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Remanufacturing used products is an important technological approach in sustainable development and circular economy. Meanwhile, redesign is the key component of remanufacturing, as it can innovate the function and structure of used products. However, due to the uncertain quality, variety, and small batches of the returned used products for remanufacturing, it is difficult to generate a sound redesign scheme to satisfy the customer demand quickly and dynamically. In addition, it is unpredictable whether the redesign scheme is suitable for the remanufacturing processes, which may lead to additional remanufacturing costs. In order to improve the efficiency of design and obtain the optimal design scheme, it is necessary to use intelligent technology to quickly generate and optimize the redesign scheme. To address this, an intelligent redesign method for used products based on the digital twin is proposed in this paper. Digital twin (DT) technology can connect the physical world with the virtual world and use the virtual model to simulate the redesign process, which is conducive to the dynamic adjustment and optimization of the redesign scheme. Firstly, the redesign process framework is constructed based on the axiomatic design (AD) method, and the redesign features of the used products are analyzed to determine the redesign problems. Then, based on the connotation of a digital twin, an intelligent redesign framework is constructed, which provides detailed guidance for building the digital-twin-driven redesign system. Henceforth, the application of the redesign process based on a digital twin is discussed, a technical approach of the digital-twin-driven redesign is proposed, and data processing methods, such as data cleaning, data integration, and data analysis, are used to realize the redesign scheme decision. Finally, the feasibility of this method is verified by the redesign of a used clutch.

Supplementary Fig. S4 FAT10 stabilizes eEF1A1 protein levels by attenuating eEF1A1 ubiquitination and degradation in cancer cells.

Supplementary Figures Legends

Supplementary Fig. S3 Identification of the Ub binding site in eEF1A1.

Supplementary Fig. S2 shRNA-resistant FAT10 plasmid rescues the expression of eEF1A1 and recovers cell proliferation in shFAT10 cancer cells.

Human HLA-F adjacent transcript 10 (FAT10) is the only ubiquitin-like protein that can directly target substrates for degradation by proteasomes, but it can also stabilize the expression of certain substrates by antagonizing ubiquitination, through mechanisms as yet uncharacterized. In this study, we show how FAT10 stabilizes the translation elongation factor eEF1A1, which contributes to cancer cell proliferation. FAT10 overexpression increased expression of eEF1A1, which was sufficient to promote proliferation of cancer cells. Mechanistic investigations revealed that FAT10 competed with ubiquitin (Ub) for binding to the same lysines on eEF1A1 to form either FAT10–eEF1A1 or Ub–eEF1A1 complexes, respectively, such that FAT10 overexpression decreased Ub–eEF1A1 levels and increased FAT10–eEF1A1 levels. Overall, our work establishes a novel mechanism through which FAT10 stabilizes its substrates, advancing understanding of the biological function of FAT10 and its role in cancer. Cancer Res; 76(16); 4897–907. ©2016 AACR.

Supplementary Fig. S1 FAT10, eEF1A1, Rpn10, Ub, expression decreased after transfection of the corresponding shRNA plasmids.

Primers and shRNA target sequences

Supplementary Material and Methods

Aims Although increased production of malondialdehyde (MDA), an end product of lipid oxidation caused by reactive oxygen species (ROS), has been found be elevated in hypertensive population, whether MDA contributed to a changed risk of hypertension is uncertain. We aimed to investigate whether elevated blood levels of MDA contribute to increased risk of hypertension and obesity has a modified effect on the association in an older Chinese population. Methods Data were obtained from 2011 to 2012 of the Chinese Longitudinal Healthy Longevity Survey (CLHLS), a national cohort of older adults in China. Associations between blood MDA level and systolic and diastolic blood pressure (BP) and risk of hypertension were performed by multivariable linear regression and logistic regression analysis. Results The results of smooth curve revealed a gradual upward trend on association of blood MDA level with diastolic BP (P P > 0.05). Logistic regression analysis suggested that elevated blood MDA levels were associated with increased risk of diastolic hypertension (OR = 1.079, 95% CI 1.039–1.122, P OR = 0.978, 95% CI 0.943–1.015, P = 0.247) after adjustments of related confounding factors were made. Furthermore, we found the significant modification effect of obesity on the association between MDA level and risk of diastolic hypertension evaluated by body mass index (BMI, interaction P = 0.015) and by waist circumference (interaction P = 0.016). Conclusion Our results firstly identified that increased blood MDA levels were associated with elevated risk of diastolic hypertension, rather than systolic hypertension in the non-obese old population.

