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Additional file 3: Table S1. Antibodies used for Western blotting validation. Table S2 Target proteins identified by CQP-based ABPP. Table S3 Potential hits identified by MS-CETSA.

Additional file 1: Chemical synthesis schemes, NMR and MS Spectra, and supplementary methods.

The Hefei Light Source-II (HLS-II) is a vacuum ultraviolet synchrotron light source. The Personnel Safety System (PSS) is the crucial part to protect staff and users from radiation damages. In order to share access control information and improve the reliability for HLS-II, the novel PSS is designed based on Siemens redundant PLC under EPICS environment which is composed by the safety interlock system, access control system and the radiation monitoring system. This paper will demonstrate the architecture and the specific design of this novel PSS and shows the operation performance after it has been implemented for 2 years., Proceedings of the 18th International Conference on Accelerator and Large Experimental Physics Control Systems, ICALEPCS2021, Shanghai, China

Additional file 2: Table S1. Short interfering RNA sequences used to knockdown the PKM2 gene in macrophages.

Additional file 2: Fig. S1. Chemistry structures of TAMRA-N3 (A) and Biotin-N3 (B) of CuAAC-based click chemistry reaction. Fig. S2 Heat map representation of the target proteins dataset identified by the CQP-based ABPP. The expression levels of all proteins are standardized to Z-score values. Fig. S3 Gene Ontology (GO) analysis of the enriched biological process (BP) (a), cellular component (CC) (b) and molecular function (MF) (c) for chloroquine targets identified by CQP-based ABPP. Fig. S4 GO enrichment analysis of biological process (BP) (a), cellular component (CC) (b) and molecular function (MF) (c) for chloroquine targets identified by MS-CETSA.

Additional file 3: Table S1. Antibodies used for Western blotting validation. Table S2 Target proteins identified by CQP-based ABPP. Table S3 Potential hits identified by MS-CETSA.

The synthesis of graphene-like carbon nitride has been a new research hotspot in the field of two-dimensional functional materials due to its similar structural characteristics to graphene and outstanding properties covering photocatalysis and lubrication and so on. Herein, the research progress of the microwave irradiation synthesis of graphene-like carbon nitride was discussed. Based on the comparison to the traditional preparation approaches including high-temperature oxidation corrosion, liquid phase ultrasonic exfoliation and thermal polymerization, etc., the advantages of microwave irradiation synthesis were analyzed. At the same time, one can find that by means of using high-energy microwave instrument and microwave absorbents covering graphite powders and short carbon fibers that possess excellent microwave energy absorption ability, the transferring and absorption efficient of energy as well as the non-steady degree of the synthesis reactions occurred in the microwave electromagnetic field can be enhanced, is consequently beneficial to the achievement of novel products with special morphology and structure.

Microwave synthesis has many advantages covering rapid, high-efficient, environmentally-friendly etc. Herein, graphene-like carbon nitride nanosheets (g-C3N4-NS) were successfully prepared by high-energy microwave heating method using melamine and carbon fibers as precursor and microwave absorber, respectively. The as-synthesized samples were investigated via various analytic techniques including X-ray diffraction (XRD), field-emission scanning electron microscope (FE-SEM), transmission electron microscopy (TEM), atomic force microscopy (AFM) and fourier transform infrared spectroscopy (FT-IR). Results show that the g-C3N4-NS sample prepared by microwave heating exhibits the obvious feature of graphene-like ultra-thin nanosheets in comparison with sample synthesized by conventional thermal polycondensation. Meanwhile, compared with graphene-like carbon nitride nanosheets prepared by other approaches including ultrasonic exfoliation and oxidation etching methods, the sample synthesized by microwave heating has smooth, flat and strong rigidity surface.

