263 results on '"Yuehua Wang"'
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2. Lonicera caerulea polyphenols inhibit fat absorption by regulating Nrf2-ARE pathway mediated epithelial barrier dysfunction and special microbiota
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Yuehua Wang, Ningxuan Gao, Andrea Nieto-Veloza, Lingxi Zhou, Xiyun Sun, Xu Si, Jinlong Tian, Yang Lin, Xinyao Jiao, and Bin Li
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Food Science - Published
- 2023
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3. Predictive value of suicidal risk assessment using data from China's largest suicide prevention hotline
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Yongsheng Tong, Yi Yin, Kenneth R. Conner, Liting Zhao, Yuehua Wang, Xuelian Wang, and Yeates Conwell
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Psychiatry and Mental health ,Clinical Psychology - Published
- 2023
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4. DESIGN AND EXPERIMENT OF COMB-BRUSH AND AIR-SUCTION COMPOUND TYPE FLAX CAPSULE HARVESTING TEST BENCH
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Fulong XU, Junlin HE, Yuehua WANG, and Jiaojiao LI
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Mechanical Engineering ,Industrial and Manufacturing Engineering ,Food Science - Abstract
In view of the problem of plant entanglement threshing roller in the process of mechanized flax harvesting, a comb-brush air-suction composite flax capsule harvesting test stand was designed based on the harvesting method of harvesting capsules independently and then breaking the capsules to thresh them. The following four parameters were selected as experimental factors: the shape of comb tooth cross-section, the rotational speed of comb roller, the relative speed of machine travel and the air volume of centrifugal fan. The following four indicators were chosen as the experimental indicators: capsule removal rate, capsule breakage rate, capsule collection rate and plant winding rate. A four-factor and three-level orthogonal test was conducted. The results showed that the optimal combination of test factors was as follows: the shape of comb tooth cross-section was rectangular, the rotational speed of the brush roller was 90 rad/min, the speed of machine travel relative to the plant was 80 mm/s and the air volume of centrifugal fan was 6000 m3/h. Under the condition of better experiment parameters combination, the capsule removal rate was 96.45%, capsule shell breaking rate was 98.79%, capsule collection rate was 95.65% and flax plants winding rate was 2.52%. The comb-brush air-suction composite flax capsule harvesting test bench provided the feasibility scheme for the problem of plant winding thresher roller and capsule collection. more...
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- 2022
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5. In vitro and in vivo activity of meropenem+avibactam against MBL-producing carbapenem-resistant Klebsiella pneumoniae
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Xiuyun Li, Zhaowen Chen, Jin Jiao, Shifu Wang, Yuehua Wang, Weiwei Wu, Huijun Yang, and Hongxiang Lou
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Microbiology (medical) ,Infectious Diseases ,Virology ,Microbiology - Published
- 2022
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6. Preparation and Performance of Regenerated Al2O3-Coated Cathode Material LiNi0.8Co0.15Al0.05O2 from Spent Power Lithium-Ion Batteries
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Xi, Liwen Ma, Guangyun Liu, Yuehua Wang, and Xiaoli
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spent power lithium-ion battery ,solid phase sintering ,regeneration ,coated cathode materials - Abstract
In this study, LiNi0.8Co0.15Al0.05O2@x%Al2O3-coated cathode materials were regeneratively compounded by the solid-phase sintering method, and their structural characterization and electrochemical performance were systematically analyzed. The regenerated ternary cathode material precursor synthesized by the co-precipitation method was roasted with lithium carbonate at a molar ratio of 1:1.1, and then completely mixed with different contents of aluminum hydroxide. The combined materials were then sintered at 800 °C for 15 h to obtain the regenerated coated cathode material, LiNi0.8Co0.15Al0.05O2@x%Al2O3. The thermogravimetry analysis, phase composition, morphological characteristics, and other tests show that when the added content of aluminum hydroxide is 3%, the regenerated cathode material, LiNi0.8Co0.15Al0.05O2@1.5%Al2O3, exhibits the highest-order layered structure with Al2O3 coating. This material can better inhibit the production of Ni2+, and improve material structure and electrochemical properties. The first charge–discharge efficiency of the battery assembled with this regenerated cathode material is 97.4%, a 50-cycle capacity retention is 93.4%, and a 100-cycle capacity retention is 87.6%. The first charge–discharge efficiency is far better than that of the uncoated regenerated battery. more...
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- 2023
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7. Dietary Polyphenols Alleviate Autoimmune Liver Disease by Mediating the Intestinal Microenvironment: Challenges and Hopes
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Zhen Cheng, Yuehua Wang, and Bin Li
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Liver Diseases ,Dietary Supplements ,Humans ,Polyphenols ,General Chemistry ,General Agricultural and Biological Sciences ,Gastrointestinal Microbiome - Published
- 2022
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8. Chrysomycin a Regulates Proliferation and Apoptosis of Neuroglioma Cells via Akt/GSK-3β Signaling Pathway In Vivo and In Vitro
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Dongni Liu, Man Liu, Yufu Shang, Wenfang Zhang, Shanshan Zhang, Fuhang Song, Huawei Zhang, Guanhua Du, and Yuehua Wang
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Glioblastoma (GBM) is a major type of primary brain tumors without ideal prognosis and it’s necessary to find novel compound possessing therapeutic effect. Chrysomycin A (Chr-A) has been reported to inhibit the proliferation, migration and invasion of U251 and U87-MG cells, but the mechanism of Chr-A against glioblastoma in vivo and whether Chr-A modulates apoptosis of neuroglioma cells is unclear.The present study was to elucidate the potential of Chr-A against glioblastoma in vivo and how Chr-A modulates apoptosis of neuroglioma cells. Briefly, The an-ti-glioblastoma activity was assessed in human glioma U87 xenografts nude mice. Chr-A-related targets were identified by RNA-seq. Apoptotic ratio and caspase 3/7 activity of U251 and U87-MG cells were assayed by flow cytometry. Apoptosis-related proteins and possible molecular mecha-nism were validated via Western blotting. The results showed that Chr-A treatment significantly inhibits glioblastoma progression in xenografts nude mice, and enrichment analysis suggested that apoptosis, PI3K-Akt and Wnt signaling pathway were involved in the possible mechanism. Chr-A increased apoptotic ratio and the activity of caspase 3/7 in U251 and U87-MG cells. Western blot revealed that Chr-A broke the balance between Bax and Bcl-2 activating caspase cascade re-action and downregulated the expression of p-Akt and p-GSK-3β, suggesting that Chr-A may contribute to glioblastoma regression modulating Akt/GSK-3β signaling pathway to promote apoptosis of neuroglioma cells in vivo and in vitro. Therefore, Chr-A may hold therapeutic promise for glioblastoma. more...
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- 2023
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9. Biological characteristics and pathogenicity of Acanthamoeba
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Yuehua Wang, Linzhe Jiang, Yitong Zhao, Xiaohong Ju, Le Wang, Liang Jin, Ryan D. Fine, and Mingguang Li
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Microbiology (medical) ,Microbiology - Abstract
Acanthamoeba is an opportunistic protozoa, which exists widely in nature and is mainly distributed in soil and water. Acanthamoeba usually exists in two forms, trophozoites and cysts. The trophozoite stage is one of growth and reproduction while the cyst stage is characterized by cellular quiescence, commonly resulting in human infection, and the lack of effective monotherapy after initial infection leads to chronic disease. Acanthamoeba can infect several human body tissues such as the skin, cornea, conjunctiva, respiratory tract, and reproductive tract, especially when the tissue barriers are damaged. Furthermore, serious infections can cause Acanthamoeba keratitis, granulomatous amoebic encephalitis, skin, and lung infections. With an increasing number of Acanthamoeba infections in recent years, the pathogenicity of Acanthamoeba is becoming more relevant to mainstream clinical care. This review article will describe the etiological characteristics of Acanthamoeba infection in detail from the aspects of biological characteristic, classification, disease, and pathogenic mechanism in order to provide scientific basis for the diagnosis, treatment, and prevention of Acanthamoeba infection. more...
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- 2023
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10. Simulated precipitation change drives plant diversity and biomass change in the desert steppe
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Yuehua Wang, Zhongwu Wang, Haigang Li, Hailian Sun, Tingting Shen, Xiaohui Song, Zhanwen Wang, and Guodong Han
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Ecology ,Plant Science ,Ecology, Evolution, Behavior and Systematics - Abstract
There is a large uncertainty regarding the direction and magnitude of projected changes in the pattern and quantity of precipitation in future climate change scenarios. As changes in precipitation are likely to greatly impact plant diversity and biomass in desert ecosystems, we undertook a water addition/exclusion experiment to quantify the responses of plant diversity and biomass. To quantify the short-term impacts of changing the quantity of precipitation on plant species diversity and productivity in a desert steppe ecosystem. A simulated precipitation manipulation (addition and exclusion) experiment was made with four treatments: precipitation decreased by 50% (P-50%), precipitation increased by 50% (P+50%), precipitation increased by 100% (P+100%) and natural precipitation (Control). The richness of annual and biennial species was related to treatment; above-ground biomass increased in dry years in the P+100% treatment. Water addition had no statistically significant impacts on below-ground biomass. Increase in above-ground biomass with added precipitation in a drought year was mainly contributed by perennial grasses, suggesting that, from a management point of view, perennial grasses should be the focus of mitigation of projected future precipitation changes. more...
