123 results on '"S, Hammarström"'
Search Results
2. Redefined Nomenclature for Members of the Carcinoembryonic Antigen Family
- Author
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N, Beauchemin, P, Draber, G, Dveksler, P, Gold, S, Gray-Owen, F, Grunert, S, Hammarström, K V, Holmes, A, Karlsson, M, Kuroki, S H, Lin, L, Lucka, S M, Najjar, M, Neumaier, B, Obrink, J E, Shively, K M, Skubitz, C P, Stanners, P, Thomas, J A, Thompson, M, Virji, S, von Kleist, C, Wagener, S, Watt, and W, Zimmermann
- Subjects
Cell Biology ,Computational biology ,Pregnancy Proteins ,Biology ,Antigens, Differentiation ,Carcinoembryonic Antigen ,Rats ,Alternative Splicing ,Mice ,Carcinoembryonic antigen ,Genes ,Antigens, CD ,Terminology as Topic ,Biomarkers, Tumor ,biology.protein ,Animals ,Humans ,Female ,Cell Adhesion Molecules ,Nomenclature ,Pseudogenes - Published
- 1999
3. Gamma delta T cells of human early pregnancy decidua: evidence for local proliferation, phenotypic heterogeneity, and extrathymic differentiation
- Author
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L Mincheva-Nilsson, M Kling, S Hammarström, O Nagaeva, K G Sundqvist, M L Hammarström, and V Baranov
- Subjects
Immunology ,Immunology and Allergy - Abstract
The uterine mucosa in pregnancy, the decidua, allows placenta formation and survival of the fetus despite the fact that it is semiallogeneic. Decidua contains large numbers of lymphocytes, of which CD56+ cells dominate, followed by T cells expressing either alpha beta or gamma delta TCR. We have investigated the developmental relationship between the CD56- and TCR gamma delta-expressing cells in early pregnancy decidua using dual labeling immunoelectron microscopy, immunoflow cytometry, and cell fractionation. Lymphocyte subpopulations were, in addition, analyzed for expression of the cytokine receptor for IL-7 and c-kit and for mRNA expression of recombinase-activating genes 1 and 2. Four different cell populations could be distinguished: CD56+bright, CD56+dim/TCR gamma delta+low, CD56+dim/TCR gamma delta+high, and TCR gamma delta+low. Recombinase-activating genes 1 and 2 were expressed in the CD56+bright cells and to a limited degree in CD56+dim/TCR gamma delta+low cells. c-kit was preferentially expressed on the CD56+bright cells, while IL-7R was preferentially expressed on CD56+dim/TCR gamma delta+low and CD56+dim/TCR gamma delta+high cells. The CD56+dim TCR gamma delta+low and CD56+dim/TCR gamma delta+high cells displayed the characteristic morphology of large granular lymphocytes, while single positive TCR gamma delta+low cells were usually smaller and did not contain cytoplasmic granules. The gamma delta 1 gene segment was almost exclusively used in the TCR. Gamma delta T cells in mitosis were seen. We suggest that human early pregnancy decidua is a transient site for extrathymic maturation and that the progenitors of TCR gamma delta+ cells are bone marrow-derived immature cells expressing the CD56 (neural cell adhesion molecule) homing receptor.
- Published
- 1997
4. Phenotype and Cytokine Profile of Intraepithelial Lymphocytes in Human Small and Large Intestine
- Author
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S. Hammarström, Kalle Söderström, C R. Messling, V. BARANOVf, M.-L. Hammarström, C. Lundqvistf, and L. Athltn
- Subjects
T-Lymphocytes ,General Neuroscience ,Cytokine profile ,Gene Expression ,Epithelial Cells ,Biology ,Flow Cytometry ,Phenotype ,Epithelium ,General Biochemistry, Genetics and Molecular Biology ,Immunophenotyping ,medicine.anatomical_structure ,History and Philosophy of Science ,Intestine, Small ,Immunology ,medicine ,Cytokines ,Humans ,Intraepithelial lymphocyte ,Large intestine ,Intestine, Large ,RNA, Messenger - Published
- 1995
5. Cytokine profile and ultrastructure of intraepithelial gamma delta T cells in chronically inflamed human gingiva suggest a cytotoxic effector function
- Author
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C Lundqvist, V Baranov, S Teglund, S Hammarström, and M L Hammarström
- Subjects
Immunology ,Immunology and Allergy - Abstract
We have shown that gamma delta T cells in human gingiva have an intraepithelial location and, that in the chronic inflammatory disease periodontitis, the expression of CD45RO and CD8 or CD4 is induced on gamma delta T cells. To study the role of gamma delta T cells in local antibacterial responses, we determined the cytokine profiles of isolated human gingival cells. Different T cell subpopulations, isolated by positive selection with mAb-coated magnetic beads and macrophages, as well as epithelial cells, were analyzed for expression of mRNA for 15 cytokines by reverse transcriptase-PCR. The ultrastructure of gingival gamma delta T cells was also studied. The gamma delta T cells expressed mRNA for IFN-gamma, TNF-alpha, TGF-beta 1, and IL-6. Expression of IFN-gamma was a consequence of inflammation. CD4+ gamma delta T cells expressed IFN-gamma only, whereas CD8+ gamma delta T cells expressed all four cytokines. CD8+ cells expressing IFN-gamma, TNF-alpha, and IL-6 in combination suggest a cytotoxic effector function. Gingival gamma delta T cells contained cytoplasmic electron-dense membrane-bound granules and multivesicular bodies that are ultrastructural characteristics of cytotoxic cells. Epithelial cells from inflamed gingiva expressed HLA-DR, CD1a, CD1c, and heat shock protein 60 on the cell surface. They also expressed mRNA for IL-1 beta, IL-6, IL-8, TNF-alpha, and TGF-beta 1. Thus, epithelial cells may function as accessory cells in immune activation and, at the same time, be target cells for CD8+ gamma delta T cells reactive with CD1 Ag or heat shock protein. These results suggest that gamma delta T cells constitute a first line of defense in gingiva, preventing entrance of pathogens by cytotoxicity against infected and stressed epithelial cells, and by control of epithelial cell growth through secretion of regulatory cytokines.
- Published
- 1994
6. Workshop: Immunotoxicology and in vitro possibilities
- Author
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J. Descotes, A. Sundwall, J. Dean, Kimber L. White, J. Vos, M. Lorentz, Ian Kimber, S. Hammarström, Lena Odland, Erik Walum, H.O. Sjögren, Michael Balls, B. Andersson, Michael I. Luster, P. Hultman, V. Stejskal, B. Veronesi, and P. Moldéus
- Subjects
business.industry ,Immunology ,Medicine ,General Medicine ,Immunotoxicology ,Toxicology ,business - Published
- 2010
7. Human decidual leukocytes from early pregnancy contain high numbers of gamma delta+ cells and show selective down-regulation of alloreactivity
- Author
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L Mincheva-Nilsson, S Hammarström, and M L Hammarström
- Subjects
Immunology ,Immunology and Allergy - Abstract
The mononuclear lymphoid cell population in human pregnant uterus mucosa, decidua, from early normal pregnancies was studied phenotypically and functionally. The phenotype was determined in situ by immunohistochemistry, and in isolated decidual mononuclear cell preparations by immunofluorescence and flow cytometry. A mild isolation procedure of gentle mechanical disruption followed by density gradient centrifugation was used. Leukocytes comprised a large part of the decidual tissue. They were present in aggregates mainly situated adjacent to the glandular epithelium. In addition, individual leukocytes were present intraepithelially, as well as scattered between the stromal cells and around vessels and lacunes. Four lymphocyte populations of approximately the same size were identified: TCR gamma delta+/CD56+ cells, TCR gamma delta+/CD56- cells, TCR gamma delta-/CD56+ cells, and TCR alpha beta+/CD8+ cells. TCR gamma delta- expressing cells comprised about 60% of the T cells. They were CD4-/CD8-, and about half of the TCR gamma delta+ cells expressed the memory/activation marker CD45RO. The Kp 43 Ag, earlier described on activated CD56+ and TCR gamma delta+ cells in peripheral blood, was essentially only expressed on the TCR gamma delta-/CD56+ cell population in decidua. At least 50% of the TCR alpha beta+ cells were CD8+. The function(s) of either one of these populations might be to prevent immunologic reactions against the fetus, to protect the uterus from unwanted extensive invasion of trophoblasts, or to protect the uteroplacental unit from infection. Decidual T cells did not respond to stimulation by alloantigens or mitogenic anti-CD3 mAb but responded to the same extent as PBMC to mitogenic lectins. The surface density of the TCR/CD3 complex was low on freshly isolated decidual lymphocytes, but could be up-regulated upon stimulation with PMA/Ionomycin. Local selective down-regulation of surface expression of the TCR/CD3 complex and of activation involving this complex might be one of the mechanisms by which a maternal immunologic reaction against the semiallogeneic fetus is prevented.
