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Supplementary Material 2: The X-tile software determined the optimal cutoff point of LNR. LNR: lymph node ratio

Yu Li,1,* Peng Wang,1,* Lei-Lei Wu,2 Jun Yan,1 Xue-Ying Pang,1 Song-Jiang Liu1 1Department of Oncology, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin 150040, Heilongjiang Province, People’s Republic of China; 2School of Pharmaceutical Sciences, Mudanjiang Medical University, Mudanjiang 157011, Heilongjiang Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Song-Jiang LiuDepartment of Oncology, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, No. 26, Heping Road, Harbin 150040, Heilongjiang Province, People’s Republic of ChinaTel +86-13796228787Email 17092140096@163.comPurpose: Gastric cancer (GC) is a malignant disease of digestive tract. Clinically, radiation therapy is widely applied in treating GC, while with undesirable outcome due to tumor re-proliferation and recurrence and metastasis after radiation. Therefore, it is crucial to explore potential molecular mechanisms to develop therapeutic strategies. The present study found that miR-26a-5p has low expression in GC patients and could regulate Wnt5a to inhibit tumor growth, which was a potential therapeutic target for GC. To explore the expression and related mechanism of miR-26a-5p and Wnt5a in GC.Patients and Methods: MiR-26a-5p and Wnt5a were extracted from the transcriptome data of GC downloaded from TCGA database for analysis. The expression levels of miR-26a-5p and Wnt5a in patients’ tissues and serum were detected by qRT-PCR, and their correlation with patients’ pathological data and survival was analyzed. In addition, miR-26a-5p and Wnt5a overexpression and inhibition vectors were transfected into cells to observe the effects on the proliferation, invasion and apoptosis of GC cells. The relationship between miR-26a-5p and Wnt5a was analyzed by dual luciferase report.Results: The database and clinical samples showed that miR-26a-5p level was low while Wnt5a was high inGC. MiR-26a-5p level decreased in patients with stage III+IV, lymphatic metastasis and tumor ≥ 3cm, and Wnt5a was contrary to that of the miR-26a-5p, with diagnostic value. Overexpressed miR-26a-5p and inhibited Wnt5a enhanced apoptosis, decreased proliferation and invasion, reduced Bcl-2 and β-catenin proteins, and elevated Caspase 3, E-cadherin and Bax proteins, while inhibited miR-26a-5p and over-expressed Wnt5a showed the opposite results. Dual luciferase report confirmed that miR-26a-5p targeted to regulate Wnt5a, and rescue experiments found that these effects could be counteracted by reducing miR-26a-5p level.Conclusion: Overexpressed miR-26a-5p can inhibit Wnt5a expression, promote cell apoptosis, and suppress cell proliferation and invasion in GC.Keywords: TCGA, miR-26a-5p, Wnt5a, gastric cancer, survival, diagnosis

The purpose of this research paper to identify the impact of sustainable tourism management towards the economic conditions of a community. In 2018, Pandawa Beach has won first rank for Indonesia Sustainable Tourism Awards (ISTA) in the field of economic utilization to local communities hence the author decided to use Pandawa Beach for the research. The qualitative data were collected from interviews and the quantitative data were collected from questionnaires. The method for analyzing the data is using descriptive analysis. The research result shows that sustainable tourism gives positive impacts towards the economic conditions on Kutuh Village. 72 people out of 90 are satisfied with the current economic welfare of the local community. The data is also valid because the AVE and CR results are all above 0.7 which means the results are acceptable. Keywords: sustainable tourism management, economic impact of tourism, Pandawa Beach Bali