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Background Colorectal cancer (CRC) is one of the most common malignant tumors of the digestive system, ranking third for morbidity and mortality worldwide. At present, no effective control method is available for this cancer type. In tumor cells, especially iron metabolization, is necessary for its growth and proliferation. High levels of iron are an important feature to maintain tumor growth; however, the overall mechanism remains unclear. Methods We used western blotting, immunohistochemistry (IHC) and real-time quantitative PCR to analyze the expression of IGF2BP2 in cell lines and tissues. Further, RNA-sequencing, RNA immunoprecipitation and methylated RNA immunoprecipitation experiments explored the specific binding of target genes. Moreover, the RNA stability assay was performed to determine the half-life of genes downstream of IGF2BP2. In addition, the Cell Counting Kit-8, colony formation assay, 5-ethynyl-2’-deoxyuridine assay and flow cytometry were used to evaluate the effects of IGF2BP2 on proliferation and iron metabolism. Lastly, the role of IGF2BP2 in promoting CRC growth was demonstrated in animal models. Results We observed that IGF2BP2 is associated with iron homeostasis and that TFRC is a downstream target of IGF2BP2. Further, overexpression of TFRC can rescue the growth of IGF2BP2-knockdown CRC cells. Mechanistically, we determined that IGF2BP2 regulates TFRC methylation via METTL4, thereby regulating iron metabolism and promoting CRC growth. Furthermore, using animal models, we observed that IGF2BP2 promotes CRC growth. Conclusion IGF2BP2 regulates TFRC mRNA methylation via METTL4, thereby regulating iron metabolism and promoting CRC growth. Our study highlights the key roles of IGF2BP2 in CRC carcinogenesis and the iron transport pathways.

The leadership style of head nurses affects the organizational atmosphere of nursing teams.The aim of this study was to analyze the correlation between head nurse leadership styles and nurses' morale and intention to stay, as well as the explained variance for each.The descriptive correlational design employed in this study used a convenience sample of 790 nursing staff working at a medical center in southern Taiwan. We cross-sectionally surveyed each participant's intention to stay, morale, and perception of their head nurse's leadership style.The participants perceived their head nurses as having both transformational and transactional leadership styles. A moderate to highly positive correlation was identified among leadership style, morale, and intention to stay. One-way analyses of variance found that the participants who were seniors, were married, had children, were at clinical ladder N3 or above, had rotation experience, and held a public servant position had relatively higher morale and intention to stay. After controlling for potentially confounding factors, hierarchical regression analyses revealed that the explained variance of leadership styles on intention to stay and morale was 22% and 28%, respectively. Transformational leadership was found to significantly predict intention to stay and morale. However, transactional leadership significantly impacted morale only, albeit at a lower level than transformational leadership.How nurses perceive the leadership style of their head nurses may affect their intention to stay and morale while at work. Advanced training to strengthen and internalize leadership styles for head nurses is suggested. Creating a positive and friendly working environment is conducive to improving the morale of nurses and retention rates in the nursing workplace.領導風格對護理人員工作士氣及留任意願之相關性研究.護理長是單位的靈魂人物，領導風格影響護理團隊組織氛圍。.分析護理長領導風格對工作士氣和留任意願之間的相關性，以及對各自的解釋力。.採描述性相關研究設計，方便取樣南部某醫學中心790位護理人員，橫斷性調查留任意願、工作士氣及對護理長領導風格的評價。.護理師評價護理長兼具轉換型及交易型二種領導風格，領導風格與工作士氣及留任意願三者間呈中高程度正相關性。單因子變異數分析發現資深、已婚、有子女、N3以上、有輪調經驗、公職護理師留任意願及工作士氣相對較高。階層迴歸分析控制變異因素後發現領導風格對留任意願及工作士氣分別具22%及28%的解釋力。轉換型領導顯著影響留任意願及工作士氣；交易型領導僅對工作士氣有顯著影響，但相對低於轉換型領導。.護理人員對護理長領導風格的評價可能影響其留任意願及工作士氣，建議針對護理長領導能力的培訓需強化領導風格的內化，營造正向友善的工作環境，將有助於提高護理人員工作士氣及護理職場留任率。.

