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42 results on '"Vachier, Isabelle"'

1. Empty outcome table

Author

Suehs, Carey, Bourdin, Arnaud, Nicolas, Audrey, Martinez, Aurelie, Molinari, Nicolas, and Vachier, Isabelle

Published
2022
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2. CLIPPCAIR

Author

Bommart, Sébastien, Suehs, Carey, Marty-Ané, Charles, Vachier, Isabelle, Charriot, Jeremy, Hrieche, Kheira, Solovei, Laurence, Hayot, Maurice, Gouzi, Fares, Serre, Isabelle, Molinari, Nicolas, and Bourdin, Arnaud

Subjects
Medicine and Health Sciences
Abstract

The primary objective of the CLIPPCAIR study is to construct and validate a new algorithm for predicting post-operative forced expiratory volume in 1 second (FEV1) values for lung resection candidates; this new model will be based on data derived from a thoracic CT scan with injection of contrast media. This registration includes information contained in the eCRF.

Published
2022
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4. CortiCORONA

Author

Boissin, Clement, Charriot, Jeremy, Bourdin, Arnaud, Vachier, Isabelle, Suehs, Carey, and Molinari, Nicolas

Subjects
body regions, viruses, fungi, Medicine and Health Sciences, skin and connective tissue diseases, respiratory tract diseases
Abstract

Determination of biomarkers to predict the response to treatment with dexamethasone for Sars-Cov(2 / COVID-19 pneumonia

Published
2022
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5. CLIPPCAIR

Author

Bommart, Sébastien, Suehs, Carey, Marty-Ané, Charles, Vachier, Isabelle, Charriot, Jeremy, Hrieche, Kheira, Solovei, Laurence, Hayot, Maurice, Gouzi, Fares, Serre, Isabelle, Molinari, Nicolas, and Bourdin, Arnaud

Subjects
Medicine and Health Sciences, lung resection, fev1, lung function, spirometry
Abstract

The primary objective of the CLIPPCAIR study is to construct and validate a new algorithm for predicting post-operative forced expiratory volume in 1 second (FEV1) values for lung resection candidates; this new model will be based on data derived from a thoracic CT scan with injection of contrast media.

Published
2022
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6. CortiCORONA

Author

Boissin, Clement, Charriot, Jeremy, Bourdin, Arnaud, Vachier, Isabelle, Suehs, Carey, and Molinari, Nicolas

Subjects
body regions, viruses, fungi, Medicine and Health Sciences, corticosteroid response, COVID-19, SARS-COV-2, skin and connective tissue diseases, respiratory tract diseases
Abstract

Determination of biomarkers to predict the response to treatment with dexamethasone for Sars-Cov(2 / COVID-19 pneumonia

Published
2022
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8. eo-Delphi

Author

Suehs, Carey, Vachier, Isabelle, Molinari, Nicolas, and Bourdin, Arnaud

Abstract

eo-Delphi aims to create consensus for a core set of outcomes for the eo-Drive protocol using a modified Delphi method.

Published
2022
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10. Protocol

Author

Suehs, Carey, Boissin, Clement, Charriot, Jeremy, Bourdin, Arnaud, Vachier, Isabelle, and Molinari, Nicolas

Published
2022
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12. Study documents

Author

Suehs, Carey, Boissin, Clement, Charriot, Jeremy, Bourdin, Arnaud, Vachier, Isabelle, and Molinari, Nicolas

Published
2022
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13. The eo-Delphi protocol

Author

Suehs, Carey, Molinari, Nicolas, Vachier, Isabelle, and Bourdin, Arnaud

Subjects
modified delphi process, COPD, eo-Delphi, eosinophils, protocol, delphi process, corticosteroids
Published
2022
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14. CortiCORONA

Author

Boissin, Clement, Charriot, Jeremy, Bourdin, Arnaud, Vachier, Isabelle, Suehs, Carey, and Molinari, Nicolas

Subjects
body regions, viruses, fungi, skin and connective tissue diseases, respiratory tract diseases
Abstract

Determination of biomarkers to predict the response to treatment with dexamethasone for Sars-Cov(2 / COVID-19 pneumonia

Published
2022
Full Text
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15. CortiCORONA

Author

Boissin, Clement, Charriot, Jeremy, Bourdin, Arnaud, Vachier, Isabelle, Suehs, Carey, and Molinari, Nicolas

Subjects
body regions, viruses, fungi, Medicine and Health Sciences, CORTICOSTEROIDS, COVID-19, skin and connective tissue diseases, respiratory tract diseases
Abstract

