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Tobacco is one of the important economic crops all over the world. Tobacco mosaic virus (TMV) seriously affects the yield and quality of tobacco leaves. The expression of TMV in tobacco leaves can be analyzed by detecting green fluorescence-related traits after inoculation with the infectious clone of TMV-GFP (Tobacco mosaic virus - green fluorescent protein). However, traditional methods for detecting TMV-GFP are time-consuming and laborious, and mostly require a lot of manual procedures. In this study, we develop a low-cost machine-vision-based phenotyping platform for the automatic evaluation of fluorescence-related traits in tobacco leaf based on digital camera and image processing. A dynamic monitoring experiment lasting 7 days was conducted to evaluate the efficiency of this platform using Nicotiana tabacum L. with a total of 14 samples, including the wild-type strain SR1 and 4 mutant lines generated by RNA interference technology. As a result, we found that green fluorescence area and brightness generally showed an increasing trend over time, and the trends were different among these SR1 and 4 mutant lines samples, where the maximum and minimum of green fluorescence area and brightness were mutant-4 and mutant-1 respectively. In conclusion, the platform can full-automatically extract fluorescence-related traits with the advantage of low-cost and high accuracy, which could be used in detecting dynamic changes of TMV-GFP in tobacco leaves.

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In order to study the thermodynamic effects of highway construction in plateau areas on frozen soil corridors, starting from the current practice of the frozen soil disease remediation project in plateau areas from 2017 to 2019, the mechanism of highway disease occurrence was discussed. The analysis was carried out, and the frozen soil was treated by the simulation test method. The field tests were conducted to investigate the actual use of the thermal insulation board subgrade and gravel subgrade of the diseased section. The temperature field distribution law of the frozen soil corridor project under periodic fluctuation of the boundary temperature was explored. The representative road section observation data was adopted to predict the temperature variation. Thermal characteristics of permafrost engineering corridors were analyzed. Taking the Golmud-Lhasa highway as the research object, the research results show that the construction method of the highway through the plateau directly affects the thermodynamic characteristics of the permafrost corridor project. Effective strategies have been obtained to control temperature changes, reduce roadbed damage, and enhance the thermal characteristics of permafrost engineering corridors.

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Additional file 2. Schedule of the study process

Introduction: Resistance ovary syndrome (ROS) is a disease characterized by hypergonadotropic amenorrhea but with normal ovarian reserve. Currently, its pathogenesis is still unclear and the treatment methods are complex. Nevertheless, there are evident negative effects of this disease on females’ physical and mental health such as gonadal dysplasia, infertility, anxiety, and depression. This article reports a case of successful ovulation induction and pregnancy with letrozole combined with HMG. This can provide clinical treatment guidelines for the disease. Patient concerns: The patient underwent several hormone replacement cycles and ovulation induction cycles. But the dominant follicles were not extracted even after using large doses of gonadotropin. Diagnosis: Resistant ovary syndrome; Primary infertility Interventions: Larger doses of letrozole combined with HMG were injected to stimulate ovulation and sensitize the ovaries during menstruation. This helped to examine the peripheral effects of letrozole in relation to gonadotropin. Outcomes: The patient displayed a dominant follicular growth and notable ovulation which resulted in a full-term pregnancy and successful delivery. Conclusions: The resistance ovary syndrome (ROS) can be treated and the findings from this case provides a possible treatment for ROS patients with infertility.

Thus far, all clinical trials evaluating the efficacy of embryo transfer strategies have selectively delayed the first frozen embryo transfer (FET) by at least 1 menstrual cycle. Nevertheless, this approach, which is based solely on clinical experience, may create unnecessary psychological stress on infertile patients who are anxious to conceive as soon as possible. This study aimed to investigate whether the time interval between oocyte retrieval and subsequent FET affects reproductive outcomes. We implemented a large retrospective cohort study in a single assisted reproductive technology (ART) unit at a university-based hospital, including 1540 autologous FET cycles performed in freeze-all cycles. The beginning of the FET was classified as either ‘cycle 1’ (performing FET within the first menstrual cycle) or ‘cycle ≥2’ (performing FET after one or more menstrual cycles). Live birth rate (LBR) was the primary outcome of our study. The mean interval for ‘cycle 1’ and ‘cycle ≥2’ FETs was 25.72 ± 5.10 days and 75.33 ± 24.85 days, respectively (P