- Published
- 1992
8. Protein epitopes in carcinoembryonic antigen. Report of the ISOBM TD8 workshop
- Author
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J, Bjerner, Y, Lebedin, L, Bellanger, M, Kuroki, J E, Shively, T, Varaas, K, Nustad, S, Hammarström, and O P, Børmer
- Subjects
Antibody Affinity ,Radioimmunoassay ,Antibodies, Monoclonal ,Recombinant Proteins ,Carcinoembryonic Antigen ,Education ,Protein Structure, Tertiary ,Epitopes ,Kinetics ,Mice ,Animals ,Humans ,Epitope Mapping ,Protein Binding - Abstract
To characterize antigenic sites in carcinoembryonic antigen (CEA) further and to investigate whether there are differences between colon tumor CEA and meconium CEA (NCA-2) that can be detected by anti-CEA monoclonal antibodies (MAb), 19 new anti-CEA MAb were analyzed with respect to specificity, epitope reactivity and affinity. Their reactivities were compared with 10 anti-CEA MAb with known CEA-domain binding specificity that have previously been classified into five nonoverlapping epitope groups, GOLD 1-5. Cross-inhibition assays with antigen-coated microtiter plates and immunoradiometric assays were performed in almost all combinations of MAbs, using conventionally purified CEA (domain structure: N-A1B1-A2B2-A3B3-C) from liver metastasis of colorectal carcinomas, recombinant CEA, meconium CEA (NCA-2), truncated forms of CEA and NCA (CEACAM6) as the antigens. The affinity of the MAbs for CEA was also determined. The new MAbs were generally of high affinity and suitable for immunoassays. Three new MAbs were assigned to GOLD epitope group 5 (N-domain binding), 3 MAbs to group 4 (A1B1 domain), 1 to group 3 (A3B3 domain), 3 to group 2 (A2B2 domain) and 3 to group 1 (also the A3B3 domain). Three MAbs formed a separate group related to group 4, they were classified as GOLD 4' (A1B1 domain binding). The remaining 3 MAbs appear to represent new subspecificities with some relationship to GOLD groups 1, 2 or 4, respectively. Five MAbs, all belonging to epitope group 1 and 3, reacted strongly with tumor CEA but only weakly or not at all with meconium CEA, demonstrating that the two products of the CEA gene differ from each other, probably due to different posttranslational modifications.
- Published
- 2002
9. Pregnancy-specific glycoprotein (PSG) in baboon (Papio hamadryas): family size, domain structure, and prediction of a functional region in primate PSGs
- Author
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G Q, Zhou and S, Hammarström
- Subjects
DNA, Complementary ,Placenta ,Gene Expression ,Pregnancy Proteins ,Polymerase Chain Reaction ,Recombinant Proteins ,Rats ,Mice ,Pregnancy ,Animals ,Humans ,Female ,Sequence Alignment ,Phylogeny ,Glycoproteins ,Papio - Abstract
Pregnancy-specific glycoprotein (PSG) constitutes a major component of serum of pregnant women and appears to be essential for a successful pregnancy. Its function is, however, still unknown. Because of the evolutionary divergence between human and rodent PSG, functional studies may require a primate animal model. We have characterized PSG transcripts in a baboon placenta cDNA library and analyzed baboon genomic DNA. The main PSG isoform had the domain structure N-A1-B2-C similar to the human type IIa isoform. The type I isoform (N-A1-A2-B2-C) was also expressed. Fifteen similar PSG genes were identified of which at least nine were simultaneously expressed in third trimester baboon placenta. Thus, the baboon PSG family was as complex as that of humans. Recombinant baboon PSG (isoform IIa) had a molecular weight of 38 kDa and reacted with antibodies against human PSG. Comparative analysis of 43 N-domain amino acid sequences of PSG from four species and nine primate carcinoembryonic antigen subgroup N domain sequences identified a number of residues in the GFCC'C" ss-sheet and FG loop that are probable candidates for PSG binding to its putative ligand.
- Published
- 2001
10. Evolution of the carcinoembryonic antigen family. structures of CGM9, CGM11 and pregnancy-specific glycoprotein promoters
- Author
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L, Frängsmyr, A, Israelsson, S, Teglund, T, Matsunaga, and S, Hammarström
- Subjects
Genomic Library ,Base Sequence ,Molecular Sequence Data ,Pregnancy Proteins ,Carcinoembryonic Antigen ,Rats ,Evolution, Molecular ,Mice ,Fetus ,Liver ,Gene Duplication ,Multigene Family ,Sequence Homology, Nucleic Acid ,Consensus Sequence ,Animals ,Humans ,Amino Acid Sequence ,Promoter Regions, Genetic ,Sequence Alignment ,Phylogeny ,Pseudogenes ,Glycoproteins - Abstract
Earlier studies have demonstrated that the genes of the human carcinoembryonic antigen (CEA) family can be divided into three subgroups, the CEA subgroup (n = 12), the pregnancy-specific glycoprotein (PSG) subgroup (n = 11), and a third subgroup (n = 6). To further characterize the CEA gene family, we have determined the genomic structures of CGM9 and CGM11, analyzed the promoter regions of all eleven PSGs, studied the CGM15-PSG13 intergenic region and the evolutionary relationships beween the CEA family genes. CGM9, a typical CEA subgroup member, was a pseudogene with the exon structure [5'UTR-L-L/N-TM-Cyt-3'UTR]. CGM11 contained a mixture of exons derived from CEA and PSG subgroup genes. The formula of the CGM11 pseudogene was [5'UTR-L-L/N-C-3'UTR]. Thus both genes lacked the IgC2-like domains typically found in CEA subfamily members. The upstream promoter regions of all eleven PSGs were characterized. All PSG promoters lacked the classical TATA and CCAAT elements, but had putative PEA3 box(es), CACCC box(es), a RARE box, and poly (dG-dT) repeats of different lengths. Five PSGs also had an SP1 site. The complete 10-kb intergenic region between CGM15 and PSG13 was sequenced. Clusters of different types of repetitive sequences were seen. The time of divergence of the CEA and PSG subfamilies was estimated to be 107.7 +/- 17.1 million years, or at about the time of human-rodent divergence. Models for the evolution of CEA and PSG and the third family subgroup genes are proposed.
- Published
- 2000
11. Subunits and cellular occurrence of the 12(S)-HETE binding complex
- Author
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H, Herbertsson, T, Kühme, and S, Hammarström
- Subjects
Binding Sites ,Macromolecular Substances ,Precipitin Tests ,Cell Line ,Molecular Weight ,Mice ,Adenosine Triphosphate ,Cytosol ,Receptors, Eicosanoid ,Animals ,Humans ,HSP70 Heat-Shock Proteins ,12-Hydroxy-5,8,10,14-eicosatetraenoic Acid ,Protein Structure, Quaternary - Published
- 2000
12. Apoptosis and p53 gene expression in male reproductive tissues of cadmium exposed rats
- Author
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G, Xu, G, Zhou, T, Jin, T, Zhou, S, Hammarström, A, Bergh, and G, Nordberg
- Subjects
Male ,Testis ,Prostate ,Animals ,Gene Expression ,Reproducibility of Results ,Apoptosis ,Metallothionein ,RNA, Messenger ,Rats, Wistar ,Genes, p53 ,Cadmium ,Rats - Abstract
Reverse transcription (RT) PCR technique was used to investigate the mechanism of apoptosis induced by Cd and the change of its related genes in testes and prostate of rats. Adult male rats were given a single (s.c.) injection of CdCl2 0, 2.5, 5.0, 10 mumol/kg. 48 h and 72 h after administration of Cd, animals were sacrificed. The results indicated that Cd can induce apoptosis in testes via p53-independent pathway. No apoptosis occurred in prostate in any of the Cd-exposed groups. There was a clearly negative relationship in testes between p53 gene expression and Cd exposure and this dose-response relationship was observed both at 48 h and 72 h. There was a very small increase of this gene expression in the dorsolateral lobe of the prostate in Cd exposed groups. The other apoptosis related gene, bcl-x, was not detectable in either control or Cd-exposed group in testes and dorsal prostate. Although the MT-I gene was expressed in testes or dorsal prostate both in control and exposed groups, no overexpression of MT-I gene was found after administration of Cd. The expression of MT-I in the ventral prostate was not detected in the control group, but a weak expression was found after Cd exposure. Since p53 is a tumor suppressor gene which can inhibit tumorigenesis, the consequence of a Cd-induced decrease of p53 in testes may have a relation to the known risk of Cd tumorigenesis in this tissue.
