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Additional file 1. Percentage of reads number with different lengths at three reproductive stages of sheep.

Additional file 5. The expression level of target genes for DE miRNAs between anestrus and breeding season in different reproductive stages.

Additional file 7. Highly expressed genes during anestrus or breeding season in pineal gland of sheep.

Additional file 9 Stem-loop primers employed for RT-PCR of miRNAs and primers employed for Q-PCR of AANAT and miRNAs.

Additional file 8. Predicted miRNAs potentially targeting genes in ribosome pathway.

Additional file 10. Mature sequences of miRNAs and inserted sequences in the pcDNA6.2-GW/EmGFP-miR vector.

Additional file 3. The signaling pathways which the stage-specific expressed miRNAs were predominantly enriched in.

Additional file 9 Stem-loop primers employed for RT-PCR of miRNAs and primers employed for Q-PCR of AANAT and miRNAs.

Additional file 7. Highly expressed genes during anestrus or breeding season in pineal gland of sheep.

Additional file 8. Predicted miRNAs potentially targeting genes in ribosome pathway.

Additional file 4 The genes number and P value of pathways which target genes for differentially expressed miRNAs between anestrus and breeding season were enriched in.

Additional file 5. The expression level of target genes for DE miRNAs between anestrus and breeding season in different reproductive stages.

Additional file 2. Expression information of small RNA reads in ovine pineal gland.

Objective: To evaluate the safety, cosmesis, and clinical outcome of intraoperative electron radiation therapy (IOERT) delivered prior to lumpectomy for early-stage breast cancer. Methods: From December 2008 to March 2012, 75 breast cancer patients (ages 34-66 years) were treated with IOERT during breast conservative surgery. IOERT was delivered using a mobile linear accelerator. Suitable energy and applicator size were chosen to ensure coverage of the tumor with anterior and posterior margins of 1 cm and lateral margins of 2 cm. Patients with sentinel node metastases or younger than 40 years received 8 Gy as boost followed by post-operative external beam radiation therapy of 50 Gy/25F; the others had 15 Gy, prescribed to the 90% isodose depth. Adjuvant treatment consisted of chemotherapy (55 patients), hormonal therapy (59 patients), or combined chemotherapy and hormonal therapy (41 patients). The safety, cosmesis, and short-term outcome were evaluated. Results: Median follow-up was 54 months (range: 30-66 months). Two (2.7%) patients developed post-surgical hematoma. Six (8.0%) patients developed mild breast fibrosis. Eight (10.7%) patients suffered from local pain. One (1.2%) patient experienced a post-operative infection. Sixteen (21.3%) patients developed Grade 1 pulmonary fibrosis. Forty-three (57.3%) patients had an excellent cosmetic result and 23 (30.7%) had a good cosmetic result. Three patients had an ipsilateral breast recurrence, with an actual 3-year local recurrence rate of 4.0%. One patient had an ipsilateral axillary recurrence, resulting in a 3-year regional recurrence rate of 1.3%. No distant metastases or deaths were observed. The 3-year disease free survival was 94.6%. Conclusions: Intraoperative electron radiation therapy delivered prior to lumpectomy is safe and feasible for selected patients with early-stage breast cancer. Early side effects, cosmesis and short-term efficacy are acceptable, but a longer follow-up is needed for evaluation of late side effects and long-term outcome.

Primers used for real time-PCR. (DOC 39Â kb)

Based on the tree-ring growth characteristics of Erman's birch (Betula ermanii charm.) and the relationships between it and climatic factors at elevation of 1950m, the sensitivity of tree lines in Changbai Mountain to climatic factors was assessed. The results indicated tree line forest in Changbai Mountain had an obvious, sensitivity to climate factors. However, difference from other study sits is that the main climatic control factor on tree-ring growth was not current growth season temperatures, as might be expected, but previous winter and current March temperature. Although the precipitation in the region was quite abundant, the tree-ring growth was still significantly correlated with the precipitation during previous winter and current spring. Additionally, climatic factors which influenced the Erman's birch growth were not the yearly variables, but seasonal and monthly variables. Therefore the reported increase in yearly mean temperature and total yearly precipitation since 1980s was not responded by sustained increase in ring widths in recent decades.

Two-dimensional vector vortex solitons in harmonic optical lattices are investigated. The stability properties of such solitons are closely connected to the lattice depth V0. For small V0, vector vortex solitons with the total zero-angular momentum are more stable than those with the total nonzero-angular momentum, while for large V0, this case is inversed. If V0 is large enough, both the types of such solitons are stable.

Papillary renal cell carcinoma (PRCC) is traditionally classified into type 1 and type 2. Recently, an oncocytic variant of PRCC has been described. We report a series of 6 oncocytic renal papillary tumors (OPRCC) which tended to occur in older patients (mean, 56.8 years) with a male preference (male-to-female ratio is 5:1). All 6 patients are alive with no evidence of disease after initial resection, showing an indolent clinical behavior. Histologically, tumors exhibited predominant papillary structure with delicate fibrovascular cores. Papillae were lined by single layers of cells with large, deeply eosinophilic and finely granular cytoplasms and round regular nucleus. The phagocytosis of tumor cells was frequently and evidently seen in our cases that hemosiderin-laden tumor cells and foamy tumor cells were noticed in five and four cases respectively. All tumors were immunoreactive for racemase, vimentin, CD10, and MET and negative for CD117. While E-cadherin, EMA, and cytokeratin 7 exhibited variable immunopositivity. FISH analysis was performed in five of six cases and found heterogeneous results. Trisomy of chromosomes 7 was found in three cases and trisomy of chromosomes 17 in two cases. Loss of chromosome Y was noted in one of four tumors in male patients. MET gene status was also investigated by direct sequencing in all 6 cases and found no distinct mutation in any case. These results suggest that OPRCC shows distinct morphology, indolent clinical behavior, and similar immunohistochemical and cytogenetic features with PRCC, seems to be a variant in the PRCC group. Whether the strong expression of MET indicates a potential therapeutic target is still unknown and requires further investigation in clinical trials.

We propose an experimental setup to measure the threshold pressure gradient (TPG) of microchannels with different wettability surfaces by the static method in microchannels with diameters from 20 μm to 320 μm, and compare the TPG of microchannels with adsorption of cetyl trimethyl ammonium bromide (CTAB) with that without CTAB adsorption. The results show the existence of TPG in microchannels. The TPG of microchannels increases with decreasing hydrodynamic diameter, and the relation between TPG and diameter is in agreement with the single-log normalization. The TPG of a microchannel with CTAB adsorption decreases obviously as compared with the microchannel without CTAB adsorption. The TPG of microchannels with different wettabilities of boundary surface are different, and the resistance of liquid flow can be reduced by changing the wettability of boundary surfaces.

We demonstrate that a new class of stable composite soliton exists in a two-dimensional lattice. It can be produced by the evolution of a stationary solution of nonlinear Schrdinger equation with a periodic potential modulation. We emphasize that this new kind of composite soliton is different from the dipole soliton reported before though they seem alike. The components of the soliton exchange energy during the propagations and the total energy of soliton conserves.

The interaction between two optical vortices in a linear medium is investigated numerically. An instability resembling the symmetrybreaking instability occurs when the vortices carry opposite topological charges. The screw dislocations wi ll evolve into another type of dislocationedge dislocation with the propagatin g of optical field. The sum of the topological charges conserves in the whole pr ocess.