Search

Supplemental material, sj-pdf-1-cph-10.1177_17151635211004975 for Physical assessment in pharmacy practice: Perspectives from pharmacists, nonpharmacist health care providers and the public by Christine Leong and Leila Soufi in Canadian Pharmacists Journal / Revue des Pharmaciens du Canada

Supplemental material, sj-pdf-2-cph-10.1177_17151635211004975 for Physical assessment in pharmacy practice: Perspectives from pharmacists, nonpharmacist health care providers and the public by Christine Leong and Leila Soufi in Canadian Pharmacists Journal / Revue des Pharmaciens du Canada

Supplemental material, sj-pdf-2-cph-10.1177_17151635211004975 for Physical assessment in pharmacy practice: Perspectives from pharmacists, nonpharmacist health care providers and the public by Christine Leong and Leila Soufi in Canadian Pharmacists Journal / Revue des Pharmaciens du Canada

Objectives: Pseudomonas aeruginosa is one of the main causes of nosocomial infections,\r\nespecially in immunocompromised patients in burn and intensive care units, who have\r\ninherited a number of drug resistance determinants via horizontal mutation. For this reason,\r\nour work has also been conducted to prove the genetic relationship between strains and to\r\ninvestigate the occurrence of genes conferring high levels of carbapenem and colistin\r\nresistance in Pseudomonas aeruginosa isolates from the Algerian hospital.\r\nMethodology: 27 colistin resistant Pseudomonas aeruginosa (CO-RPA) recovered from\r\npatients and hospital environment were analyzed using a combination of phenotypic and\r\nmolecular typing methods for the identification of plasmid mediated colistin resistance genes;\r\ncarbapenem resistant Pseudomonas aeruginosa (CRPA) isolates were tested for the presence\r\nof genes encoding β-lactamases, quinolones and β-lactamases.\r\nPCR-Ribotyping and PCR 16s rDNA were used to establish the phylogeny and clonal\r\nrelationship of strains.\r\nResults: Molecular Screening for mcr encoding genes showed that 02 tested isolates\r\nexamined contained mcr-1, one isolate of which expressed mcr-1 and OprD genes at the same\r\ntime. Interestingly, 62.95 % of the colistin-resistant strains were imipenem-resistant in this\r\nstudy. Actually, loss/mutation in OprD was founded in 29, 41% (05/17) of imipenem-resistant\r\nstrains. PCR- Ribotyping revealed an outbreak of clonal distribution of colistin and\r\ncarbapenem resistant Pseudomonas aeruginosa isolates.\r\nConclusion: The current study highlights the first potential spread of the mcr-1 gene in\r\nPseudomonas aeruginosa strains in the health care setting in Algeria and around the world\r\nthat appears to be linked to the uncontrolled use of antibiotics. Strict precautions will be\r\nexpected to prevent the further spread of these pathogens in hospital settings.

Here, we report the genomic features and the bioremediation potential of Halomonas desertis G11, a new halophilic species which uses crude oil as a carbon and energy source and displays intrinsic resistance to salt stress conditions (optimum growth at 10% NaCl). G11 genome (3.96 Mb) had a mean GC content of 57.82%, 3622 coding sequences, 480 subsystems and 64 RNA genes. Annotation predicted 38 genes involved in osmotic stress including the biosynthesis of osmoprotectants glycine-betaine, ectoine and osmoregulated periplasmic glucans. Genome analysis revealed also the versatility of the strain for emulsifying crude oil and metabolizing hydrocarbons. The ability of G11 to degrade crude oil components and to secrete a glycolipid biosurfactant with satisfying properties was experimentally confirmed and validated. Our results help to explain the exceptional capacity of G11 to survive at extreme desertic conditions, and highlight the metabolic features of this organism that has biotechnological and ecological potentialities.