In this paper, a quad-slits interferometer using visible light is designed to measure the transverse beam size of Hefei Light Source-II (HLS-II). According to the beam parameters of the B7 source point, the preliminary simulation results are obtained by using the Synchrotron Radiation Workshop (SRW) code. Furthermore, the core parameters of the quad-slits components in the interferometer are optimized. Among, the optimum slits-separations of dH and dV are acquired to be 6.0 mm and 10.0 mm, respectively. It is shown that the simulated results are consistent with the theoretical values, which provides a reference value for performing the related experiments in the future., Proceedings of the 10th International Beam Instrumentation Conference, IBIC2021, Pohang, Rep. of Korea

In the previous experimental investigations and measurements using the radio-frequency (RF) phase modulation method to study the longitudinal beam characteristics of the Hefei Light Source-II (HLS-II), we found that the longitudinal bunch distributions under different modulation frequencies and amplitudes have great difference. In order to further explore island phenomenon and better understand beam motion associated with external RF phase modulation, the streak camera is exploited to effectively observe the longitudinal bunch profile and distribution in single-bunch filling mode. In addition, the dependence between island size, bunch dilution effect and modulation frequency are also discussed in detail. This is meaningful for researching the impact of RF noise on longitudinal beam dynamics, beam manipulation, and machine maintenance and debugging., Proceedings of the 10th International Beam Instrumentation Conference, IBIC2021, Pohang, Rep. of Korea

In this paper, the radio-frequency (RF) phase modulation method is exploited to investigate the variations in the transverse beam size and emittance at Hefei Light Source (HLS-II). Meanwhile, a certain quantitative analysis was performed on the stability and practicability of the beam transverse profile measurement systems. The experiments show that the RF phase modulation method can effectively explore the robustness and stability of beam transverse profile measurement systems over the range of 20.0-22.5 kHz, which is close to the first-harmonic of the synchrotron frequency. It is concluded that when the modulation amplitude of the external phase perturbation is less than 0.04 rad, this optical system can be capable of maintaining reliable and stable working status. This is also useful for analyzing the influence of RF phase noise on the subsequent beam measurement and diagnostics, which including the deterioration of beam quality, emittance blowup, beam jitter, and beam loss., Proceedings of the 9th International Beam Instrumentation Conference, IBIC2020, Santos, Brazil

Paget’s disease (PD) is an intraepithelial growth of neoplastic cells showing glandular differentiation. Primary esophageal PD is extremely rare, with only 14 cases reported to date. We report a case of esophageal PD in a 63-year-old man presenting with progressive dysphagia. On gross examination, the esophageal mucosa had a slightly mottled appearance and felt slightly thickened and indurated. Microscopically, the atypical tumor cells were mostly located in middle to basal cell layers of the squamous epithelium. Some tumor cells were difficult to be distinguished from normal squamous epithelium. Some regions of the lesion showed full-thickness cellular atypia with mitotic figures, and some tumor cells invaded through the basement membrane into the lamina propria, mimicking a squamous cell carcinoma. Acantholytic regions were prominent in the epithelium, and some gland-like clefts were formed. One recurrent laryngeal nerve lymph node showed metastatic foci. Immunohistochemically, tumor cells were positive for cytokeratin (CK) 7, CK8/18, carcinoembryonic antigen (CEA) and Her-2, but negative for CK5/6, p63, S-100 protein and HMB45, yielding the diagnosis of PD. This is the first case report of esophageal invasive Paget’s disease (invPD) and the first case report of esophageal acantholytic anaplastic Paget’s disease (AAPD).

An Infrared Free Electron Laser Light (IRFEL) is being constructed at National Synchrotron Radiation Laboratory. The EPICS Archiver Appliance provides the functions of historical data acquisition, archiving, migration, retrieval and management in the IRFEL facility. A Single-Page Web Application is developed for the data visualization based on Vue.js framework and Highcharts JavaScirpt library. A unified interface is developed for the visualization to integrate multiple data sources and provide the same retrieval entry of the historical data from EPICS Archiver Appliance, the real-time data from EPICS IOC, the statistical data from database and the alarm information from the Phoebus. This paper will describe the implementation details of data archiving and visualization of IRFEL., Proceedings of the 12th Int. Workshop on Emerging Technologies and Scientific Facilities Controls, PCaPAC2018, Hsinchu, Taiwan

The current historical data query and analysis system for the Hefei Light Source II (HLS-II) was developed with Apache Struts2. However, Apache Struts2 need to be fixed from time to time to avoid being attacked. Therefore, a new system based on Spring Boot and Vue.js is developed. Meanwhile, the performance of the system is optimized, and the radiation monitor module is added. This paper will detail the system architecture and software implementation., Proceedings of the 10th Int. Particle Accelerator Conf., IPAC2019, Melbourne, Australia

