1. LncRNA RHPN1-AS1 Targeting miR-625/REG3A Promotes Cell Proliferation And Invasion Of Glioma Cells
- Author
-
Cui,Peng, Su,Jichun, Li,Qingmin, Xu,Guangming, and Zhu,Ningxi
- Subjects
OncoTargets and Therapy - Abstract
Peng Cui,1,2 Jichun Su,1 Qingmin Li,1 Guangming Xu,3 Ningxi Zhu1 1Department of Neurosurgery, Taian Center Hospital, Taian 271000, People’s Republic of China; 2School of Medicine, Shandong University, Jinan 250000, People’s Republic of China; 3Department of Neurosurgery, Shandong Provincial Hospital, Jinan 250021, People’s Republic of ChinaCorrespondence: Guangming XuDepartment of Neurosurgery, Shandong Provincial Hospital, No.324 Jingwuweiqi Road, Jinan 250021, People’s Republic of ChinaTel +86 531 87938911Email azhe610@163.comIntroduction: Glioma arises from the proliferation of neuroglial cells differentiated from the ectoderm. Evidence has confirmed that differentially expressed long non-coding RNAs (lncRNAs) may be involved in the development and progression of various tumors. The present study aimed to explore the biological function of lncRNA RHPN1-AS1 in glioma.Materials and methods: The expressions of RHPN1-AS1 in glioma tissues and cells were examined using RT-PCR. Colony formation assay, MTT assay, wound healing assay and transwell assay were performed to detect cell cloning efficiency, proliferation, migration and invasion of glioma cells, respectively. Western blot was applied to assess the expression levels of migration-related and invasion-related proteins. Online bioinformatic tools and luciferase reporter assay were, respectively, employed to predict and verify the downstream target microRNA/gene of RHPN1-AS1.Results: RHPN1-AS1 was up-regulated in glioma tissues and cells. The cell proliferation, migration and invasion of glioma were inhibited when the expression of RHPN1-AS1 was down-regulated in glioma cells. The expressions of migration-related and invasion-related proteins were also suppressed in siRHPN1-AS1 groups. Furthermore, we predicted and verified that RHPN1-AS1 was directly targeted to miR-625-5p/REG3A. Our study demonstrated that the knockdown of RHPN1-AS1 inhibited the proliferation, migration and invasion activity of glioma cells via regulating miR-625-5p/REG3A expression.Conclusion: The results revealed that the lncRNA RHPN1-AS1 may be a molecular target in glioma therapy.Keywords: glioma, LncRNA, RHPN1-AS1, proliferation, migration, invasion
- Published
- 2019