Summary: Purpose: Sleep quality was considered a priority concern facing pregnant women. Conventional wisdom argues that good sleep quality benefits pregnant women and their fetuses. The aim of this study is to assess the effects of a specific exercise program on the sleep quality in pregnant women.Methods: Searches were executed in seven databases since their inceptions until February 28, 2019, for randomized controlled trials evaluating the effects of an exercise program on the sleep quality and insomnia in pregnant women. A random-effects model was applied for meta-analysis, and odds ratio, mean differences (MDs), and 95% confidence intervals (CIs) are shown as parts of outcomes.Results: Seven studies were included for meta-analysis. Compared with their not-exercising counterparts, analyses showed that regularly exercising women had significantly enhanced sleep quality, with an odds ratio of 6.21 (95% CI, 2.02–19.11;p = .001; I2 = 80.2%), with a standardized MD of −0.93 (95% CI, −1.19 to −0.67; p < .001; I2 = 30.0%). However, exercising women showed no significant insomnia improvement, with an standardized MD of −2.85 (95% CI, −7.67 to 1.98; p = .250; I2 = 97.0%), relative to their not-exercising counterparts.Conclusion: This research indicated that exercise has a positive impact on the sleep quality of pregnant women. Despite the aforementioned positive impact on sleep quality, the present study did not find evidence to support that exercise may also improve insomnia for pregnant women. Keywords: exercise, meta-analysis, pregnant women, sleep, sleep initiation and maintenance disorders

Malignancies of alimentary tract include esophageal carcinoma (ESCA), stomach adenocarcinoma (STAD), colon adenocarcinoma (COAD), and rectum adenocarcinoma (READ). Despite of their similarities in cancer development and progression, there are numerous researches concentrating on single tumor but relatively little on their common mechanisms. Our study explored the transcriptomic data of digestive tract cancers from The Cancer Genome Atlas database, yielding their common differentially expressed genes including 1,700 mRNAs, 29 miRNAs, and 362 long non-coding RNAs (lncRNAs). There were 12 mRNAs, 5 miRNAs, and 16 lncRNAs in the core competitive endogenous RNAs network by RNA-RNA interactions, highlighting the prognostic nodes of SERPINE1, hsa-mir-145, and SNHG1. In addition, the weighted gene co-expression network analysis (WGCNA) illustrated 20 gene modules associated with clinical traits. By taking intersections of modules related to the same trait, we got 67 common genes shared by ESCA and READ and screened 5 hub genes, including ADCY6, CXCL3, NPBWR1, TAS2R38, and PTGDR2. In conclusion, the present study found that SERPINE1/has-mir-145/SNHG1 axis acted as promising targets and the hub genes reasoned the similarity between ESCA and READ, which revealed the homogeneous tumorigenicity of digestive tract cancers at the transcriptome level and led to further comprehension and therapeutics for digestive tract cancers.

Polyethylene/hexagonal boron nitride (PE/h-BN) composite sheets with enhanced thermal conductivity were fabricated through annealing a multilayered structure of alternating high density PE (HDPE)/h-BN composite and low density PE (LDPE) layers. Multilayer sheets possess a relatively low initial content filler, and after thermal annealing at 200 °C, the PE molecules in the composite layers diffused into neighboring layers. As a result, the h-BN concentration in the composite layers increased with the annealing time, resulting in the formation of thermal conduction pathways and enhancement of the final thermal conductivity. When the volume content of h-BN was 5.97 vol.%, the through-plane thermal conductivity of the annealed specimen was 1.37 W m−1 K−1, which showed an enhancement of ∼180% compared to the value of the neat composite with the same content of randomly dispersed h-BN. Our work suggests that this facile method is promising for the development of high-performance thermally conductive products.

Hyphenrones R–X (1–7), seven new polyprenylated acylphloroglucinols derivatives, were isolated from the fruits of Hypericum henryi, together with eight know analogues. Compounds 1 and 2 were elucidated to possess complex caged skeleton, while compounds 3–6 shared a common 3,9-epoxy moiety deriving from the normal polyprenylated acylphloroglucinols with a bicyclo[3.3.1]nonane-2,4,9-trione core. The new structures were elucidated on the basis of the interpretation of nuclear magnetic resonance (NMR) data, circular dichroism (CD) comparison, and single-crystal X-ray diffraction. In the bioassay, several compounds exhibited inhibitory activities against human tumor cell lines in vitro.

本研究旨在分析Montessori（1870-1952）的人格教育思想，主要運用詮釋學的研究方法：一方面聚焦於《吸收性心智》（The Absorbent Mind）、《童年的秘密》（The Secret of Childhood）、《發現兒童》（The Discovery of Child）及 《了解你的孩子》（What You Should Know about Your Child）等文本的理解，二方面來回於個人經驗與文本脈絡之間的對話與省思。結果發現，可歸納為以下四點：一、人格是精神生命肉身化的結果，人格統整的關鍵是意志發展。二、人格教育的目標在於建立紀律，人格教育的原則是給予自由，人格教育的方法是透過工作。三、人格教育的歷程始於兒童的自由選擇，繼之以專注地自發性工作，透過意志發展逐漸樂於服從，最後形成內在紀律，表現出寧靜的心境。四、人格教育的實施，關鍵時期為零至三歲，可以運用靜默遊戲、走線練習、穿脫衣服、準備茶會、舉辦生日會等方式進行。最後，本文更針對其思想內涵，提出10點評析，以供參考。 This study aims to analyze Maria Montessori’s theory of personality education. The author adopts interpretative methodology to focus on the following texts: The Absorbent Mind, The Secret of Childhood, The Discovery of the Child, and What You Should Know about Your Child. In this paper, the conclusion can be summarized into four points: First, personality is formed in the development of body and spirit, and the will is the key to integrate the whole system of personality. Second, the goal of personality education is to establish the discipline of life, and the method is to let children work spontaneously with the sense of duty in a free situation. Third, children must have the freedom to choose, then they can learn how to exercise their will to obey the inner discipline. Fourth, there are five examples provided for the implementation of personality education.