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- 2022
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11. CircATIC Contributes to Multiple Myeloma Progression via miR-324-5p-Dependent Regulation of HGF
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Bin Wu, Fang Wang, Yuehua Wang, Xianchao Deng, and Wangwei Wu
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MicroRNAs ,Hepatocyte Growth Factor ,Cell Movement ,Cell Line, Tumor ,Genetics ,Humans ,RNA, Circular ,General Medicine ,Multiple Myeloma ,Molecular Biology ,Biochemistry ,Ecology, Evolution, Behavior and Systematics ,Cell Proliferation - Abstract
Circular RNA (circRNA) 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (circATIC; hsa_circ_0058058) was observed to be upregulated in multiple myeloma (MM) by former article. However, the function and exact mechanism of circATIC in MM development remain barely known. CircRNA-microRNA (miRNA)-messenger RNA (mRNA) axis was established through using bioinformatic databases (starbase, Circinteractome, and microT-CDS). Dual-luciferase reporter assay, RNA immunoprecipitation assay, and RNA-pull down assay were utilized to verify the target relationship between microRNA-324-5p (miR-324-5p) and circATIC or hepatocyte growth factor (HGF). CircATIC expression was upregulated in MM patients and cell lines. CircATIC interference notably hampered cell proliferation, migration, invasion, and glycolysis and induced cell apoptosis of MM cells. MiR-324-5p was a target of circATIC. CircATIC silencing-mediated effects in MM cells were largely overturned by the knockdown of miR-324-5p. HGF was a target of miR-324-5p, and circATIC upregulated the expression of HGF partly through sponging miR-324-5p in MM cells. MiR-324-5p suppressed the malignant behaviors of MM cells, which were largely counteracted by the overexpression of HGF in MM cells. CircATIC accelerated the proliferation, migration, invasion, and glycolysis and suppressed the apoptosis of MM cells through mediating miR-324-5p/HGF signaling. more...
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- 2022
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12. Lonicera caerulea(Haskapberries): a review of development traceability, functional value, product development status, future opportunities, and challenges
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Zhen Cheng, Yiwen Bao, Zhiying Li, Jiaxin Wang, Mingshuang Wang, Sihang Wang, Yuanyuan Wang, Yuehua Wang, and Bin Li
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General Medicine ,Industrial and Manufacturing Engineering ,Food Science - Published
- 2022
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13. Diversity and function of microbial communities in the sand sheath of Agropyron cristatum by metagenomic analysis
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Meixiao Wu, Xuefei Wang, Hui Tang, Yijing Wang, Ming Yu, Yuehua Wang, and Guixia Liu
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Oscillatoria ,Microorganism ,Immunology ,Bulk soil ,General Medicine ,Biology ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Microbiology ,Agropyron cristatum ,Metagenomics ,Botany ,Genetics ,Phosphorus cycle ,Molecular Biology ,Nitrogen cycle ,Illumina dye sequencing - Abstract
The roots of most gramineous plants are surrounded by a variety of microorganisms; however, few studies have focused on the rhizosheath of psammophytes. Therefore, in this study, we used Illumina HiSeq high-throughput sequencing technology to analyse the composition and functional diversity of microbial communities in the rhizosheath of sand-grown Agropyron cristatum (L.) Gaertn. We found that the number of species and functions of microbial communities gradually decreased from the rhizosheath to the bulk soil. Thus, the microbial composition of the rhizosheath was richer and more diverse, and the abundance of bacteria, including Sphingosinicella, Rhizorhabdus, Friedmanniella, Geodermatophilus, Blastococcus, and Oscillatoria, was higher, and the abundance of fungi, such as Mycothermus, was higher. The abundance of CO2 fixation-related genes (acsA, Pcc, and cbbL) in the carbon cycle; NO3–, NO2–, NH2OH, and N2 transformation genes (nrtP, nirS, hao, and nifK) in the nitrogen cycle; soxB/A/C, Sat, and dsrB genes in the sulphur cycle; and 1-phosphate mannitol dehydrogenase (MtlD) gene and polyketide synthase gene (pks) were higher in the rhizosheath than in the bulk soil, as well as genes related to phosphorus uptake in the phosphorus cycle. Our findings showed that the rhizosheath may host the predominant microbial species related to the formation of a rhizosheath. more...
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- 2022
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14. Current knowledge of anthocyanin metabolism in the digestive tract: absorption, distribution, degradation, and interconversion
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Hailong Gui, Lijun Sun, Ruihai Liu, Xu Si, Dongnan Li, Yuehua Wang, Chi Shu, Xiyun Sun, Qiao Jiang, Yanyan Qiao, Bin Li, and Jinlong Tian
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General Medicine ,Industrial and Manufacturing Engineering ,Food Science - Abstract
Potential roles for anthocyanins in preventing various chronic diseases have been reported. These compounds are highly sensitive to external conditions and are susceptible to degradation, which increases the complexity of their metabolism in vivo. This review discusses anthocyanin metabolism in the digestive tract, phase I and II metabolism, and enterohepatic circulation (EHC), as well as their distribution of anthocyanins in blood, urine, and several organs. In the oral cavity, anthocyanins are partly hydrolyzed by microbiota into aglycones which are then conjugated by glucuronidase. In stomach, anthocyanins are absorbed without deglycosylation via specific transporters, such as sodium-dependent glucose co-transporter 1 and facilitative glucose transporters 1, while in small intestine, they are mainly absorbed as aglycones. High polymeric anthocyanins are easily degraded into low-polymeric forms or smaller phenolic acids by colonic microbiota, which improves their absorption. Anthocyanins and their derivatives are modified by phase I and II metabolic enzymes in cells and are released into the blood via the gastrovascular cavity into EHC. Notably, interconversion can be occurred under the action of enzymes such as catechol-O-methyltransferase. Taking together, differences in anthocyanin absorption, distribution, metabolism, and excretion largely depend on their glycoside and aglycone structures. more...
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- 2022
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15. Screening potential lncRNA biomarkers for breast cancer and colorectal cancer combining random walk and logistic matrix factorization
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Shijun Li, Miaomiao Chang, Ling Tong, Yuehua Wang, Meng Wang, and Fang Wang
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Genetics ,Molecular Medicine ,Genetics (clinical) - Abstract
Breast cancer and colorectal cancer are two of the most common malignant tumors worldwide. They cause the leading causes of cancer mortality. Many researches have demonstrated that long noncoding RNAs (lncRNAs) have close linkages with the occurrence and development of the two cancers. Therefore, it is essential to design an effective way to identify potential lncRNA biomarkers for them. In this study, we developed a computational method (LDA-RWLMF) by integrating random walk with restart and Logistic Matrix Factorization to investigate the roles of lncRNA biomarkers in the prognosis and diagnosis of the two cancers. We first fuse disease semantic and Gaussian association profile similarities and lncRNA functional and Gaussian association profile similarities. Second, we design a negative selection algorithm to extract negative LncRNA-Disease Associations (LDA) based on random walk. Third, we develop a logistic matrix factorization model to predict possible LDAs. We compare our proposed LDA-RWLMF method with four classical LDA prediction methods, that is, LNCSIM1, LNCSIM2, ILNCSIM, and IDSSIM. The results from 5-fold cross validation on the MNDR dataset show that LDA-RWLMF computes the best AUC value of 0.9312, outperforming the above four LDA prediction methods. Finally, we rank all lncRNA biomarkers for the two cancers after determining the performance of LDA-RWLMF, respectively. We find that 48 and 50 lncRNAs have the highest association scores with breast cancer and colorectal cancer among all lncRNAs known to associate with them on the MNDR dataset, respectively. We predict that lncRNAs HULC and HAR1A could be separately potential biomarkers for breast cancer and colorectal cancer and need to biomedical experimental validation. more...
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- 2023
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16. Multi-Sensor Eye-Tracking Systems and Tools for Capturing Student Attention and Understanding Engagement in Learning: A Review
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Shulan Lu, Yuehua Wang, and Derek Harter
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Computer science ,business.industry ,Data science ,Software ,Software deployment ,Component (UML) ,Eye tracking ,Virtual learning environment ,Systems design ,Use case ,Electrical and Electronic Engineering ,business ,Instrumentation ,Pace - Abstract
Dramatic advances in the design and deployment of sensors, edge communication and computing in recent years have enabled a significant shift and evolution in education. Pervasive learning in various learning environments, including face-to-face classrooms, online learning, virtual learning, and hybrid learning, is becoming our dominant learning paradigm for students who expect highly flexible and efficient options for studying at their own time, place, and pace. Especially, in our post-pandemic present, online learning will continue to gain in importance as a significant available and required learning component. This article provides a comprehensive review of the state-of-the-art systems and studies for assisting student learning and attempting to capture student attention and engagement in many different ways. It focuses on sensors and hardware, ranging from commercial multi-sensor eye-tracking devices to open-source, low-cost systems. We also explore and present system infrastructure, data features, data processing techniques, tools and software, and key technologies that are useful for enhancing many student learning environments. Additionally, the advantages, use cases, features, and limitations of different systems and techniques are explored and contrasted, where the results of our comparative analysis are summarized in tables for readers. This review article could assist both teachers and researchers to have a better understanding of current sensors, multi-sensor systems, and related technologies for capturing student attention and understanding their performance. It can also be applied to providing practical information for novel system design, promoting ongoing research studies, and fostering sensing and technology innovations for smart education. more...
