1. Inhibiting phosphatase and actin regulator 1 expression is neuroprotective in the context of traumatic brain injury
- Author
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Jun Ding, Zhi-Ming Xu, Heng-Li Tian, Yao Jing, Lin Zhang, Shi-Wen Chen, Yan Guo, Shi-Ming Ju, Fang Yuan, Hao Chen, and Dian-Xu Yang
- Subjects
Developmental Neuroscience - Abstract
Studies have found that the phosphatase actin regulatory factor 1 expression can be related to stroke, but it remains unclear whether changes in phosphatase actin regulatory factor 1 expression also play a role in traumatic brain injury. In this study we found that, in a mouse model of traumatic brain injury induced by controlled cortical impact, phosphatase actin regulatory factor 1 expression is increased in endothelial cells, neurons, astrocytes, and microglia. When we overexpressed phosphatase actin regulatory factor 1 by injection an adeno-associated virus vector into the contused area in the traumatic brain injury mice, the water content of the brain tissue increased. However, when phosphatase actin regulatory factor 1 was knocked down, the water content decreased. We also found that inhibiting phosphatase actin regulatory factor 1 expression regulated the nuclear factor kappa B signaling pathway, decreased blood-brain barrier permeability, reduced aquaporin 4 and intercellular adhesion molecule 1 expression, inhibited neuroinflammation, and neuronal apoptosis, thereby improving neurological function. The findings from this study indicate that phosphatase actin regulatory factor 1 may be a potential therapeutic target for traumatic brain injury. more...
- Published
- 2022