1. The complete nucleotide sequence of region 1 of the CFA/I fimbria! operon of human enterotoxigenicEscherichia coli
- Author
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Willshaw Ga, Bart J.A.M. Jordi, van der Zeijst Ba, and Wim Gaastra
- Subjects
DNA, Bacterial ,Operon ,Molecular Sequence Data ,Biology ,medicine.disease_cause ,Biochemistry ,Enterotoxins ,Open Reading Frames ,Endocrinology ,Plasmid ,Enterotoxigenic Escherichia coli ,Escherichia coli ,Genetics ,medicine ,Humans ,Amino Acid Sequence ,Codon ,Molecular Biology ,Gene ,Antigens, Bacterial ,Base Composition ,Base Sequence ,Structural gene ,Nucleic acid sequence ,Molecular biology ,Fimbriae, Bacterial ,Codon usage bias ,Fimbriae Proteins - Abstract
The production of the plasmid-encoded fimbrial antigen CFA/I of enterotoxigenic Escherichia coli requires two DNA regions: CFA/I region 1 and CFA/I region 2. These two regions are separated by about 40 kb on the wildtype plasmid. CFA/I region 1 contains the structural genes, whereas CFA/I region 2 contains a positive regulator. The first two genes (cfaA and cfaB) and the cfaD' sequence of region 1 have already been described. Here the total nucleotide sequence of region 1 is presented. Two new genes in region 1 are described, named cfaC and cfaE. The GC content of the genes in region 1 is 33.6% which is substantially lower than normally found in E. coli genes (50%). The codon usage also differs from the standard codons used in E. coli.
- Published
- 1992
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