A data warehouse based on Hadoop ecosystem is designed and constructed for Hefei Light Source II (HLS-II). The ETL program based on Spark migrates data to HDFS from RDB Channel Archiver and the EPICS Archiver Appliance continuously and store them in Parquet format. The distributed data analysis engine based on Impala greatly improves the performance of data retrieval and reduces the response time of queries. In this paper, we will describe our efforts and experience to use various open sources software and tools to effectively manage the big data. We will also report the plans on this data warehouse in the future., Proceedings of the 12th Int. Workshop on Emerging Technologies and Scientific Facilities Controls, PCaPAC2018, Hsinchu, Taiwan

Short electron bunch has interesting applications in the synchrotron radiation light sources, such as the production of powerful coherent THz radiation, time resolving spectrum analysis, etc. In this work, we are interested in acquiring the short bunch in the storage ring with a small circumference like Hefei Light Source. In this paper, we tried to approach the short bunch in two separate methods: by increasing the higher harmonic cavity voltage and by reducing the momentum compaction factor. The preliminary result and observations are shown and discussed., Proceedings of the 10th Int. Particle Accelerator Conf., IPAC2019, Melbourne, Australia

An InfraRed Free Electron Laser Light (IRFEL) is under commissioning at National Synchrotron Radiation Laboratory (NSRL). The control system of IRFEL is a distributed system based on Experimental Physics and Industrial Control System (EPICS). The alarm system is an essential part of the control system. It is developed based on the software Phoebus. The module named "Alarms" in Phoebus can store states and configuration information of the Process Variable (PV) in the Kafka topics. To meet our requirements, 3 kinds of alarm message distribution applications are developed, i.e. Web-Based GUI, WeChat and SMS. This paper will introduce the alarm system architecture and the implementations of the applications for alarm message distribution., Proceedings of the 10th Int. Particle Accelerator Conf., IPAC2019, Melbourne, Australia

The FELiChEM is a new infrared free electron laser (IR-FEL) facility, which is being built in the National Synchrotron Radiation Laboratory (NSRL) in Heifei, China. The facility will provide continuously tunable pulsed laser radiation covering the mid-infrared (MIR) wavelength range from 2.5 to 50μm and the far-infrared (FIR) range from 40 to 200μm. The output macro pulsed laser width is 5-10μs and pulsed laser power is 2-10kW. In order to evaluate pulsed laser saturation time and FEL optical cavity losses, the rise time and fall time of macro pulsed laser need to be measured. Laser intensity measurement system for the FELiChEM is being designed. This system is composed of optical system, pyroelectric detector and electronics. Each module will be described in detail in this paper. The laser intensity measurement system was tested under offline and online conditions. The results showed that pulsed laser of 10μs width can be measured and the design requirement can be met with this system., Proceedings of the 9th Int. Particle Accelerator Conf., IPAC2018, Vancouver, BC, Canada

The Hefei Light Source II (HLS-II) is a vacuum ultravi-olet (VUV) synchrotron light source. A major upgrade of the light source was finished in 2014, and the timing system was rebuilt with event-system to meet synchroni-zation requirements of the machine. The new timing system provides about 100 output signals with various interfaces. The time resolution of this system is 9.8 ns for most devices and 9 ps for the electron gun and the injec-tion kickers. The measured jitter of the output signal is less than 27 ps (RMS). In order to improve the perfor-mance of light source, the top-off operation mode has been planned. As part of this plan, both the hardware and the software of the timing system are upgraded. By ob-taining real-time data of beam measurement of storage ring, the automatic selection of the bucket is implement-ed. With any designated bunch pattern, top-off injection is achieved, and the storage ring beam can be uniform filled well., Proceedings of the 16th Int. Conf. on Accelerator and Large Experimental Control Systems, ICALEPCS2017, Barcelona, Spain

The HLS-II storage ring is a crucial part of Hefei Light Source. Tune is one of the most important parameters of the electron storage ring, of which the tune measurement system is an integral component. In this paper, the design of a new tune measurement system based on BBQ (base band tune), is presented. Some experiments are performed to test this system. The new system is compared with the original system and the TBT (turn-by-turn) method respectively. The obtained results illustrate higher accuracy and higher stability for the new system. A new approach of calculating the betatron oscillation amplitude is proposed, and the betatron oscillation amplitudes in the normal running stage for the HLS-II storage ring are estimated at 95 nm (horizontal) and 60 nm (vertical)., Proceedings of the 9th Int. Particle Accelerator Conf., IPAC2018, Vancouver, BC, Canada

