13 results on '"plasma microRNA"'
Search Results
2. Increased Expression of Plasma miRNA-320a and let-7b-5p in Heroin-Dependent Patients and Its Clinical Significance
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Haixiong Liu, Wenjin Xu, Jiying Feng, Hong Ma, Jianbin Zhang, Xiaohu Xie, Dingding Zhuang, Wenwen Shen, Huifen Liu, and Wenhua Zhou
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0301 basic medicine ,Oncology ,medicine.medical_specialty ,RC435-571 ,Disease ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Clinical significance ,plasma microRNA ,Bipolar disorder ,Original Research ,drug abuse ,Psychiatry ,Receiver operating characteristic ,business.industry ,Multiple sclerosis ,medicine.disease ,Substance abuse ,Psychiatry and Mental health ,030104 developmental biology ,Schizophrenia ,biomarker ,Biomarker (medicine) ,addiction ,heroin ,business ,030217 neurology & neurosurgery - Abstract
Heroin use disorder is a chronic and relapsing disease that induces persistent changes in the brain. The diagnoses of heroin use disorders are mainly based on subjective reports and no valid biomarkers available. Recent researches have revealed that circulating miRNAs are useful non-invasive biomarkers for diagnosing brain diseases such as Alzheimer's disease, multiple sclerosis, schizophrenia, and bipolar disorder. However, studies on circulating miRNAs for the diagnosis of heroin use disorders are rarely reported. In this study, we investigated the differential expression of plasma miRNAs in 57 heroin-dependent patients. Based on literature research and microarray analysis, two candidate miRNAs, miR-320a and let-7b-5p, were selected and analyzed by quantitative real-time RT-PCR. The results showed miR-320a and let-7b were significantly upregulated in plasma of the heroin-dependent patients compared to that in healthy controls. The area under curves (AUCs) of receiver operating characteristic (ROC) curves of miR-320a and let-7b-5p were 0.748 and 0.758, respectively. The sensitivities of miR-320a and let-7b-5p were 71.9 and 70.2%, while the specificities of miR-320a and let-7b-5p were 76.1 and 78.3%, respectively. The combination of these two miRNAs predicted heron dependence with an AUC of 0.782 (95% CI 0.687–0.876), with 73.7% sensitivity and 82.6% specificity. Our findings suggest a potential use for circulating miRNAs as biomarkers for the diagnosis of heroin abuse.
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- 2021
3. High-Throughput RNA Sequencing Analysis of Plasma Samples Reveals Circulating microRNA Signatures with Biomarker Potential in Dengue Disease Progression
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Vishnampettai G. Ramachandran, Vikas Sood, Bhaswati Bandyopadhyay, Sudhanshu Vrati, Abhay Deep Pandey, Jaya Saini, Shukla Das, and Arup Banerjee
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0301 basic medicine ,Small RNA ,Physiology ,Dengue virus ,medicine.disease_cause ,Biochemistry ,Microbiology ,Dengue fever ,Host-Microbe Biology ,03 medical and health sciences ,0302 clinical medicine ,microRNA ,Genetics ,Medicine ,plasma microRNA ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics ,Respiratory distress ,business.industry ,RNA sequencing ,medicine.disease ,dengue ,QR1-502 ,Fold change ,Computer Science Applications ,circulating miRNA ,Circulating MicroRNA ,030104 developmental biology ,030220 oncology & carcinogenesis ,Modeling and Simulation ,Immunology ,Biomarker (medicine) ,business ,Research Article - Abstract
Dengue virus (DENV) infection usually causes dengue fever (DF) with flu-like illness affecting infants, young children, and adults. The DF occasionally evolves into a potentially lethal complication called dengue severe (DS) leading to a rapid fall in platelet count along with plasma leakage, fluid accumulation, respiratory distress, and severe bleeding. The diverse clinical spectrum of dengue disease, as well as its significant similarity to other febrile viral illnesses, makes early identification more challenging in this high-risk group. microRNAs (miRNAs) are small (∼19 to 21 nucleotides [nt] in length), noncoding RNAs, extremely stable and easily detectable in the plasma; thus, they have potential as biomarkers for diagnosing and monitoring human diseases. This study provides a comprehensive analysis of miRNAs circulating in plasma of dengue virus-infected patients and identifies the miRNA signatures that have biomarker potential for dengue infection and disease progression., The circulating microRNA (miRNA) profile has been widely used for identifying potential biomarkers against viral infections. However, data on circulating microRNA expression patterns in dengue patients are scanty. Considering the impact of severity caused by dengue infection, circulating miRNA profiles in plasma of dengue patients may prove to be valuable for developing early prognostic markers for the disease severity. Here, we described an in-depth analytical study of small RNA sequencing data obtained from the plasma of 39 dengue patients. Integrating bioinformatics and in vitro studies, we identified differentially expressed miRNAs (DEMs) (log2 fold change ≥1.5, P
- Published
- 2020
4. A Traditional Korean Diet Alters the Expression of Circulating MicroRNAs Linked to Diabetes Mellitus in a Pilot Trial
