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Indirect bioprinting for cell culture requires the use of several technologies and techniques which currently prevent many researchers not specialized in electrical engineering or materials science from accessing these new tools. In this paper, a printer and all necessary associated hardware was developed and tested for the purpose of seeding human induced Pluripotent Stem Cell (iPSC)-derived endothelial cells (iECs) onto all surfaces of a fibringelatin channel. Immature iECs were seeded onto all channel surfaces and completed differentiation along channel walls. All required tools and methods, including engineering drawing, printable files, code, and hand-tool templates, have been provided with sufficient clarity to enable full, open-source replication of all technique employed.

This study aimed to describe epidemiological aspects of schistosomiasis in Carne de Vaca, Goiana, on the coast of Pernambuco State, Brazil and analyze the spatial distribution of households' sanitary conditions and patterns of water contact. A parasitological survey was conducted between 2006 and 2007. An indicator was set to establish risk levels for schistosomiasis based on variables of water contact pattern, household characteristics and proximity to the foci of vector snails. The prevalence was 18.71 in men and 15.96 in women. The age groups most affected were 20-29 years among men and 10-19 among women. The most common parasite load was 1-99 eggs per gram of feces. The prevalence was 17.3 cases per 100 inhabitants and its spatial distribution was not homogeneous. The Bayesian analysis proved unsatisfactory. It is concluded that schistosomiasis is endemic in Carne de Vaca and the risk indicator, based on geoprocessing, proved to be satisfactory, highlighting areas where the problem is more acute.O estudo objetivou descrever aspectos epidemiológicos da esquistossomose em Carne de Vaca, litoral de Pernambuco, Brasil, e analisar a distribuição espacial das condições de esgotamento sanitário e padrão de contato com a água. Foi realizado um inquérito parasitológico entre 2006 e 2007, e criado indicador de risco para a esquistossomose com base em variáveis do padrão de água de contato, características do agregado familiar e da proximidade com focos de moluscos vetores. A prevalência em homens foi de 18,71, e 15,96 em mulheres. A faixa etária mais acometida em homens e mulheres foi de 20-29 e 10-19, respectivamente. A carga parasitária mais freqüente foi de 1-99 ovos por grama de fezes. A prevalência foi de 17,3 casos por 100 habitantes e sua distribuição espacial não foi homogênea. A estatística Bayesiana não se mostrou satisfatória. Conclui-se que a esquistossomose é endêmica em Carne de Vaca e que o indicador de risco com base em geoprocessamento revelou-se satisfatório, destacando as áreas onde o problema é mais relevante.