Determination of biomarkers to predict the response to treatment with dexamethasone for Sars-Cov(2 / COVID-19 pneumonia

Published
2022
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17. Protocol

Author

Suehs, Carey, Boissin, Clement, Charriot, Jeremy, Bourdin, Arnaud, Vachier, Isabelle, and Molinari, Nicolas

Published
2022
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19. COPD-CARhE

Author

Charriot, Jeremy, Suehs, Carey, Vachier, Isabelle, Gamez, Anne-Sophie, Jean, Romain, Berger, Patrick, Zysman, Maeva, Guilleminault, Laurent, Didier, Alain, Brouquières, Danielle, Guibert, Nicolas, Marchandise, Sandrine, Molinari, Nicolas, and Bourdin, Arnaud

Published
2022
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20. PROTOACCEPT2

Author

Barrionuevo, Christian, Vachier, Isabelle, Suehs, Carey, Molinari, Nicolas, and Bourdin, Arnaud

Published
2022
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22. eo-Drive

Author

Bourdin, Arnaud, Suehs, Carey, Vachier, Isabelle, Molinari, Nicolas, Chanez, Pascal, Roche, Nicolas, Burgel, Pierre-Régis, Chinet, Thierry, Demoule, Alexandre, Similowski, Thomas, Morelot, Capucine, Londner, Cecile, Chenivesse, Cecile, Le Rouzic, Olivier, Bautin, Nathalie, Perez, Thierry, Raherison, Chantal, Molimard, Mathieu, Berger, Patrick, Girodet, Pierre, Yali, Matthieu, Devouassoux, Gilles, Didier, Alain, Noel-Savina, Elise, Couturaud, Francis, DESLEE, Chabaud, François, Chaouat, Ari, Guillaumot, Anne, Zysman, Maeva, Devillier, Philippe, Kessler, Romain, Just, Nicolas, Gamez, Anne-Sophie, and Boyer, Laurent

Subjects
exacerbation, biomarker driven, COPD, eosinophilia, corticosteroids, hospitalization
Published
2022
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23. CLIPPCAIR

Author

Bommart, Sébastien, Suehs, Carey, Marty-Ané, Charles, Vachier, Isabelle, Charriot, Jeremy, Hrieche, Kheira, Solovei, Laurence, Hayot, Maurice, Gouzi, Fares, Serre, Isabelle, Molinari, Nicolas, and Bourdin, Arnaud

Published
2022
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24. Supplementary file on the standard rheometry data, microrheology complementary data, and mucus composition data from Mucus from human bronchial epithelial cultures: rheology and adhesion across length scales

Author

Jory, Myriam, Donnarumma, Dario, Blanc, Christophe, Bellouma, Karim, Fort, Aurélie, Vachier, Isabelle, Casanellas, Laura, Bourdin, Arnaud, and Massiera, Gladys

Abstract

The supplementary files contains detailed data mentionned in the main text on the standard rheometry results. The experiments limitations, the yield stress measurements and the non-linear (LAOS) measurements are described in details, as well as the response variability depending on the culture day. The micromanipulations performed with the optical tweezers are also detailed: The response dependance upon the probe size and materials is detailed, as well as the elastic moduli close to the interface. Fially, the mucus composition / rheology correlation can be evaluated on provided Figures, corresponding to the discussion in the main text.

Published
2022
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25. Les organoïdes pulmonaires

Author

Bourguignon, Chloé, Vernisse, Charlotte, Mianné, Joffrey, Fieldes, Mathieu, Ahmed, Engi, Petit, Aurélie, Vachier, Isabelle, Bertrand, Thierry Lavabre, Assou, Said, Bourdin, Arnaud, De Vos, John, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), and Centre Hospitalier Universitaire de Nîmes (CHU Nîmes)

Subjects
Gene Editing, Tissue Scaffolds, Pulmonary Gas Exchange, [SDV]Life Sciences [q-bio], Cell Culture Techniques, Bioengineering, Respiratory Mucosa, Carbon Dioxide, Models, Biological, Organoids, Oxygen, Alveolar Epithelial Cells, Animals, Humans, Lung, Cells, Cultured
Abstract

