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Free-space satellite communication has significantly lower photon loss than terrestrial communication via optical fibers. Satellite-based quantum key distribution (QKD) leverages this advantage and provides a promising direction in achieving long-distance inter-continental QKD. Satellite channels, however, can be highly dynamic due to various environmental factors and time-of-the-day effects, leading to heterogeneous noises over time. In this paper, we compare two key distillation techniques for satellite-based QKD. One is the traditional {\em non-blockwise} strategy that treats all the signals as a whole; the other is a {\em blockwise} strategy that divides the signals into individual blocks that have similar noise characteristics and processes them independently. Through extensive simulation in a wide range of settings, we show trends in optimal parameter choices and when one strategy provides better key generation rates than the other. Our results show that the blockwise strategy can lead to up to $5\%$ key rate improvement (leading to on average $1.9\times10^{7}$ more key bits per day) when considering two types of blocks, i.e., for nighttime and daytime, respectively. The blockwise strategy only requires changes in the classical post-processing stage of QKD and can be easily deployed in existing satellite systems.

La sarcoidosis es una enfermedad inflamatoria multisistémica granulomatosa, no necrotizante de etiología desconocida, su diagnóstico es clínico-patológico. Su incidencia se estima entre 2.3 y 11 por 100.000 habitantes/año y prevalencia varía de 2.17 a 160 por 100.000 personas, comienza en adultos menores de 50 años, pero se ha visto que alrededor del 70% de los casos ocurren entre los 25 y los 40 años en el momento de la presentación, con un segundo pico de incidencia en mujeres mayores de 50 años. Se presenta paciente masculino de 41 años, afroamericano, procedente de la región Nor-Oriental de Guatemala y residente en la ciudad de Guatemala, sin comorbilidades, con dermatosis caracterizada por: múltiples neoformaciones papuliformes, firmes de 3 a 4 mm de diámetro, de color negruzco, asociadas a prurito constante sin predominio de horario sobre tatuaje en miembro superior izquierdo, de dos años de evolución, sin fiebre, diaforesis nocturna, ni pérdida de peso, no recibió atención medica previamente. Exámenes de laboratorio sin alteraciones, to-mografía axial computarizada de pulmones sin afectación del parénquima pulmonar, análisis microbiológicos para tuberculosis fueron negativos, serología para: VIH, hepatitis A, B y C fueron negativas, VDRL no reactivo. Se le realiza biopsia incisional en sacabocados de 4 mm de piel del área del tatuaje, la histopatología reporto piel con inflamación crónica granulomatosa con for-mación de granulomas pequeños uniformes, con presencia de células gigantes de tipo cuerpo ex-traño, dentro de las cuales se observan cuerpos asteroides pequeños, tinción de Ácido periódico de Schiff (PAS) y Kinyoun fueron negativas para microrganismos, se llegó al diagnóstico de sarcoi-dosis extrapulmonar en piel. El diagnóstico de sarcoidosis se requiere un cuadro clínico sugestivo, la comprobación histopatológica de granulomas no caseificantes en los órganos afectos y la exclu-sión de otras enfermedades capaces de producir hallazgos histopatológicos, o clínicos similares. Palabras claves: Granulomas, Manifestaciones cutáneas, Sarcoidosis.

El síndrome de Brooke-Spiegler (SBS) es una enfermedad autosómica dominante infrecuente, los pacientes con el SBS presentan mutaciones en el gen supresor tumoral CYLD, más frecuente en mujeres que hombres, entre la segunda y tercera década de la vida con una relación hombre:mujer de 1:6-9.6 y su incidencia exacta se desconoce. Clínicamente se presentan de manera simultánea cilindroma, tricoepiteliomas y espiroadenomas. Se presenta el caso de una paciente femenina de 64 años, quien consultó por aparición de masas en cabeza, tronco y las cuatro extremidades de 10 años de evolución, posteriormente aparecieron lesiones en cuero cabelludo, donde el crecimiento tuvo una evolución más lenta y que llegaron a ulcerarse; busca atención medica hasta que las lesiones iniciaron a ulcerarse, por lo cual fue referida al Hospital General San Juan de Dios de la Ciudad de Guatemala, al servicio de dermatología, donde se le realizó hematología completa, química sanguínea, tomografía computarizada de cráneo y biopsia incisional en sacabocados de 4 mm de piel de frente, mentón, región preauricular derecha y pierna derecha, el estudio histopatológico de las lesiones cutáneas en las regiones preauricular derecha y de frente reporto cilindroma, piel de mentón tricoepitelioma y piel de pierna derecha espiroadenoma. Con los hallazgos clínicos, estudios de imagen y hallazgos histopatológico se llega al diagnóstico de síndrome de Brooke-Spiegler. El tratamiento fue la prescripción cuidados de piel y derivación a cirugía plástica para tratamiento quirúrgico reconstructivo y seguimiento por la consulta externa de dermatología y cirugía plástica. La presencia de cilindromas, tricoepiteliomas y espiroadenomas hacen pensar en un síndrome de Brooke-Spiegler, que tienden a tener presentaciones muy sugestivas, como el tumor en turbante y desfiguración de la región de la cabeza. El abordaje es multidisciplinario, sin un tratamiento definitivo y seguimiento para evaluar las posibles conversiones a malignidad de las lesiones ulceradas. Palabras claves: Cilindroma, CYLD, Espiroadenoma, Histopatología.