High-energy vacuum microwave irradiation was directly applied to synthesize silicon carbide (SiC). Results indicated that the well-crystallized β-SiC grains can be obtained rapidly and conveniently via microwave approach, even simply constituents covering silica dioxide powders and artificial graphite were employed as raw materials. Based on the comprehensive micro-structural characterizations, the formation of β-SiC, under high-energy vacuum microwave irradiation, could be properly explained by the mechanism of two-dimension nucleation and growth. Be- sides, the technology of electron backscattered diffraction (EBSD) was particularly used to characterize the crystal plane of the observed SiC grains. The corresponding calculation of Euler angles suggested that the fastest-growing crystal planes {211} should be overlapped gradually, and the evolution from {211} to {220} crystal planes should be realized through the formation of {421} transformation plane. The most stable crystal planes of {111} became the regular hexagonal planes in the end, which can be explained by the Bravis rule. Correspondingly, the {220} crystal planes were finally existed as the side-planes of SiC grains. The detailed EBSD investigation provided the experimen- tal evidences for the evolution of crystal planes in the growth process.

The real-tine and accurate knowledge of the operation of an atomic clock is of great significance to satellite navigation and time keeping. In the light of the problems which exist in the real-time solution of the atomic clock operating parameters by means of the standard Kalman filtering, the method for applying the adaptive Kalman filtering model based on the prediction residual to the real-time solution of the clock parameters is proposed. An adaptive factor is constructed by the model based on the standard Kalman filtering model so that the accurate and reliable estimates of the operating parameters may be given rather fast after the frequency modulation or phase modulation of the atomic clock. Finally, in combination with the 3 computational examples of the work on the navigation and time keeping, the effectiveness of the method is verified.

The prediction of the clock errors of atomic clocks plays an important role in the work on time and frequency. Each of the prediction models often used at present has its own merits and shortages. A combination of the predicted results obtained by means of these models can be used to synthesize the characteristics of various kinds of prediction models. In the light of the problem which occurs when the linear combination model is used to make the prediction of clock errors, the concept of learning weight is proposed and the modified combination prediction model is made by taking advantage of various kinds of pieces of accuracy information. For verifying the efficiency of this method the clock error sequences of the IGS (International GNSS Service) of 4 GPS satellites are selected and the predicted results of the quadratic polynomial and grey model are combined. The result shows that the modified model can further improve the stability and accuracy based on the guarantee of the reliability.

Cardiac myxomas are the most common primary neoplasms of heart. The present study was performed on the 61 cases of patients with cardiac myxoma, in order to investigate the tumors' clinical and pathological features, and to identify the relationship between the pathological characteristics and clinical behaviors.A total of 61 cardiac myxoma cases were analyzed and reviewed retrospectively, including the clinical presentations, physical examinations, and echocardiography, electrocardiography, and pathology documents.The total patient cohort was made up of 37 women and 24 men. The average age at diagnosis was 48.8 years in males and 51.9 years in females. The most common complaint was dyspnea (37 cases, 60.7%) and the most common sign was systolic murmur (30 cases, 49.2%). Two surface structures and three tumor cell arrangement patterns were observed, and statistical analysis revealed the surface structure was related to the cell arrangement pattern. However, neither the cell arrangement pattern nor the tumor surface structure showed a significant correlation with the clinical presentation.The present study showed the pathological profiles of cardiac myxomas were not related to the clinical presentations. The results of our study indicate morphologic classifications of cardiac myxomas may not be significant for clinical practice.

In geographical information engineering, height anomalies must be known in order to convert GPS ellipsoid heights into geodetic heights. There are many conversion models, such as polynomial, BP neural network and multi-quadrics fitting. Because the quasi-geoid is an irregular geometric object, every method has both advantages and disadvantages, and is appropriate to different conversion patterns. It is difficult to identify which conversion model is most suitable for a particular area. In order to obtain a more precise and reliable analytical result, the combined model based on least squares support vector machines (LSSVM) has been approached. Derived from statistical learning theory, LSSVM are learning systems that use a hypothesis space of linear function in a high dimensional feature space, trained with a learning algorithm from optimizations theory. As a result, the fitting ability of single models is significantly improved. The combined model still possesses the same important features as the single models. Examples are presented and the results are analyzed in detail to demonstrate the efficiency of the proposed methodology.