As burden of chronic respiratory diseases is constantly increasing, improving in vitro lung models is essential in order to reproduce as closely as possible the complex pulmonary architecture, responsible for oxygen uptake and carbon dioxide clearance. The study of diseases that affect the respiratory system has benefited from in vitro reconstructions of the respiratory epithelium with inserts in air/liquid interface (2D) or in organoids able to mimic up to the arborescence of the respiratory tree (3D). Recent development in the fields of pluripotent stem cells-derived organoids and genome editing technologies has provided new insights to better understand pulmonary diseases and to find new therapeutic perspectives.Les organoïdes pulmonaires.L’impact en santé publique des pathologies respiratoires chroniques ne cesse de croître. Dans ce contexte, il paraît indispensable d’améliorer les modèles d’études du poumon afin de reproduire au plus proche l’architecture pulmonaire complexe, garante des fonctions d’oxygénation et d’épuration du gaz carbonique. Les connaissances actuelles en physiopathologie respiratoire résultent en partie des études de modèles de reconstitution d’épithélium bronchique in vitro à partir de cellules primaires, en deux dimensions sur des inserts, ou en trois dimensions, en organoïdes mimant jusqu’à l’arborescence pulmonaire. Le développement de ces modèles in vitro a connu un nouvel essor grâce aux organoïdes pulmonaires issus de cellules souches pluripotentes et la démocratisation des outils d’édition du génome. Ces apports technologiques récents offrent de nouvelles perspectives en matière de thérapeutiques ou de compréhension physiopathologique et pourraient, dans le futur, ouvrir les portes de la médecine régénératrice pulmonaire.

Published
2020

26. Mucus rheology and cilia beating coordination

Author

JORY, Myriam, Blanc, Christophe, Bellouma, Karim, Fort, Aurélie, Vachier, Isabelle, Bourdin, Arnaud, CASANELLAS VILAGELIU, Laura, MASSIERA, Gladys, Laboratoire Charles Coulomb (L2C), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Matière Molle, Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service d'allergologie et de pneumologie [Hôpital Arnaud de Villeneuve], Centre Hospitalier Universitaire de Montpellier (CHU Montpellier ), GDR CellTiss, and Projet de thèse: 'Approche biophysique de la fonction mucociliaire: rhéologie du mucus et coordination ciliaireCollaboration avec le CHU de Montpellier

Subjects
Mucus, Human bronchial epithelium, Optical Tweezers, Coordination, [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], Cilia, respiratory system, Rheology, Videomicroscopy
Abstract

International audience; The bronchial epithelium mucociliary function ensuring the lung continuous clearance relies on mucus rheological properties and cilia beating coordination. We focus on two points: first, the biochemical and physical parameters affecting the mucus viscoelastic properties; secondly, the cilia coordination and its coupling with the mucus.We performed rheological experiments on mucus extracted from Air Liquid Interface cultures of bronchial epithelium reconstituted from bronchial biopsies. We combine standard macro-rheology to micro-rheology performed with optical tweezers, to quantify the mucus flowing behaviour at two scales: the mucus layer thickness and the cilia or bacteria scale. Macro- and micro-rheology give different results: an elastic plateau (1-2 Pa) at the macro-scale; a viscous flow (10-3-10-2 Pa) at the micro-scale but with an important adhesion and elasticity at the interfaces. We also perform original experiments using optical tweezers directly on the epithelium, to access the viscoelastic response in the various mucus layers. We obtain an increased elasticity in the vicinity to the epithelium.Finally, the study of the physical mucus properties coupled with the specific methodology we developed to quantify cilia activity, coordination and mucus velocity field, should help to evaluate the efficiency of the mucociliary function and to understand the mechanisms of clearance.

Published
2018

27. Inferring data on cilia spatiotemporal coordination through bronchial epithelium imaging

Author

JORY, Myriam, Fort, Aurélie, CASANELLAS VILAGELIU, Laura, Vachier, Isabelle, Bourdin, Arnaud, MASSIERA, Gladys, Laboratoire Charles Coulomb (L2C), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Matière Molle, Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Service d'allergologie et de pneumologie [Hôpital Arnaud de Villeneuve], Centre Hospitalier Universitaire de Montpellier (CHU Montpellier ), EMBO, Projet de thèse: 'Approche biophysique de la fonction mucociliaire: rhéologie du mucus et coordination ciliaireCollaboration avec le CHU de Montpellier, WE-Heraeus, and Projet de thèse: 'Approche biophysique de la fonction mucociliaire: rhéologie du mucus et coordination ciliaire'Collaboration avec le CHU de Montpellier

Subjects
Human bronchial epithelium, Image processing, Coordination, [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], Cilia, respiratory system, Videomicroscopy
Abstract

International audience; The mucociliary function of the bronchial epithelium ensures the continuous clearance of the respiratory system. This function relies on two main elements: mucus properties and cilia beating coordination. We work on high speed optical microscopy videos of Human bronchial epithelium from ALI (Air-liquid interface) cultures. We present here a technique based on the tracking of each cilium and on the spatiotemporal analysis of the obtained trajectories. To relate each cilium to its corresponding cell, a clustering method is used. The spatiotemporal coordination can than be quantified both, inside and in-between ciliated cells. In addition the physical parameters can be mapped to observe the heterogeneity of the cilia activity and compare different pathologies.

