Search

Accurate blood glucose determination is essential to the clinical diagnosis and management of diabetes. This work establishes an inner filter effect (IFE) strategy between upconversion nanoparticles (UCNPs) and quinone-imine complex for glucose monitoring in human serum simply and efficiently. In this system, the enzyme glucose oxidase (GOx) catalyzes the reaction of glucose into hydrogen peroxide (H2O2) and gluconic acid when compulsion by oxygen. In the presence of horseradish peroxidase (HRP), the produced H2O2 can catalytically oxidize phenol and 4-amino antipyrine (4-AAP) to generate quinone-imine products. The purple-colored quinone-imine complex effectively absorbed the fluorescence of NaYF4:Yb3+, Er3+ UCNPs, leading to the strong fluorescence quenching of UCNPs through IFE. Thus, a new approach was established for glucose monitoring by determining the fluorescence intensity. Under the optimal condition, this approach shows better linearity to glucose from 2–240 μmol/L with a low detection limit at 1.0 μmol/L. Owing to the excellent fluorescence property and background-free interference of the UCNPs, the biosensor was applied for glucose measurements in human serum and got a satisfactory result. Furthermore, this sensitive and selective biosensor revealed great potential for the quantitative analysis of blood glucose or different kinds of H2O2-involved biomolecules for the application of clinical diagnosis.

Additional file 2: Table S3. The list of primer sequences involved in qPCR.

Additional file 1: Table S2. The list of antibody and dilution ratio.

Supplemental material, sj-docx-2-tct-10.1177_15330338211066200 for A Rapid Cytological Screening as pre-Endoscopy Screening for Early Esophageal Squamous Cell Lesions: A Prospective Pilot Study from a Chinese Academic Center by Yadong Feng, Yan Liang, Bin Yao, Jiajia Xu, Juncai Zang, Youyu Zhang, Jiong Zhang, Guangpeng Xu, Bo Wei, Xiangyi Yao, Peilin Huang and Ruihua Shi in Technology in Cancer Research & Treatment

Supplemental material, sj-docx-2-tct-10.1177_15330338211066200 for A Rapid Cytological Screening as pre-Endoscopy Screening for Early Esophageal Squamous Cell Lesions: A Prospective Pilot Study from a Chinese Academic Center by Yadong Feng, Yan Liang, Bin Yao, Jiajia Xu, Juncai Zang, Youyu Zhang, Jiong Zhang, Guangpeng Xu, Bo Wei, Xiangyi Yao, Peilin Huang and Ruihua Shi in Technology in Cancer Research & Treatment

Background Cervical spondylotic myelopathy (CSM) is the most severe type of cervical spondylosis and the most common cause of spinal cord dysfunction among adults over 55 years old. MRI plays an important role in the diagnosis and evaluation of CSM, which can directly demonstrate the correlation between disc, spinal cord, posterior structures and abnormal signal in spinal cord. Static MRI can only show the static and neutral position of spinal cord, which is not enough to understand the pathogenesis of CSM. Dynamic MRI demonstrating the extension and flexion position of spinal cord can be a better tool for the treatment of CSM, especially the surgical decision making. Method A total of 180 CSM patients who have indications for surgery will be recruited in outpatient of Peking University Third Hospital and assigned to three groups (Group A, B and C) based on their static MRI after consent. Group A (incomplete dura compression) means the signal of cerebral spinal fluid (CSF) is still visible. Group B (complete dura compression) means no CSF signal and no shape change of spinal cord. Group C (spinal cord compression) means shape change of spinal cord. Two surgical plans will be made for each participant by one professional surgeon according to the static MRI and dynamic MRI respectively and we will randomly choose one to perform via a random number system. Follow-up will be maintained at 3, 6, and 12 months after surgery through outpatient or telephone interview, including mJOA score, 10-s G&R (grip and release) and 10-s step test, SF-36 score, radiographic examination and complications. Finally, data collection and statistical analysis will be finished by researchers who are blinded to recruitment and treatment. Discussion This study will help us to explore the indication of dynamic MRI and the value of dynamic MRI in the treatment of CSM, especially the surgical decision making. Dynamic MRI can be a useful tool in the treatment of CSM patients. Trial registration ChiCTR1900023014. Registered on May 7th, 2019.

In the fields of oilfield exploitation and petrochemical industry, demulsification and dewatering is one of the key processes in the resource utilization of emulsion. The device embedded with high-voltage electric field can achieve efficient demulsification and dewatering treatment, but the cone structure of the device has a great influence on the flow field. Therefore, a new double-field coupling dewatering and purification device with a composite curve–cone structure is proposed through the design and optimization of the device's cone structure. The mixture model was used to simulate the multiphase flow, the computation fluid dynamics (CFD) and population balance model (PBM) were used for numerical calculation in Fluent, and the optimal structure is obtained by combining the Box–Behnken factor design and the response surface method. Furthermore, the flow field and separation characteristics of the new structure and traditional structure are compared. Results show that when the structural parameters a, b and Ro are 110, 4, and 9 mm, respectively, the new coupling device has a high separation efficiency, which is approximately 97.03%. The separation efficiency of the new structure is 6.68% higher than that of the traditional structure. The composite curve-cone coupling device is more beneficial to the oil-water separation.