This study aimed to analyze the genetics and treatments of the patients with the triad of diabetic ketoacidosis (DKA), hypertriglyceridemia, and acute pancreatitis (AP).We conducted a retrospective study of six patients with the triad of AP, hypertriglyceridemia, and DKA at our hospital. All patients underwent plasmapheresis as part of their treatment. The clinical characteristics of the patients were obtained from the hospital information system and analyzed. Whole exome sequencing was performed using samples of one patient (case 6) and his family members.The average triglyceride level before plasmapheresis was 3282.17 ± 2975.43 mg/dL (range: 1646-9332 mg/dL). The triglyceride levels dropped by approximately 80% after plasmapheresis. None of the patients developed complications related from plasmapheresis. During follow-up, patients 5 and 6 developed recurrent pancreatitis for several times and showed the formation of pancreatic pseudocysts. We identified three novel heterozygous missense mutations in the family of patient 6, including c.12614C T (p.Pro4205Leu) in APOB, c.160G C (p.Glu54Gln) in CILP2, and c.1199C A (p.Ala400Glu) in PEPD.Three novel heterozygous missense mutations, including c.12614C T (p.Pro4205Leu) in APOB, c.160G C (p.Glu54Gln) in CILP2, and c.1199C A (p.Ala400Glu) in PEPD were first identified in a patient with the triad of DKA, hypertriglyceridemia, and AP. The combination of plasmapheresis, hydration, and insulin therapy may have the greatest clinical benefits for these patients.背景: 本研究旨在分析糖尿病酮症酸中毒(DKA)、高三酰甘油血症和急性胰腺炎(AP)三联症患者的遗传学基础和治疗方法。 方法: 我们对我院的6例AP、高三酰甘油血症和DKA三联症患者进行回顾性研究。所有患者均接受血浆置换术作为治疗的一部分。从医院信息系统获得患者的临床特征并进行分析。使用一名患者(病例6)及其家庭成员的血样进行全外显子组测序。 结果: 血浆去除前的平均三酰甘油水平为3281.17±2975.43 mg/dL(范围:1646-9332 mg/dL)。血浆置换后的三酰甘油水平下降了约80%。没有患者出现血浆置换相关的并发症。在随访期间, 患者5和6反复发作几次胰腺炎, 并形成胰腺假性囊肿。我们在患者6的家族中鉴定出三个新的杂合错义突变, 包括APOB中的c.12614CT(p.Pro4205Leu)、CILP2中的c.160GC(p.Glu54Gln)和PEPD中的c.1199CA(p.Ala400Glu)。 结论: 首次在患有DKA、高三酰甘油血症和AP的三联征患者中发现了三个新的杂合错义突变, 包括APOB中的c.12614CT(p.Pro4205Leu)、CILP2中的c.160GC(p.Glu54Gln)和PEPD中的c.1199CA(p.Ala400Glu)。血浆置换、水合作用和胰岛素治疗相结合可能对这些患者具有最大的临床益处。.

In this work, we report the bidirectional photoresponse of the carbon-based graphdiyne/graphene (GDY/Gr) heterojunction photodetector toward the optical logic gates. The uniform GDY film was facilely grown on the surface of Gr serving as the template. Under mV-level bias, the resultant Au/GDY/Gr/Au devices manifest the bidirectional photoresponse to a broad wavelength range with rapid response time, namely, positive and negative photoresponse under negative and positive bias, respectively. It can be understood from the viewpoint of the divergence and coincidence between in-plane and external potential. Furthermore, the optically operated logical gates including logic AND, OR, and Exclusive-OR gates have been realized by integrating the fabricated Au/GDY/Gr/Au devices, converting optical signals to electrical signals in real-time. It suggests the potential application of GDY/Gr heterojunction toward visible-NIR detector as well as the stable optical logic units.

This paper proposes a UAV platform that autonomously detects, hunts, and takes down other small UAVs in GPS-denied environments. The platform detects, tracks, and follows another drone within its sensor range using a pre-trained machine learning model. We collect and generate a 58,647-image dataset and use it to train a Tiny YOLO detection algorithm. This algorithm combined with a simple visual-servoing approach was validated on a physical platform. Our platform was able to successfully track and follow a target drone at an estimated speed of 1.5 m/s. Performance was limited by the detection algorithm's 77% accuracy in cluttered environments and the frame rate of eight frames per second along with the field of view of the camera.