Published
2018

28. Macro and Micro rheological characterization of Human bronchial mucus

Author

JORY, Myriam, Bellouma, Karim, Blanc, Christophe, Fort, Aurélie, Vernisse, Charlotte, CASANELLAS VILAGELIU, Laura, Vachier, Isabelle, Bourdin, Arnaud, MASSIERA, Gladys, Laboratoire Charles Coulomb (L2C), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Matière Molle, Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Service d'allergologie et de pneumologie [Hôpital Arnaud de Villeneuve], Centre Hospitalier Universitaire de Montpellier (CHU Montpellier ), EMBO, and Projet de thèse: 'Approche biophysique de la fonction mucociliaire: rhéologie du mucus et coordination ciliaireCollaboration avec le CHU de Montpellier

Subjects
Mucus, Human bronchial epithelium, fluids and secretions, Optical Tweezers, [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], Micro rheology, Adhesion, respiratory system, Macro Rheology, respiratory tract diseases
Abstract

International audience; The mucociliary function of the bronchial epithelium ensures the continuous clearance of the respiratory system. This function relies on two main elements: mucus and cilia beating. We perform here a rheological characterization of mucus samples extracted from ALI (Air-liquid interface) cultures of bronchial epithelium. Our approach combines macro- and micro-rheology techniques with the aim of quantifying the mucus viscoelastic properties at different length scales (from the size of bronchial cilia up to the scale on which mucus is transported). In addition, this methodology allows us to compare the rheological behavior of mucus samples corresponding to different pathologies.

Published
2018

29. Generation of the induced pluripotent stem cell line UHOMi001-A from a patient with mutations in CCDC40 gene causing Primary Ciliary Dyskinesia (PCD)

Author

Ahmed, Engi, Sansac, Caroline, Fieldes, Mathieu, Bergougnoux, Anne, Bourguignon , Chloé, Mianne, Joffrey, Arnould, Cécile, Vachier, Isabelle, Assou, Said, Bourdin, Arnaud, John, De Vos, MORNET, Dominique, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), and Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], Adult, lcsh:Biology (General), [SDV]Life Sciences [q-bio], Induced Pluripotent Stem Cells, Mutation, Humans, Proteins, Female, lcsh:QH301-705.5, ComputingMilieux_MISCELLANEOUS, Ciliary Motility Disorders
Abstract

Primary Ciliary Dyskinesia (PCD) is a rare heterogeneous genetic disorder affecting motile cilia structure and function leading to lung disease. We generated induced pluripotent stem cells (iPSCs) from dermal fibroblasts of a female PCD patient carrying disease-causing variants in the CCDC40 gene. Reprogramming was performed with the human OSKM transcription factors using the Sendai-virus delivery system. The resulting transgene free iPSCs had normal karyotype, expressed pluripotency markers, could differentiate into the three germ layers in vivo and retained the disease-causing CCDC40 mutations. This iPSC line could be useful to model PCD disease and test gene therapy strategies.Resource TableUnlabelled TableUnique stem cell line identifierUHOMi001-AAlternative name(s) of stem cell lineiPCD02.30InstitutionInstitute for Regenerative Medicine & Biotherapy (IRMB), Montpellier, FRANCEContact information of distributorJohn De Vos john.devos@inserm.frType of cell lineiPSCOriginhumanAdditional origin infoAge: 34Sex: FemaleCell SourceDermal fibroblastsClonalityClonalMethod of reprogramminghOCT4, hSOX2, hC-MYC, hKLF4 (CytoTune™-iPS 2.0 Sendai Reprogramming Kit - Invitrogen, Thermo Fisher Scientific Inc.)Genetic ModificationYESType of ModificationSpontaneous mutationAssociated diseasePrimary Ciliary DyskinesiaGene/locusCompound Heterozygous mutations the Coiled-Coil Domain Containing 40 Gene (CCDC40): c.1116_1117delCT (Exon 7) and c.3180 + 1G > A (Intron 19)Method of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock date2018-02-12Cell line repository/bankN/AEthical approvalThe study was approved by the regional scientific ethical committee (CPP Sud Med IV) under the number ID-RCB: 2013-A00892–43/CILIPS, Promoter University Hospital Of Montpellier) and informed consent was obtained from the patient.

