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In dry land areas of the western half of Iran, chickpea due to exposure to rotation with wheat and barley play an important role in maintaining survival of agriculture in these regions. Seed priming is a simple and cheap method and is highly efficient and acceptable, especially in areas with low fertility. In this study, effects of different times of hydropriming on yield, yield components, phenological characteristics and percentage of protein of chickpea (Cicer arietinum L.) were examined in a randomized complete block design with three replicates in 2010. Seeds of chickpea were exposed at six different hydropriming times (2 h, 4 h, 6 h, 8 h, 10 h and control). The results of this experiment showed that the effect of hydropriming treatments for main branch and lateral branch number, number of pod per plant, biological yield, grain yield, time from planting to emergence, emergence to flowering, flowering to bloom and pod forming and growth length was significant. However, there was no significant difference between treatments in terms of plant height, number of seed per pod, number of empty pod, seed thousand weight, harvesting index, pod forming to seed pods and blooming to maturity, and percentage of seed protein.Key words: Chickpea, yield, phenological characteristics, hydropriming.

Proteomics is a powerful technique to study proteomes extracted from biological sources. Proteome analysis classically is based on two-dimensional electrophoresis (2D-E) for protein separation and mass spectrometry (MS) for the protein identification. The serum protein analysis is a useful diagnosis that can be used as an indicator of the physiological or clinical status of a patient. One of the central and critical steps is sample application, therefore this method should be considered and optimized for 2-DE with immobilized pH gradient. The aim of this study was focused on comparative application of ingel (rehydration loading) and in-cup (cup loading) sample application for 2-DE of human serum analysis. The results indicate that the number of spots detected with the rehydration loading was higher than the number obtained by cup-loading sample application. Also in this work, in order to obtain the best result with high resolution, a 2-DE procedure was optimized.Key words: Proteomics, 2D-E, proteome, sample application.

The Thirteenth East Asia-Pacific Conference on Structural Engineering and Construction (EASEC-13), September 11-13, 2013, Sapporo, Japan.

The Helicobacter pylori use a number of mechanisms to survive in the stomach lumen and can lead to gastritis and reduction in stomach acid secretion. It has been found that the risk of developing gastric carcinoma is associated to heterogeneity of H. pylori virulence factors such as HopQ. The HopQ is one of the outer membrane proteins involved in bacterial adherence to gastric mucosa and has been suggested to also main role in the virulence of H. pylori. The purpose of the current study was to investigate the association between different H. pylori virulence hopQI (types I) genotyping and patients with gastroduodenal disorders. For this purpose 58 stomach biopsies of the patients with gastric cancer and 100 saliva samples from healthy and H. pylori infected individuals were collected and studied. Then genomic DNA was purified and PCR was done for desired gene via specific primers. The H. pylori infections were diagnosed using PCR for GlmM gene. Then frequencies of hopQI+ and hopQI- genotypes were determined in H. pylori infected cases. Statistical analysis showed that there were not significant differences between healthy and diseased ones for genotypes hopQI+ and hopQI-. Then the hopQI+ cannot be as a risk factor genotype for gastric cancer.

Periodontal disease is one of the most prevalent inflammatory illnesses and is a main cause of tooth loss in human population. Tumor necrosis factor-α (TNF-α) gene is one of pro-inflammatory cytokines which has important role in pathogenesis of periodontal disease. The main purpose of this study is to determine genotype abundance of TNF-α-1031 gene in both groups of patients and controls, and also investigation of relation of single nucleotide polymorphism (SNP) these genotypes with periodontal disease risk. DNA was extracted from blood tissue of 31 patients and 54 controls. The TNF-α-1031 polymorphism was evaluated by polymerase chain reaction- confronting two-pair primer (PCR-CTPP) method. In the GAP group, the frequencies of TT, TC and CC genotypes were 35.48%, 61.29 and 3.23%, respectively. In controls the frequencies of TT, TC and CC genotypes were 22.22%, 72.22%, and 5.56%, respectively. Results of this study showed that there was no significant association between TNF-α (-1031 T/C promoter) gene polymorphisms and the risk of generalized aggressive periodontitis disease.

Generalized aggressive periodontitis (GAP) is a subtype of periodontal diseases that characterized by rapid destruction of periodontal supporting tissues. The MnSOD Val-9Ala mutation of manganese superoxide dismutase gene (MnSOD Val-9Ala) and its correlation with periodontal diseases has been studied in different populations. The purpose of this study was to investigate the possible association of MnSODVal-9Ala polymorphism with periodontitis disease in sample of GAP patients in Iran for the first time. Following a GAP examination, 50 GAP patients and 100 healthy individuals were recruited. Genomic DNA was extracted from peripheral blood leukocytes and the MnSODVal-9Ala polymorphismwas detected using PCR-RFLP method. The frequency of Ala/Ala, Ala/Val and Val/Val genotypes in healthy individuals were 25, 66 and 9%, respectively. In periodontitis patients, frequencies were as Ala/Ala (12%), Ala/Val (50%) and Val/Val (38%) genotypes. There was a significant positive association between distribution of MnSOD Val-9Ala genotypes and the risk of periodontitis disease (p0.05). Our results indicated that MnSOD Val-9Ala gene polymorphism has a positive association with the risk of periodontitis disease.