoblast, and the emergence of the alpha 3 beta 1 complex may reflect that the cytotrophoblast and syncytiotrophoblast recognize the basement membrane differently.
40. A new isoform of the laminin receptor integrin α7β1 is developmentally regulated in skeletal muscle
- Author
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Ginetta COLLO, Starr, L., and Quaranta, V.
41. FEM analysis of RF breast ablation: Multiprobe versus cool-tip electrode
- Author
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Quaranta V, Manenti G, Bolacchi F, Cossu E, Ca, Pistolese, Oreste Claudio Buonomo, Carotenuto L, Piconi C, and Simonetti G
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RFA ,RF ablation ,finite element analysis ,surgical technique ,animal tissue ,Body Temperature ,Mammary Glands, Animal ,Settore MED/36 - Diagnostica per Immagini e Radioterapia ,multi-probe ,Animals ,Humans ,controlled study ,human ,Breast ,intermethod comparison ,Electrodes ,cool-tip electrode ,FEM ,nonhuman ,Animal ,article ,breast surgery ,electrode ,Mammary Glands ,human tissue ,Breast ablation ,priority journal ,Liver ,finite-element method ,histopathology ,radiofrequency ablation ,Catheter Ablation ,Cattle ,Female - Abstract
Radio-frequency ablation (RFA) has recently received much attention as an effective minimally invasive strategy for the local treatment of tumors. The purpose of this study was to evaluate the efficacy of single-needle cool-tip RF breast ablation in terms of temperature distribution and duration of the procedure as compared to multiprobe RF breast ablation.Two different commercially available radiofrequency ablation needle electrodes were compared. Finite-element method (FEM) models were developed to simulate the thermoablation procedures. A series of ex vivo radiofrequency thermal lesions were induced to check the response of the FEM calculations.Data obtained from FEM models and from ex vivo procedures showed that cool-tip RF breast ablation assures better performances than multiprobe RF breast ablation in terms of temperature distribution and duration of the procedure. Histopathological analysis of the cool-tip RF thermoablated specimens showed successful induction of coagulation necrosis in the thermoablated specimens.Data obtained from FEM models and from ex vivo procedures suggest that the proposed cool-tip RF breast ablation may kill more tumor cells in vivo with a single application than the multiprobe RF breast ablation.
42. Sleepiness and sleep disturbances in an elderly population
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Bonanni, E., Tognoni, G., Fabbrini, M., Maestri, M., Di Coscio, E., Quaranta, V., Sposito, R., Salvati, N., Gabriele Siciliano, and Murri, L.
43. Patterns of transcription factor programs and immune pathway activation define four major subtypes of SCLC with distinct therapeutic vulnerabilities
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Carl M. Gay, C. Allison Stewart, Elizabeth M. Park, Lixia Diao, Sarah M. Groves, Simon Heeke, Barzin Y. Nabet, Junya Fujimoto, Luisa M. Solis, Wei Lu, Yuanxin Xi, Robert J. Cardnell, Qi Wang, Giulia Fabbri, Kasey R. Cargill, Natalie I. Vokes, Kavya Ramkumar, Bingnan Zhang, Carminia M. Della Corte, Paul Robson, Stephen G. Swisher, Jack A. Roth, Bonnie S. Glisson, David S. Shames, Ignacio I. Wistuba, Jing Wang, Vito Quaranta, John Minna, John V. Heymach, Lauren Averett Byers, Gay, C. M., Stewart, C. A., Park, E. M., Diao, L., Groves, S. M., Heeke, S., Nabet, B. Y., Fujimoto, J., Solis, L. M., Lu, W., Xi, Y., Cardnell, R. J., Wang, Q., Fabbri, G., Cargill, K. R., Vokes, N. I., Ramkumar, K., Zhang, B., Della Corte, C. M., Robson, P., Swisher, S. G., Roth, J. A., Glisson, B. S., Shames, D. S., Wistuba, I. I., Wang, J., Quaranta, V., Minna, J., Heymach, J. V., and Byers, L. A.