Published
2018

30. Biophysical approach of the mucociliary function: Mucus rheology and beating coordination

Author

JORY, Myriam, Bellouma, Karim, Blanc, Christophe, Fort, Aurélie, Vernisse, Charlotte, CASANELLAS VILAGELIU, Laura, Vachier, Isabelle, Bourdin, Arnaud, MASSIERA, Gladys, Laboratoire Charles Coulomb (L2C), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Matière Molle, Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Service d'allergologie et de pneumologie [Hôpital Arnaud de Villeneuve], Centre Hospitalier Universitaire de Montpellier (CHU Montpellier ), COST MP1305 Flowing Mater, Projet de thèseCollaboration avec le CHU de Montpellier, EPIGENMED and Labex NUMEV, and Projet de thèse: 'Approche biophysique de la fonction mucociliaire: rhéologie du mucus et coordination ciliaireCollaboration avec le CHU de Montpellier

Subjects
Micro-rheology, Optical Tweezers, [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], respiratory system, Mucus, Human bronchial epithelium, Image processing, Coordination, Micro rheology, Adhesion, Cilia, Rheology, Videomicroscopy, Macro Rheology
Abstract

National audience; The bronchial epithelium mucociliary function ensuring the lung continuous clearance relies on mucus rheological properties and cilia beating coordination. Our biophysical approach aims at understanding two points. First, the biochemical and physical parameters affecting the mucus viscoelastic properties and evaluating its use as a marker for the diagnosis of respiratory diseases. Secondly the cilia coordination and its coupling with the mucus.We performed rheological experiments on mucus extracted from Air Liquid Interface (ALI) cultures of bronchial epithelium reconstituted from bronchial biopsies. Our approach consists in combining standard macro-rheology to micro-rheology performed with optical tweezers, in order to quantify the mucus flowing behavior at different scales, from the cilia up to the mucus layer length scales.Macro- and micro-rheology give different results. At the macro-scale, we obtain an elastic plateau (1 to 2 Pa), a shear-thinning behaviour and a very low yield stress (0.05-0.2 Pa). While at the micro-scale, we obtain a viscous flow, with a very low viscoelastic modulus (10-3-10-2 Pa). Nevertheless, by applying local forces at the interfaces, we observe an important adhesion and elasticity. Mucus adhesion should be taken into account to understand the differences between these different length scales and more generally to characterize the mucus properties. We also perform original experiments using optical tweezers directly on the epithelium, to access the viscoelastic response in the various mucus layers. We obtain an increased elasticity in the vicinity to the epithelium. Moreover, comparison of physical properties of mucus from the culture and directly extracted from patient lungs, provides an additional validation of the ALI model.Finally, the specific methodology we developed to quantify cilia activity and coordination, allows us to map, the cilia spatial distribution, the beating frequency and orientation of each cilium, and their standard deviation within a cilia cluster (cell). Local quantification of the cilia activity coupled to the measurement of the mucus velocity field, should help to evaluate the efficiency of the mucociliary function and to understand the mechanisms of clearance.

Published
2018

31. Biophysical approach of the mucociliary function: Mucus rheology and beating coordination

Author

JORY, Myriam, DONNARUMMA, Dario, Blanc, Christophe, CASANELLAS VILAGELIU, Laura, Fort, Aurélie, Vachier, Isabelle, Bourdin, Arnaud, MASSIERA, Gladys, Laboratoire Charles Coulomb (L2C), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Matière Molle, Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Service d'allergologie et de pneumologie [Hôpital Arnaud de Villeneuve], Centre Hospitalier Universitaire de Montpellier (CHU Montpellier ), and Projet de thèseCollaboration avec le CHU de Montpellier

Subjects
fluids and secretions, [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], cardiovascular system, technology, industry, and agriculture, lipids (amino acids, peptides, and proteins), respiratory system
Abstract

International audience; The mucociliary function of the bronchial epithelium ensures the continuous clearance of the respiratory system, which relies on two main elements: mucus and cilia beating coordination.We perform here a rheological characterization of mucus samples extracted from ALI (Air-liquid interface) cultures of bronchial epithelium. Our approach combines macro- and micro-rheology techniques with the aim of quantifying the mucus viscoelastic properties at different length scales (from the size of bronchial cilia up to the scale on which mucus is transported). This specific methodology allows us to compare samples corresponding to different patient pathologies.In addition, we will describe our method to quantitatively characterize the coordination between cilia and how density and spatial distribution influences this coordination and consequently the mucus motion, required for the mucoliary clearance.

