1. Effects of berberine hydrochloride on methamphetamine-induced anxiety behaviors and relapse in rats
- Author
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Rezaeian, Leila, Kalalian-Moghaddam, Hamid, Mohseni, Fahimeh, Khaksari, Mehdi, and Rafaiee, Raheleh
- Subjects
relapse ,berberine ,neuronal cell death ,lcsh:R ,lcsh:Medicine ,Original Article ,neuroprotection ,anxiety ,methamphetamine - Abstract
Objective(s): This research aimed at evaluating the effect of berberine hydrochloride on anxiety-related behaviors induced by methamphetamine (METH) in rats, assessing relapse and neuroprotective effects. Materials and Methods: 27 male Wistar rats were randomly assigned into groups of Control, METH-withdrawal (METH addiction and subsequent withdrawal), and METH addiction with berberine hydrochloride oral treatment (100 mg/kg/per day) during the three weeks of withdrawal. Two groups received inhaled METH self-administration for two weeks (up to 10 mg/kg). The elevated plus maze (EPM) test and open field test (OFT) were carried out one day after the last berberine treatment and relapse was assessed by conditional place preference (CPP) test. TUNEL assay and immunofluorescence staining for NF-κB, TLR4, Sirt1, and α-actin expression in the hippocampus were tested. Results: After 3 weeks withdrawal, berberine hydrochloride decreased locomotor activity and reduced anxiety-related behaviors in comparison with the METH-withdrawal group (p
- Published
- 2020