1. Effect of electroacupuncture on calcium-activated chloride channel currents in interstitial cells of Cajal in rats with diabetic gastroparesis
- Author
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Chen Hai-jiao, Yang Jian-wen, Wei Xing, Peng Yan, Zhang Cheng-cheng, Cao Jian-zhong, and Lin Ya-ping
- Subjects
medicine.medical_specialty ,business.industry ,Electroacupuncture ,medicine.medical_treatment ,Intraperitoneal injection ,0211 other engineering and technologies ,02 engineering and technology ,Zusanli ,Streptozotocin ,Group A ,Group B ,030205 complementary & alternative medicine ,Interstitial cell of Cajal ,03 medical and health sciences ,symbols.namesake ,0302 clinical medicine ,Endocrinology ,Complementary and alternative medicine ,Group (periodic table) ,Internal medicine ,021105 building & construction ,symbols ,Medicine ,business ,medicine.drug - Abstract
To investigate the mechanisms of electroacupuncture (EA) at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6) in intervening diabetic gastroparesis (DGP) based on calcium-activated chloride channel. Forty Sprague-Dawley rats were randomly divided into four groups, including a normal control group (group A), a model group (group B), an EA group (group C) and a metoclopramide group (group D), with 10 rats in each group. A single intraperitoneal injection of 2% streptozotocin (STZ) combined with 8-week high-glucose high-fat diet was used to establish a DGP rat model. After intervention, gastrointestinal propulsive rate was observed; the expression level of transmembrane protein 16A (TMEM16A) was examined by immunohistochemistry; the Ca2+ concentration in interstitial cells of Cajal (ICCs) was detected by immunofluorescence; and whole-cell patch-clamp technique was applied to detect the current intensity of calcium-activated chloride channel (ICaCC) in ICCs in gastric antrum. After modeling, the blood glucose levels in group B, group C and group D were significantly increased compared with group A (all P
- Published
- 2021
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