Published
2017

32. Macro and Micro rheological characterization of Human bronchial mucus

Author

JORY, Myriam, Blanc, Christophe, Fort, Aurélie, Vachier, Isabelle, Bourdin, Arnaud, CASANELLAS VILAGELIU, Laura, MASSIERA, Gladys, Laboratoire Charles Coulomb (L2C), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Matière Molle, Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Service d'allergologie et de pneumologie [Hôpital Arnaud de Villeneuve], Centre Hospitalier Universitaire de Montpellier (CHU Montpellier ), The European Society of Rheology (ESR), and Projet de thèse: 'Approche biophysique de la fonction mucociliaire: rhéologie du mucus et coordination ciliaireCollaboration avec le CHU de Montpellier

Subjects
Mucus, Human bronchial epithelium, fluids and secretions, Optical Tweezers, [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], Micro rheology, Adhesion, respiratory system, Macro Rheology, respiratory tract diseases
Abstract

International audience; The mucociliary function of the bronchial epithelium ensures the continuous clearance of the respiratory system. This function relies on two main elements: mucus and cilia beating. We perform here a rheological characterization of mucus samples extracted from ALI (Air-liquid interface) cultures of bronchial epithelium. Our approach combines macro- and micro-rheology techniques with the aim of quantifying the mucus viscoelastic properties at different length scales (from the size of bronchial cilia up to the scale on which mucus is transported). In addition, this methodology allows us to compare the rheological behavior of mucus samples corresponding to different pathologies

Published
2017

33. Rhéologie du mucus à partir d'un modèle d'épithélium bronchique Humain

Author

JORY, Myriam, Blanc, Christophe, Fort, Aurélie, DONNARUMMA, Dario, CASANELLAS VILAGELIU, Laura, Vachier, Isabelle, Bourdin, Arnaud, MASSIERA, Gladys, Laboratoire Charles Coulomb (L2C), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Matière Molle, Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Service d'allergologie et de pneumologie [Hôpital Arnaud de Villeneuve], Centre Hospitalier Universitaire de Montpellier (CHU Montpellier ), Vaincre la Mucoviscidose, and Projet de thèse: 'Approche biophysique de la fonction mucociliaire: rhéologie du mucus et coordination ciliaireCollaboration avec le CHU de Montpellier

Subjects
Mucus, Human bronchial epithelium, Optical Tweezers, [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], Micro rheology, Adhesion, COPD, Macro Rheology
Abstract

National audience; La fonction mucociliaire de l'épithélium bronchique assure le nettoyage continu des voies respiratoires afin d’éviter toutes infections et inflammations par les éléments présents dans l’air que nous respirons. L'efficacité de la clairance mucociliaire repose sur deux éléments principaux : les propriétés rhéologiques du mucus et la coordination du battement ciliaire. Dans cette communication, nous décrirons spécifiquement nos résultats sur la rhéologie du mucus. Notre approche biophysique vise à comprendre les paramètres biochimiques et physiques affectant les propriétés viscoélastiques du mucus et à évaluer l 'utilisation des propriétés viscoélastiques du mucus comme marqueur du diagnostic de maladie respiratoire (BPCO, maladie pulmonaire obstructive chronique ...).Des études sur la rhéologie du mucus ont déjà été réalisées, mais essentiellement sur le crachat ou le mucus reconstitué. Nous avons effectué des expériences rhéologiques sur le mucus extrait des cultures ALI (Air Liquid Interface) de l'épithélium bronchique reconstitué à partir d'une biopsie bronchique. Notre approche consiste à combiner une expérience de macro-rhéologie standard et une micro-rhéologie réalisée avec des pinces optiques afin de quantifier les propriétés viscoélastiques du mucus et de comprendre son comportement d'écoulement à différentes échelles, de l'échelle des cils jusqu'à l'échelle de longueur sur laquelle le mucus est transporté.Nos résultats montrent le comportement d'amincissement par cisaillement et la nature élastique du mucus avec un petit module élastique G 'compris entre 1 et 2 Pa. On observe également la rupture du gel de mucus à très faible taux de cisaillement et même pour la déformation oscillatoire réalisée À des fréquences inférieures à la fréquence de battement des cils (~ 15 Hz), probablement en raison de la structure complexe et hétérogène du mucus. Néanmoins, la structure du mucus et les propriétés viscoélastiques sont récupérées en 5 minutes. La macro et la micro-rhéologie indiquent deux comportements différents : on observe un plateau élastique à la macro-échelle et un comportement visqueux à la micro-échelle, avec une viscoélasticité très faible de l'ordre de 10-3 à 10-2 Pa et proportionnelle À la fréquence de ω0,9 dans la même plage de fréquence. En appliquant des forces locales grâce à des pinces optiques, comme le montre la figure 1, nous montrons que l'adhérence du mucus est impliquée et doit être prise en compte pour comprendre les différences entre les propriétés mécaniques mesurées à ces différentes échelles de longueur et plus généralement pour caractériser les propriétés du mucus.Cette approche biophysique et méthodologie spécifique nous permet de comparer des échantillons correspondant à différentes pathologies du patient et pourrait être appliquée avec succès à l'hypersécrétion de mucus de patients atteints de fibrose kystique. Ce travail s'inscrit dans le cadre d'un projet de plus grande envergure impliquant une étude de physique statistique sur la coordination des cils et son couplage avec la viscoélasticité du mucus.