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- 2021
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17. Establishment and psychometric characteristics of emotional words list for suicidal risk assessment in speech emotion recognition
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Juan, Shen, Shuo, Zhang, Yongsheng, Tong, Xiangmin, Dong, Xuelian, Wang, Guanghui, Fu, Liting, Zhao, Mengjie, Wu, Yi, Yin, Yuehua, Wang, Nancy H, Liu, Jianlan, Wu, and Jianqiang, Li
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Psychiatry and Mental health - Abstract
BackgroundEmotional disturbance is an important risk factor of suicidal behaviors. To ensure speech emotion recognition (SER), a novel technique to evaluate emotional characteristics of speech, precision in labeling emotional words is a prerequisite. Currently, a list of suicide-related emotional word is absent. The aims of this study were to establish an Emotional Words List for Suicidal Risk Assessment (EWLSRA) and test the reliability and validity of the list in a suicide-related SER task.MethodsSuicide-related emotion words were nominated and discussed by 10 suicide prevention professionals. Sixty-five tape-recordings of calls to a large psychological support hotline in China were selected to test psychometric characteristics of the EWLSRA.ResultsThe results shows that the EWLSRA consists of 11 emotion words which were highly associated with suicide risk scores and suicide attempts. Results of exploratory factor analysis support one-factor model of this list. The Fleiss’ Kappa value of 0.42 indicated good inter-rater reliability of the list. In terms of criteria validities, indices of despair (Spearman ρ = 0.54, P < 0.001), sadness (ρ = 0.37, P = 0.006), helplessness (ρ = 0.45, P = 0.001), and numbness (ρ = 0.35, P = 0.009) were significantly associated with suicidal risk scores. The index of the emotional word of numbness in callers with suicide attempt during the 12-month follow-up was significantly higher than that in callers without suicide attempt during the follow-up (P = 0.049).ConclusionThis study demonstrated that the EWLSRA has adequate psychometric performance in identifying suicide-related emotional words of recording of hotline callers to a national wide suicide prevention line. This list can be useful for SER in future studies on suicide prevention. more...
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- 2022
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18. Inhibition of Netosis with PAD Inhibitor Attenuates Endotoxin Shock Induced Systemic Inflammation
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Huanling Yao, Guojie Cao, Zheng Liu, Yue Zhao, Zhanchao Yan, Senzhen Wang, Yuehua Wang, Zhengwei Guo, and Yanming Wang
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Inflammation ,Lipopolysaccharides ,Neutrophils ,Organic Chemistry ,General Medicine ,Extracellular Traps ,Shock, Septic ,Catalysis ,Computer Science Applications ,Inorganic Chemistry ,Mice ,PAD inhibitor ,endotoxin shock ,netosis ,systemic inflammation ,LPS ,RNA-seq ,Protein-Arginine Deiminases ,Animals ,Cytokines ,Physical and Theoretical Chemistry ,Molecular Biology ,Spectroscopy - Abstract
Neutrophils play a pivotal role in innate immunity by releasing neutrophils extracellular traps (NETs). Excessive NETs are detrimental to the local tissue and further exacerbate inflammation. Protein arginine deiminases (PAD) mediate histone citrullination and NET formation that, in turn, exacerbate endotoxin shock damages. In this study, we further investigated the molecular mechanism underlying PAD and NETs in endotoxic stress in mice. The control group mice were injected with solvent, the LPS endotoxic shock group mice were intraperitoneally injected with LPS at 35 mg/kg only, while the LPS and PAD inhibitor YW3-56 treatment group mice were injected with YW3-56 at 10 mg/kg prior to the LPS injection. YW3-56 significantly prolonged the survival time of the LPS-treated mice. NETs, cfDNA, and inflammatory factors were detected by ELISA in serum, paitoneal cavity, and lung at 24 h after LPS administration. Lung injuries were detected by immunostaining, and lung tissue transcriptomes were analyzed by RNA-seq at 24 h after LPS administration. We found that YW3-56 altered neutrophil tissue homeostasis, inhibited NET formation, and significantly decreased cytokines (IL-6, TNFα and IL-1β) levels, cytokines gene expression, and lung tissue injury. In summary, NET formation inhibition offers a new avenue to manage inflammatory damages under endotoxic stress. more...
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- 2022
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19. Self-adaptive nonoverlapping sequential pattern mining
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Yan Li, Philippe Fournier-Viger, Fang Yao, Xindong Wu, Youxi Wu, and Yuehua Wang
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Set (abstract data type) ,Task (computing) ,Sequence ,Tree (data structure) ,Artificial Intelligence ,Computer science ,Key (cryptography) ,Filter (signal processing) ,Trial and error ,Time complexity ,Algorithm - Abstract
Repetitive sequential pattern mining (SPM) with gap constraints is a data analysis task that consists of identifying patterns (subsequences) appearing many times in a discrete sequence of symbols or events. By using gap constraints, the user can filter many meaningless patterns, and focus on those that are the most interesting for his needs. However, it is difficult to set appropriate gap constraints without prior knowledge. Hence, users generally find suitable constraints by trial and error, which is time-consuming. Besides, current algorithms are inefficient as they repeatedly check whether the gap constraints are satisfied. To address these problems, this paper presents a complete algorithm called SNP-Miner that has two key phases: candidate pattern generation and support (number of occurrences or occurrence frequency) calculation. To reduce the number of candidate patterns, SNP-Miner employs a pattern join strategy. Moreover, to efficiently calculate the support, SNP-Miner uses an incomplete Nettree structure stored in an array, and scans the structure once to avoid redundant calculations and reduce the time complexity. Experimental results show that SNP-Miner not only outperforms competitive algorithms, but can also discover more valuable patterns without user-predefined gap constraints. Algorithms and data can be downloaded from https://github.com/wuc567/Pattern-Mining/tree/master/SNP-Miner . more...
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- 2021
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20. Cyanidin-3-O-glucoside and its phenolic metabolites ameliorate intestinal diseases via modulating intestinal mucosal immune system: potential mechanisms and therapeutic strategies
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Qiao Jiang, Bin Li, Xu Si, Zhiying Li, Yuehua Wang, Xianjun Meng, Jinlong Tian, Chi Shu, Hui Tan, Yi Chen, Zhihuan Zang, Zhen Cheng, and Xiyun Sun
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food and beverages ,General Medicine ,Disease ,Biology ,Gut flora ,Pharmacology ,Cyanidin 3-O-glucoside ,biology.organism_classification ,Industrial and Manufacturing Engineering ,In vitro ,Immune system ,Intestinal mucosa ,In vivo ,Gut homeostasis ,Food Science - Abstract
The incidence of the intestinal disease is globally increasing, and the intestinal mucosa immune system is an important defense line. A potential environmental cause to regulate gut health is diet. Cyanidin-3-O-glucoside is a natural plant bioactive substance that has shown rising evidence of improving intestinal disease and keeping gut homeostasis. This review summarized the intestinal protective effect of Cyanidin-3-O-glucoside in vivo and in vitro and discussed the potential mechanisms by regulating the intestinal mucosal immune system. Cyanidin-3-O-glucoside and phenolic metabolites inhibited the presence and progression of intestinal diseases and explained from the aspects of repairing the intestinal wall, inhibiting inflammatory reaction, and regulating the gut microbiota. Although the animal and clinical studies are inadequate, based on the accumulated evidence, we propose that the interaction of Cyanidin-3-O-glucoside with the intestinal mucosal immune system is at the core of most mechanisms by which affect host gut diseases. This review puts forward the potential mechanism of action and targeted treatment strategies. more...
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- 2021
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21. Suicide attempt risks among hotline callers with and without the coronavirus disease 2019 related psychological distress: a case-control study
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Cuiling Wang, Yongsheng Tong, Kenneth R. Conner, Yuehua Wang, Liting Zhao, Yi Yin, and Mengjie Wu
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medicine.medical_specialty ,China ,RC435-571 ,Prior suicide attempt history ,Context (language use) ,Suicide, Attempted ,Lower risk ,Psychological Distress ,Life events ,03 medical and health sciences ,0302 clinical medicine ,Moderator ,Hotlines ,medicine ,Suicide attempt ,Humans ,030212 general & internal medicine ,Psychiatry ,Coronavirus disease 2019 ,Hotline ,business.industry ,SARS-CoV-2 ,Research ,Case-control study ,COVID-19 ,Moderation ,Mental health ,030227 psychiatry ,Psychiatry and Mental health ,Distress ,Case-Control Studies ,business - Abstract
Background The coronavirus disease 2019 (COVID-19) pandemic profoundly impacts on mental health, yet it is still unclear whether COVID-19 distress makes people more vulnerable to suicidal behavior. The present study aims to examine the association between COVID-19 related psychological distress and risk for suicide attempt, and moderators of this association, among hotline callers. Methods This case-control study was conducted at the largest psychological support hotline in China. Hotline callers who sought help for psychological distress and reported whether or not they attempted suicide in the last 2 weeks (recent suicide attempt) were analyzed. The primary predictor of recent suicide attempt was the presence or absence of COVID-19 related psychological distress. Demographic variables and common risk and protective factors for suicidal behavior were also studied. Callers with COVID-19 related distress (COVID-19 callers) and those without such distress (non-COVID-19 callers) were compared on these variables. Recent suicide attempt was regressed on COVID-19 related distress and the other variables, and significant interaction terms of aforementioned predictors by COVID-19 related distress, to identify variables that moderate the association of COVID-19 related distress and recent suicide attempt. Results Among 7337 included callers, there were 1252 COVID-19 callers (17.1%) and 6085 non-COVID-19 callers (82.9%). The COVID-19 callers were less likely to report recent suicide attempt (n = 73, 5.8%) than the non-COVID-19 callers (n = 498, 8.2%, P = 0.005). The COVID-19 callers were also less likely to have high scores on depressive symptoms (22.6% vs 26.3%, P P P P = 0.021), and a trend that suicide attempt history was associated with two-thirds greater risk (P = 0.063) for recent suicide attempt, among COVID-19 callers than non-COVID-19 callers. Conclusions The COVID-19 calls are from individuals with lower suicide-related risk compared to more typical callers. Acute stressful life events provided a key context for suicide attempt in non-COVID-19 callers, i.e., more typical calls. more...