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0301 basic medicine ,Cancer Research ,Lung Neoplasms ,Prognosi ,medicine.medical_treatment ,Mice, Nude ,Biology ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Cell Line, Tumor ,medicine ,Animals ,Humans ,neuroendocrine ,neoplasms ,Transcription factor ,Cisplatin ,Kinase ,Animal ,ASCL1 ,EMT ,Immunity ,SCLC ,POU2F3 ,Immunotherapy ,Gene signature ,Prognosis ,Small Cell Lung Carcinoma ,humanities ,respiratory tract diseases ,Gene Expression Regulation, Neoplastic ,Lung Neoplasm ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,NEUROD1 ,intratumoral heterogeneity ,Cancer research ,Female ,medicine.drug ,Human ,Transcription Factors - Abstract
Despite molecular and clinical heterogeneity, small cell lung cancer (SCLC) is treated as a single entity with predictably poor results. Using tumor expression data and non-negative matrix factorization, we identify four SCLC subtypes defined largely by differential expression of transcription factors ASCL1, NEUROD1, and POU2F3 or low expression of all three transcription factor signatures accompanied by an Inflamed gene signature (SCLC-A, N, P, and I, respectively). SCLC-I experiences the greatest benefit from the addition of immunotherapy to chemotherapy, while the other subtypes each have distinct vulnerabilities, including to inhibitors of PARP, Aurora kinases, or BCL-2. Cisplatin treatment of SCLC-A patient-derived xenografts induces intratumoral shifts toward SCLC-I, supporting subtype switching as a mechanism of acquired platinum resistance. We propose that matching baseline tumor subtype to therapy, as well as manipulating subtype switching on therapy, may enhance depth and duration of response for SCLC patients. Gay et al. provide a classification for four subtypes of small cell lung cancer, each with unique molecular features and therapeutic vulnerabilities. An inflamed, mesenchymal subtype predicts benefit with the addition of immunotherapy to chemotherapy. Intratumoral switching between chemosensitive and chemoresistant subtypes accompanies therapeutic resistance.
- Published
- 2021
44. Floating Potential Boundary Condition in Smooth Domains in an Electroporation Context
- Author
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Sergio Corridore, Clair Poignard, Annabelle Collin, Modélisation Mathématique pour l'Oncologie (MONC), Institut de Mathématiques de Bordeaux (IMB), Université Bordeaux Segalen - Bordeaux 2-Université Sciences et Technologies - Bordeaux 1 (UB)-Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux (Bordeaux INP)-Centre National de la Recherche Scientifique (CNRS)-Université Bordeaux Segalen - Bordeaux 2-Université Sciences et Technologies - Bordeaux 1 (UB)-Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux (Bordeaux INP)-Centre National de la Recherche Scientifique (CNRS)-Institut Bergonié [Bordeaux], UNICANCER-UNICANCER-Inria Bordeaux - Sud-Ouest, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), This work was partially funded by the ITMO Cancer in the frame of the Plan Cancer 2014–2019 (project NUMEP PC201615)., Suzuki T, Poignard C, Chaplain M, and Quaranta V
- Subjects
Physics ,Asymptotic analysis ,Partial differential equation ,Thin Conductive Layer ,Asymptotic Analysis ,Numerical analysis ,Mathematical analysis ,Dirichlet to Neumann Operator ,Order of accuracy ,Context (language use) ,Electromagnetism ,Equipotential ,[MATH.MATH-AP]Mathematics [math]/Analysis of PDEs [math.AP] ,Boundary value problem ,Floating potential - Abstract
International audience; In electromagnetism, a conductor that is not connected to the ground is an equipo-tential whose value is implicitly determined by the constraint of the problem. It leads to a non-local constraints on the flux along the conductor interface, so-called floating potential problems. Unlike previous numerical study that tackle the floating potential problems with the help of advanced and complex numerical methods, we show how an appropriate use of Steklov-Poincaré operators enables to obtain the solution to this partial differential equations with a non local constraint as a linear (and well-designed) combination of N + 1 Dirichlet problems, N being the number of conductors not connected to a ground potential. In the case of thin highly conductive inclusion, we perform an asymptotic analysis to approach the electroquasistatic potential at any order of accuracy. In particular, we show that the so-called floating potential approaches the electroquasistatic potential with a first order accuracy. This enables us to characterize the configurations for which floating potential approximation has to be used to accurately solve the electroquasistatic problem.