Published
2017

35. Additional file 3: Table S2. of DNA methylation at modifier genes of lung disease severity is altered in cystic fibrosis

Author

Magalhães, Milena, Rivals, Isabelle, Claustres, Mireille, Varilh, Jessica, Thomasset, Mélodie, Bergougnoux, Anne, Mely, Laurent, Leroy, Sylvie, Corvol, Harriet, Guillot, Loïc, Murris, Marlène, Beyne, Emmanuelle, Caimmi, Davide, Vachier, Isabelle, Chiron, Raphaël, and Sario, Albertina De

Abstract

Distribution of HMOX1 and GSTM3 genotypes in CF patients and controls (DOCX 39 kb)

Published
2017
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36. Additional file 3: Table S2. of DNA methylation at modifier genes of lung disease severity is altered in cystic fibrosis

Author

Magalhães, Milena, Rivals, Isabelle, Claustres, Mireille, Varilh, Jessica, Thomasset, Mélodie, Bergougnoux, Anne, Mely, Laurent, Leroy, Sylvie, Corvol, Harriet, Guillot, Loïc, Murris, Marlène, Beyne, Emmanuelle, Caimmi, Davide, Vachier, Isabelle, Chiron, Raphaël, and Sario, Albertina De

Abstract

Distribution of HMOX1 and GSTM3 genotypes in CF patients and controls (DOCX 39 kb)

Published
2017
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37. Additional file 2: Figure S2. of DNA methylation at modifier genes of lung disease severity is altered in cystic fibrosis

Author

Magalhães, Milena, Rivals, Isabelle, Claustres, Mireille, Varilh, Jessica, Thomasset, Mélodie, Bergougnoux, Anne, Mely, Laurent, Leroy, Sylvie, Corvol, Harriet, Guillot, Loïc, Murris, Marlène, Beyne, Emmanuelle, Caimmi, Davide, Vachier, Isabelle, Chiron, Raphaël, and Sario, Albertina De

Subjects
body regions, nervous system, fungi
Abstract

DNA methylation distribution at 14 analyzed genes in CF and control samples. (PDF 212 kb)

Published
2017
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40. Rising total costs and mortality rates associated with admissions due to COPD exacerbations

Author

Molinari , Nicolas, Chanez , Pascal, Roche , Nicolas, Ahmed , Engi, Vachier , Arnaud, Bourdin , Arnaud, Vachier , Isabelle, Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Pneumologie et Soins Intensifs Respiratoires, Université Paris Descartes - Paris 5 (UPD5)-Hôpitaux Universitaires Paris Centre, Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Département pneumologie et addictologie [Montpellier], Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Hôpital Arnaud de Villeneuve, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] ( PhyMedExp ), Centre National de la Recherche Scientifique ( CNRS ) -Université de Montpellier ( UM ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Centre Hospitalier Régional Universitaire [Montpellier] ( CHRU Montpellier ), Centre recherche en CardioVasculaire et Nutrition ( C2VN ), Institut National de la Recherche Agronomique ( INRA ) -Aix Marseille Université ( AMU ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Université Paris Descartes - Paris 5 ( UPD5 ) -Hôpitaux Universitaires Paris Centre, CHU Saint-Eloi-Centre Hospitalier Régional Universitaire [Montpellier] ( CHRU Montpellier ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université de Montpellier ( UM ), Centre Hospitalier Régional Universitaire [Montpellier] ( CHRU Montpellier ) -Hôpital Arnaud de Villeneuve, MORNET, Dominique, and Centre recherche en CardioVasculaire et Nutrition (C2VN)