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- 2021
22. Analysis on construction index and standard of water-saving units of public institutions in southern water-rich region
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Haibo Xu and Yuehua Wang
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- 2022
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23. TcJAV3-TcWRKY26 Cascade Is a Missing Link in the Jasmonate-Activated Expression of Taxol Biosynthesis Gene
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Li, Chen, Ling, Wu, Liu, Yang, Haiyang, Yu, Pingliang, Huang, Yuehua, Wang, Ruifeng, Yao, and Meng, Zhang
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Paclitaxel ,Gene Expression Regulation, Plant ,Arabidopsis Proteins ,Oxylipins ,Cyclopentanes ,Taxus - Abstract
Jasmonates (JAs) are the most effective inducers for the biosynthesis of various secondary metabolites. Currently, jasmonate ZIM domain (JAZ) and its interactors, such as MYC2, constitute the main JA signal transduction cascade, and such a cascade fails to directly regulate all the taxol biosynthesis genes, especially the rate-limit gene more...
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- 2022
24. Strigolactone signaling complex formation in yeast: A paradigm for studying hormone-induced receptor interaction with multiple downstream proteins
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Haiyang, Yu, Liu, Yang, Hongfan, Long, Xin, Su, Yuehua, Wang, Qiong, Xing, Ruifeng, Yao, Meng, Zhang, and Li, Chen
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Lactones ,Arabidopsis Proteins ,Gene Expression Regulation, Plant ,Saccharomyces cerevisiae ,Carrier Proteins ,Heterocyclic Compounds, 3-Ring ,Hormones ,Plant Proteins ,Signal Transduction - Abstract
Strigolactones (SLs) are bioactive carotenoid derivatives which function as signaling molecules to regulate plant architecture, nutrient absorption and communication with other organisms. The α/β-fold hydrolase, D14, hydrolyzes SLs, and the hydrolysis product activates D14 to bind to downstream signaling partners, including an E3 ubiquitin ligase MAX2 and SMXL6/7/8 proteins. What was not known was whether binding with one downstream partner would alter the affinity of D14 for other binding partners. Here, we developed an efficient yeast four-hybrid (Y4H) detection system and demonstrate that SL induces the interaction of D14 with both SMXL7 and MAX2 in a dose-dependent manner. Moreover, using our newly established yeast four-hybrid system, we found that the SL-induced D14 interaction with SMXL7 was strengthened by MAX2 while SMXL7 weakened the SL-induced D14 interaction with MAX2. Our findings provide novel insights into the regulatory effects of these signaling components and shed light on the molecular mechanism controlling the core SL signaling pathway. Furthermore, the heterologous yeast platform used for investigating SL complex formation has great potential to explore dynamic interactions in other signaling pathways or elucidate the unknown complex formation for biosynthesis of the parent carotenoids of SLs. more...
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- 2022
25. IL-24 inhibits the malignancy of human glioblastoma cells via destabilization of Zeb1
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Yuming Zhao, Yuehua Wang, Tie Lin, Zhan Zhang, Jun Chen, Dongpeng Wang, and Zhong Wu
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0301 basic medicine ,Clinical Biochemistry ,Malignancy ,Biochemistry ,Central Nervous System Neoplasms ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,Epithelial–mesenchymal transition ,Molecular Biology ,Transcription factor ,Cells, Cultured ,Messenger RNA ,Temozolomide ,Chemistry ,Interleukins ,Zinc Finger E-box-Binding Homeobox 1 ,Interleukin ,medicine.disease ,In vitro ,030104 developmental biology ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research ,Glioblastoma ,medicine.drug - Abstract
Glioblastoma (GBM) is the most common and fatal type of primary malignant tumours in the central nervous system. Cytokines such as interleukins (ILs) play an important role in GBM progression. Our present study found that IL-24 is down-regulated in GBM cells. Recombinant IL-24 (rIL-24) can suppress the in vitro migration and invasion of GBM cells while increase its chemo-sensitivity to temozolomide (TMZ) treatment. rIL-24 negatively regulates the expression of Zeb1, one well known transcription factors of epithelial to mesenchymal transition (EMT) of cancer cells. Over expression of Zeb1 can attenuate IL-24-suppressed malignancy of GBM cells. Mechanistically, IL-24 decreases the protein stability of Zeb1 while has no effect on its mRNA stability. It is due to that IL-24 can increase the expression of FBXO45, which can destabilize Zeb1 in cancer cells. Collectively, we reveal that IL-24 can suppress the malignancy of GBM cells via decreasing the expression of Zeb1. It suggests that targeted activation of IL-24 signals might be a potential therapy approach for GBM treatment. more...
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- 2021
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26. Fe and N co-doped graphene coated nanodiamond/Fe3C as highly active and stable catalyst for oxygen reduction reaction
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YueHua Wang, Jing Su, Guohao Zhu, Liang Dong, and Jianbing Zang
- Subjects
Mechanical Engineering ,Materials Chemistry ,General Chemistry ,Electrical and Electronic Engineering ,Electronic, Optical and Magnetic Materials - Published
- 2023
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27. Water causes divergent responses of specific carbon sink to long-term grazing in a desert grassland
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Yuxi Jin, Dashuan Tian, Jiangwen Li, Qian Wu, Zhanlei Pan, Mengqi Han, Yuehua Wang, Jun Zhang, and Guodong Han
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Environmental Engineering ,Environmental Chemistry ,Pollution ,Waste Management and Disposal - Published
- 2023
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28. Novel Peptide-Based Magnetic Nanoparticle for Mesenchymal Circulating Tumor Cells Detection
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Weikai Zhang, Zhiyuan Hu, Jierong Dong, Zihua Wang, Fanghao Shi, Zhiguo Fang, Yuehua Wang, and Fei Jia
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chemistry.chemical_classification ,Epithelial-Mesenchymal Transition ,Chemistry ,010401 analytical chemistry ,Mesenchymal stem cell ,Epithelial cell adhesion molecule ,Peptide ,Epithelial Cell Adhesion Molecule ,Neoplastic Cells, Circulating ,010402 general chemistry ,01 natural sciences ,0104 chemical sciences ,Analytical Chemistry ,Molecular analysis ,chemistry.chemical_compound ,Circulating tumor cell ,Cell culture ,Cell Line, Tumor ,Cancer cell ,Biomarkers, Tumor ,Cancer research ,Humans ,Epithelial–mesenchymal transition ,Magnetite Nanoparticles ,Peptides - Abstract
The monitoring of circulating tumor cells (CTCs) has recently served as a promising approach for assessing prognosis and evaluating cancer treatment. We have already developed a CTCs enrichment platform by EpCAM recognition peptide-functionalized magnetic nanoparticles (EP@MNPs). However, considering heterogeneous CTCs generated through epithelial-mesenchymal transition (EMT), mesenchymal CTCs would be missed with this method. Notably, N-cadherin, overexpressed on mesenchymal CTCs, can facilitate the migration of cancer cells. Hence, we screened a novel peptide targeting N-cadherin, NP, and developed a new CTCs isolation approach via NP@MNPs to complement EpCAM methods' deficiencies. NP@MNPs had a high capture efficiency (about 85%) of mesenchymal CTCs from spiked human blood. Subsequently, CTCs were captured and sequenced at the single-cell level via NP@MNPs and EP@MNPs, RNA profiles of which showed that epithelial and mesenchymal subgroups could be distinguished. Here, a novel CTCs isolation platform laid the foundation for mesenchymal CTCs isolation and subsequent molecular analysis. more...
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- 2021
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29. Gut Microbiota Modulation by Polyphenols from Aronia melanocarpa of LPS-Induced Liver Diseases in Rats
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Yuehua Wang, Chang Tan, Yanwen Kong, Tingcai Yan, Meizhi Wan, Haotian Deng, Xianjun Meng, Yuqi Tong, and Mingyue Wang
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0106 biological sciences ,biology ,medicine.diagnostic_test ,Lipopolysaccharide ,010401 analytical chemistry ,General Chemistry ,Pharmacology ,Gut flora ,biology.organism_classification ,medicine.disease ,01 natural sciences ,0104 chemical sciences ,chemistry.chemical_compound ,Liver disease ,Western blot ,chemistry ,biology.protein ,medicine ,Liver function ,Signal transduction ,General Agricultural and Biological Sciences ,Interleukin 6 ,Barrier function ,010606 plant biology & botany - Abstract
Aronia melanocarpa polyphenols (AMPs) can alleviate the degree of liver diseases in rats. However, the mechanism by which this is achieved through gut microbiota modulation remains unclear. Here, a rich-polyphenol extract of A. melanocarpa (AMPs) was used to treat lipopolysaccharide (LPS)-induced liver diseases in rats. To gain insights into the anti-LPS-induced liver disease, liver function index, expression of apoptosis proteins, inflammatory factors, and activation of inflammatory signaling pathways were determined with western blot analysis, immunohistochemistry, and 16S rRNA sequencing or quantitative real-time polymerase chain reaction (qRT-PCR). After AMPs treatment, the gut microbiota composition was modulated, promoting the intestinal barrier function by increasing the expression of intestinal epithelial cell tight junction proteins to reduce the LPS content in serum. The expression levels of inflammatory factors interleukin 6 (IL-6), interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), and related mRNAs were reduced. These results showed that AMPs, as a bioactive substance, could enhance the intestinal barrier function and modulate the gut microbiota of LPS-induced liver diseases. more...
