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In previous works we observed that the administration of a high-protein diet (HPD) to rats and mice, attenuated the deleterious effect of streptozotocin (SZ) on insulin secretion (IS). On the other hand transfer of splenocytes (S) from diabetic to normal mice, caused in the last ones, glucose-intolerance and a significant diminution in glucose-stimulated IS. In the present work we studied if the administration of HED to mice that had received diabetic S, modify the observed alteration in IS. C57BL/6J mice were injected with 5 doses of SZ (40 mg/Kg/day). 15 days later their spleens were dissected and S isolated. These “diabetic” S were injected i.p. in receptor mice kept on a control diet (CD) or HPD. 15 days after S transfer, pancreatic gland from receptor mice were perifused and IS patterns were evaluated. Results showed that mice transferred with “diabetic” S, kept either on CD or HED presented diminished 1st. and 2nd. phases of IS (CD: 1st. phase: 1288.7±40.4, n=6 vs 993.4±16.9 uU/6 min/100 mg wt.,n=6, p < 0.001; 2nd phase:117610±201.9, n=6 vs 10712±220.5 uU/32 min/100 mg wt, n=5, p < 0.01; HPD: 1st. phase: 1322.6±31.2, n=5, vs 1134.0±15.4, n=9, p < 0.01; 2nd phase: 11867±295.6vs 10628±224.6, n=5 and 9, p < 0.01). However in mice fed HPD and transferred with “diabetic” S, 1st.phase of IS was significantly higher than values from mice under CD (p < 0.001). These results suggest that HPD improved the B-oell secretory response in an experimental model of diabetes with anti beta cell imrune aggression.

The aim of this chapter is to show the key features of adult colon stem cells and provide a useful tool for their isolation, characterization and propagation.