- Published
- 1999
13. A 12(S)-HETE receptor in Lewis lung carcinoma cells
- Author
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H, Herbertsson, T, Kühme, and S, Hammarström
- Subjects
Carcinoma, Lewis Lung ,Binding Sites ,Rats, Inbred Lew ,Receptors, Eicosanoid ,Tumor Cells, Cultured ,Animals ,Rats - Published
- 1999
14. Activated human gamma delta T lymphocytes express functional lactoferrin receptors
- Author
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L, Mincheva-Nilsson, S, Hammarström, and M L, Hammarström
- Subjects
Kinetics ,Lactoferrin ,T-Lymphocytes ,Receptors, Transferrin ,Decidua ,Leukocytes, Mononuclear ,Humans ,Female ,Receptors, Antigen, T-Cell, gamma-delta ,Receptors, Cell Surface ,Lymphocyte Activation ,Cell Division ,Lymphocyte Subsets - Abstract
Lactoferrin (Lf), an iron-binding protein in milk, mucosal secretions and neutrophil granules has bactericidal properties and is a source of iron for breast-fed infants. In this paper the authors show that most in vivo activated lymphocytes, i.e. freshly isolated lymphocytes from first trimester human decidua, and most in vitro activated human blood lymphocytes, express lactoferrin receptors (Lf-R), while unstimulated blood lymphocytes do not. All major lymphocyte subsets, i.e. alpha beta T cells, gamma delta T cells, CD8+ T cells, CD4+ T cells, B cells and NK cells, express Lf-R after activation. The proportion of Lf-R expressing activated gamma delta T cells is significantly larger than that of activated alpha beta T cells. Lf-R and transferrin receptors (Tr-R/CD71) show the same kinetics of appearance on activated blood lymphocytes and are, to a large extent, expressed on the same cells. However, 35% of decidual lymphocytes and 15% of activated blood lymphocytes express Lf-R only. Addition of Lf to cultures containing an optimal concentration of Tr augments the proliferative response to polyclonal T cell activators and alloantigens, suggesting that presently used standard culture conditions for in vitro activation are suboptimal in particular for gamma delta T cells. Lf-R on decidual lymphocytes contain bound Lf, which probably is produced locally. The results suggest that Lf is a growth-supporting factor, especially important in local immune responses in the mucosa.
- Published
- 1998
15. Gamma delta T cells of human early pregnancy decidua: evidence for local proliferation, phenotypic heterogeneity, and extrathymic differentiation
- Author
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L, Mincheva-Nilsson, M, Kling, S, Hammarström, O, Nagaeva, K G, Sundqvist, M L, Hammarström, and V, Baranov
- Subjects
Genes, RAG-1 ,Cell Separation ,Thymus Gland ,Lymphocyte Activation ,Immunophenotyping ,Recombinases ,Antigens, CD ,Pregnancy ,T-Lymphocyte Subsets ,Decidua ,Humans ,Homeodomain Proteins ,Receptors, Interleukin-7 ,Integrases ,Interleukin-7 ,Nuclear Proteins ,Cell Differentiation ,Receptors, Antigen, T-Cell, gamma-delta ,Receptors, Interleukin ,Hematopoietic Stem Cells ,CD56 Antigen ,DNA-Binding Proteins ,Pregnancy Trimester, First ,Proto-Oncogene Proteins c-kit ,DNA Nucleotidyltransferases ,Leukocytes, Mononuclear ,Female - Abstract
The uterine mucosa in pregnancy, the decidua, allows placenta formation and survival of the fetus despite the fact that it is semiallogeneic. Decidua contains large numbers of lymphocytes, of which CD56+ cells dominate, followed by T cells expressing either alpha beta or gamma delta TCR. We have investigated the developmental relationship between the CD56- and TCR gamma delta-expressing cells in early pregnancy decidua using dual labeling immunoelectron microscopy, immunoflow cytometry, and cell fractionation. Lymphocyte subpopulations were, in addition, analyzed for expression of the cytokine receptor for IL-7 and c-kit and for mRNA expression of recombinase-activating genes 1 and 2. Four different cell populations could be distinguished: CD56+bright, CD56+dim/TCR gamma delta+low, CD56+dim/TCR gamma delta+high, and TCR gamma delta+low. Recombinase-activating genes 1 and 2 were expressed in the CD56+bright cells and to a limited degree in CD56+dim/TCR gamma delta+low cells. c-kit was preferentially expressed on the CD56+bright cells, while IL-7R was preferentially expressed on CD56+dim/TCR gamma delta+low and CD56+dim/TCR gamma delta+high cells. The CD56+dim TCR gamma delta+low and CD56+dim/TCR gamma delta+high cells displayed the characteristic morphology of large granular lymphocytes, while single positive TCR gamma delta+low cells were usually smaller and did not contain cytoplasmic granules. The gamma delta 1 gene segment was almost exclusively used in the TCR. Gamma delta T cells in mitosis were seen. We suggest that human early pregnancy decidua is a transient site for extrathymic maturation and that the progenitors of TCR gamma delta+ cells are bone marrow-derived immature cells expressing the CD56 (neural cell adhesion molecule) homing receptor.
- Published
- 1997
16. Hsp70: a subunit of the cytosolic 12(S)-HETE binding complex
- Author
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T, Kühme, H, Herbertsson, and S, Hammarström
- Subjects
Mice ,Adenosine Triphosphate ,Binding Sites ,Cytosol ,Lung Neoplasms ,Cell-Free System ,Macromolecular Substances ,Lipoxygenase ,Tumor Cells, Cultured ,Animals ,HSP70 Heat-Shock Proteins ,12-Hydroxy-5,8,10,14-eicosatetraenoic Acid ,Substrate Specificity - Published
- 1997
17. Cytosolic 12(S)-hydroxy-5,8,10,14-eicosatetraenoic acid binding sites in carcinoma cells
- Author
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H, Herbertsson and S, Hammarström
- Subjects
Cell Nucleus ,Binding Sites ,Lung Neoplasms ,Cell Membrane ,Cell Fractionation ,Mitochondria ,Mice ,Cytosol ,Receptors, Eicosanoid ,Centrifugation, Density Gradient ,Chromatography, Gel ,Tumor Cells, Cultured ,Animals ,12-Hydroxy-5,8,10,14-eicosatetraenoic Acid - Abstract
12(S)-HETE stimulates gene and cell surface expression of the integrin GPIIb/IIIa in carcinoma cells. The cells have high affinity binding sites for 12(S)-HETE. Analyses of the subcellular distribution and size of these sites showed that cytosol was the predominant location and that the apparent molecular weight was close to 670,000. Besides cytosol, mitochondria, nuclei, and plasma membranes also contained 12(S)-HETE binding sites. The mainly cytosolic location of the binding sites is different from that of other eicosanoid receptors which are G-protein coupled plasma membrane proteins of the rhodopsin gene family.