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- 2021
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30. The mechanism and active compounds of semen armeniacae amarum treating coronavirus disease 2019 based on network pharmacology and molecular docking
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Zhenzhen Guo, Wenwen Gu, Yuji Niu, Yuehua Wang, Fuguang Kui, Wenwen Li, Yaru Zhang, Gangjun Du, and Fan Gao
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0301 basic medicine ,MAPK3 ,030209 endocrinology & metabolism ,target prediction ,lcsh:TX341-641 ,Computational biology ,Biology ,medicine.disease_cause ,03 medical and health sciences ,0302 clinical medicine ,semen armeniacae amarum ,medicine ,network pharmacology ,HRAS ,KEGG ,Coronavirus ,030109 nutrition & dietetics ,Nutrition and Dietetics ,Kinase ,Public Health, Environmental and Occupational Health ,molecular docking ,Vascular endothelial growth factor A ,covid-19 ,Original Article ,lcsh:Nutrition. Foods and food supply ,Food Science ,Systems pharmacology ,Proto-oncogene tyrosine-protein kinase Src - Abstract
Background: Coronavirus disease 2019 (COVID-19) outbreak is progressing rapidly, and poses significant threats to public health. A number of clinical practice results showed that traditional Chinese medicine (TCM) plays a significant role for COVID-19 treatment. Objective: To explore the active components and molecular mechanism of semen armeniacae amarum treating COVID-19 by network pharmacology and molecular docking technology. Methods: The active components and potential targets of semen armeniacae amarum were retrieved from traditional Chinese medicine systems pharmacology (TCMSP) database. Coronavirus disease 2019-associated targets were collected in the GeneCards, TTD, OMIM and PubChem database. Compound target, compound-target pathway and medicine-ingredient-target disease networks were constructed by Cytoscape 3.8.0. Protein-protein interaction (PPI) networks were drawn using the STRING database and Cytoscape 3.8.0 software. David database was used for gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. The main active components were verified by AutoDock Vina 1.1.2 software. A lipopolysaccharide (LPS)-induced lung inflammation model in Institute of Cancer Research (ICR) mice was constructed and treated with amygdalin to confirm effects of amygdalin on lung inflammation and its underlying mechanisms by western blot analyses and immunofluorescence. Results: The network analysis revealed that nine key, active components regulated eight targets (Proto-oncogene tyrosine-protein kinase SRC (SRC), interleukin 6 (IL6), mitogen-activated protein kinase 1 (MAPK1), mitogen-activated protein kinase 3 (MAPK3), vascular endothelial growth factor A (VEGFA), epidermal growth factor receptor (EGFR), HRAS proto-oncogene (HRAS), caspase-3 (CASP3)). Gene ontology and KEGG enrichment analysis suggested that semen armeniacae amarum plays a role in COVID-19 by modulating 94 biological processes, 13 molecular functions, 15 cellular components and 80 potential pathways. Molecular docking indicated that amygdalin had better binding activity to key targets such as IL6, SRC, MAPK3, SARS coronavirus-2 3C-like protease (SARS-CoV-2 3CLpro) and SARS-CoV-2 angiotensin converting enzyme II (ACE2). Experimental validation revealed that the lung pathological injury and inflammatory injury were significantly increased in the model group and were improved in the amygdalin group. Conclusion: Amygdalin is a candidate compound for COVID-19 treatment by regulating IL6, SRC, MAPK1 EGFR and VEGFA to involve in PI3K-Akt signalling pathway, VEGF signalling pathway and MAPK signalling pathway. Meanwhile, amygdalin has a strong affinity for SARS-CoV-2 3CLpro and SARS-CoV-2 ACE2 and therefore prevents the virus transcription and dissemination. more...
- Published
- 2021
31. Comparative study on anti-HCV testing using plasma, dried plasma spots (DPS), and dried blood spots (DBS)
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Wenge Xing, Yan Jiang, Lin He, Kai Chen, Jieqiong Ma, Yuehua Wang, and Yanan Ren
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Microbiology (medical) ,medicine.medical_specialty ,Gastroenterology ,lcsh:Infectious and parasitic diseases ,HCV Positive ,Serology ,Plasma ,Spearman's correlation coefficient ,Dried blood spots ,Internal medicine ,Medicine ,lcsh:RC109-216 ,Dried blood ,biology ,Spots ,business.industry ,Anti hiv ,lcsh:Public aspects of medicine ,Public Health, Environmental and Occupational Health ,lcsh:RA1-1270 ,Dried plasma spots ,Confidence interval ,Dried blood spot ,Infectious Diseases ,HCV ,biology.protein ,Antibody ,business ,Biotechnology - Abstract
The aim of this study was to evaluate the performance of an assay using dried plasma spot (DPS) and dried blood spot (DBS) samples for the serological detection of anti-hepatitis C virus (HCV) antibodies. Between January and July 2019, plasma, DPS and DBS specimens were collected from individuals at high-risk for HCV infection. Samples were tested for anti-HCV by ELISA, and the performance of DPS and DBS specimens was examined using results from the plasma testing, as the standard. Blood samples were collected from 329 persons, including 129 men who have sex with men and 200 intravenous drug users. Results from the plasma testing indicated that 118 samples (59.0%) were HCV positive. Data from the DPS sample testing showed sensitivity as 99.2% (95% confidence interval [CI]: 0.95–1.00) and specificity as 100% (95% CI: 0.98–1.00) for HCV detection, with Kappa of 99.3% (95% CI: 0.98–1.00) while in DBS sample testing the sensitivity as 98.3% (95% CI: 0.93–1.00) and specificity as 100% (95% CI: 0.98–1.00), with Kappa of 98.7% (95% CI: 0.97–1.00), respectively. Spearman’s correlation coefficients for the comparisons between plasma and DPS specimen, plasma and DBS specimens, DPS and DBS specimens were 0.857, 0.750, and 0.739, respectively. Compared with the results in plasma, 1 sample was not detected using the DPS specimens, and 2 samples were failed for the positive detection, using the DBS specimens. Both DPS and DBS samples were promising alternatives to plasma, for the detection of anti-HCV antibodies. more...
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- 2021
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32. A comparative investigation on physicochemical properties, chemical composition, and in vitro antioxidant activities of rice bran oils from different japonica rice (Oryza sativa L.) varieties
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Dong Zhang, Xiaoliang Duan, Yuanyuan Wang, Bo Shang, Hui Liu, Hui Sun, and Yuehua Wang
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General Chemical Engineering ,Safety, Risk, Reliability and Quality ,Industrial and Manufacturing Engineering ,Food Science - Published
- 2021
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33. Towards Collaborative and Intelligent Learning Environments Based on Eye Tracking Data and Learning Analytics: A Survey
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Yuehua Wang, Derek Harter, and Shulan Lu
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Schedule ,Proactive learning ,Artificial intelligence ,Data collection ,learning ,General Computer Science ,business.industry ,Computer science ,Distance education ,General Engineering ,Learning analytics ,Flexibility (personality) ,multimodal learning analytics ,Data science ,TK1-9971 ,Analytics ,attention measurement ,human–computer interaction ,Eye tracking ,General Materials Science ,data science ,Electrical engineering. Electronics. Nuclear engineering ,business - Abstract
The current pandemic has significantly impacted educational practices, modifying many aspects of how and when we learn. In particular, remote learning and the use of digital platforms have greatly increased in importance. Online teaching and e-learning provide many benefits for information retention and schedule flexibility in our on-demand world while breaking down barriers caused by geographic location, physical facilities, transportation issues, or physical impediments. However, educators and researchers have noticed that students face a learning and performance decline as a result of this sudden shift to online teaching and e-learning from classrooms around the world. In this paper, we focus on reviewing eye-tracking techniques and systems, data collection and management methods, datasets, and multi-modal learning data analytics for promoting pervasive and proactive learning in educational environments. We then describe and discuss the crucial challenges and open issues of current learning environments and data learning methods. The review and discussion show the potential of transforming traditional ways of teaching and learning in the classroom, and the feasibility of adaptively driving learning processes using eye-tracking, data science, multimodal learning analytics, and artificial intelligence. These findings call for further attention and research on collaborative and intelligent learning systems, plug-and-play devices and software modules, data science, and learning analytics methods for promoting the evolution of face-to-face learning and e-learning environments and enhancing student collaboration, engagement, and success. more...
