1. HPV18 E7 induces the over-transcription of eIF4E gene in cervical cancer
- Author
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Pang, Tianyun, Wang, Sen, Gao, Min, Kang, Haixian, Zhao, Yi, Yao, Yunhong, and Hu, Xinrong
- Subjects
HPV ,EIF4E ,lcsh:R ,Cervical cancer ,lcsh:Medicine ,Original Article ,E7 - Abstract
Objective(s): Eukaryotic translation initiation factor 4E (eIF4E) is overexpressed in cervical cancer (CC). However, the molecular mechanisms are unclear. This study aimed to investigate the molecular mechanism of eIF4E gene overexpression in CC. Materials and Methods: The human papillomavirus (HPV) type 18 E7 and eIF4E mRNAs were measured following knock down or overexpression of E7 gene by RT-PCR and real-time PCR. Cell counting kit-8 assay was used to determine the cell proliferation. Flow cytometry was used to analyze the cell cycle and apoptosis. Transwell system was employed to determine the cell migration. Results: Overexpression of E7 gene increased eIF4E mRNA level by 24.3% (P
- Published
- 2015