- Published
- 2020
45. ACDC: Automated Cell Detection and Counting for time-lapse fluorescence microscopy
- Author
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Daniela Besozzi, Leonardo Rundo, Marco S. Nobile, Carlos F. Lopez, Darren R. Tyson, Vito Quaranta, Carmelo Militello, Riccardo Betta, Giancarlo Mauri, Andrea Tangherloni, Alexander L. R. Lubbock, Simone Spolaor, Paolo Cazzaniga, Rundo, L, Tangherloni, A, Tyson, D, Betta, R, Militello, C, Spolaor, S, Nobile, M, Besozzi, D, Lubbock, A, Quaranta, V, Mauri, G, Lopez, C, Cazzaniga, P, Information Systems IE&IS, Rundo, Leonardo [0000-0003-3341-5483], Tangherloni, Andrea [0000-0002-5856-4453], Tyson, Darren R [0000-0002-3272-4308], Militello, Carmelo [0000-0003-3177-9398], Spolaor, Simone [0000-0002-3383-367X], Nobile, Marco S [0000-0002-7692-7203], Besozzi, Daniela [0000-0001-5532-3059], Lubbock, Alexander L R [0000-0002-6950-8908], Quaranta, Vito [0000-0001-7491-8672], Mauri, Giancarlo [0000-0003-3520-4022], Lopez, Carlos F [0000-0003-3668-7468], Cazzaniga, Paolo [0000-0001-7780-0434], Apollo - University of Cambridge Repository, Tyson, Darren R. [0000-0002-3272-4308], Militello, Carmelo [0000-0003-2249-9538], Nobile, Marco S. [0000-0002-7692-7203], Lubbock, Alexander L. R. [0000-0002-6950-8908], and Lopez, Carlos F. [0000-0003-3668-7468]
- Subjects
Computer science ,02 engineering and technology ,lcsh:Technology ,fluorescence microscopy ,Fluorescence imaging ,lcsh:Chemistry ,0302 clinical medicine ,Computer cluster ,Microscopy ,0202 electrical engineering, electronic engineering, information engineering ,Fluorescence microscope ,General Materials Science ,Instrumentation ,lcsh:QH301-705.5 ,Cell counting ,Fluid Flow and Transfer Processes ,0303 health sciences ,Settore INF/01 - Informatica ,General Engineering ,Time-lapse microscopy ,INF/01 - INFORMATICA ,Fluorescence ,lcsh:QC1-999 ,Computer Science Applications ,Nuclei segmentation ,020201 artificial intelligence & image processing ,Bilateral filter ,Similarity (geometry) ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,Automated Cell Detection and Counting ,Article ,ING-INF/05 - SISTEMI DI ELABORAZIONE DELLE INFORMAZIONI ,03 medical and health sciences ,medicine ,Leverage (statistics) ,Bioimage informatic ,Bioimage informatics ,030304 developmental biology ,lcsh:T ,business.industry ,Process Chemistry and Technology ,ACDC ,Pattern recognition ,medicine.disease ,Visualization ,lcsh:Biology (General) ,lcsh:QD1-999 ,lcsh:TA1-2040 ,Artificial intelligence ,lcsh:Engineering (General). Civil engineering (General) ,business ,Bioimage Informatics ,030217 neurology & neurosurgery ,lcsh:Physics - Abstract
Advances in microscopy imaging technologies have enabled the visualization of live-cell dynamic processes using time-lapse microscopy imaging. However, modern methods exhibit several limitations related to the training phases and to time constraints, hindering their application in the laboratory practice. In this work, we present a novel method, named Automated Cell Detection and Counting (ACDC), designed for activity detection of fluorescent labeled cell nuclei in time-lapse microscopy. ACDC overcomes the limitations of the literature methods, by first applying bilateral filtering on the original image to smooth the input cell