- Published
- 2021
34. Simple Detection of DNA Methyltransferase with an Integrated Padlock Probe
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Yuehua Wang, Yingli Han, Fangyu Zhou, Tingting Fan, and Feng Liu
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Clinical Biochemistry ,Biomedical Engineering ,General Medicine ,Biosensing Techniques ,DNA ,Methyltransferases ,Analytical Chemistry ,Limit of Detection ,DNA methyltransferases ,padlock probe ,rolling circle amplification ,fluorescence ,Instrumentation ,Engineering (miscellaneous) ,Nucleic Acid Amplification Techniques ,Biotechnology ,DNA Primers - Abstract
DNA methyltransferases (MTases) can be regarded as biomarkers, as demonstrated by many studies on genetic diseases. Many researchers have developed biosensors to detect the activity of DNA MTases, and nucleic acid amplification, which need other probe assistance, is often used to improve the sensitivity of DNA MTases. However, there is no integrated probe that incorporates substrates and template and primer for detecting DNA MTases activity. Herein, we first designed a padlock probe (PP) to detect DNA MTases, which combines target detection with rolling circle amplification (RCA) without purification or other probe assistance. As the substrate of MTase, the PP was methylated and defended against HpaII, lambda exonuclease, and ExoI cleavage, as well as digestion, by adding MTase and the undestroyed PP started RCA. Thus, the fluorescent signal was capable of being rapidly detected after adding SYBRTM Gold to the RCA products. This method has a detection limit of approximately 0.0404 U/mL, and the linear range was 0.5–110 U/mL for M.SssI. Moreover, complex biological environment assays present prospects for possible application in intricacy environments. In addition, the designed detection system can also screen drugs or inhibitors for MTases. more...
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- 2022
35. Case Report: Disseminated Nocardiosis Caused by Nocardia vulneris in a Patient With Macroglobulinemia
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Fulan Qiu, Zhiyi Ma, Rongrong Zhong, Haonan Huang, Yuehua Wang, and Hui Liu
- Subjects
Public Health, Environmental and Occupational Health - Abstract
This report describes a case of disseminated nocardiosis, caused by Nocardia vulneris, in a 61-year-old man with macroglobulinemia and presenting with repeated fever, cough, shortness of breath, and muscle pain. The isolated Nocardia strain was resistant to ciprofloxacin, but susceptible to amikacin, gentamicin, tobramycin, linezolid, trimethoprim-sulfamethoxazole, amoxicillin/clavulanic, moxifloxacin, ceftriaxone, cefotaxim, and imipenem. The patient was started on combined meropenem and doxycycline treatment, followed by trimethoprim-sulfamethoxazole, which was subsequently switched to a combination treatment of linezolid, amikacin, and trimethoprim-sulfamethoxazole. The patient recovered, and his condition remained stable. Although infection by Nocardia vulneris is rare, and it is easy to miss detection in clinical practice, clinicians should be aware of the possibility of this infection. In addition, the MIC value of the drug sensitivity test should be ascertained when there is a wide choice of medicines. The current case was treated successfully with linezolid, amikacin, and trimethoprim-sulfamethoxazole. In cases of disseminated nocardiosis, the patient should be treated with antimicrobial therapy for at least 12 months. Furthermore, bacteriological examination and antimicrobial susceptibility testing should be performed regularly. more...
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- 2022
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36. Protective effects of blueberry anthocyanin extracts on hippocampal neuron damage induced by extremely low-frequency electromagnetic field
- Author
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Zihan Xu, Xiyun Sun, Yuehua Wang, and Liu Ning
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animal structures ,030309 nutrition & dietetics ,lcsh:TX341-641 ,Pharmacology ,medicine.disease_cause ,Nitric oxide ,Superoxide dismutase ,03 medical and health sciences ,chemistry.chemical_compound ,symbols.namesake ,0404 agricultural biotechnology ,Blueberry anthocyanin extracts ,Hippocampal neuron ,medicine ,Membrane potential ,0303 health sciences ,biology ,food and beverages ,04 agricultural and veterinary sciences ,Glutathione ,respiratory system ,Malondialdehyde ,040401 food science ,Extremely low-frequency electromagnetic field ,chemistry ,Anthocyanin ,biology.protein ,Nissl body ,symbols ,lcsh:Nutrition. Foods and food supply ,Oxidative stress ,Protective effects ,Food Science - Abstract
The protective effects of blueberry anthocyanin extracts against damage induced by extremely low-frequency electromagnetic field (ELF-EMF) were investigated in a rat model. Wistar rats were exposed to ELF-EMF with or without the administration of blueberry anthocyanin extracts (50, 100, and 200 mg/kg per day intragastrically once a day) for 30 days. Blueberry anthocyanin extracts supplementation inhibited the decrease in Nissl substance levels, cell membrane integrity, and mitochondrial membrane potential induced by ELF-EMF; prevented the increase in nitric oxide, malondialdehyde, and Ca2+ concentrations; suppressed superoxide dismutase and glutathione depletion; and enhanced the cognitive ability of the rats exposed to ELF-EMF. The protective effects of blueberry anthocyanin extracts against hippocampal neuron injury caused by ELF-EMF were dose-dependent. These results demonstrated that blueberry anthocyanin extracts suppress hippocampal neuron injury caused by ELF-EMF by inhibiting cell membrane damage and oxidative stress pathways, and suggested that blueberry anthocyanin treatment potentially prevents hippocampal neuron injury. more...
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- 2020
37. Blueberry malvidin-3-galactoside modulated gut microbial dysbiosis and microbial TCA cycle KEGG pathway disrupted in a liver cancer model induced by HepG2 cells
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Yunen Liu, Jie Lin, Yuehua Wang, Bin Li, Rui Hai Liu, Xianjun Meng, Xiyun Sun, Wang Weisheng, Ningxuan Gao, and Zhen Cheng
- Subjects
030309 nutrition & dietetics ,lcsh:TX341-641 ,medicine.disease_cause ,Blueberry ,Microbiology ,Clostridia ,03 medical and health sciences ,0404 agricultural biotechnology ,medicine ,HCC ,0303 health sciences ,metagenomics ,biology ,Ruminococcus ,04 agricultural and veterinary sciences ,Metabolism ,biology.organism_classification ,040401 food science ,Citric acid cycle ,microbial TCA cycle KEGG pathway ,Muribaculum intestinale ,Metagenomics ,OGDH ,malvidin-3-galactoside ,lcsh:Nutrition. Foods and food supply ,Bacteria ,Food Science - Abstract
HCC (Hepatocellular Carcinoma) is a critical health issue worldwide. Our previous animal experiment has confirmed that blueberry malvidin-3-galactoside (M3G) can regulate the progression of HCC. In this study, feces samples from the same batch of mice were collected to explore the regulatory mechanism of M3G on intestinal microbiota and microbial TCA cycle metabolism KEGG pathway in HCC mice based on 16S rRNA sequencing and metagenomics. Our results showed that blueberry M3G increased the microbial diversity and regulated the structure of intestinal microbiota in mice, such as increasing the abundance of Clostridia (butyric acid-producing bacteria), Oscillospira and Ruminococcus, and reducing the abundance of pathogenic Erysipelotrichi. Compared with the group of liver cancer (LC) and 5-fluorouracil (5 F), blueberry M3G significantly regulated microbial TCA cycle KEGG pathway via improving the expression of key proteins (porA, DLAT, aceE, PC and OGDH). Additionally, we found which the abundance of Muribaculum intestinale (M.intestinale) increased by blueberry M3G may be an important factor affecting the microbial TCA cycle KEGG pathway via the pearson correlation (R) analysis of protein and microbiota. Taken together, these results demonstrate that the blueberry M3G has the potential to be an intestinal microbiota regulator and an adjuvant to HCC therapy. more...
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- 2020
38. Malvidin-3-galactoside from blueberry suppresses the growth and metastasis potential of hepatocellular carcinoma cell Huh-7 by regulating apoptosis and metastases pathways
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Wang Weisheng, Jinlong Tian, Yunen Liu, Jie Lin, Chi Shu, Yuehua Wang, Bin Li, Zhen Cheng, and Rui Hai Liu
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MAPK/ERK pathway ,Cell cycle checkpoint ,030309 nutrition & dietetics ,Hepatocellular carcinoma ,Apoptosis ,lcsh:TX341-641 ,MAPK signaling ,Metastasis ,03 medical and health sciences ,0404 agricultural biotechnology ,medicine ,PTEN ,Protein kinase B ,Malvidin-3-galactoside ,0303 health sciences ,biology ,Chemistry ,Cell growth ,04 agricultural and veterinary sciences ,medicine.disease ,040401 food science ,Cell culture ,biology.protein ,Cancer research ,lcsh:Nutrition. Foods and food supply ,Food Science - Abstract
Malvidin-3-galactoside (Mv-3-gal) is the major anthocyanin monomer in blueberry anthocyanins. The compound is well-characterized by its anti-oncogenesis function in multiple organs including liver. In the current study, the mechanism driving the anti-hepatocellular carcinoma (HCC) function of Mv-3-gal was further explored by focusing on apoptosis and metastases pathways. HCC cell line Huh-7 was firstly administrated with Mv-3-gal of different concentrations and the effect of Mv-3-gal on cell proliferation, apoptosis, colony formation ability, metastasis potential as well as the activity of Akt/PTEN and MAPK pathways was assessed. The results showed that Mv-3-gal inhibited the cell proliferation and colony formation ability, induced cell cycle arrest and apoptosis in a dose-dependent manner. Regarding the metastasis potential, Mv-3-gal suppressed the migration and invasion potential of Huh-7 cells by regulating MMPs expression. Taken together, the findings highlighted the anti-HCC potent of Mv-3-gal, which was associated with the inhibition of Akt/PTEN, MAPK and MMP pathways. more...