Hypercohin K (1), the first polycyclic polyprenylated acylphloroglucinol possessing a unique spiro-fused cyclopropane ring, together with hyphenrone L (2) representing the most complex congeners featured with a hexacyclic core, was isolated from Hypericum cohaerens. The structure of 1 was elucidated by extensive MS and NMR spectroscopic data. The absolute configuration was defined by comparison of the experimental and calculated ECD. Compound 1 was evaluated for the inhibitory activities against acetylcholinesterase and four human tumor cell lines. (C) 2015 Elsevier Ltd. All rights reserved.

Candida albicans is an opportunistic human fungal pathogen. The ability to switch among multiple cellular forms is key to its pathogenesis. The Dbf4-dependent protein kinase gene CDC7 is conserved due to its role in initiating DNA replication. Because a C. albicans Cdc7 (Cacdc7) homozygous null was not viable, we generated a C. albicans strain with a deleted C. albicans CDC7 (CaCDC7) allele and an expression-repressible allele. Surprisingly, cells of the strain grew as hyphae under the repressed conditions. The in vitro kinase assays confirmed that CaCdc7 (K232) and CaCdc7 (T437) are critical for catalytic and phosphoacceptor of activation activity, respectively. C. albicans cells formed hyphae when expressing either the catalytically inactive CaCdc7 (K232R) or the phosphoacceptor-deficient CaCdc7 (T437A). While CaCdc7 interacted with CaDbf4, cells of the strain in which CaCDC7 was repressed were not rescued by constitutively expressing C. albicans DBF4 or vice versa. We conclude that CaDBF4-dependent CaCDC7 is an essential gene suppressing the hyphal development.

Hypercohin K (1), the first polycyclic polyprenylated acylphloroglucinol possessing a unique spiro-fused cyclopropane ring, together with hyphenrone L (2) representing the most complex congeners featured with a hexacyclic core, was isolated from Hypericum cohaerens. The structure of 1 was elucidated by extensive MS and NMR spectroscopic data. The absolute configuration was defined by comparison of the experimental and calculated ECD. Compound 1 was evaluated for the inhibitory activities against acetylcholinesterase and four human tumor cell lines. (C) 2015 Elsevier Ltd. All rights reserved.

Simmons first introduced the concept of subliminal channel in 1984. After that, many researches of digital signature with subliminal channels have been proposed. But these schemes just allow a subliminal receiver to get one subliminal message unless he/she maintains more secret keys. In this paper, we will integrate the concepts of subliminal channel and access control to construct two digital signature schemes with subliminal channels. In the proposed schemes, the subliminal receivers can get different number of subliminal messages in accordance with users' positions. Moreover, each subliminal receiver only needs to keep one secret key.

Resistin is known as an adipocyte-specific hormone that can cause insulin resistance and decrease adipocyte differentiation. It can be regulated by transcriptional factors, but the possible role of forkhead transcription factor FOXO1 in regulating resistin gene expression is still unknown. Using 3T3 fibroblast and C3H10T1/2 and 3T3-L1 adipocytes, we found that transient overexpression of a non-phosphorylatable, constitutively active FOXO1, but not the wild type of FOXO1 or a DNA binding-deficient FOXO1, activated resistin promoter-directed luciferase expression. However, transient overexpression of a dominant-negative FOXO1 inactivated resistin promoter activity and reduced resistin mRNA expression. These observations indicate that the action of FOXO1 on resistin gene expression requires the activation of FOXO1 and that the effect of FOXO1 depends on the phosphorylation and dephosphorylation of FOXO1. The FOXO1 protein target sites on the resistin promoter were localized to the proximal -3545 to -787bp of 5'-flanking region of the resistin promoter. A chromatin immunoprecipitation assay also showed that FOXO1 bound the resistin promoter at nucleotide regions of -1539 to -1366bp and -1016 to -835bp, but not at the regions of -795 to -632bp. Results of this study suggest that FOXO1 transcription factor likely activates the expression of adipocyte resistin gene via direct association with the upstream resistin promoter.