- Published
- 1997
18. Cell- and region-specific expression of biliary glycoprotein and its messenger RNA in normal human colonic mucosa
- Author
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L, Frängsmyr, V, Baranov, F, Prall, M M, Yeung, C, Wagener, and S, Hammarström
- Subjects
Adult ,Colon ,Antibodies, Monoclonal ,RNA Probes ,Immunohistochemistry ,Sensitivity and Specificity ,Microscopy, Electron ,Antibody Specificity ,Antigens, CD ,Colonic Neoplasms ,Humans ,RNA, Messenger ,Intestinal Mucosa ,Cell Adhesion Molecules ,In Situ Hybridization ,Glycoproteins - Abstract
The localization of biliary glycoprotein (BGP) and its mRNA in normal colonic mucosa was studied by immunohistochemistry and in situ hybridization. BGP mRNA was confined to columnar epithelial cells and expressed abundantly in the superficial mature cells and at low levels in differentiating cells in the upper crypts. Epithelial expression of BGP coincided with that of BGP mRNA. Ultrastructurally, BGP was localized to microfilaments of the fuzzy coat of the columnar cells at the luminal surface and the upper crypts. Additionally, BGP was found in cryptal caveolated cells. The results are consistent with primary transcriptional regulation of BGP production and suggest that BGP synthesis is controlled by the degree of cytodifferentiation. The fuzzy-coat localization of BGP implies a role in nonspecific defense mechanisms against pathogens.
- Published
- 1995
19. Functional lactoferrin receptors on activated human lymphocytes
- Author
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M L, Hammarström, L, Mincheva-Nilsson, and S, Hammarström
- Subjects
Adult ,Transferrin ,Antibodies, Monoclonal ,Receptors, Antigen, T-Cell, gamma-delta ,Receptors, Cell Surface ,Flow Cytometry ,Lymphocyte Activation ,Monocytes ,Lactoferrin ,Pregnancy Trimester, First ,Pregnancy ,T-Lymphocyte Subsets ,Decidua ,Humans ,Female ,Mitogens - Published
- 1995
20. Intraepithelial lymphocytes expressing TCR gamma delta in human gingival tissues
- Author
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C, Lundqvist, S, Hammarström, and M L, Hammarström
- Subjects
Adult ,Adolescent ,Gingiva ,Epithelial Cells ,Receptors, Antigen, T-Cell, gamma-delta ,Middle Aged ,Immunohistochemistry ,Epithelium ,Phenotype ,Aggressive Periodontitis ,T-Lymphocyte Subsets ,Cytokines ,Humans ,RNA, Messenger ,Periodontitis ,Aged - Published
- 1995
21. Cytokine profile and ultrastructure of intraepithelial gamma delta T cells in chronically inflamed human gingiva suggest a cytotoxic effector function
- Author
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C, Lundqvist, V, Baranov, S, Teglund, S, Hammarström, and M L, Hammarström
- Subjects
Macrophages ,Gingiva ,Gene Expression ,Epithelial Cells ,Receptors, Antigen, T-Cell, gamma-delta ,In Vitro Techniques ,Gingivitis ,Epithelium ,Immunophenotyping ,Interferon-gamma ,Microscopy, Electron ,T-Lymphocyte Subsets ,Chronic Disease ,Cytokines ,Humans ,RNA, Messenger - Abstract
We have shown that gamma delta T cells in human gingiva have an intraepithelial location and, that in the chronic inflammatory disease periodontitis, the expression of CD45RO and CD8 or CD4 is induced on gamma delta T cells. To study the role of gamma delta T cells in local antibacterial responses, we determined the cytokine profiles of isolated human gingival cells. Different T cell subpopulations, isolated by positive selection with mAb-coated magnetic beads and macrophages, as well as epithelial cells, were analyzed for expression of mRNA for 15 cytokines by reverse transcriptase-PCR. The ultrastructure of gingival gamma delta T cells was also studied. The gamma delta T cells expressed mRNA for IFN-gamma, TNF-alpha, TGF-beta 1, and IL-6. Expression of IFN-gamma was a consequence of inflammation. CD4+ gamma delta T cells expressed IFN-gamma only, whereas CD8+ gamma delta T cells expressed all four cytokines. CD8+ cells expressing IFN-gamma, TNF-alpha, and IL-6 in combination suggest a cytotoxic effector function. Gingival gamma delta T cells contained cytoplasmic electron-dense membrane-bound granules and multivesicular bodies that are ultrastructural characteristics of cytotoxic cells. Epithelial cells from inflamed gingiva expressed HLA-DR, CD1a, CD1c, and heat shock protein 60 on the cell surface. They also expressed mRNA for IL-1 beta, IL-6, IL-8, TNF-alpha, and TGF-beta 1. Thus, epithelial cells may function as accessory cells in immune activation and, at the same time, be target cells for CD8+ gamma delta T cells reactive with CD1 Ag or heat shock protein. These results suggest that gamma delta T cells constitute a first line of defense in gingiva, preventing entrance of pathogens by cytotoxicity against infected and stressed epithelial cells, and by control of epithelial cell growth through secretion of regulatory cytokines.
- Published
- 1994
22. Expression of carcinoembryonic antigen and nonspecific cross-reacting 50-kDa antigen in human normal and cancerous colon mucosa: comparative ultrastructural study with monoclonal antibodies
- Author
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V, Baranov, M M, Yeung, and S, Hammarström
- Subjects
Adult ,Membrane Glycoproteins ,Microvilli ,Colon ,Antibodies, Monoclonal ,Adenocarcinoma ,Capillaries ,Carcinoembryonic Antigen ,Antibody Specificity ,Antigens, Neoplasm ,Reference Values ,Antigens, Surface ,Colonic Neoplasms ,Humans ,Intestinal Mucosa ,Microscopy, Immunoelectron ,Cell Adhesion Molecules - Abstract
The precise localization of carcinoembryonic antigen (CEA) and non-specific cross-reacting 50-kDa antigen (NCA 50) in normal colon mucosa and colon adenocarcinoma was investigated by using an indirect immunoperoxidase electron microscopic technique with specific monoclonal antibodies. In normal adult colon both antigens were localized to microvesicles and filaments of the "fuzzy coat" on the apical surface of the epithelial cells. In addition, NCA 50 was found in the narrow spaces between adjoining microvilli. Mature columnar cells at the free luminal surface contained most of the antigen positive material. CEA and NCA 50 were also detected as intracellular components of goblet cells. In multilayered tumor glands, the cell surface expression of the antigens was dependent on the position of the tumor cell in the gland. The neoplastic cells showed either a predominant apical labeling or a positive staining of almost the entire cell surface. Some of the neoplastic cells contained CEA in so-called "intracellular lumina." In contrast to normal colon epithelial cells most tumor cells synthesized NCA 50 actively. In normal colonic mucosa, unlike in cancerous tissue, CEA and NCA 50 appear to be released via vesicles formed from the microvillous membrane of mature columnar cells. These results are consistent with the hypothesis that CEA and NCA play a role in the nonspecific defense against microorganisms in the large intestine.
- Published
- 1994
23. Human decidual leukocytes from early pregnancy contain high numbers of gamma delta+ cells and show selective down-regulation of alloreactivity
- Author
-
L, Mincheva-Nilsson, S, Hammarström, and M L, Hammarström
- Subjects
Antigens, Differentiation, T-Lymphocyte ,CD3 Complex ,Receptors, Antigen, T-Cell ,Receptors, Antigen, T-Cell, gamma-delta ,Flow Cytometry ,Immunohistochemistry ,CD56 Antigen ,Antigens, CD ,Pregnancy ,T-Lymphocyte Subsets ,Antigens, Surface ,Decidua ,Immune Tolerance ,Humans ,Female - Abstract
The mononuclear lymphoid cell population in human pregnant uterus mucosa, decidua, from early normal pregnancies was studied phenotypically and functionally. The phenotype was determined in situ by immunohistochemistry, and in isolated decidual mononuclear cell preparations by immunofluorescence and flow cytometry. A mild isolation procedure of gentle mechanical disruption followed by density gradient centrifugation was used. Leukocytes comprised a large part of the decidual tissue. They were present in aggregates mainly situated adjacent to the glandular epithelium. In addition, individual leukocytes were present intraepithelially, as well as scattered between the stromal cells and around vessels and lacunes. Four lymphocyte populations of approximately the same size were identified: TCR gamma delta+/CD56+ cells, TCR gamma delta+/CD56- cells, TCR gamma delta-/CD56+ cells, and TCR alpha beta+/CD8+ cells. TCR gamma delta- expressing cells comprised about 60% of the T cells. They were CD4-/CD8-, and about half of the TCR gamma delta+ cells expressed the memory/activation marker CD45RO. The Kp 43 Ag, earlier described on activated CD56+ and TCR gamma delta+ cells in peripheral blood, was essentially only expressed on the TCR gamma delta-/CD56+ cell population in decidua. At least 50% of the TCR alpha beta+ cells were CD8+. The function(s) of either one of these populations might be to prevent immunologic reactions against the fetus, to protect the uterus from unwanted extensive invasion of trophoblasts, or to protect the uteroplacental unit from infection. Decidual T cells did not respond to stimulation by alloantigens or mitogenic anti-CD3 mAb but responded to the same extent as PBMC to mitogenic lectins. The surface density of the TCR/CD3 complex was low on freshly isolated decidual lymphocytes, but could be up-regulated upon stimulation with PMA/Ionomycin. Local selective down-regulation of surface expression of the TCR/CD3 complex and of activation involving this complex might be one of the mechanisms by which a maternal immunologic reaction against the semiallogeneic fetus is prevented.