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- 2020
39. The ability of miRNAs to induce mesenchymal-to-epithelial transition (MET) in cancer cells is highly dependent upon genetic background
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Amber Akbar, Lilya V. Matyunina, Mengnan Zhang, John F. McDonald, and Yuehua Wang
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Male ,0301 basic medicine ,Cancer Research ,Epithelial-Mesenchymal Transition ,Biology ,03 medical and health sciences ,0302 clinical medicine ,microRNA ,Gene expression ,Biomarkers, Tumor ,medicine ,Humans ,Gene ,Ovarian Neoplasms ,Binding Sites ,Transition (genetics) ,Mesenchymal stem cell ,Prostatic Neoplasms ,Cancer ,medicine.disease ,Cell biology ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,PC-3 Cells ,Cancer cell ,Female ,Cancer cell lines - Abstract
Understanding of the molecular basis of host cell-miRNA interactions is prerequisite to the successful application of miRNAs as potential therapeutic agents. We studied the morphological and molecular consequences of over expression of three sequence divergent miRNAs previously implicated in the mesenchymal-to-epithelial transition process (MET) in three distinct mesenchymal-like cancer cell lines. The ability of miRNAs to induce morphological changes characteristic of MET positively correlated with induced changes in the expression of genes previously implicated in the process. Variability in the responses of different mesenchymal-like cells to over expression of the same miRNAs was attributable to inherent differences in trans-regulatory profiles pre-disposing these cells to miRNA-induced MET. Collectively our results indicate that miRNA-mediated regulation of MET is a highly integrated process that is significantly modulated by the molecular background of individual cells. more...
- Published
- 2020
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40. Utility of urinary ctDNA to monitoring minimal residual disease in early breast cancer patients
- Author
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Fei Xie, Qiushi Sun, Yuehua Wang, Huajun Huang, Gege Guan, Huijie Liu, Jiayin Yan, and Ling Wang
- Subjects
Adult ,Oncology ,Cancer Research ,medicine.medical_specialty ,Neoplasm, Residual ,Concordance ,Urinary system ,Breast Neoplasms ,Urine ,Circulating Tumor DNA ,Breast cancer ,Internal medicine ,Biopsy ,Genetics ,medicine ,Humans ,0501 psychology and cognitive sciences ,Clinical significance ,Longitudinal Studies ,Neoplasm Staging ,0505 law ,medicine.diagnostic_test ,business.industry ,05 social sciences ,Hazard ratio ,General Medicine ,Middle Aged ,medicine.disease ,Minimal residual disease ,050501 criminology ,Female ,business ,050104 developmental & child psychology - Abstract
Background Cancer recurrence for patients with early breast cancer is significant. Patients will benefit from more non-invasive modes of monitoring and we aim to study the feasibility of urinary circulating tumor DNA (ctDNA) to monitor for residual disease (MRD). Methods In this longitudinal study, 300 early breast cancer patients were recruited prospectively. Measurements were taken prior to treatment and at different time points thereafter for a total of 8 measurements. Comparisons were made with healthy volunteers and patients without detectable mutations in urine specimens. Disease free relapse were correlated to both urinary DNA quantity and ctDNA concentration. Results Baseline index measurements showed 38% of patients with detectable mutations. The concordance with biopsy tissues was 97.3%. Overall, breast cancer patients had higher urinary DNA compared with healthy volunteers. Over time, fluctuations in urinary DNA was negligible in healthy volunteers, indicating the stability of the marker. Among the patients with detectable mutations, we observed that higher urinary DNA quantity measurements at 6-month and patients with positive mutations were associated with greater risk of relapse. Hazard ratios for patients in this category was 1.65 (95% CI 1.26-2.16) and 1.98 (95% CI 1.48-2.63) respectively. Conclusion Urinary DNA offers non-invasive probing and real-time monitoring of breast cancer relapse. Our results demonstrated clear clinical relevance in breast cancer and significant risk profiling of early breast cancer patients. This potentially aids to complement current cancer relapse monitoring and may help in early intervention. more...
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- 2020
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41. Beneficial effects of Aronia melanocarpa berry extract on hepatic insulin resistance in type 2 diabetes mellitus rats
- Author
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Xianjun Meng, Yuehua Wang, Chong Ning, Guang Xin, Jingjing Mu, and Bo Zhang
- Subjects
Male ,medicine.medical_specialty ,Carbohydrate metabolism ,Diet, High-Fat ,Diabetes Mellitus, Experimental ,Phosphatidylinositol 3-Kinases ,Insulin resistance ,Internal medicine ,Photinia ,medicine ,Animals ,Hypoglycemic Agents ,Glucose homeostasis ,Glycogen synthase ,biology ,Plant Extracts ,Glucokinase ,Chemistry ,Increased hepatic glycogen content ,medicine.disease ,Rats ,Insulin receptor ,Endocrinology ,Diabetes Mellitus, Type 2 ,Gene Expression Regulation ,Liver ,Fruit ,Insulin Receptor Substrate Proteins ,biology.protein ,Homeostatic model assessment ,Insulin Resistance ,Phosphatidylinositol 3-Kinase ,Proto-Oncogene Proteins c-akt ,Signal Transduction ,Food Science - Abstract
We aimed to investigate) the effects of Aronia melanocarpa berry extract (AMBE) on hepatic insulin resistance and its mechanism at the molecular level in high-fat diet (HFD)- and streptozotocin (STZ)-induced type 2 diabetes mellitus (T2DM) rats. The rats were supplemented with AMBE at doses of 100 and 400 mg/kg body weight (bw) daily for 8 weeks. AMBE significantly reduced blood glucose and serum insulin levels and the homeostatic model assessment for insulin resistance score; improved glucose tolerance; increased hepatic glycogen content; and regulated glucose metabolism enzyme activity, including glucokinase, pyruvate kinase, phosphoenolpyruvate carboxykinase, and glucose-6-phosphatase in the liver. AMBE also reduced lipid accumulation and oxidative stress along with inflammation in the hepatic tissue of T2DM rats and improved hepatic function. The phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway was activated by AMBE through the elevation of insulin receptor substrate-2, PI3K, Akt, and glycogen synthase kinase-3β phosphorylation and glucose transporter 2, which might contribute to the promotion of glycogen synthesis and improvement of hepatic insulin resistance. AMBE shows promise as an ingredient of functional foods for alleviating hepatic insulin resistance in T2DM. PRACTICAL APPLICATION: The extract from the berries of Aronia melanocarpa (Michx.) Elliott (AMBE), with its relatively high content of polyphenolic compounds, has been shown to exert hypoglycemic effects in animal models of diabetes. Our findings support the use of A. melanocarpa as a functional food additive for the alleviation of hepatic insulin resistance and the management of glucose homeostasis in T2DM. more...
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- 2020
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42. Prognostic Relevance of Estrogen Receptor Status in Circulating Tumor Cells in Breast Cancer Patients Treated With Endocrine Therapy
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Ying Zhou, Jinmei Zhou, Jinyi Xiao, Yuehua Wang, Hao Wang, Haoyuan Shi, Chunyan Yue, Fei Jia, Ping Li, Zhiyuan Hu, Yanlian Yang, Zefei Jiang, and Tao Wang
- Subjects
Cancer Research ,Oncology - Abstract
Recently, female breast cancer (BC) has surpassed lung cancer to occupy the first place of the most commonly diagnosed cancer. The unsatisfactory prognosis of endocrine therapy for breast cancer might be attributed to the discordance in estrogen receptor (ER) status between primary tumors and corresponding metastases, as well as temporal and spatial receptor status heterogeneity at point-in-time between biopsy and treatment. The purpose of this study was to evaluate the prognostic and predictive value of ER status in circulating tumor cells (CTCs) in BC patients. We analyzed ER expression on CTCs isolated using the Pep@MNPs method in 2.0 ml of blood samples from 70 patients with BC and 67 female controls. The predictive and prognostic value of ER expression in CTCs and immunohistochemistry results of biopsies for progression-free survival (PFS) and overall survival (OS) of patients in response to therapies were assessed. The detection rate for CTCs was 95.71% (67/70 patients), with a median of 8 CTCs within 2 ml of peripheral venous blood (PVB). A concordance of 76.56% in ER status between CTCs and corresponding primary tumor and 69.23% between CTCs and corresponding metastases was observed. We also found that patients with ER-positive CTCs (CTC ER+) had longer PFS and OS than those without ER-positive CTCs (CTC ER-). Our findings suggested that ER status in CTCs of BC patients may provide valuable predictive and prognostic insights into endocrine therapies, although further evaluation in larger prospective trials is required. more...