Small nucleolar RNA host gene 12 (SNHG12) has been demonstrated to be a long noncoding RNA (lncRNA) that facilitates the progression of several solid malignant tumors. However, whether the expression level of SNHG12 in solid malignant tumors is associated with patients prognosis have not been investigated.We systematically searched PubMed, EMBASE and Cochrane Library from Jan 1, 1950 to Mar 24, 2020 for randomized controlled trials published in English on SNHG12 expression in solid malignant tumors. We used the Newcastle-Ottawa Scale to assess the quality of articles. The HRs and 95%CI that extracted from Kaplan-Meier curves were used to perform the forest plot using a fixed-effects model. The meta-analysis was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines.Thirteen articles containing 821 patients were included in this systematic review and meta-analysis. The result showed that high lncRNA SNHG12 expression is significantly associated with poor overall survival (OS) (HR = 1.94, 95% CI: 1.56-2.41, P .001) and the studies are lack of statistically significant heterogeneity (P= .878, I = 0.0%). Beggs plot and Eggers test were applied to testify no publication bias existence in these studies. Subgroup analyses were performed and the result showed that TNM stage, lymph node metastasis and tumor type can influence the patients outcome, while there was no significantly correlation between SNHG12 expression and gender.The systematical review and meta-analysis synthetically analyzed 13 articles including 821 patients with ten types of solid malignant tumors, concluding that higher lncRNA SNHG12 expression is significantly associated with worse clinical prognosis.

To evaluate the efficacy and safety of pylorus-preserving pancreatico-duodenectomy(PPPD) in the treatment of periampullary adenocarcinoma by using Meta-analysis.From January 1, 1980 to November 8, 2013, the articles of randomized controlled trials (RCTs) about PPPD versus PD in the treatment of periampullary adenocarcinoma were collected from Cochrane Libriary, Embase, PubMed, Ovid, Web of science and CBM etc. The methodological quality of the included studies was evaluated according to Cochrane system review valuator handbook of risk of bias standards. Meta-analysis was performed by RevMan 5.2 software.Seven RCTs were enrolled in the meta-analysis. Compared to PD group, PPPD group was associated with significantly less intraoperative blood loss (MD=-200.10, 95% CI:-400.66 to 0.46, P=0.05), shorter operation time (MD=-46.55, 95% CI:-91.02 to -2.07, P=0.04), and less postoperative blood transfusion (MD=-0.89, 95% CI:-1.59 to -0.19, P=0.01). There were no significant differences between the PPPD and PD group in pancreatic fistula, biliary fistula, intestinal fistula, abdominal abscess, postoperative bleeding, wound infection, relaparotomy, mortality and survival rate(all P0.05).PPPD in the treatment of the periampullary adenocarcinoma is safe and effective with similar survival of PD surgery. PPPD can reduce operative time, intraoperative blood loss, transfusion and does not increase the surgery complications as compared to PD.

Homeobox B9 (HOXB9) is proposed to be involved in tumor angiogenesis and metastasis. We investigated the role of HOXB9 in the progression of colon cancer.HOXB9 expression was investigated by immunohistochemically and Western blotting in 128 colon cancer patients and the results were analyzed statistically associated with clinicopathological data and survival of the patients. The effect of HOXB9 on cell invasion and metastases abilities were analyzed in vitro and in vivo.HOXB9 is overexpressed in colon cancer tissues and significantly correlated with metastasis and poor survival of patients (P0.05, respectively). Additionally, high levels of expression of HOXB9 were observed in metastatic lymph nodes. The down-regulation of HOXB9 expression can inhibit the migration and invasive ability of colon cancer cells, while exogenous expression of HOXB9 in colon cancer cells enhanced cell migration and invasiveness. Moreover, stable knockdown of HOXB9 reduced the liver and lung metastasis of colon cancer in vivo.HOXB9 may play an important role in the invasion and metastasis of colon cancer cells and may be a useful biomarker for metastasis and prognostic of colon cancer.

To compare the effectiveness of vocal cord cyst excision with electronic laryngoscope (EL) and self-retaining laryngoscope (SRM).Nightly-two patients, diagnosed as vocal cord cyst with strobolaryngoscope or electronic laryngoscope, were randomly divided into two groups. One group was 48 cases treated with electronic laryngoscope and another group was 44 cases treated with self-retaining laryngoscope. Electronic laryngoscopy examination and voice function assessments were performed to all patients, preoperatively and postoperatively at one week, three months and six months.One failed cases under SRM, difficult exposure of glottic portion, were treated under EL. The recurrence rate of the two groups was of no statistical significance in three months after operation. Voice function assessment of the two groups was of no statistical significance at one week, three months and six months after operation.The operation under electronic laryngoscope is a minimal invasive procedure to the laryngeal mucosa. Electronic laryngoscope had advantages such as clear view, accurate operation. Furthermore, it can be used for those that could not be treated under self-retaining laryngoscope.