- Published
- 1992
24. Relationship between histamine, lipoxygenase and cyclooxygenase products in antigen-induced contraction in guinea-pig tracheal tube preparations
- Author
-
Rolf G. G. Andersson, Nils Grundström, Eva Lindström, and S. Hammarström
- Subjects
medicine.medical_specialty ,Leukotrienes ,Contraction (grammar) ,Indoles ,Health, Toxicology and Mutagenesis ,Mepyramine ,Guinea Pigs ,Indomethacin ,Toxicology ,Epithelium ,Histamine receptor ,chemistry.chemical_compound ,Internal medicine ,medicine ,Animals ,Antigens ,Pharmacology ,Pyrilamine ,Leukotriene ,biology ,Muscle, Smooth ,respiratory system ,Trachea ,medicine.anatomical_structure ,Endocrinology ,chemistry ,biology.protein ,Quinolines ,Respiratory epithelium ,Eicosanoids ,Leukotriene Antagonists ,Female ,Cyclooxygenase ,Propionates ,Histamine ,medicine.drug ,Muscle Contraction - Abstract
We have used a tracheal tube preparation to study antigen-induced contraction in sensitized guinea pig airways. Treatment with both the cyclooxygenase inhibitor indomethacin and the lipoxygenase inhibitor MK-886 (L-663,536) affected this contraction in preparations with intact epithelium. Indomethacin potentiated and MK-886 inhibited part of the contraction. Leukotriene release from tracheal tubes was measured after antigen challenge, and was found to be significant in preparations with an intact epithelium. When the epithelium was removed, the histamine receptor antagonist mepyramine reduced antigen-induced contraction by 90%. Our results show that when the epithelium is absent, histamine is the most important mediator in the contraction. With the epithelium left intact, the contraction is more complex: both the cyclooxygenase and lipoxygenase pathways are involved, and our Findings indicate that eicosanoid production is associated with the airway epithelium.
- Published
- 1992
25. Specificity and affinity of monoclonal antibodies against carcinoembryonic antigen
- Author
-
M, Nap, M L, Hammarström, O, Börmer, S, Hammarström, C, Wagener, S, Handt, M, Schreyer, J P, Mach, F, Buchegger, and S, von Kleist
- Subjects
Adult ,Male ,Colon ,Antibody Affinity ,Antibodies, Monoclonal ,Cross Reactions ,Flow Cytometry ,Carcinoembryonic Antigen ,Immunoenzyme Techniques ,Antibody Specificity ,Colonic Neoplasms ,Humans ,Female ,Aged - Abstract
The binding specificities of 52 well-characterized monoclonal antibodies (Mabs) against carcinoembryonic antigen (CEA) from 12 different research groups were studied by immunohistochemistry and immuno flow cytometry. In addition, the binding constant for the interaction between Mab and CEA was determined by a solution-phase assay. Cryostat sections of colon carcinoma and normal colon, stomach, liver, pancreas, and spleen were studied by immunohistochemistry. Peripheral blood granulocytes, monocytes, and lymphocytes were assayed by immuno flow cytometry. The Mabs used here have previously been classified into five essentially nonoverlapping epitope groups (GOLD 1-5) (Cancer Res., 49: 4852-4858, 1989). Most Mabs cross-reacted with different normal tissues, ranging from highly cross-reactive Mabs (positive reaction with 8 of 9 discriminating tissues) to relatively specific Mabs (positive reaction with 1 of 9 discriminating tissues). Five Mabs (10%) were specific, reacting only with colon carcinoma, normal colon mucosa, and normal gastric foveola. There was a correlation between epitope group and binding specificity. Mabs with a high degree of CEA specificity almost exclusively belonged to epitope groups 1, 2, and 3, while highly cross-reactive Mabs belonged to epitope groups 4 and 5. There was no correlation between antibody specificity and affinity for CEA. Specific Mabs with high as well as low affinity were found.
- Published
- 1992
26. Biosynthesis of carcinoembryonic antigen (CEA) gene family members expressed in human tumor cell lines: evidence for cleavage of the glycosyl phosphatidyl inositol (GPI) anchor by GPI-PLC and GPI-PLD
- Author
-
W N, Khan and S, Hammarström
- Subjects
Molecular Weight ,Membrane Glycoproteins ,Transcription, Genetic ,Antigens, CD ,Antigens, Neoplasm ,Multigene Family ,Biomarkers, Tumor ,Tumor Cells, Cultured ,Humans ,Phosphatidylinositols ,Cell Adhesion Molecules ,Carcinoembryonic Antigen ,Glycoproteins - Abstract
Three Carcinoembryonic Antigen (CEA) gene family members: CEA, Non-specific cross-reactive antigen 50/90 (NCA) and biliary glycoprotein (BGP) were expressed in the colon carcinoma cell lines LS174T and HT29. The CEA, NCA50/90 and four alternatively spliced BGP transcripts (BGP a-d) were identified. The molecular weights of the mature glycoproteins were: CEA, 180kD; NCA50/90, 70-100 kD; BGP, 85, 120 and 140 kD. Pulse chase experiments demonstrated that CEA first appears as a 165 kD high mannose precursor which is trimmed to a 160 kD intermediate and finally transformed into the mature 180 kD glycoprotein. The precursor form of NCA had a molecular weight of 50 kD. CEA and NCA50/90, but not BGP, were linked to the cell membrane via glycosyl phosphatidyl inositol and could be released from the intact tumor cells by glycosyl phosphatidyl inositol-specific phospholipase C. CEA on isolated membranes and in cell lysates, but not on intact cells, was also cleaved by fresh human serum or purified glycosyl phosphatidyl inositol-specific phospholipase D.
- Published
- 1991
27. Leukotriene C4 synthase: characterization in mouse mastocytoma cells
- Author
-
M, Söderström, B, Mannervik, and S, Hammarström
- Subjects
Male ,Chromatography ,Mast-Cell Sarcoma ,Mice, Inbred Strains ,Cell Line ,Kinetics ,Mice ,Durapatite ,Microsomes ,Chromatography, Gel ,Animals ,Female ,Indicators and Reagents ,Hydroxyapatites ,Glutathione Transferase - Published
- 1990
28. Tu-P7:85 5-lipoxygenase activity is involved in platelet-induced fibroblast proliferation
- Author
-
Torbjörn Bengtsson, E. Lindström, M. Söderström, S. Hammarström, H. Herbertsson, C. Berg, and A.-C. Svensson
- Subjects
biology ,Chemistry ,Fibroblast growth factor receptor 2 ,General Medicine ,Fibroblast growth factor receptor 4 ,Fibroblast growth factor receptor 3 ,Cell biology ,medicine.anatomical_structure ,Arachidonate 5-lipoxygenase ,Internal Medicine ,medicine ,biology.protein ,Platelet ,Cardiology and Cardiovascular Medicine ,Fibroblast - Published
- 2006
29. The impact of a new structured triglyceride emulsion on clinical chemistry, nitrogen metabolism and platelet phospholipids in postoperative patients
- Author
-
Johan Permert, S. Hammarström, Jörgen Larsson, S. Skullman, and Mikael Wirén
- Subjects
chemistry.chemical_compound ,Nutrition and Dietetics ,Triglyceride ,chemistry ,Biochemistry ,Emulsion ,Platelet ,Critical Care and Intensive Care Medicine ,Nitrogen cycle - Published
- 1993
30. IMBALANCE OF IFN-γ/TGFβ mRNA AND LOW LEVELS OF IL-2 AND TNFα EXPRESSED BY SMALL INTESTINAL T LYMPHOCYTES IN CHILDREN WITH UNTREATED CELIAC DISEASE
- Author
-
G. Forsberg, A. Israelsson, M.-L. Hammarström, Olle Hernell, and S. Hammarström
- Subjects
Messenger RNA ,business.industry ,Pediatrics, Perinatology and Child Health ,Immunology ,Gastroenterology ,Medicine ,Tumor necrosis factor alpha ,Disease ,business - Published
- 1999
31. Conversion of 5,8,11-eicosatrienoic acid to leukotrienes C3 and D3
- Author
-
S Hammarström
- Subjects
chemistry.chemical_classification ,Leukotriene ,Kidney ,Stereochemistry ,Ionophore ,Mastocytoma ,Cell Biology ,medicine.disease ,Biochemistry ,chemistry.chemical_compound ,Enzyme ,medicine.anatomical_structure ,chemistry ,Cell culture ,medicine ,Arachidonic acid ,Molecular Biology ,Cis–trans isomerism - Abstract
5,8,11-Eicosatrienoic acid was converted by mouse mastocytoma cells stimulated with ionophore A23187 to two slow reacting substances. These were characterized by spectroscopy and by chemical and enzymatic degradations as two geometrical isomers of 5-hydroxy-6-S-glutathionyl-7,9,11-eicosatrienoic acid (E,E,Z; leukotriene C3 and E,E,E; 11-trans-leukotriene C3). Corresponding cysteinylglycine compounds (leukotriene D3 and 11-trans leukotriene D3) were obtained from the leukotriene C3 isomers by treatment with kidney gamma-glutamyl transpeptidase. The biological effects of leukotrienes C3 and D3, on the isolated guinea pig ileum, were approximately the same as of leukotrienes derived from arachidonic acid.