Leslie O’Dell,1 Damon S Dierker,2 Douglas K Devries,3 Jaclyn Garlich,4 Walter O Whitley,5 Mark Holdbrook,6 Stephanie N Baba,6 Elizabeth Yeu5 1Medical Optometry America, Shrewsbury, PA, USA; 2Eye Surgeons of Indiana, Indianapolis, IN, USA; 3Eye Care Associates of Nevada, Sparks, NV, USA; 4Envision Optometry, Boston, MA, USA; 5Virginia Eye Consultants, Norfolk, VA, USA; 6Tarsus Pharmaceuticals, Inc, Irvine, CA, USACorrespondence: Elizabeth Yeu, Virginia Eye Consultants, 241 Corporate Boulevard, Suite 210, Norfolk, VA, 23502, USA, Tel +1 757 793 4942, Fax +1 757 319 2493, Email eyeulin@gmail.comPurpose: To evaluate the impact of Demodex blepharitis on patients’ daily activities and quality of life.Patients and Methods: In this multicenter, observational, prospective, IRB-approved study, 311 Demodex blepharitis patients aged ≥ 18 years were included. Demodex blepharitis was diagnosed based on the presence of ≥ 1.0 mite per lash (upper and lower eyelids combined), > 10 collarettes on the upper lashes, and at least mild lid margin erythema of the upper eyelid in at least one eye. All patients were asked to complete a questionnaire about their symptoms, daily activities, quality of life, and management approaches, and descriptive statistics were used to analyze the responses.Results: More than half the patients had been experiencing symptoms of blepharitis for ≥ 4 years. The three most frequent and bothersome symptoms experienced by patients were dry eyes, itchiness, and irritation. Nearly half the patients (47%) responded that they were conscious of their eyes all day, and 23% said that they were constantly worrying about their eyes. Other activities that were negatively affected included difficulty driving at night (47%), additional time needed for daily hygiene routine (30%), and difficulty in wearing eye make-up (in 34% of females). While all subjects had objective signs of Demodex blepharitis confirmed by an eye care provider, 58% said they had never previously been diagnosed with blepharitis. The most commonly used management approaches for Demodex blepharitis were artificial tears (47%), warm compresses (32%), and lid wipes (14%).Conclusion: Demodex blepharitis has a significant negative impact on daily activities and the mental and physical well-being of afflicted patients.Keywords: Demodex blepharitis, dry eye disease, collarettes, cylindrical dandruff, Demodex mites, lid margin disease

Entropic uncertainty relations are powerful tools, especially in quantum cryptography. They typically bound the amount of uncertainty a third-party adversary may hold on a measurement outcome as a result of the measurement overlap. However, when the two measurement bases are biased towards one another, standard entropic uncertainty relations do not always provide optimal lower bounds on the entropy. Here, we derive a new entropic uncertainty relation, for certain quantum states and for instances where the two measurement bases are no longer mutually unbiased. We evaluate our bound on two different quantum cryptographic protocols, including BB84 with faulty/biased measurement devices, and show that our new bound can produce higher key-rates under several scenarios when compared with prior work using standard entropic uncertainty relations.

High-dimensional (HD) states are known to have several interesting properties when applied to quantum cryptography. For quantum key distribution (QKD), these states have the potential to improve noise tolerance and efficiency. However, creating, and measuring, HD states is technologically challenging, thus making it important to study HD-QKD protocols where Alice and Bob are restricted in their quantum capabilities. In this paper, we revisit a particular HD-QKD protocol, introduced in (PRA 97 (4):042348, 2018), which does not require Alice and Bob to be capable of sending and measuring in full mutually unbiased bases. In a way, the protocol is a HD version of the three state BB84: one full basis is used for key distillation, but only a single state is used, from an alternative basis, for testing the fidelity of the channel. The previous proof of security for this protocol has relied on numerical methods, making it difficult to evaluate for high dimensions. In this work, we provide a new proof of security, and give an explicit key-rate equation for depolarization channels, allowing us to evaluate the key-rate for arbitrarily high dimensional states. Furthermore, our new proof produces better results than prior work for dimensions greater than eight, and shows that HD-states can benefit restricted protocols of this nature.

Introduction Clinical utilization of pharmacogenomics (PGx) testing is highly institutionally dependent, and little information is known about provider practices of PGx testing in the Military Health System (MHS). In this study, we aimed to characterize Clinical Pharmacogenetics Implementation Consortium (CPIC) actionable prescription (Rx) patterns and their temporal relationship with PGx testing in the MHS. Methods Using data from the Military Health System Management Analysis and Reporting Tool (M2) database, this retrospective cohort study included all patients receiving at least one PGx test and at least one CPIC actionable Rx from January 2015 to August 2020 (845 patients, 1,471 PGx, 7,725 index CPIC actionable Rxs). Rx patterns and temporal relationships with PGx testing were characterized via descriptive statistics. Binomial regression was used to determine which patient and provider characteristics were associated with a patient receiving a PGx test within 30 days of an index Rx. Results Patients had a median of 9 index CPIC actionable Rx’s (range 1–26). Pain medications were most commonly prescribed (N = 794, 94% patients with at least 1 Rx). However, pain medication had the lowest Rx–PGx match rate (40%) compared to an average of 62% Rx–PGx match rate for all CPIC drugs. Antidepressants were also commonly prescribed (N = 668, 79.1% patients with at least 1 Rx), and antidepressants had the highest Rx–PGx match rate of 86.7%. A minority of providers (20%, N = 249) ordered the majority of PGx tests (86.1%, N = 1,266) and only 8.3% of PGx tests (N = 398) matched to a CPIC actionable drug within 30 days of the test (defined by Rxs ordered within 30 days before or after the PGx test). However, approximately 39.8% of patients (N = 317) had at least one drug match to a PGx test within 30 days. The largest predictor of whether a patient received a PGx test within 30 days of any index Rx was whether or not a specific psychiatry provider ordered the PGx test (odds ratio; OR 3.7, 95% CI 2.13–6.54, P Conclusions PGx testing was generally limited to high Rx-drug users and was found to be an under-utilized resource. PGx testing did not typically follow CPIC guidelines. Implementing PGx testing protocols, simplifying PGx test-ordering by incorporating at minimum CYP2D6, CYP2C19, and CYP2C9 into PGx-testing panels, and unifying providers’ PGx knowledgebase in the MHS are feasible and would improve the clinical utilization of PGx tests in the MHS.