Despite many studies concerning the forming age, evolution characteristics and the age of petroleum charging in the Fuxin upheaval of southern Songliao Basin, no consensus has been reached so far. This paper presents the first K-Ar dating of autogenetic illite from stratigraphic petroleum reservoirs in the Fuyu oil layer of the Fuxin upheaval belt. Isotopic test and age calculation were carried out based on the separation and purification of illite mineral, X-diffraction analysis and the detection of scanning electron microscopy. The evolution characteristics of structure, sedimentation, reservoir-forming about the Fuxin upheaval belt were interpreted in terms of the synthetical analysis of “six-type geological history” evolution in southern Songliao Basin. The geologic background of petroleum evolution and reservoir formation are similar in the entire central depression region of southern Songliao Basin. The Changling sag and the Fuxin upheaval belt brought about obvious upheaval-sag separation after the hydrocarbon-generation peak of K2qn1 and the main reservoir-forming period of the Fuyu oil layer, namely reservoir-forming happened before the Fuxin upheaval belt extensively raised. The reservoirs have three characteristics: the hydrocarbon source rock above the reservoir, the oil source in the locality, and the vertical migration. The geological cognition is corrected, that is, oil source came from the Changling sag and migrated from the side direction. The bulk process of petroleum charging in the stratigraphic hydrocarbon reservoirs in the Fuxin upheaval belt of southern Songlio Basin is determined according to the isotopic age of autogenetic illite in combination with the method of fluid inclusions. The cognition is helpful to exactly evaluate the resource potential and exploration direction in the Fuxin upheaval belt, Changling sag and their peripheral areas. The present results indicate that the combination of the two methods (the K-Ar dating of autogenetic illite and fluid inclusions) is an effective way to lay bare petroleum charging history and ascertain reservoir age.

A three-dimensional combined technique, in situ quartz crystal microbalance in combination with infrared spectroscopy and electrochemistry was proposed. With this combining technique, the depositing process of poly- o -aminophenol on gold electrode surface was monitored. The oxidation and polymerization of the o -aminophenol, as well as the electrochemical properties of poly- o -aminophenol in acidic solution were investigated. Information about mass coverage of polymer, the doping/dedoping process, and change in structure of the polymer were obtained simultaneously and discussed. The results indicated that this method could be widely applied to the investigation of electrode-surface-relevant processes.

The combination of reflectance UV-Vis spectroelectrochemistry with electrochemical quartz crystal microbalance (EQCM) and separate reflectance FTIR characterization were used to investigate the structural interconversion for poly(o-phenylenediamine) (PoPD) between its ladder structure with phenazine units and polyaniline-like linear chains. The poly(o-phenylenediamine) films potentiostatically (0.8 V vs. SCE) grew on Au electrodes from 0.20 mol.L-1 H2SO4 (PoPD1) or 0.40 mol.L-1 NaOH (PoPD2) aqueous solution containing 0.20 mol.L-1 Na2SO4 + 0.10 mol.L-1 o-phenylenediamine. By considering the mass of deposited PoPD2 film obtained from the EQCM data and the charge consumed under the current peak at ca. 0.6 V vs. SCE for oxidation of -NH2 groups in as-prepared PoPD2 during potential cycling in 0.10 mol.L-1 aqueous H2SO4, the molar percentage of the polyaniline-like chains was estimated to be 19% (relative to total phenylenediamine units), being in agreement with the result obtained from a formaldehydecombination experiment through the aminocarbonyl reaction. After 40-cycle potential sweeps between 0.2 and 0.8 V vs. SCE the polyaniline-like chains in PoPD2 could be completely converted via intramolecular cyclization into the ladder structure with phenazine units. However, PoPu1 was found to be perfectly composed of the ladder structure with phenazine units, and after 40-cycle potential sweeps between-0.4 and 0.1 V vs. SCE only 2.5% in molar percentage of PoPD’s ladder structure could be converted into polyani line-like chains, suggesting that the ladder structure with phenazine units is thermodynamically more stable due to its possessing higher conjugation.

Q. People both advocate and support the reform of the political system. However, we have not completely gotten rid of the doctrine that academic issues may be discussed while political issues may not be discussed. For this reason, some people still hesitate to express their ideas.

Zhang Youyu, vice-director of the Law Committee of the National People's Congress, reported to the seventh session of the Sixth National People's Congress Standing Committee on the result of discussions on the Forestry Law of the People's Republic of China (revised draft) on the afternoon of September 11.