- Published
- 1981
32. Metabolism of leukotriene C3 in the guinea pig. Identification of metabolites formed by lung, liver, and kidney
- Author
-
S Hammarström
- Subjects
medicine.medical_specialty ,Leukotriene ,Chemistry ,Catabolism ,Feces analysis ,Kidney metabolism ,Cell Biology ,Metabolism ,Glutathione ,Biochemistry ,Guinea pig ,chemistry.chemical_compound ,Endocrinology ,Internal medicine ,medicine ,Molecular Biology ,Cysteine - Abstract
[5,6,8,9,11,12-3H6]Leukotriene C3 was converted to polar metabolites which ere excreted in feces and urine for 4-5 days after subcutaneous administration to guinea pigs. Lung homogenates converted leukotriene C3 to leukotriene D3. Liver and kidney homogenates did not catabolize leukotriene C3 appreciably due to inhibition of gamma-glutamyl transpeptidase in these tissues by endogenous glutathione. Liver and kidney homogenates metabolized [3H6]leukotriene D3 to the cysteine analog, leukotriene E3 (Bernstrom, K., and Hammarstrom, S. (1981) J. Biol. Chem. 256, 9579-9582). In addition leukotriene C3 and products of greater polarity were formed. The conversion of leukotriene D3 to leukotriene C3 was catalyzed by gamma-glutamyl transpeptidase which performed a reverse reaction due to the high tissue concentrations of glutathione. The results suggest that the degradation of leukotriene C3 to leukotriene D3 is important for the further metabolism of this leukotriene in liver and kidney.
- Published
- 1981
33. Stereospecific elimination of hydrogen at C-10 in eicosapentaenoic acid during the conversion to leukotriene C5
- Author
-
S Hammarström
- Subjects
Leukotriene biosynthesis ,Leukotriene ,Stereospecificity ,Hydrogen ,Chemistry ,Leukotriene C5 ,Stereochemistry ,chemistry.chemical_element ,Tritium ,Cell Biology ,Molecular Biology ,Biochemistry ,Eicosapentaenoic acid - Abstract
(10L)- and (10D)-[1-14C, 10-3H]5,8,11,14,17-eicosapentaenoic acids were synthesized to investigate mechanistic and stereochemical aspects of leukotriene biosynthesis. Experiments with mastocytoma cells showed that a hydrogen is stereospecifically eliminated from C-10 during the conversion of eicosapentaenoic acid to leukotriene C5. The hydrogen lost has the pro-S (D) configuration. 5-Hydroxy-6,8,11,14,17-eicosapentaenoic acid, formed in the same experiments, was enriched in tritium when the (10D), but not when the (10L), isomer of labeled eicosapentaenoic acid was used. This indicates that oxygenation of the acid at C-5 occurred before the elimination of hydrogen and suggests that removal of the pro-S hydrogen at C-10 in 5-hydroperoxy-6,8,11,14,17-eicosapentaenoic acid initiates its transformation to trans-5(S),6(S)-oxido-7,9-trans-11,14,17-cis-eicosapentaenoic acid (leukotriene A5).
- Published
- 1983
34. Metabolism of leukotriene D by porcine kidney
- Author
-
S Hammarström and K Bernström
- Subjects
chemistry.chemical_classification ,Leukotriene ,Leukotriene E4 ,Leukotriene D4 ,Leukotriene C4 ,Metabolite ,Kidney metabolism ,Cell Biology ,Metabolism ,Biochemistry ,chemistry.chemical_compound ,Enzyme ,chemistry ,Molecular Biology - Abstract
An enzyme from porcine kidney converted leukotriene D4 into a less polar metabolite. The structure of this compound was 5-hydroxy-6-S-cysteinyl-7,9-trans-11,14-cis-eicosatetraenoic acid (leukotriene E4). Analogous products, viz. 5-hydroxy-6-S-cysteinyl-7,9,11-eicosatrienoic acid (leukotriene E3), 5-hydroxy-6-S-cysteinyl-7,9,11-trans-14-cis-eicosatetraenoic acid (11-trans-leukotriene E4), and 5-hydroxy-6-S-cysteinyl-7,9,11,14,17-eicosapentaenoic acid (leukotriene E5) were formed from leukotrienes D3, 11-trans-D4, and D5, respectively. Leukotriene E4 induced slow reacting substance-like contractions of guinea pig ileum but was less potent (8-12 times) than leukotriene C4. The biological potency of 11-trans-leukotriene E4 was similar to that of leukotriene E4.
- Published
- 1981
35. Enzymatic synthesis of 15-hydroperoxythromboxane A2 and 12-hydroperoxy-5,8,10-heptadecatrienoic acid
- Author
-
S. Hammarström
- Subjects
chemistry.chemical_classification ,biology ,Substrate (chemistry) ,Cell Biology ,Biochemistry ,Chloride ,Thromboxane B2 ,chemistry.chemical_compound ,Enzyme ,chemistry ,biology.protein ,medicine ,Organic chemistry ,Platelet ,Thromboxane-A synthase ,Methanol ,Molecular Biology ,Prostaglandin G2 ,medicine.drug - Abstract
Partially purified thromboxane synthase from human platelets converted prostaglandin G2 to two products. These were transformed to 12-hydroxy-5,8,10-heptadecatrienoic acid and thromboxane B2 by stannous chloride reduction and to 12-keto-5,8,10-heptadecatrienoic acid and 15-ketothromboxane B2 by lead tetraacetate dehydration. Trapping with methanol after short incubations of substrate with enzyme and reduction by stannous chloride yielded 11-O-methylthromboxane B2. Based on these results, the enzymatically formed products were identified as 12-hydroperoxy-5,8,10-heptadecatrienoic acid and 15-hydroperoxythromboxane A2.