Background: Osteoporosis is one of the most important public health problems involving a high percentage of costs in the medical care system. Reliable diagnostic techniques for an early detection of bone deterioration and studies of factors that influence its development in menopausal women are crucial. The aim of the study was to determine the relationship between bone microarchitecture and anthropometry in climacteric women. Methods: Women were recruited at the Menopause Clinic, University Hospital of FMRP/USP, and submitted to anthropometry and to the evaluation of bone quality (Ultrasound Bone Profile Index, UBPI) and quantity (Amplitudedependent Speed of Sound, AD-SoS-) by phalangeal quantitative osteosonography (DBM Sonic BP). Descriptive analysis of the data was reported and a multiple linear regression was performed using the software SAS® 9.0. Results: 71 patients aged 58 ± 7 y were studied: 28% had BMI 18.5-24.9 kg/m², 35% BMI 24.9-29.9 and 37% BMI > 30. Mean AD-SoS was 2059 ± 79 m/s and mean UBPI was 0.67 ± 0.13. Considering AD-SoS the dependent variable, there was no statistically significant relationship between age (p = 0.20), BMI (p = 0.76), fat mass by bioelectrical impedance (p = 0.42) and by anthropometry (p = 0.95). The variables had very low effect on the UBPI when it was considered the dependent variable. Conclusions: The relation between bone microarchitecture and the anthropometry of the women studied shows that, the greater the bone quantity, the better the anthropometric parameters, without statistically significance. This work was a cross-sectional study on a small sample that needs to be validated in a prospective design. Introducción y objetivo: La osteoporosis es uno de los problemas más importantes de la Salud Pública e involucra un elevado porcentaje de los costos del Sistema de Salud. Es decisiva la aplicación de técnicas confiables de diagnóstico para la detección precoz del deterioro óseo y estudios de los factores que influencian su desarrollo en mujeres postmenopáusicas. El objetivo del estudio fue determinar la relación entre la micro arquitectura ósea y la antropometría de mujeres postmenopáusicas. Métodos: Se reclutaron mujeres, en el dispensario de Climaterio del Hospital Universitario (FMRP/USP), que fueron sometidas a antropometría y evaluación de la calidad ósea (Índice Ultrasonográfico del Perfil Óseo-UBPI) y de la cantidad ósea (Velocidad del Sonido dependiente de la Amplitud-Ad-SoS) por medio de la osteosonografía cuantitativa de falange (DBM Sonic BP). Se realizaron análisis descriptivos de los datos y regresión lineal múltiple utilizando el software SAS® 9.0. Resultados: Se estudiaron 71 pacientes con edad media de 58 ± 7 años: 28% tuvieron el IMC entre 18,5-24,9 kg/m², 35% entre 24,9-29,9 y 37% IMC arriba de 30. La media del Ad-SoS fue 2.059 ± 79 m/s y del UBPI fue 0,67 ± 0,13. Considerando el Ad-SoS como la variable dependiente, no hubo relación estadísticamente significativa entre la edad (p = 0,20), IMC (p = 0,76), masa grasa por la impedancia bioeléctrica (p = 0,42) y por la antropometría (p = 0,95). Las variables tuvieron un efecto muy bajo en el UBPI cuando este fue considerado la variable dependiente. Conclusión: La relación entre la micro arquitectura ósea y la antropometría de las mujeres estudiadas mostró que cuanto mayor es la cantidad ósea, mejores son los parámetros antropométricos sin importancia estadística. Este trabajo fue un estudio transversal de una muestra pequeña, por lo cual necesita ser validado en un diseño prospectivo.

Made available in DSpace on 2013-08-12T19:09:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-03-01 Made available in DSpace on 2013-09-30T19:20:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-03-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:33:12Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T15:33:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-03-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Background: Osteoporosis is one of the most important public health problems involving a high percentage of costs in the medical care system. Reliable diagnostic techniques for an early detection of bone deterioration and studies of factors that influence its development in menopausal women are crucial. The aim of the study was to determine the relationship between bone microarchitecture and anthropometry in climacteric women.Methods: Women were recruited at the Menopause Clinic, University Hospital of FMRP/USP, and submitted to anthropometry and to the evaluation of bone quality (Ultrasound Bone Profile Index, UBPI) and quantity (Amplitude-dependent Speed of Sound, AD-SoS-) by phalangeal quantitative osteosonography (DBM Sonic BP). Descriptive analysis of the data was reported and a multiple linear regression was performed using the software SASS (R) 9.0.Results: 71 patients aged 58 +/- 7 y were studied: 28% had BMI 18.5-24.9 kg/m(2), 35% BMI 24.9-29.9 and 37% BMI > 30. Mean AD-SoS was 2059 +/- 79 m/s and mean UBPI was 0.67 +/- 0.13. Considering AD-SoS the dependent variable, there was no statistically significant relationship between age (p = 0.20), BMI (p = 0.76), fat mass by bioelectrical impedance (p = 0.42) and by anthropometry (p = 0.95). The variables had very low effect on the UBPI when it was considered the dependent variable.Conclusions: The relation between bone microarchitecture and the anthropometry of the women studied shows that, the greater the bone quantity, the better the anthropometric parameters, without statistically significance. This work was a cross-sectional study on a small sample that needs to be validated in a prospective design. (Nutr Hosp. 2012;27:612-616) DOI: 10.3305/nh.2012.27.2.5625 Univ São Paulo FMRP USP, Med Sch Ribeirao Preto, Dept Internal Med Clin Nutr, BR-14049900 São Paulo, Brazil State Univ São Paulo UNESP, Sch Pharmaceut Sci, Dept Food & Nutr, São Paulo, Brazil Univ São Paulo FMRP USP, Med Sch Ribeirao Preto, Dept Gynecol & Obstet, BR-14049900 São Paulo, Brazil State Univ São Paulo UNESP, Sch Pharmaceut Sci, Dept Food & Nutr, São Paulo, Brazil FAPESP: 05/53935-0