- Published
- 2022
43. Strigolactone signaling complex formation in yeast: A paradigm for studying hormone-induced receptor interaction with multiple downstream proteins
- Author
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Haiyang Yu, Liu Yang, Hongfan Long, Xin Su, Yuehua Wang, Qiong Xing, Ruifeng Yao, Meng Zhang, and Li Chen
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- 2022
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44. MADS-box protein MtSOC1c regulates flowering and seed development in Medicago truncatula
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Jianbo Yuan, Hongfan Long, Fang Qiu, Yuehua Wang, Meng Zhang, Yuehui Chao, and Li Chen
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Agronomy and Crop Science - Published
- 2023
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45. Dual Metal Site Fe Single Atom Catalyst with Improved Stability in Acidic Conditions
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Yuehua Wang, Shuang Li, Rui Xu, Junpeng Chen, Yifan Hao, Ke Li, Yan Li, Yingmei Li, and Jing Wang
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Physical and Theoretical Chemistry ,Catalysis ,General Environmental Science - Abstract
Dual atom catalysts (DACs) not only retain uniform active sites and high atomic utilization efficiency as the single atom catalysts, but the two adjacent metal sites also cooperate and play a synergistic role to achieve additional benefits. However, the relationships connecting their dual-site synergistic effects on catalytic performance are not well rationalized due to limited pairs available from experiments. Herein, Fe/M dual sites supported by nitrogen doped carbon (Fe/M-N-C whereby M from 3 d–5 d electron containing transition metals) have been screened as an oxygen reduction reaction (ORR) catalyst. The results show that the absorption strength of ORR intermediates on four nitrogen coordinated metals is weaker than the three coordinated metals, which promotes favourable ORR activities. As a result, we recommended FeIr, FeRh, FeRu and FeOs as promising ORR catalysts. Ab initio molecular dynamic (AIMD) simulations suggest Fe/M-N-C (M = Ir, Rh, Ru and Os) catalysts with encouraging structural stability at room temperature. Furthermore, it is found that the nitrogen atoms in-between metals are vulnerable sites for proton attacking, yet the protonation process demands high energy, even under O2 atmosphere, which underlines good tolerance under acidic conditions. This work provides a broad understanding of Fe based catalyst and a new direction for catalytic design. more...
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- 2023
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46. Neutrophil extracellular traps: A novel target for the treatment of stroke
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Ziyuan, Zhao, Zirong, Pan, Sen, Zhang, Guodong, Ma, Wen, Zhang, Junke, Song, Yuehua, Wang, Linglei, Kong, and Guanhua, Du
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Pharmacology ,Pharmacology (medical) - Abstract
Stroke is a threatening cerebrovascular disease caused by thrombus with high morbidity and mortality rates. Neutrophils are the first to be recruited in the brain after stroke, which aggravate brain injury through multiple mechanisms. Neutrophil extracellular traps (NETs), as a novel regulatory mechanism of neutrophils, can trap bacteria and secret antimicrobial molecules, thereby degrading pathogenic factors and killing bacteria. However, NETs also exacerbate certain non-infectious diseases by activating autoimmune or inflammatory responses. NETs have been found to play important roles in the pathological process of stroke in recent years. In this review, the mechanisms of NETs formation, the physiological roles of NETs, and the dynamic changes of NETs after stroke are summarized. NETs participate in stroke through various mechanisms. NETs promote the coagulation cascade and interact with platelets to induce thrombosis. tPA induces the degranulation of neutrophils to form NETs, leading to hemorrhagic transformation and thrombolytic resistance. NETs aggravate stroke by mediating inflammation, atherosclerosis and vascular injury. In addition, the regulation of NETs in stroke, the potential of NETs as biomarker and the treatment of stroke targeting NETs are discussed. The increasing evidences suggest that NETs may be a potential target for stroke treatment. Inhibition of NETs formation or promotion of NETs degradation plays protective effects in stroke. However, how to avoid the adverse effects of NETs-targeted therapy deserves further study. In summary, this review provides a reference for the pathogenesis, drug targets, biomarkers and drug development of NETs in stroke. more...
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- 2023
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47. A sub-freshness monitoring chitosan/starch-based colorimetric film for improving color recognition accuracy via controlling the pH value of the film-forming solution
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Bin Li, Yiwen Bao, Jiaxin Li, Jinfeng Bi, Qinqin Chen, Huijun Cui, Yuxuan Wang, Jinlong Tian, Chi Shu, Yuehua Wang, Yuxi Lang, Weijia Zhang, Hui Tan, Qunxing Huang, and Xu Si
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Anthocyanins ,Excipients ,Chitosan ,Food Packaging ,Colorimetry ,Starch ,General Medicine ,Hydrogen-Ion Concentration ,Food Science ,Analytical Chemistry - Abstract
The demand for intelligent packaging in food sub-freshness monitoring is increasing. Herein, a pH and NH
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- 2021
48. APACHE IV system helps to determine role of cholecystostomy in elderly patients with acute cholecystitis
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Zhimin Yao, Hua Jiang, Guo Guo, and Yuehua Wang
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medicine.medical_specialty ,Medicine (General) ,medicine.medical_treatment ,Cholecystitis, Acute ,APACHE IV ,Biochemistry ,R5-920 ,cholecystostomy ,Internal medicine ,medicine ,Chi-square test ,Acute cholecystitis ,Humans ,Cholecystectomy ,APACHE ,Aged ,Retrospective Studies ,Receiver operating characteristic ,business.industry ,Mortality rate ,Biochemistry (medical) ,Therapeutic effect ,Cell Biology ,General Medicine ,mortality ,elderly patient ,Treatment Outcome ,Health evaluation ,Cholecystostomy ,Acute Physiology and Chronic Health Evaluation ,business ,Retrospective Clinical Research Report - Abstract
Objective Cholecystostomy is a palliative treatment for patients unfit to undergo immediate cholecystectomy. Nevertheless, the role of cholecystostomy in the clinical management of such patients remains unclear. The Acute Physiology and Chronic Health Evaluation IV (APACHE IV) scoring system is useful for estimating the hospital mortality of high-risk patients. We evaluated the therapeutic effect of cholecystostomy by the APACHE IV scoring system in patients aged >65 years with acute cholecystitis. Methods In total, 597 patients aged >65 years with acute cholecystitis were retrospectively analyzed using APACHE IV scores. Results The fitness of the APACHE IV score prediction was good, with an area under the receiver operating characteristic curve of 0.894. The chi square independence test indicated that compared with conservative treatment, cholecystostomy may have different effects on mortality for patients whose estimated mortality rate was >10%. Comparison of the estimated mortality of patients before and after cholecystostomy indicated that the estimated mortality was significantly lower after than before puncture, both in the whole patient group and in the group with an estimated mortality of >10%. Conclusion The APACHE IV scoring system showed that cholecystostomy is a safe and effective treatment for elderly high-risk patients with acute cholecystitis. more...
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- 2021
49. Diversity and function of microbial communities in the sand sheath of
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Yuehua, Wang, Meixiao, Wu, Yijing, Wang, Xuefei, Wang, Ming, Yu, Guixia, Liu, and Hui, Tang
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Soil ,Sand ,Microbiota ,Metagenomics ,Agropyron ,Soil Microbiology - Abstract
The roots of most gramineous plants are surrounded by a variety of microorganisms; however, few studies have focused on the rhizosheath of psammophytes. Therefore, in this study, we used Illumina HiSeq high-throughput sequencing technology to analyse the composition and functional diversity of microbial communities in the rhizosheath of sand-grown more...
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- 2021
50. Identification of Conserved and Divergent Strigolactone Receptors in Sugarcane Reveals a Key Residue Crucial for Plant Branching Control
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Anqi Hu, Ruifeng Yao, Langtao Xiao, Kuiliang Feng, Qiaoqiao Zhao, Ling Wu, Yuehua Wang, Miao Xie, Meng Zhang, Zhenhua Ming, Jinping Zhao, Li Chen, and Xuemei Zhou
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MAX2 ,biology ,receptor ,Mutant ,Strigolactone ,Repressor ,Plant culture ,Plant Science ,biology.organism_classification ,Phenotype ,Cell biology ,SB1-1110 ,SMXL ,Arabidopsis ,sugarcane ,Arabidopsis thaliana ,D14 ,Signal transduction ,strigolactone ,Receptor ,Original Research - Abstract
Strigolactones (SLs) are a class of important plant hormones mainly regulating plant architecture such as branching, which is crucial for crop yield. It is valuable to study SL signaling pathway and its physiological function in sugarcane, the most important sugar crop, for further molecular breeding. Here, two putative SL receptors SsD14a/b and the interacting F-box protein SsMAX2 were identified in Saccharum spontaneum. SL induced both SsD14a and SsD14b to interact with SsMAX2 in yeast. SsD14a, but not SsD14b, could bind with AtMAX2 and AtSMXL7/SsSMXL7. Overexpression of SsD14a or SsMAX2 rescued the increased branching phenotypes of Arabidopsis thaliana d14-1 or max2-3 mutants, respectively. Moreover, the crystal structure of N-terminal truncated SsD14a was solved, with an overall structure identical to AtD14 and OsD14 in the open state, consistent with its conserved branching suppression capacity in Arabidopsis. In line with the biochemical observations, SsD14b could not completely complement in d14-1 although these two SsD14 proteins have almost identical primary sequences except for very few residues. Complement with the combination of SsD14b and SsMAX2 still failed to rescue the d14-1 max2-3 double mutant multi-branching phenotype, indicating SsD14b–AtSMXL7 complex formation is required for regulating branching. Mutagenesis analyses revealed that residue R310 at α10 helix of SsD14a was crucial for the binding with SsSMXL7/AtSMXL7 but not SsMAX2. The site-equivalent single-residue P304R substitution enabled SsD14b to bind with AtMAX2 and AtSMXL7/SsSMXL7 and to rescue the phenotype of d14-1 max2-3 together with SsMAX2. Moreover, this conserved Arg residue across species including rice and Arabidopsis determined the activity of SL receptors through maintaining their interaction with SMXL repressors. Taken together, our work identified conserved and divergent strigolactone receptors in sugarcane core SL signaling pathway and revealed a key residue crucial for plant branching control. more...
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- 2021
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