- Published
- 1980
36. Induction of aggregation and enhancement of proliferation and IL-2 secretion in human T cells by antibodies to CD43
- Author
-
B Axelsson, R Youseffi-Etemad, S Hammarström, and P Perlmann
- Subjects
Immunology ,Immunology and Allergy - Abstract
CD43 (large sialoglycoprotein) is a heavily glycosylated protein expressed on virtually all thymus-derived lymphocytes, on a subpopulation of B cells and on granulocytes. Recently, an anti-CD43 mAb (L10) was shown to induce proliferation in T cells comparable to that induced by anti-CD3. The L10 antibody was reported to react with both sialylated and desialylated CD43. In order to further elucidate the role of CD43 in various T cell functions we have studied the biologic properties of two other mAb (B1B6 and E11B, IgG1) directed against sialic acid-dependent epitopes on CD43. Addition of low amounts of antibody (5 to 10 ng/ml) to freshly isolated T cells or to T cell lines resulted in a rapid clustering of the cells. Fab fragments were also active albeit at a 10-fold higher concentration. Aggregation was dependent on active cell metabolism (inhibited by azide and at low temperatures), on the presence of divalent cations (Mg2+) and was inhibited by antibodies to CD18 but not by antibodies to CD11a (leukocyte function-associated Ag-1 alpha). B1B6 and E11B were poorly mitogenic when added alone in soluble form to PBL or to T cells. However, supernatants from cultures of PBL treated with B1B6 for 2 days contained IL-2 activity. No increase in the number of CD25+ cells was seen during the same period. Exogenously added IL-2 did not synergize with B1B6 or E11B in activation of PBL, whereas proliferation was significantly increased by the addition of the antibodies to activation systems with low endogenous production of IL-2 (PMA or soluble anti-CD3). The anti-CD43 antibodies amplified T cell proliferative responses induced by Con A or leukoagglutinin from Phaseolus vulgaris. F(ab')2 fragments enhanced proliferation significantly better than Fab fragments suggesting that cross-linking of CD43 molecules was an essential features of the amplifying signal. Compared with cultures activated by Con A alone, an increased number of CD25+ cells and of blast cells as well as an increased IL-2 production was observed in cultures activated by B1B6-Con A. The results indicate that regulatory signals, which may function to modify homo- or heterotypic T cell adhesion as well as autocrine production of IL-2, can be transduced through CD43.
- Published
- 1988
37. Cea and nca in amniotic fluid of normal and abnormal pregnancies
- Author
-
S. Hammarström, H. Gadler, Britta Wahren, and K. Bremme
- Subjects
Cancer Research ,medicine.medical_specialty ,Amniotic fluid ,Text mining ,Oncology ,business.industry ,Obstetrics ,Medicine ,business - Published
- 1978
38. Distribution and metabolism of 3H-labeled leukotriene C3 in the mouse
- Author
-
L E Appelgren and S Hammarström
- Subjects
medicine.medical_specialty ,Leukotriene ,Kidney ,Lung ,Chemistry ,Connective tissue ,Kidney metabolism ,Spleen ,Cell Biology ,Biochemistry ,Small intestine ,Excretion ,medicine.anatomical_structure ,Endocrinology ,Internal medicine ,medicine ,Molecular Biology - Abstract
The distribution of [5,6,8,9,11,12-3H6]leukotriene C3 in mice was determined by whole body autoradiography of sagittal sections. Tritium was rapidly eliminated from the circulation by uptake in liver and excretion in bile as well as by renal uptake and excretion in urine. Chromatographic analyses of tritium in tissues and body fluids indicated that leukotrienes C3 and D3 constituted significant parts of the radioactivity in liver, bile, and small intestine 20 min after intravenous injection, whereas kidney contained leukotriene E3 and unidentified metabolites. Lung, pleural fluid, spleen, salivary glands, and connective tissue showed higher concentrations of tritium than blood. The radioactivity in lung consisted predominantly of leukotriene E3 with smaller amounts of leukotrienes C3 and D3.
- Published
- 1982
39. Prostaglandins
- Author
-
B, Samuelsson, E, Granström, K, Green, M, Hamberg, and S, Hammarström
- Subjects
Blood Platelets ,Binding Sites ,Chromatography, Gas ,Receptors, Drug ,Cell Membrane ,Radioimmunoassay ,Bone Marrow Cells ,Muscle, Smooth ,Fibroblasts ,Biochemistry ,Mass Spectrometry ,Peroxides ,Neuroblastoma ,Exocrine Glands ,Adipose Tissue ,Bone Marrow ,Prostaglandin-Endoperoxide Synthases ,Prostaglandins ,Cyclooxygenase Inhibitors ,Enzyme Inhibitors - Abstract
Since the literature on PGs (prostaglandins) is increasing so rapidly, this literature review covers only the following PG-related topics: 1) PG biosynthesis; 2) PG metabolism; 3) analysis of PGs; 4) PG receptors; 5) PGs and cyclic nucleotides; and 6) the effect of PGs on platelet function. PG biosynthesis has been monitored by labeled precursor acids and chromatographic identification, gas-liquid chromatography, radioimmunoassay and multiple ion analysis, and measurement of urinary metabolites. These methods have shown that many mammalian tissues produce PGs. Various conditions and agents have been found to alter the rate of PG synthesis; aspirin is 1 agent which inhibits PG biosynthesis. The metabolic pathways of PGs are diagrammed chemically. The highly sensitive methods for performing quantitative analysis of PGs which have been developed in recent years are explained. These include gas chromatography with electron capture detectors, gas chromatography/mass spectrometry, and radio-immunoassay. PGs are known to react with adenyl cyclase in many different tissues. This may explain their wide variety of pharmacological effects. Platelet aggregation is stimulated in its 2nd stage by PGE2 and inhibited by PGE1.
- Published
- 1975
40. Helix pomatia A hemagglutinin, a surface marker for bovine T-lymphocytes
- Author
-
S. Hammarström, U. Hellström, Bror Morein, L-G. Axelsson, and C. Johansson
- Subjects
Rosette Formation ,General Veterinary ,biology ,Helix, Snails ,T-Lymphocytes ,Immunology ,Cell ,Lectin ,Cell Separation ,Fractionation ,Helix pomatia ,Hemagglutinin ,biology.organism_classification ,Fluorescence ,Molecular biology ,Sepharose ,Hemagglutinins ,medicine.anatomical_structure ,Lectins ,medicine ,biology.protein ,Animals ,Cattle ,Neuraminidase ,Biomarkers - Abstract
Bovine peripheral blood lymphocytes (PBL) were isolated from blood collected from 6 cattle. After treatment with neuraminidase, 40 or 60% of the cells were shown to combine with Helix Pomatia A hemagglutinin (HP) depending whether a direct or indirect fluorescence technique was used. About 20% of the cells were Ig-bearing. With double staining fluorescence technique, it was shown that cells attaching to HP were not Ig-bearing and the reverse. With the aid of HP, covalently bound to Sepharose, Ig-bearing cells could be separated from cell populations attaching to HP. The fraction of cells forming rosettes with sheep erythrocytes was proportional to that of HP attaching cells both before and after fractionation on the HP column. It is therefore concluded that HP is a marker for bovine T-cells, and that this lectin may be used to separate B-cells from T-cells.
- Published
- 1979
41. Antibody-Induced Hemolytic Activity of Human Blood Monocytes
- Author
-
S. Hammarström, J. B. Natvig, E. Engwall, and G. Holm
- Subjects
Lysis ,biology ,Myeloma protein ,Immunology ,General Medicine ,medicine.disease ,Molecular biology ,Cold Agglutinin ,Hemolysis ,Subclass ,Lytic cycle ,parasitic diseases ,biology.protein ,medicine ,Antibody ,Receptor - Abstract
The hemolytic effect of purified human blood monocytes was quantitated as the release of isotope from human erythrocytes labeled with 51Cr-chromate. We here demonstrate that IgM antibody (anti-A or cold agglutinin) was unable to confer monocyte-mediated hemolysis, and that lysis was induced by IgG anti-A antibody. In addition, IgG anti-D sera containing antibodies belonging to subclass IgG1 or IgG3 had approximately equal hemolytic efficiency, as calculated from the antigen-binding capacity of the sera at the concentrations inducing 50% monocyte-mediated hemolysis. The lytic reaction was suppressed by pooled IgG or by individual myeloma proteins of subclass IgGl or IgG3. The inhibitory effect of heat-aggregated IgG was not different from that of native IgG. IgG2 or IgG I had no or weak effects. Cross-inhibition of lysis induced by IgG1 and IgG3 anti-D antibody showed strong and equal suppression by IgG1 and IgG3 myeloma proteins. Hundredfold higher concentrations of IgG2 or IgG4 was required for inhibition of lysis, which may be an effect of contaminating IgGl or IgG3. Our data strongly suggest that the receptors on human blood monocytes for IgGl and IgG3 that participate in monocyte-mediated hemolysis are identical.
