Search

Female reproductive cycle, also known as menstrual cycle or estrous cycle in primate or non-primate mammals, respectively, dominates the reproductive processes in non-pregnant state. However, in addition to reproductive tissues, reproductive cycle could also perform global regulation because the receptors of two major female hormones fluctuating throughout the cycle, estrogen and progesterone, are widely distributed. Therefore, a multi-tissue gene expression landscape is in continuous demand for better understanding the systemic changes during the reproductive cycle but remains largely undefined. Here we delineated a transcriptomic landscape covering 15 tissues of C57BL/6J female mice in two phases of estrous cycle, estrus and diestrus, by RNA-sequencing. Then, a number of genes, pathways, and transcription factors involved in the estrous cycle were revealed. We found the estrous cycle could widely regulate the neuro-functions, immuno-functions, blood coagulation and so on. And behind the transcriptomic alteration between estrus and diestrus, 13 transcription factors may play important roles. Next, bioinformatics modeling with 1,263 manually curated gene signatures of various physiological and pathophysiological states systematically characterized the beneficial/deleterious effects brought by estrus/diestrus on individual tissues. We revealed that the estrous cycle has a significant effect on cardiovascular system (aorta, heart, vein), in which the anti-hypertensive pattern in aorta induced by estrus is one of the most striking findings. Inspired by this point, we validated that two hypotensive drugs, felodipine and acebutolol, could exhibit significantly enhanced efficacy in estrus than diestrus by mouse and rat experiments. Together, this study provides a valuable data resource for investigating reproductive cycle from a transcriptomic perspective, and presents models and clues for investigating precision medicine associated with reproductive cycle.

Myocardial infarction (MI) is a devastating disease with high morbidity and mortality caused by the irreversible loss of functional cardiomyocytes and heart failure (HF) due to the restricted blood supply. Mesenchymal stem cells (MSCs) have been emerging as lead candidates to treat MI and subsequent HF mainly through secreting multitudinous factors of which exosomes act as the most effective constituent to boost the repair of heart function through carrying noncoding RNAs and proteins. Given the advantages of higher stability in the circulation, lower toxicity, and controllable transplantation dosage, exosomes have been described as a wonderful and promising cell-free treatment method in cardiovascular disease. Nowadays, MSC-derived exosomes have been proposed as a promising therapeutic approach to improve cardiac function and reverse heart remodeling. However, exosomes’ lack of modification cannot result in desired therapeutic effect. Hence, optimized exosomes can be developed via various engineering methods such as pharmacological compound preconditioned MSCs, genetically modified MSCs, or miRNA-loaded exosomes and peptide tagged exosomes to improve the targeting and therapeutic effects of exosomes. The biological characteristics, therapeutic potential, and optimizing strategy of exosomes will be described in our review.

The project adopted component ice-storage model ice storage system, and airside took low temperature supply air at °C. Compared with conventional air-conditioning systems,ice storage air conditioning system adds investment about 99.5 million Yuan, annual operation cost saves about 22 million Yuan, additional investment recovery period is 4.52 years, investment would achieve 230.56 million Yuan in entire life cycle of air conditioning.

View of the current issues facing the global energy crisis, energy conservation has been widely attention. The energy consumption of air conditioning engineering are huge. Because the energy used for dehumidification in air conditioning energy consumed by an alarming proportion, so this paper analyzes the domestic and foreign research in recent years, several large and mature dehumidification mode, especially for liquid desiccant technology, in detail experimental study. By changing the inlet air temperature, humidity, imports solution temperature, concentration, air and solution flow rate, etc., to observe the right solution dehumidifying performance. Came to the conclusion liquid desiccant air conditioning system has good prospects for development.

The relationship between the nitrogenous biological oxygen demand (NBOD) and the oxidizable nitrogenous pollutants in the Lee River has been determined to be dependent upon the hydrological conditions. When there is no water discharge (NWD) from the upper reservoir, this relationship is quite clear and definite, but under water discharge (WD) conditions, this relationship is ill-defined. Mean ratios of NBOD5 to BOD5 are 0.620 for the NWD case and 0.379 for the WD case, with maximum ratios of 0.944 and 0.643, respectively. Differences between these two cases are caused mainly by the variations of several factors such as concentration of oxidizable nitrogen compounds, an abundance of nitrifying organisms, pH and velocity and depth of water. The hydrological conditions play an important role in these variations.