images while preserving edge sharpness, and then by exploiting the watershed transform and morphological filtering. Moreover, ACDC represents a feasible solution for the laboratory practice, as it can leverage multi-core architectures in computer clusters to efficiently handle large-scale imaging datasets. Indeed, our Parent-Workers implementation of ACDC allows to obtain up to a 3.7×, speed-up compared to the sequential counterpart. ACDC was tested on two distinct cell imaging datasets to assess its accuracy and effectiveness on images with different characteristics. We achieved an accurate cell-count and nuclei segmentation without relying on large-scale annotated datasets, a result confirmed by the average Dice Similarity Coefficients of 76.84 and 88.64 and the Pearson coefficients of 0.99 and 0.96, calculated against the manual cell counting, on the two tested datasets.
- Published
- 2020
46. Prove nazionali. I risultati del 46o anno di sperimentazione. Duro, i consigli varietali
- Author
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E. Gosparini, R. Mortaro, M. Zefelippo, R. Converso, M. Signor, G. Barbiani, A. Gianinetti, M. Baravelli, A. De Montis, R. Rosta, A. Massi, P. Mantovani, P. Bottazzi, L. Fabbrini, P. Viola, R. Ruggeri, F. Rossini, R. Mariotti, O. Basili, F. Quaranta, V. Mazzon, A. Belocchi, P. Cacciatori, P. Pesarini, A. Sestili, M. Fornara, V. Vecchiarelli, G. Mazzieri, C. Governatori, A. Petrini, D. Fuselli, R. Santilocchi, M. Bianchelli, G. Piani, M. Mori, I. Di Mola, L. Ottaiano, P. Codianni, A. Iannucci, S. Paone, S. A. Colecchia, M. Rinaldi, P. M. Romano, N. Pecchioni, M. Colonna, M. Ricci, L. Tedone, G. De Mastro, D. Schiavone, G. Preiti, G. Badagliacca, G. Pruneddu, R. Motzo, F. Giunta, V. Balmas, G. Carboni, M. Dettori, L. Mameli, A. S. Frenda, R. Ingraffia, B. Randazzo, U. Anastasi, S. Virgillito, L. Salafia, M. Palumbo, N. Virzì, F. Sciacca, and E. Gosparini, R. Mortaro, M. Zefelippo, R. Converso, M. Signor, G. Barbiani, A. Gianinetti, M. Baravelli, A. De Montis, R. Rosta, A. Massi, P. Mantovani, P. Bottazzi, L. Fabbrini, P. Viola, R. Ruggeri, F. Rossini, R. Mariotti, O. Basili, F. Quaranta, V. Mazzon, A. Belocchi, P. Cacciatori,P. Pesarini, A. Sestili, M. Fornara, V. Vecchiarelli, G. Mazzieri, C. Governatori, A. Petrini, D. Fuselli, R. Santilocchi, M. Bianchelli, G. Piani, M. Mori, I. Di Mola, L. Ottaiano, P. Codianni, A. Iannucci, S. Paone, S.A. Colecchia,M. Rinaldi, P. M. Romano, N. Pecchioni, F. Quaranta, M. Colonna, M. Ricci, M. Fornara, L. Tedone, G. De Mastro, D. Schiavone, G. Preiti, G. Badagliacca, G. Pruneddu, R. Motzo, F. Giunta, V. Balmas, G. Carboni, M. Dettori, L. Mameli, A.S. Frenda, R. Ingraffia, B. Randazzo, U. Anastasi, S. Virgillito, L. Salafia, M. Palumbo, N. Virzì, F. Sciacca
- Subjects
Frumento duro ,Valutazione varietale ,Settore AGR/02 - Agronomia E Coltivazioni Erbacee - Abstract
In 31 campi dei principali areali di coltivazione italiani sono stati valutati 38 genotipi, di cui 12 al primo anno di prova. La cultivar mediamente più produttiva è ri sultata LG anubis, al primo anno, con indice 108 e medie superate in 25 prove su 31 totali (81% dei campi), caratterizzata da peso ettolitrico e tenore proteico leggermente inferiori alle medie (-0,1 e -0,4 punti, rispettivamente).