Background: The negative impacts of the construction industry are compelling arguments for embracing technology that contributes to carbon footprint reduction and resources conservation. Toward the achievement of objective 9 of the Sustainable Development Goals, the development of new building’s materials like straw bale has advanced in the construction industry. As demonstrated in the literature, straw bale is an eco-friendly material that presents many advantages, like its contribution towards a circular economy. However, it has low compressive strength and displays high displacement under compression load. So far, no attempt has been made in order to enhance the strength of straw bales. Objective: This study aimed to develop alternative material to straw bale using chopped straw stems mixed with a binder (gum Arabic) and determine its stress-strain characteristic. Methods: The manufacturing process of the new material involved the use of chopped straw and gum Arabic to form straw blocks. Results: Results obtained show that the compressive strength of straw block (1.25MPa) is greater than the strength of straw bale (0.02MPa). Also, the average displacement recorded during compression load on straw blocks (29mm) was 2.8 times smaller than the displacement in straw bale (80mm). In terms of shape and size, straw blocks match with conventional materials like cement or compressed block. This will facilitate their use in construction compared to straw bales that require skilled laborers for pre-compression and plastering. Conclusion: The use of gum arabic helps in holding straw stems together and forms a compact material with improved strength compared to straw bale. Performance improvement of the characteristics of load-bearing straw bale walls can be addressed by using straw blocks.

Introduction Existing data on cardiovascular disease among transgender people are inconsistent and are derived from nonrepresentative samples or population-based data sets that do not include transgender-specific risk factors such as gender-affirming hormone use and gender minority stressors. A nationally representative sample of cisgender and transgender adults aged ≥40 years was used to assess the prevalence and correlates of smoking, select cardiovascular disease conditions, and venous thromboembolism. Methods Participants were recruited from 2016 to 2018, with analysis conducted in December 2020 with 114 transgender and 964 cisgender individuals. Sample weights and multiple imputations were used for all estimates except for descriptive statistics. Logistic regression models estimated the ORs and 95% CIs expressing the relationship between each outcome variable and a set of independent variables. Each model controlled for race and age. Results No meaningful differences between cisgender and transgender participants were found in smoking or cardiovascular disease conditions. However, there was an increased odds of venous thromboembolism among transgender women compared with those among cisgender women. Transgender people had greater odds of discrimination, psychological distress, and adverse childhood experiences. These stressors were associated with increased odds of a cardiovascular condition, and everyday discrimination and adverse childhood experiences were associated with increased odds of smoking. Discrimination and psychological distress were associated with venous thromboembolism. Conclusions Transgender people face disparities in cardiovascular disease risk. This study provides support for the gender minority stress model as a framework for understanding cardiovascular disease disparities. Future research with larger samples and adjudicated outcomes is needed to advance the field.

Perovskite solar cells (PSC) are under focus in the field of renewable energies due to the exceptional photoconversion efficiencies achieved in a short period of time. Even though the laboratory reports are very promising, it is necessary to perform a scale-up feasibility analysis to determine if PSCs can become a widely used technology. In this report we evaluated the energy performance with energy indicators from Net Energy Analysis (NEA). We performed a cradle-to-gate life cycle assessment (LCA) of a n-i-p PSCs fabricated in the Buenos Aires city area to focus on the energy supply from the life cycle inventory. The energy payback time (EPBT) and the energy return of investment (EROI) were analyzed in order to examine the energy generation and the lifetime requirements of the perovskite photovoltaic modules to provide a resourceful commercialization pathway. The EPBT was analyzed under different solar irradiation values for varied locations throughout the vast Argentinian territory, that ranges between 0.97 and 1.74 years for 15% efficiency modules. A further evaluation of the addition of an extra encapsulation step for stability purposes was also studied. We also analyzed the inverted configuration of MAPbI3 solar cells in order to compare both production process and their effect on the energy performance. We were able to state that the different processing energy provides better outcomes for the p-i-n configuration and must be prioritized for commercialization purposes.

Background and Aim: Momordica charantia is mainly characterized by its antimicrobial and antioxidant properties. The current study aimed to evaluate the healing activity of gel and cream formulations based on M. charantia on induced wounds in mice. Materials and Methods: Acetonic extract of M. charantia was prepared and incorporated into gel and cream formulations. Mus musculus Balb/c (n=30) with induced injury were distributed into five groups: Group I (control – day 7), Group II (control – day 14), Group III (1% gel – day 7), and Group IV (1% gel – day 14) to which 1% M. charantia gel was dermally applied daily for 7 and 14 days, respectively, Group V (1% cream – day 7) and Group VI (1% cream – day 14) to which of M. charantia 1% cream were dermally applied daily for 7 and 14 days, respectively. Time of wound closure was determined during the experimentation; rats were euthanized with sodium pentobarbital 60 mg/kg/pc v.ip. for obtaining skin samples for histopathological analysis. Results: Groups IV and VI showed a higher percentage of wound closure on day 14, and in histopathological analysis, effect was greater in Group VI with the presence of fibroblasts and abundant collagen and elastic fibers. Conclusion: M. charantia gel and cream showed wound healing activity on induced wounded mice; the most effective treatment was M. charantia 1% cream formulation.