- Published
- 1974
42. Leukotrienes and myometrial activity of the term pregnant uterus
- Author
-
Anders Norström, Bryman I, Bo Lindblom, S. Hammarström, M. Wikland, and Nils Wiqvist
- Subjects
medicine.medical_specialty ,Pregnancy Trimester, Third ,Cervix Uteri ,In Vitro Techniques ,Biology ,Biochemistry ,Uterine contraction ,Contractility ,Uterine Contraction ,chemistry.chemical_compound ,Endocrinology ,Pregnancy ,Internal medicine ,medicine ,Humans ,Cervix ,Leukotriene ,Leukotriene C4 ,Myometrium ,Biological activity ,respiratory system ,medicine.disease ,Pregnancy Trimester, First ,medicine.anatomical_structure ,chemistry ,Female ,SRS-A ,lipids (amino acids, peptides, and proteins) ,medicine.symptom - Abstract
Biopsies from different segments of the pregnant human uterus were superfused in organ chambers and contractile activity was registered. Leukotriene C4(LTC4) caused inhibition of spontaneous but not noradrenaline induced contractile activity in strips from the cervix. This effect occurred both in early pregnancy and at term. However, the lower and the upper uterine segment of the term pregnant uterus did not respond to LTC4. The results represent a documentation of the segmental differentiation in the uterine response to eicosanoids.
- Published
- 1985
43. An Antigenic Relationship Between Human Kidney, Colon and the Common Antigen of Enterobacteriaceae
- Author
-
U. Jodal, S.E. Holm, J. Ahlmén, S. Hammarström, J. Holmgren, and P.O. Attman
- Subjects
Immunodiffusion ,Erythrocytes ,Colon ,Immunology ,Cross Reactions ,Kidney ,Absorption ,Microbiology ,Fetus ,Enterobacteriaceae ,Antigen ,Escherichia coli ,Humans ,Immunology and Allergy ,Antigens ,Antigens, Bacterial ,biology ,Immune Sera ,Human kidney ,General Medicine ,Hemagglutination Inhibition Tests ,biology.organism_classification ,Liver ,Colitis, Ulcerative - Abstract
By immunodiffusion analyses an antigen was demonstrated in human kidney which cross-reacted with colon antigen from germ-free rats as well as with the common antigen of Enterobacteriaceae extracted from E. coli O14 bacteria. The possibility is raised that the progressive renal disease sometimes noted in patients with ulcerative colitis might be due to autoantibodies or cells with immunologic reactivity against kidney as well as colon.
- Published
- 1972
44. Gas–liquid chromatography–mass spectrometry of synthetic ceramides containing 2-hydroxy acids
- Author
-
S Hammarström, Bengt Samuelsson, and K Samuelsson
- Subjects
chemistry.chemical_classification ,Ceramide ,Chromatography ,Sphingosine ,Fatty acid ,QD415-436 ,Cell Biology ,Mass spectrometry ,Biochemistry ,chemistry.chemical_compound ,Residue (chemistry) ,Endocrinology ,chemistry ,Mass spectrum ,Gas chromatography ,Electron ionization - Abstract
Ceramides containing either sphingosine or sphinganine and one of the 2-hydroxy acids, 14h:0, 16h:0, 18h:0, 20h:0, 22h:0, 24h:0, and 26h:0 were prepared and separated by gas chromatography as the 1,3,2'-tri-O-tri-methylsilyl derivatives. Mass spectrometric analyses of these derivatives showed that the ions formed on electron impact can be used to determine unequivocally the structures of the long-chain base and the fatty acid residue in the ceramide. Proposed structures of ions and the mechanisms of reaction of their formation are supported by mass spectra of homologous derivatives, by deuterium labeling experiments, and by high-resolution on mass spectrometry.
- Published
- 1970
45. Über das Vorkommen des Prostaglandins in Tierorganen1
- Author
-
S. Hammarström and U. S. v. Euler
- Subjects
Physiology ,Biology - Published
- 1937
46. Über Vorkommen und Wirkung von Adrenalin in Ovarien1
- Author
-
S. Hammarström and U. S. v. Euler
- Subjects
Physiology ,Chemistry - Published
- 1937
47. Formation of 5,5-dimethyl-delta2-thiazoline-4-carboxylic acid during cleavage of penicillin G by D-alanine carboxypeptidase from Bacillus stearothermophilus
- Author
-
S Hammarström and J L Strominger
- Subjects
chemistry.chemical_classification ,Stereochemistry ,Carboxylic acid ,Thiazoline ,Cell Biology ,Cleavage (embryo) ,Biochemistry ,Penicillin ,chemistry.chemical_compound ,chemistry ,medicine ,D-alanine carboxypeptidase ,Molecular Biology ,medicine.drug - Published
- 1976
48. IgE-mediated activation of human heartin vitro
- Author
-
Massimo Triggiani, S. Hammarström, Gianni Marone, Raffaele Cirillo, Attilio Giacummo, Mario Condorelli, Marone, Gianni, M., Triggiani, R., Cirillo, A., Giacummo, S., Hammarstrom, and M., Condorelli
- Subjects
Allergy ,medicine.medical_specialty ,Immunology ,Dose-Response Relationship, Immunologic ,Arachidonic Acids ,In Vitro Techniques ,Toxicology ,Immunoglobulin E ,Histamine Release ,chemistry.chemical_compound ,Internal medicine ,medicine ,Animals ,Humans ,Pharmacology (medical) ,Secretion ,Incubation ,Pharmacology ,Arachidonic Acid ,Leukotriene C4 ,biology ,Prostaglandin D2 ,Prostaglandins D ,Myocardium ,medicine.disease ,In vitro ,Thromboxane B2 ,Endocrinology ,chemistry ,Anesthesia ,biology.protein ,Rabbits ,Histamine - Abstract
We used human cardiac tissue from the right atrial appendages of patients undergoing corrective heart surgery to study content and de novo synthesis of mediators in the human heart. Human heart tissue contained 1.7 +/- 0.1 micrograms/g wet weight of histamine (mean +/- S.E.M.) and spontaneously produced 6-keto-PGF1 alpha (38.4 ng/g wet weight/min), PGF1 alpha (1.9 ng/g wet weight/min), PGE (1.7 ng/g wet weight/min) and thromboxane B2 (TxB2) (1.7 ng/g wet weight/min). Spontaneous release of PGD2, leukotriene C4 and histamine was negligible. Rabbit anti-human IgE (1-10 micrograms/ml) dose-dependently induced the release of histamine (5 to 15% of the total histamine content) and of PGD2 (5 to 100 ng/g of wet tissue). The effect of anti-IgE was dose-related and reached a maximum after 30-45 min of incubation. A significant linear correlation (rs = 0.90; p less than 0.001) was found between de novo synthesis of PGD2 and the secretion of histamine induced by anti-IgE challenge of human heart. These results support the concept that PGI2 is the main, but not the sole, product of arachidonic acid metabolism synthesized by human heart in vitro. Additionally, anti-IgE challenge of human heart in vitro induces the release of histamine and PGD2. The local concentrations of these mediators appear high enough to play some role in the modulation of several cardiac functions in vivo.
- Published
- 1986
49. Conversion of dihomo-gamma-linolenic acid to an isomer of leukotriene C3, oxygenated at C-8
- Author
-
S. Hammarström
- Subjects
Leukotriene ,Chemistry ,Stereochemistry ,Ionophore ,medicine ,Mastocytoma ,Cell Biology ,Dihomo-gamma-linolenic Acid ,medicine.disease ,Molecular Biology ,Biochemistry - Abstract
Mouse mastocytoma cells stimulated with ionophore A23187 and dihomo-gamma-linolenic acid produced a novel leukotriene which was characterized as 8-hydroxy-9-S-glutathionyl-10,12,14-eicosatrienoic acid (8,9-leukotriene C3).
- Published
- 1981
50. Identification of leukotriene C-1 as a major component of slow-reacting substance from rat mononuclear cells
- Author
-
M K Bach, J R Brashler, S Hammarström, and B Samuelsson
- Subjects
Immunology ,Immunology and Allergy - Abstract
Slow-reacting substance (SRS) was produced by rat peritoneal mononuclear cells after stimulation with the ionophore A23187. The SRS consisted of two main components as judged by high-pressure liquid chromatography (HPLC) on Florisil. The larger and more polar component consisted mainly of leukotriene (LT) C-1 as judged by ultraviolet spectroscopy, mass spectrometry (after desulfurization), amino acid analysis, and conversion by soybean lipoxygenase. Comparisons with authentic LTC-1 showed identity on reverse phase HPLC and guinea pig ileum bioassay. The latter methods are known to distinguish between LTC-1 and stereoisomers of LTC-1.
- Published
- 1980
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