- Published
- 2019
47. WIND TUNNEL MEASUREMENTS OF PRESSURE FLUCTUATIONS AND STRUCTURAL RESPONSE INDUCED BY THE TURBULENT BOUNDARY LAYER AT HIGH MACH NUMBER, PART 2: COMPARISONS BETWEEN EXPERIMENTAL AND NUMERICAL DATA FOR THE STRUCTURAL RESPONSE
- Author
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Sergio De Rosa, Francesco Franco, Elena Ciappi, Francesca Magionesi, Vincenzo Quaranta, Pasquale Vitiello, Massimiliano Di Giulio, DE ROSA, Sergio, Franco, Francesco, E., Ciappi, F., Magionesi, V., Quaranta, P., Vitiello, M., Di Giulio, Ciappi, E., Magionesi, F., Quaranta, V., Vitiello, P., and Di Giulio, M.
- Abstract
Measurements of the acceleration response of typical homogeneous and composite aeronautical panels with different lay-up to turbulent boundary layer excitation have been performed in a transonic wind tunnel for Mach number values ranging between 0.4 and 0.9. Artificial boundary layers of different thickness have been generated using the setup described in PART 1. Aim of this experimental campaign was the understanding of the influence of different materials and geometrical properties on the response of aeronautical panels to random convective loads as a starting point for a following acoustical optimization. Numerical simulations of the panel response have been performed by FEM and in house codes using, as a forcing function, the modeled pressure field and have been validated by comparisons with measured acceleration data. The numerical determination of the structural response is also fundamental to understand how and when the theoretical models developed for the representation of wall pressure fluctuation spectra can be considered predictive thus, independent on the particular flow conditions.
- Published
- 2012
48. Procedimento cautelare
- Author
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SANDULLI, Maria Alessandra, A. Quaranta – V. Lopilato, and Sandulli, Maria Alessandra
- Published
- 2011
49. Localization and possible role of two different alpha v beta 3 integrin conformations in resting and resorbing osteoclasts
- Author
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Alberta Zallone, Maria Grano, Antonello Villa, Silvia Colucci, Gianluigi Giannelli, Vito Quaranta, Roberta Faccio, Faccio, R, Grano, M, Colucci, S, Villa, A, Giannelli, G, Quaranta, V, and Zallone, A
- Subjects
musculoskeletal diseases ,Macrophage colony-stimulating factor ,Alpha-v beta-3 ,Protein Conformation ,Integrin ,Osteoclasts ,Fluorescent Antibody Technique ,Biology ,Bone and Bones ,Bone resorption ,Phosphatidylinositol 3-Kinases ,chemistry.chemical_compound ,Cell surface receptor ,Osteoclast ,Cell Movement ,medicine ,Tumor Cells, Cultured ,Humans ,Protein Isoforms ,1-Phosphatidylinositol 3-Kinase ,Enzyme Inhibitor ,Enzyme Inhibitors ,Bone Resorption ,Phosphoinositide-3 Kinase Inhibitors ,Integrin alphaVbeta3 ,Hepatocyte Growth Factor ,Macrophage Colony-Stimulating Factor ,Cell Membrane ,Protein Isoform ,Antibodies, Monoclonal ,Cell Biology ,Cell biology ,Resorption ,medicine.anatomical_structure ,Biochemistry ,chemistry ,biology.protein ,Bone Remodeling ,Human ,Bone and Bone - Abstract