Gastrointestinal diseases, such as peptic ulcers, are caused by a damage in the gastric mucosa provoked by several factors. This stomach injury is regulated by many inflammatory mediators and is commonly treated with proton-pump inhibitors, histamine H2 receptor blockers and antacids. However, various medicinal plants have demonstrated positive effects on gastric ulcer treatment, including plants of the Ceiba genus. The aim of this study was to evaluate the antiulcer and anti-inflammatory activities of the stem bark ethanolic extract of Ceiba speciosa (A. St.-Hil.) Ravenna. We performed a preliminary quantification of phenolic compounds by high-performance liquid chromatography-diode array detection (HPLC-DAD), followed by the prospection of other chemical groups through nuclear magnetic resonance (NMR) spectroscopy. A set of in vitro assays was used to evaluate the extract potential regarding its antioxidant activity (DPPH: 19.83 ± 0.34 µg/mL; TPC: 307.20 ± 6.20 mg GAE/g of extract), effects on cell viability and on the release of TNF-α in whole human blood. Additionally, in vivo assays were performed to evaluate the leukocyte accumulation and total protein quantification in carrageenan-induced air pouch, as well as the antiulcerogenic effect of the extract on an ethanol-induced ulcer in rats. The extract contains flavonoids and phenolic compounds, as well as sugars and quinic acid derivatives exhibiting potent antioxidant activity and low toxicity. The extract reduced the release of TNF-α in human blood and inhibited the activity of p38α (1.66 µg/mL), JAK3 (5.25 µg/mL), and JNK3 (8.34 µg/mL). Moreover, it reduced the leukocyte recruitment on the pouch exudate and the formation of edema, reverting the effects caused by carrageenan. The extract presented a significant prevention of ulcer formation and a higher reduction than the reference drug, Omeprazole. Therefore, C. speciosa extract has demonstrated relevant therapeutic potential for the treatment of gastric diseases, deserving the continuation of further studies to unveil the mechanisms of action of plant bioactive ingredients.

Semi-quantum cryptography involves at least one user who is semi-quantum or "classical" in nature. Such a user can only interact with the quantum channel in a very restricted way. Many semi-quantum key distribution protocols have been developed, some with rigorous proofs of security. Here we show for the first time, to our knowledge, that quantum random number generation is possible in the semi-quantum setting. We also develop a rigorous proof of security, deriving a bound on the random bit generation rate of the protocol as a function of noise in the channel. Our protocol and proof may be broadly applicable to other quantum and semi-quantum cryptographic scenarios where users are limited in their capabilities.

Gastrointestinal diseases, such as peptic ulcers, are caused by a damage in the gastric mucosa provoked by several factors. This stomach injury is regulated by many inflammatory mediators and is commonly treated with proton-pump inhibitors, histamine H2 receptor blockers and antacids. However, various medicinal plants have demonstrated positive effects on gastric ulcer treatment, including plants of the

Africa has received considerable amounts of external aid over the last two decades without significant improvements in socio-economic conditions on the continent. This study, therefore, examines the effects of foreign aid on growth in Africa, and how institutional quality can moderate these effects. The study used the system generalized method of moments estimation technique and a panel of forty-two African countries over the period 2010-2018. Interestingly, the study established that even though foreign aid impacts negatively on growth in Africa, improving the quality of institutions on the continent can reverse this negative effect. In fact, the study computed a threshold value of institutional quality beyond which foreign aid would be a blessing to Africa. This implies that for foreign aid to contribute meaningfully to growth in Africa, the quality of institutions should improve beyond this threshold. Unfortunately, the average level of institutional quality in Africa is presently below this threshold. The study concluded that policymakers in Africa should take urgent steps to strengthen the quality of institutions on the continent as a means of exploiting the continent's huge foreign aid to drive growth and reduce the excruciating effects of poverty plaguing more than half of its population.

Ondine Walter,1,2 Julien Maquet,1,2 Helene Derumeaux,3 Guillaume Moulis,1,2 Margaux Lafaurie2,4 1Service de Médecine Interne, Centre Hospitalier Universitaire de Toulouse, Toulouse, France; 2Centre d’Investigation Clinique 1436, Centre Hospitalier Universitaire de Toulouse, Toulouse, France; 3Département d’Information Médicale, Centre Hospitalier Universitaire de Toulouse, Toulouse, France; 4Service de Pharmacologie Médicale et Clinique, Faculté de Médecine, Centre Hospitalier Universitaire de Toulouse, Toulouse, FranceCorrespondence: Ondine WalterService de Médecine Interne, pavillon URM, CHU Purpan, place du Dr Baylac, TSA 40031, 31059, Toulouse Cedex 9, FranceTel +33 561 779 677Fax +33 561 778 058Email walter.o@chu-toulouse.frKeywords: sickle cell anemia, vaso occlusive crisis, predictive value of test, epidemiology

Se determinó la respuesta inmunológica a proteínas recombinantes de Helicobacter pylori en pacientes dispépticos (adultos y niños), pacientes con cáncer gástrico y sus familiares asintomáticos adultos viviendo con ellos. Se utilizó la prueba recomLine® Helicobacter IgG e IgA, y con base en el reconocimiento de los factores de virulencia VacA y CagA se determinó si la cepa de H. pylori era de tipo I o II. El análisis de los datos fue descriptivo y analítico y se estimaron los intervalos de confianza de 95%, con un nivel de error de 0.05 y Odds ratio. El 58.7% (121/206) de los pacientes presentó la bacteria en tinción histológica de biopsia, positividad que disminuyó con la edad y daño histológico. La frecuencia de la respuesta a los anticuerpos IgG fue mayor que IgA, en ambos casos ésta fue menor en los niños. Las proteínas del H. pylori más reconocidas tanto por IgA como IgG fueron VacA y CagA, y la respuesta a las otras proteínas investigadas fue mayor al aumentar el daño histológico. La cepa tipo I fue la que predominó en la población en estudio con 66% (136/206). Se deben continuar con los estudios de prevalencia de la cepa tipo I del H. pylori y del reconocimiento de sus antígenos en la población guatemalteca a fin de determinar su utilidad en el diagnóstico y pronóstico de la infección.