This article presents an adaptive attitude tracking controller with external disturbances and unknown inertia parameters. The similar skew-symmetric structure is extended from the autonomous case to the non-autonomous case. The non-autonomous similar skew-symmetric is chosen as the desired structure of the closed loop system for attitude controller design. Based on this structure, a novel adaptive backstepping scheme is proposed to design the attitude controller by taking full advantage of the symmetry and the positive definiteness of the inertia matrix. The attitude tracking precision is enhanced by employing the linear parameterized form of the external disturbance torques. Simulation results demonstrate the effectiveness of the proposed attitude controller.

Autonomous On-orbit Service (OOS) is one of the key issues in aerospace technology nowadays. Since multi-robot system, which consists of several low-cost and simple robots, can work more efficiently and robustly than a single and complicated robot, we consider introducing multi-robot system into autonomous OOS. This paper focuses on the topic of task planning in multi-robot system, and takes advantage of expert system to solve this problem. We develop a new expert system called KICNP-S and a simulation platform called JavaRobot, which can simulate a group of intelligent robots performing OOS corporately. The simulation results show that expert system is competent in solving task planning problem, and we only need to modify the knowledge database when conditions of tasks change. Finally, simulation of a simplified OOS task is presented in which up to 13 robots repair a breakdown satellite.

This paper presents a mixed H 2 /H ∞ , state feedback control and its application to airborne dispenser pitch-axis autopilot design. Linear matrix inequality conditions that depend only on the vertices of polytope are given for existence of a stabilizing gain scheduled state feedback for linear parameter-varying system in convex polytopic domain, and the solution of the controller is given. The proposed method has the potential to yield less conservative results where the parameters involved can be measured or estimated online because some additional free variables are introduced to LMIs. The augmented system of airborne dispenser pitch-axis is modeled with mixed sensitivity method, applying the proposed method, good transient performance, good steady performance and good capability of disturbance rejection are readily achieved.

This paper reviews the problem of H 2 output feedback control for polytopic linear parameter-varying systems. Either plant or controller is not restricted to be time-invariant. By employing the definition of Quadratic H 2 Performance, sufficient conditions in terms of linear matrix inequalities are presented for the existence of the desired controller for the continuous time linear parameter-varying system. The conditions guarantee that the closed-loop system is quadratically stable and with a prescribed H 2 attenuation level, which is potentially less conservative than that of some previous results. A numerical example illustrates the effectiveness of the proposed approach.

This paper reviews the mixed H2/Hinfin filtering problem for linear time-invariant discrete-time systems with polytopic uncertainty. By introducing some additional free variables, a parameter dependent Lyapunov stability condition other than the quadratic stability condition is obtained in a polytopic domain, and then a much less conservative filter design approach is presented via solving some LMIs formulated with correspondence to polytopic vertices.

In intelligent transportation system (ITS), it is of great significance to share data among distributed and heterogeneous databases to realized system goal. In this paper a strategy of managing dynamic data information for multi-databases system in ITS under wide-area network (WAN) is proposed. Data directory is introduced to record data information of every database system, and is periodically updated by using alphabetical link for database structure set. Updating message is transferred peer-to-peer to avoid bottle neck at one system. Finally, the paper discusses how to detect the login and logout systems. A demo system based on the presented method is introduced to prove its validity.

Public transportation intelligent dispatching system (PTIDS) is the most important subsystem in advanced public transportation system (APTS). In order to realize intelligent dispatching, the dispatcher must determine the headway and dispatching form for a certain transit vehicle. As we know, the headway is relatively stable for some certain time period since the passenger flow changes according to regularity. However, the dispatching form must be determined according to the real time passenger flow. This paper proposes that the principle of automatic creating of a transit vehicle's dispatching form for the first time. Then, a new automatic creating method based on BP (backpropagation) neural network is introduced, which can make sure that the dispatching form can be created in a short time. The case study in Line 306 of Changchun, a metropolitan city of China, has proved that the method proposed In this paper is application.