Subjects
Male, Time Factors, Databases, Factual, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Severity of Illness Index, Pulmonary Disease, Chronic Obstructive, 0302 clinical medicine, Patient Admission, Risk Factors, 030212 general & internal medicine, Hospital Mortality, Longitudinal Studies, Registries, Hospital Costs, health care economics and organizations, ComputingMilieux_MISCELLANEOUS, Aged, 80 and over, COPD, Mortality rate, Incidence (epidemiology), Incidence, Process Assessment, Health Care, Middle Aged, 3. Good health, [SDV] Life Sciences [q-bio], Treatment Outcome, Disease Progression, Female, France, Pulmonary and Respiratory Medicine, medicine.medical_specialty, Critical Care, education, macromolecular substances, Bronchoscopies, 03 medical and health sciences, COPD exacerbation, Severity of illness, medicine, Humans, Intensive care medicine, Admissions, Aged, Mechanical ventilation, Hospitalizations, [ SDV ] Life Sciences [q-bio], business.industry, Research, COPD management, Length of Stay, medicine.disease, Respiration, Artificial, respiratory tract diseases, Pneumonia, 030228 respiratory system, Copd exacerbation, Deaths, Emergency medicine, business
Abstract

Background To examine trends in mortality, costs and in-hospital management and outcomes of severe COPD exacerbations admitted in France. Methods Patients hospitalized from 2007 to 2012 with COPD exacerbation as the primary diagnosis were identified from the exhaustive French medico-administrative hospitalizations database records. Four groups of severe COPD exacerbations were defined: hospitalisation in a general ward (GW) without acute respiratory failure (ARF), GW with ARF, ICU without invasive mechanical ventilation (MV), and ICU with MV. Results A 15.48 % increase in admissions from 113 276 in 2007 to 133 497 in 2012 was recorded. Age (+9.9 months), gender (−2.5 % of male) and length of stay (−0.29 day) slightly changed while the number of ICU admissions increased markedly (+41.78 %). In-hospital mortality rates increased (+8.06 %, p

Published
2016

41. Additional file 1: Table SA1. of Rising total costs and mortality rates associated with admissions due to COPD exacerbations

Author

Molinari, Nicolas, Chanez, Pascal, Roche, Nicolas, Engi Ahmed, Vachier, Isabelle, and Bourdin, Arnaud

Subjects
respiratory tract diseases
Abstract

COPD hospitalizations and definitions of severity categories (sorting rules according to ICD-10) [17]. Table SA2. Leading cause associated with COPD admissions. Table SA3. Trends in admissions-related costs among different categories of severity. Table SA4. Cause of COPD admissions. Only five leading causes are mentioned. Table SA5. Top seven medical acts performed during hospitalizations for COPD exacerbations. 2007 and 2012 data and changes are presented. (DOCX 27Â kb)

Published
2016
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42. Computed tomography quantification of airway remodelling in normal ageing subjects: a cross-sectional study: TABLE 1

Author

Bommart, Sebastien, Marin, Grégory, Bourdin, Arnaud, Revel, Marie Pierre, Klein, François, Hayot, Maurice, Vachier, Isabelle, Chanez, Pascal, Picot, Marie Christine, Mercier, Jacques, Molinari, Nicolas, Vernhet-Kovacsik, Hélène, Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] ( PhyMedExp ), Centre National de la Recherche Scientifique ( CNRS ) -Université de Montpellier ( UM ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Centre Hospitalier Régional Universitaire [Montpellier] ( CHRU Montpellier ), Université Paris Descartes - Paris 5 ( UPD5 ), Adhésion et Inflammation ( LAI ), Assistance Publique - Hôpitaux de Marseille ( APHM ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Aix Marseille Université ( AMU ) -Centre National de la Recherche Scientifique ( CNRS ), Service de Rhumatologie [CHU de Montpellier], CHU Montpellier, Laboratoire de Biostatistique, Epidémiologie et Recherche Clinique - IURC, Université Montpellier 1 ( UM1 ) -Université de Montpellier ( UM ), Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Université Paris Descartes - Paris 5 (UPD5), Adhésion et Inflammation (LAI), Assistance Publique - Hôpitaux de Marseille (APHM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Aide à la Décision pour une Médecine Personnalisé - Laboratoire de Biostatistique, Epidémiologie et Recherche Clinique - EA 2415 (AIDMP), Université Montpellier 1 (UM1)-Université de Montpellier (UM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Université de Montpellier (UM)-Université Montpellier 1 (UM1), and Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[ SDV ] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2015

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