Aims Accidental contact with the Lonomia obliqua caterpillar is a common event in southern Brazil. Envenomed victims present consumption coagulopathy, which can evolve to acute kidney injury (AKI). In the present study, we searched for AKI biomarkers and changes in molecular pathway signatures through urine proteomic analysis. Methodology Male Wistar rats were injected with L. obliqua venom (1.5 mg/kg, via s.c.) or 0.9 % NaCl and distributed into metabolic cages. After 24 h, urine was obtained, and the set of differentially regulated proteins was analyzed by MudPIT technology in an OrbiTRAP mass spectrometer. Results L. obliqua venom leads to an increase in urine output and water and electrolyte excretion and to an increase in the albumin to creatine ratio in urine. The proteomic analysis revealed an up-regulation of tubular injury biomarkers, such as neutrophil-gelatinase associated lipocalin (NGAL) and cystatin C, in urine from envenomed rats. Several components related to the heme scavenging system were up-regulated or exclusively identified in urine from envenomed animals. There was an increase in urinary heme levels and hemoglobin subunits, hemopexin, haptoglobin, and biliverdin reductase. Similarly, kinin- and angiotensin-generating/degrading peptidases, such as kallikreins, neprilysin, plasmin, dipeptidyl peptidase IV, cathepsin D, kininogen, and neutral, basic, glutamyl, and acidic aminopeptidases, were also up-regulated in urine. Conclusions L. obliqua envenomation induced tubular and glomerular injury, probably involving heme/hemoglobin toxicity and an imbalance in the kinin/angiotensin generating/degrading system.

The study examined the influence of rural-urban migration on waste management in the Enugu metropolis. It was motivated by the waste disposal problems and challenges in the coal city. The specific objectives sought were to: determine the impact of rural-urban migration, the influence of irregular collection and disposal of refuse, and the influence of indiscriminate dumping of refuse on waste management in the Enugu metropolis. A survey design was adopted for the study. The area of study was the Enugu metropolis which includes Enugu East, Enugu South and Enugu North local government areas. The study covered activities of Enugu State Waste Management Agency, household residents, as well as contractors on waste management in the Enugu metropolis. The study population, 722,664, was drawn from the three local governments areas mentioned. The instrument for data collection was a structured questionnaire. Data collected were presented in Likert 5-points scale, and analysed with a measure of central tendency (Mean). Results indicate that increase in rural-urban migration have a negative impact on waste generation and management in Enugu State. The study recommended among other things that the Enugu State government should try to stem the tide of rural-urban migration and also develop strategies to control indiscriminate dumping of refuse in the city while ensuring that refuse collection and disposal are timely and regularly done across the Enugu metropolis.

International audience

Following health agencies warning, the use of animal origin supplements should be avoided in biological products proposed as therapy in humans. Platelet lysate and several other growth factors sources are alternatives to replace fetal calf serum, the current gold standard in clinical-grade cell culture. However, the platelet supplement's content lacks data due to different production methods. The principle behind these products relays on the lysis of platelets that release several proteins, some of which are contained in heterogeneous granules and coordinate biological functions. This study aims to analyze the composition and reproducibility of a platelet lysate produced with a standardized method, by describing several batches' protein and particle content using proteomics and dynamic light scattering. Proteomics data revealed a diversified protein content, with some related to essential cellular processes such as proliferation, morphogenesis, differentiation, biosynthesis, adhesion, and metabolism. It also detected proteins responsible for activation and binding of transforming growth factor beta, hepatocyte growth factor, and insulin-like growth factor. Total protein, biochemical, and growth factors quantitative data showed consistent and reproducible values across batches. Novel data on two major particle populations is presented, with high dispersion level at 231 ± 96 d.nm and at 30 ± 8 d.nm, possibly being an important way of protein trafficking through the cellular microenvironment. This experimental and descriptive analysis aims to support the content definition and quality criteria of a cell supplement for clinical applications.

Refereed/Peer-reviewed The spread of antimicrobial resistance (AMR) is a rapidly growing threat to humankind on both regional and global scales. As countries worldwide prepare to embrace a One Health approach to AMR management, which is one that recognizes the interconnectivity between human, animal, and environmental health, increasing attention is being paid to identifying and monitoring key contributing factors and critical control points. Presently, AMR sensing technologies have significantly progressed phenotypic antimicrobial susceptibility testing (AST) and genotypic antimicrobial resistance gene (ARG) detection in human healthcare. For effective AMR management, an evolution of innovative sensing technologies is needed for tackling the unique challenges of interconnected AMR across various and different health domains. This review comprehensively discusses the modern state-of-play for innovative commercial and emerging AMR sensing technologies, including sequencing, microfluidic, and miniaturized point-of-need platforms. With a unique view toward the future of One Health, we also provide our perspectives and outlook on the constantly changing landscape of AMR sensing technologies beyond the human health domain.

Quantum technology, and especially quantum computing, is advancing rapidly. Our every-day secure communication infrastructure relies heavily on public key cryptography; unfortunately, many public key schemes are in fact insecure against quantum algorithms. Furthermore, adversaries who, today, capture information encrypted using classical key distribution systems, can wait and, as soon as quantum computers of sufficient power become available, they may decipher all previously captured information. While some new “post-quantum” public key systems are assumed to be secure against quantum computers, this is only an assumption and, even if the assumption remains valid, still results in systems that are less efficient for end-users than the more commonly used public key systems employed today. While the advent of quantum technology may seem detrimental to security, in fact, one may harness the power of quantum through quantum key distribution (QKD) for stronger security guarantees. Such QKD protocols allow two (or more) parties to establish a shared secret key, secure against an all-powerful adversary. Perhaps surprising is that QKD technology is already here both experimentally and commercially. This tutorial serves as an introduction to basic QKD along with QKD technology from a practical perspective. Quantum communication is a highly interdisciplinary field of research and one of the goals of this article is to introduce a larger set of researchers and practitioners to its study in the hopes of furthering its progress and eventual wide-scale adoption.