Background & aim: Many disease outbreaks of food origin are caused by foods prepared in Food Service and Nutrition Units of hospitals, affecting hospitalized patients who, in most cases, are immunocompromised and therefore at a higher risk of severe worsening of their clinical status. The aim of this study was to determine the variations in temperature and the time-temperature factor of hospital diets. Methods: The time and temperature for the preparation of 4 diets of modified consistency were determined on 5 nonconsecutive days in a hospital Diet and Nutrition Unit at the end of preparation and during the maintenance period, portioning and distribution at 3 sites, i.e., the first, the middle and the last to receive the diets. Results and discussion: All foods reached an adequate temperature at the end of cooking, but temperature varied significantly from the maintenance period to the final distribution, characterizing critical periods for microorganism proliferation. During holding, temperatures that presented a risk were reached by 16.7% of the meats and 59% of the salads of the general diet, by 16.7% of the garnishes in the bland diet and by 20% of the meats and garnishes in the viscous diet. The same occurred at the end of distribution for 100% of the hot samples and of the salads and for 61% of the desserts. None of the preparations remained at risk temperature for a time exceeding that established by law. Conclusion: The exposure to inadequate temperature did not last long enough to pose risks to the patient. Antecedentes y objetivos: muchas epidemias de origen alimentario están causadas por alimentos preparados en las unidades de alimentación y nutrición de los hospitales y afectan a pacientes hospitalizados que, en su mayoría, están inmunodeprimidos y presentan, por lo tanto, un mayor riesgo de empeoramiento grave de su estado clínico. El objetivo de este estudio fue determinar las variaciones en la temperatura y el factor tiempo-temperatura en las dietas de los hospitales. Métodos: se determinó el tiempo y la temperatura de preparación de 4 dietas de consistencia modificada durante 5 días consecutivos en una Unidad de alimentación y nutrición, al final de la preparación y durante el periodo de mantenimiento, racionamiento y distribución en 3 sitios, es decir, el primer sitio en recibir la dieta, el intermedio y el último. Resultados y discusión: todos los alimentos alcanzaron una temperatura adecuada al final de su preparación, pero la temperatura varió significativamente desde su periodo de mantenimiento hasta su distribución final, caracterizando periodos críticos para la proliferación de microorganismos. Durante su almacenamiento, las temperaturas que representaron un riesgo se dieron en el 16,7% de las carnes y el 59% de las ensaladas de la dieta general, en el 16,7% de las guarniciones de la dieta blanda y en el 20% de las carnes y las guarniciones de la dieta viscosa. Lo mismo ocurrió al final de la distribución en el 100% de las muestras calientes y de las ensaladas y en el 61% de los postres. Ninguna de las preparaciones permaneció a una temperatura de riesgo durante un tiempo que excediese el tiempo establecido por ley. Conclusión: La exposición a una temperatura inadecuada no fue lo suficientemente prolongada para presentar un riesgo para el paciente.

Background & aim: Many disease outbreaks of food origin are caused by foods prepared in Food Service and Nutrition Units of hospitals, affecting hospitalized patients who, in most cases, are immunocompromised and therefore at a higher risk of severe worsening of their clinical status. The aim of this study was to determine the variations in temperature and the time-temperature factor of hospital diets. Methods: The time and temperature for the preparation of 4 diets of modified consistency were determined on 5 nonconsecutive days in a hospital Diet and Nutrition Unit at the end of preparation and during the maintenance period, portioning and distribution at 3 sites, i.e., the first, the middle and the last to receive the diets. Results and discussion: All foods reached an adequate temperature at the end of cooking, but temperature varied significantly from the maintenance period to the final distribution, characterizing critical periods for microorganism proliferation. During holding, temperatures that presented a risk were reached by 16.7% of the meats and 59% of the salads of the general diet, by 16.7% of the garnishes in the bland diet and by 20% of the meats and garnishes in the viscous diet. The same occurred at the end of distribution for 100% of the hot samples and of the salads and for 61% of the desserts. None of the preparations remained at risk temperature for a time exceeding that established by law. Conclusion: The exposure to inadequate temperature did not last long enough to pose risks to the patient.