Integrins are membrane receptors that mediate interactions between cells and the extracellular matrix. We recently showed that the osteoclast integrin alpha(v)beta(3) exists in two different conformations, so-called 'basal' and 'activated', with each exhibiting a distinct function. In this study we demonstrate that, in non-resorbing osteoclasts, the 'activated' form of alpha(v)beta(3) accumulates in the motile areas of the plasma membrane. During bone resorption this conformation is prevalent in the ruffled membrane, whereas the 'basal' form of alpha(v)beta(3) is also present in the sealing zone. Moreover, hepatocyte growth factor (HGF) and macrophage colony stimulating factor (M-CSF), two molecules involved in osteoclastogenesis and osteoclast survival, modulate alpha(v)beta(3) conformation in vitro. Preincubation with HGF or M-CSF induces a shift of conformation of alpha(v)beta(3) in primary human osteoclasts (OCs) and in the osteoclast-like cell line (GCT 23). Activated integrin promotes osteoclast migration to the alpha(v)beta(3) ligand osteopontin and enhances bone resorption. Thus, HGF and M-CSF modulate the alpha(v)beta(3) conformational states required for osteoclast polarization and resorption. The capacity of growth factors to alter the affinity of alpha(v)beta(3) toward its ligands offers a potential explanation for the diverse responses of osteoclasts to the same ligand.
- Published
- 2002
50. Laminin receptors in the retina: sequence analysis of the chick integrin alpha 6 subunit. Evidence for transcriptional and posttranslational regulation
- Author
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Louis F. Reichardt, I de Curtis, R N Tamura, Vito Quaranta, DE CURTIS, Ivanmatteo, Quaranta, V, Tamura, Rn, and Reichardt, Lf
- Subjects
Retinal Ganglion Cells ,Integrins ,Transcription, Genetic ,Integrin ,Molecular Sequence Data ,Restriction Mapping ,Sequence Homology ,Chick Embryo ,Retinal ganglion ,CD49c ,Medical and Health Sciences ,Retina ,Receptors, Laminin ,Genetic ,Laminin ,Immunologic ,Sequence Homology, Nucleic Acid ,Receptors ,medicine ,Animals ,Humans ,Amino Acid Sequence ,Cloning, Molecular ,Receptors, Immunologic ,Regulation of gene expression ,biology ,Nucleic Acid ,Base Sequence ,Molecular ,Cell Biology ,DNA ,Articles ,Biological Sciences ,Molecular biology ,Precipitin Tests ,medicine.anatomical_structure ,Integrin alpha M ,Gene Expression Regulation ,Protein Biosynthesis ,biology.protein ,Integrin, beta 6 ,Transcription ,Cloning ,Developmental Biology - Abstract
The integrin alpha 6 beta 1 is a prominent laminin receptor used by many cell types. In the present work, we isolate clones and determine the primary sequence of the chick integrin alpha 6 subunit. We show that alpha 6 beta 1 is a prominent integrin expressed by cells in the developing chick retina. Between embryonic days 6 and 12, both retinal ganglion cells and other retinal neurons lose selected integrin functions, including the ability to attach and extend neurites on laminin. In retinal ganglion cells, we show that this is correlated with a dramatic decrease in alpha 6 mRNA and protein, suggesting that changes in gene expression account for the developmental regulation of the interactions of these neurons with laminin. In other retinal neurons the expression of alpha 6 mRNA and protein remains high while function is lost, suggesting that the function of the alpha 6 beta 1 heterodimer in these cells is regulated by posttranslational mechanisms.
- Published
- 1991
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