Communication has always played a vital role in microgrids to maintain reliable operations and achieve great benefits and will be even more critical with the increasing deployment of renewable energies, information technologies, and real-time automation and control systems. The existing classical cryptographic methods for securing microgrid communication, however, rely on mathematical assumptions, which are vulnerable to attacks from quantum computers. This article reviews the current status of developing quantum-secure microgrids, namely microgrids that are secure against attacks from quantum computers. We introduce some potential issues associated with applying existing quantum cryptography methods in the context of microgrids and provide future perspectives to make quantum security more practical in microgrids.

The COVID-19 pandemic caused by the new coronavirus (SARS-CoV-2) has become a global emergency issue for public health. This threat has led to an acceleration in related research and, consequently, an unprecedented volume of clinical and experimental data that include changes in gene expression resulting from infection. The SARS-CoV-2 infection database (SARSCOVIDB: https://sarscovidb.org/) was created to mitigate the difficulties related to this scenario. The SARSCOVIDB is an online platform that aims to integrate all differential gene expression data, at messenger RNA and protein levels, helping to speed up analysis and research on the molecular impact of COVID-19. The database can be searched from different experimental perspectives and presents all related information from published data, such as viral strains, hosts, methodological approaches (proteomics or transcriptomics), genes/proteins, and samples (clinical or experimental). All information was taken from 24 articles related to analyses of differential gene expression out of 5,554 COVID-19/SARS-CoV-2-related articles published so far. The database features 12,535 genes whose expression has been identified as altered due to SARS-CoV-2 infection. Thus, the SARSCOVIDB is a new resource to support the health workers and the scientific community in understanding the pathogenesis and molecular impact caused by SARS-CoV-2.

Zika virus (ZIKV) infection during pregnancy was associated with microcephaly in neonates, but clinical and experimental evidence indicate that ZIKV also causes neurological complications in adults. However, the changes in neuron-glial communication, which is essential for brain homeostasis, are still unknown. Here, we report that hippocampal slices from adult rats exposed acutely to ZIKV showed significant cellular alterations regarding to redox homeostasis, inflammatory process, neurotrophic functions and molecular signalling pathways associated with neurons and glial cells. Our findings support the hypothesis that ZIKV is highly neurotropic and its infection readily induces an inflammatory response, characterized by an increased expression and/or release of pro-inflammatory cytokines. We also observed changes in neural parameters, such as adenosine receptor A2a expression, as well as in the release of brain-derived neurotrophic factor and neuron-specific enolase, indicating plasticity synaptic impairment/neuronal damage. In addition, ZIKV induced a glial commitment, with alterations in specific and functional parameters such as aquaporin 4 expression, S100B secretion and glutathione synthesis. ZIKV also induced p21 senescence-associated gene expression, indicating that ZIKV may induce early senescence. Taken together, our results indicate that ZIKV-induced neuroinflammation, involving nuclear factor erythroid 2-related factor 2 (Nrf2) and nuclear factor κB (NFκB) pathways, affects important aspects of neuron-glia communication. Therefore, although ZIKV infection is transient, long-term consequences might be associated with neurological and/or neurodegenerative diseases.

Background: In recent decades, the enduring interest and continued development of straw bale as a walling material are based on its beneficial properties. Straw bale is a biomaterial that contributes greatly to carbon footprint reduction and offers excellent thermal insulation. It is proved that plastered straw bale assemblies have good mechanical properties and can be used for the construction of a single storey building. It is known that straw bale presents high displacement in the assemblies; thus, pre-compression is a major step that helps to push down straw bale so as to avoid future structural failure in the wall. There is no clue yet if this method is structurally beneficial than to stabilized single straw bales before assembling them into a structural panel. Objective: This paper presents the structural performance of straw block assemblies under compression loads. Method: Straw blocks and mortar were used to construct plastered and un-plastered wall panels, which were tested under uniformly distributed compression load till failure. Results: The results obtained show that plastered straw block assemblies can support at least 286 KN/m2, which is higher than the minimum slab load 18.25KN/m2, including imposed load for a residential house. In addition, the strength of plastered straw block assemblies plastered with cement-gum mortar, 0.3 N/ mm2 is greater than the strength of a single storey building (0.19N/mm2). Furthermore, results indicate that un-plastered and plastered straw block assemblies perform better than un-plastered and plastered straw bale assemblies. Plastered straw block assemblies support up to 52KN while plastered straw bale assemblies support only 41.1KN. Conclusion: Under compression load, straw block assemblies have a load carrying capacity greater than the minimum slab load. Therefore, Straw block can be used for the construction of a single storey building.

