1. Restriction of measles virus RNA synthesis by a mouse host cell. Trans-complementation by polymerase components or human cellular factor(s)
- Author
-
S Tigaud I Schneider H Buchholz Cj Yanagi Y Gerlier D., Vincent, Virologie et pathogenèse virale (VPV), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Laboratoire de Cytogénétique Moléculaire, Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Institut für Molekularbiologie, Universität Zürich [Zürich] = University of Zurich (UZH), Department of Virology, Faculty of Medicine, Kyushu University, and This work was performed with financial support from the Commission of European Communities (RTD programme 'Quality of Life and Management of Living Resources' [QLK2-CT2001-01225]) and from the Ministère de l'Education Nationale de la Recherche et de la Technologie (PRFMMIP). The content of this publication does not necessarily reflect the views of the Commission of European Communities and in no way anticipates the Commission's future policy in this area.
- Subjects
Glycoproteins/genetics/*metabolism ,Genetic Complementation Test ,Viral/*biosynthesis/genetics ,Immunoglobulins/genetics/*metabolism ,Epithelial Cells/virology ,Hybrid Cells/virology ,DNA-Directed RNA Polymerases/genetics/*metabolism ,Viral Proteins/genetics/metabolism ,Research Support ,Virus Replication ,Cell Line ,CD/genetics/*metabolism ,Measles virus/genetics/pathogenicity/*physiology ,Mice ,Hela Cells/virology ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Animals ,Humans ,RNA ,Antigens ,Non-U.S. Gov't ,OCIS 000.1430 ,CD46 ,Membrane Glycoproteins/genetics/*metabolism ,Intestines/cytology - Abstract
The mouse epithelial MODE-K cell line expressing human CD46 or CD150 cellular receptors was found to be nonpermissive for measles virus (MV) replication. The virus binding and membrane fusion steps were unimpaired, but only very limited amounts of virus protein and RNA synthesized were detected after the infection. In a minigenome chloramphenicol acetyltransferase assay, MODE-K cells were as able as the permissive HeLa cells in supporting MV polymerase activity. The restriction phenotype of MODE-K cells could be alleviated by providing, in trans, either N-P-L or N-P functional protein complexes but not by P-L complexes or individual N, P, and L proteins. Several human x mouse (HeLa x MODE-K) somatic hybrid clones expressing human CD46 were isolated and found to be either nonpermissive or permissive according to their human chromosomal contents. The MV-restricted phenotype exhibited by the MODE-K cell line suggests that a cellular factor(s) can control MV transcription, possibly by stabilizing the incoming virus polymerase templates.
- Published
- 2006