In the United States, ~1.4 million individuals identify as transgender. Many transgender adolescents experience gender dysphoria related to incongruence between their gender identity and sex assigned at birth. This dysphoria may worsen as puberty progresses. Puberty suppression by gonadotropin-releasing hormone agonists (GnRHa), such as leuprolide, can help alleviate gender dysphoria and provide additional time before irreversible changes in secondary sex characteristics may be initiated through feminizing or masculinizing hormone therapy congruent with the adolescent’s gender experience. However, the effects of GnRH agonists on brain function and mental health are not well understood. Here, we investigated the effects of leuprolide on reproductive function, social and affective behavior, cognition, and brain activity in a rodent model. Six-week-old male and female C57BL/6J mice were injected daily with saline or leuprolide (20 μg) for 6 weeks and tested in several behavioral assays. We found that leuprolide increases hyperlocomotion, changes social preference, and increases neuroendocrine stress responses in male mice, while the same treatment increases hyponeophagia and despair-like behavior in females. Neuronal hyperactivity was found in the dentate gyrus (DG) of leuprolide-treated females, but not males, consistent with the elevation in hyponeophagia and despair-like behavior in females. These data show for the first time that GnRH agonist treatment after puberty onset exerts sex-specific effects on social- and affective behavior, stress regulation, and neural activity. Investigating the behavioral and neurobiological effects of GnRH agonists in mice will be important to better guide the investigation of potential consequences of this treatment for youth experiencing gender dysphoria.

Objectives (1) To study the reliability of quantifying rear-chain stabilization capacity during deadbug bridging (DBB), (2) to provide reference values for competitive alpine skiers, and (3) to study associations with age, anthropometrics, maturation, skiing performance and back overuse complaints. Design Cross-sectional. Setting Biomechanical field experiment including questionnaires. Participants 12 healthy subjects (reliability experiment); 133 skiers of the U16 category and 38 of the elite category (main experiment). Main outcome measures DBB performance was quantified using 3D motion capture as the maximum amplitude of the relative vertical displacement of two pelvis markers (DBBdisplacement). Additionally, in U16 skiers, age, anthropometrics, maturation, skiing performance, and back overuse complaints were assessed. Results The reliability experiment revealed an ICC(3,1) and 95% CI of 0.81 [0.61, 0.93]. Within-subject SEM was 3.89 mm [3.16 mm, 5.12 mm]. Depending on sex and category, medians of DBBdisplacement in skiers ranged between 29 mm and 45 mm. DBBdisplacement differed between elite and U16 skiers (p Conclusion The proposed approach may be considered an adequate method to quantify athletes’ rear-chain stabilization capacity.

Pectinases and other carbohydrate-active enzymes are important for the food industry, mainly for juice processing. In addition, the use of peels to produce enzymes can aggregate value to these agro-industrial residues and at the end of the process enhance qualitatively and quantitatively the juice production. In this work, three different extracts produced by Penicillium oxalicum LS09 using agro-industrial residues were optimized and analyzed by mass spectrometry. It was observed an increased production of pectinases in the medium containing orange peel and optimized for production of pectin lyase and pectinesterase (PE). Interestingly, not only pectinases, but also different plant cell wall degrading enzymes (i.e. glucanases, xylanases, arabinases), with a higher ratio (42/73) was identified in the medium optimized for PE. The crude extracts produced by P. oxalicum also reveal the potential for application in the fruit juice industry, showing an increased yield and qualitative characteristics of extracted juices. The presence of other cell wall-degrading enzymes identified by proteomics, reinforce the combination for obtaining clarified and depectinized juice in a single step. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13197-020-04688-9) contains supplementary material, which is available to authorized users.

Male infertility or subfertility is frequently associated with disruption of the hypothalamic-pituitary-testis axis events, like secondary hypogonadism. However, little is known how this condition affects the proteomic composition of the epididymal fluid. In the present study, we evaluated the proteomic changes in the cauda epididymal fluid (CEF) in a swine model of secondary hypogonadism induced by anti-GnRH immunization using multidimensional protein identification technology. Seven hundred and eighteen proteins were identified in both GnRH-immunized and control groups. GnRH immunization doubled the number of proteins in the CEF, with 417 proteins being found exclusively in samples from GnRH-immunized boars. CEF from GnRH-immunized boars presented an increase in the number of proteins related to cellular and metabolic processes, with affinity to organic cyclic compounds, small molecules, and heterocyclic compounds, as well changed the enzymatic profile of the CEF. Also, a significant increase in the number of proteins associated to the ubiquitin-proteasome system was identified in CEF from GnRH-immunized animals. These results bring strong evidence of the impact of secondary hypogonadism on the epididymal environment, which is responsible for sperm maturation and storage prior ejaculation. Finally, the differently expressed proteins in the CEF are putative seminal biomarkers for testicular and epididymal disorders caused by secondary hypogonadism.

Alpine ski racing is known as a high-risk sport; however, little is known on the health problems occurring at youth level. The purpose of this study was to investigate the prevalence of health problems in youth competitive alpine skiers with respect to sex, age, and season differences, to describe their severity and location and to assess the influence of biological maturation. Over a 12-month period, 155 youth competitive alpine skiers around the growth spurt were prospectively monitored for health problems using the Oslo Sports Trauma Research Centre (OSTRC) questionnaire. These data were verified by supplemental interviews at the end. Biological maturation was estimated by a non-invasive, anthropometric-based assessment method. During competition season, 42.2% of the skiers suffered from health problems and 19.8% reported their problem being substantial. Females had significantly higher rates of acute and overuse injuries compared to males, while there was no sex difference in duration, severity, and time loss. Skiers of the category U15 showed higher prevalence of acute and overuse injuries than U14 skiers. During preparation season, health problem prevalence was substantially lower. The knee was found to be the most affected body part for both acute and overuse injuries. There was a direct association between the offset to the age at peak height velocity (APHV) and the occurrence and severity of acute injuries. In conclusion, youth competitive alpine skiers suffer from a large number of health problems. Since sex, age, and biological maturation are important, effective injury prevention should already start before reaching the APHV.

PurposeThis paper analyzes the impact of obesity on the probability of a motor vehicle fatality (highway death rate) and on its component probabilities: the probability of a fatality, given a crash (vulnerability rate) and the probability of a crash (crash rate).Design/methodology/approachUsing state-level data for 1995–2015, the paper estimates models explaining all three rates. Explanatory factors include obesity and a representative set of potential determinants.FindingsResults indicate that obesity has a statistically significant positive relationship with the highway death rate and the crash rate. Also having a statistically significant positive association with at least one of the three rates are the proportions of young and old drivers, alcohol consumption, the ratio of rural to urban vehicle miles and temperature. Factors with a statistically significant negative relationship with at least one of the rates include primary seat belt laws and precipitation. In 2016, a total of 928 traffic fatalities could have been avoided if obesity rates decreased by one percentage point.Practical implicationsSeat belts and crash dummies should be better designed to fit and represent those with higher BMIs, and education efforts to increase seat belt use should be supplemented with information about the adverse impact of obesity on highway safety.Originality/valueThis paper uses 21 years of state-level information, including socio-economic and regulation data, and contributes to the existing research on the relationship between obesity and highway safety.

Numerous studies investigated the association between dynamic knee valgus and injury risk in post-pubertal and elite athletes; however, normative reference scores for competitive alpine skiers and observations on the development process throughout and beyond athletes' growth spurt are lacking. Thus, the aim of this study was to describe the dynamic knee valgus of competitive alpine skiers during drop jump landings (DJ) and single-leg squats (SLS) with respect to sex, sportive level, and biological maturation. Thirty-seven elite and 104 youth competitive alpine skiers around the growth spurt (U15) were examined for their maximal medial knee displacement (MKD) during DJ and SLS by a marker-based 3D motion analysis evaluating dynamic knee valgus. Additionally, skiers' age, anthropometry and biological maturation were assessed. MKD of youth and elite alpine skiers during DJ was comparable and did not improve with increasing training age. Female U15 skiers (on average further matured) had significantly larger MKD values during DJ than male U15 skiers (less matured) (P

Patellar tendon (PT) complaints are frequent throughout the population, with increased occurrence in athletes and, particularly, in youth competitive alpine skiers. Timely detection and treatment might improve prospects of recovery. Diagnostic modalities in clinical use to date rely on pain symptoms, manual palpation, and potentially, magnetic resonance imaging (MRI); however, MRI-based imaging yields limited sensitivity. Quantitatively measuring the morphological and mechanical properties of PTs by means of B-mode ultrasound and shear wave elastography (SWE), instead, may allow improved diagnosis or even early detection. We performed B-mode scans and three-dimensional ultrasound shear wave velocity (SWV) mapping and MRI of the PT in 106 youth skiers. A prospective one-year survey on health problems combined with clinical assessments served to categorize symptomatic and asymptomatic youth skiers. Skiers suffering from distal or proximal tendon complaints showed lower SWV in the respective tendon region than asymptomatic skiers (p = 0.035 and p = 0.019, respectively). Youth skiers with distal tendon complaints additionally exhibited decreased SWV in the proximal region compared to asymptomatic counterparts (p = 0.020). Cross-validated analysis of retrospective prediction indicated sensitivity and specificity in detecting tendon complaints in the range of 0.606-0.621 and 0.536-0.650, respectively. MRI detected distal tendon complaints with a sensitivity of 0.410 (12/29) but failed to detect any proximal cases. This study agrees with the most recent literature in that SWE holds promise as a valuable adjunct modality for the diagnosis of PT complaints or even the detection of subclinical prestages. However, to evaluate its prospective predictive value, long-term studies are warranted., European Journal of Sport Science, 23 (6), ISSN:1746-1391, ISSN:1536-7290

The acidity of soils significantly reduces the productivity of legumes mainly because of the detrimental effects of hydrogen ions on the legume plants, leading to the establishment of an inefficient symbiosis and poor biological nitrogen fixation. We recently reported the analysis of the fully sequenced genome of Rhizobium favelukesii LPU83, an alfalfa-nodulating rhizobium with a remarkable ability to grow, nodulate and compete in acidic conditions. To gain more insight into the genetic mechanisms leading to acid tolerance in R. favelukesii LPU83, we constructed a transposon mutant library and screened for mutants displaying a more acid-sensitive phenotype than the parental strain. We identified mutant Tn833 carrying a single-transposon insertion within LPU83_2531, an uncharacterized short ORF located immediately upstream from ubiF homolog. This gene encodes a protein with an enzymatic activity involved in the biosynthesis of ubiquinone. As the transposon was inserted near the 3′ end of LPU83_2531 and these genes are cotranscribed as a part of the same operon, we hypothesized that the phenotype in Tn833 is most likely due to a polar effect on ubiF transcription. We found that a mutant in ubiF was impaired to grow at low pH and other abiotic stresses including 5 mM ascorbate and 0.500 mM Zn(2+). Although the ubiF mutant retained the ability to nodulate alfalfa and Phaseolus vulgaris, it was unable to compete with the R. favelukesii LPU83 wild-type strain for nodulation in Medicago sativa and P. vulgaris, suggesting that ubiF is important for competitiveness. Here, we report for the first time an ubiF homolog being essential for nodulation competitiveness and tolerance to specific stresses in rhizobia. GRAPHICAL ABSTRACT: [Image: see text]

To evaluate the impact ofIn this multicenter, observational, prospective, IRB-approved study, 311More than half the patients had been experiencing symptoms of blepharitis for ≥4 years. The three most frequent and bothersome symptoms experienced by patients were dry eyes, itchiness, and irritation. Nearly half the patients (47%) responded that they were conscious of their eyes all day, and 23% said that they were constantly worrying about their eyes. Other activities that were negatively affected included difficulty driving at night (47%), additional time needed for daily hygiene routine (30%), and difficulty in wearing eye make-up (in 34% of females). While all subjects had objective signs of