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Hye Jeong Yang, Dae Young Kwon, Seon-Joo Park, Kyong-chol Kim, Phil-Kyung Shin, Sukyung Chun, Hae-Jeung Lee, Myung Sunny Kim, Min Jung Kim, Soon Hee Kim, and Sang-Woon Choi
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0301 basic medicine ,Acute coronary syndrome ,Saliva ,Diet therapy ,Korean diet ,Physiology ,lcsh:TX341-641 ,Pilot Projects ,Disease ,Article ,salivary microRNA ,03 medical and health sciences ,0302 clinical medicine ,Diabetes mellitus ,Republic of Korea ,Medicine ,Humans ,plasma microRNA ,Circulating MicroRNA ,Nutrition and Dietetics ,business.industry ,Fatty liver ,medicine.disease ,Obesity ,Diet ,030104 developmental biology ,030220 oncology & carcinogenesis ,diabetes mellitus ,Female ,business ,lcsh:Nutrition. Foods and food supply ,Biomarkers ,Food Science - Abstract
The traditional Korean diet (K-diet) is considered to be healthy and circulating microRNAs (miRs) have been proposed as useful markers or targets in diet therapy. We, therefore, investigated the metabolic influence of the K-diet by evaluating the expression of plasma and salivary miRs. Ten women aged 50 to 60 years were divided into either a K-diet or control diet (a Westernized Korean diet) group. Subjects were housed in a metabolic unit-like condition during the two-week dietary intervention. Blood and saliva samples were collected before and after the intervention, and changes in circulating miRs were screened by an miR array and validated by individual RT-qPCRs. In the K-diet group, eight plasma miRs were down-regulated by array (p <, 0.05), out of which two miRs linked to diabetes mellitus, hsa-miR26a-5p and hsa-miR126-3p, were validated (p <, 0.05). Among five down-regulated salivary miRs, hsa-miR-92-3p and hsa-miR-122a-5p were validated, which are associated with diabetes mellitus, acute coronary syndrome and non-alcoholic fatty liver disease. In the control diet group, validated were down-regulated plasma hsa-miR-25-3p and salivary hsa-miR-31-5p, which are associated with diabetes mellitus, adipogenesis and obesity. The K-diet may influence the metabolic conditions associated with diabetes mellitus, as evidenced by changes in circulating miRs, putative biomarkers for K-diet.
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- 2020
5. Integrating clinical and biological prognostic biomarkers in patients with advanced NSCLC treated with immunotherapy: the DEMo score system
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Marta Brambilla, Roberto Ferrara, Giulia Galli, Giuseppe Viscardi, Claudia Proto, Diego Signorelli, Mavis Mensah, Alessandro De Toma, Benedetta Trevisan, Arsela Prelaj, Francesco Trovò, Riccardo Lobefaro, Valter Torri, Gabriella Sozzi, Marina Chiara Garassino, Giuseppe Lo Russo, and Mattia Boeri
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Oncology ,Complete data ,medicine.medical_specialty ,medicine.medical_treatment ,non-small cell lung cancer (NSCLC) ,Non-small cell lung cancer (NSCLC) ,Metastasis ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Medicine ,In patient ,030212 general & internal medicine ,plasma microRNA ,Lung cancer ,business.industry ,Immunotherapy ,medicine.disease ,Editorial ,030220 oncology & carcinogenesis ,Cohort ,Biomarker (medicine) ,biomarker ,Original Article ,prognosis ,immunotherapy ,business - Abstract
BACKGROUND: Several biomarkers have been separately described to select patients for immunotherapy (IO), but few studies integrate these markers. Di Maio, EPSILoN and the plasma microRNA signature classifier (MSC), are three different clinico, biochemical and molecular markers able to independently predict prognosis in non-small cell lung cancer (NSCLC). METHODS: Complete data such as sex, histology, ECOG-PS, stage, smoking status, presence of liver metastasis, LDH and neutrophils-to-lymphocyte ratio were collected to generate Di Maio and EPSILoN. The MSC risk level was prospectively assessed in plasma samples collected at baseline IO. The 3 markers were integrated into the DEMo score system prospectively tested in a cohort of 200 advanced NSCLC patients treated with IO. Endpoints were overall survival (OS), progression-free survival (PFS) and overall response rate (ORR). RESULTS: DEMo separated patients in 7-risk groups whose median OS had a trend ranging from 29.7 to 1.5 months (P
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- 2020
6. Assessment of plasma microRNAs in congenital intestinal malrotation
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Xiurui Lv, Xinhe Sun, Changgui Lu, Lingling Zhou, Hongxing Li, and Huan Chen
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Male ,Cancer Research ,intestinal malrotation ,Bioinformatics ,Biochemistry ,microRNA ,Genetics ,Medicine ,Humans ,plasma microRNA ,Molecular Biology ,Gene ,Receiver operating characteristic ,Oncogene ,business.industry ,Sequence Analysis, RNA ,Gene Expression Profiling ,Infant, Newborn ,Computational Biology ,High-Throughput Nucleotide Sequencing ,Articles ,medicine.disease ,Molecular medicine ,Metal ion transmembrane transporter activity ,Volvulus ,MicroRNAs ,Early Diagnosis ,Oncology ,Intestinal malrotation ,Molecular Medicine ,biomarker ,Female ,business ,Biomarkers ,Intestinal Volvulus - Abstract
Intestinal malrotation in newborns often requires urgent surgical treatment, especially in the presence of volvulus. Therefore, early‑stage diagnosis is critical. In the present study, differentially expressed plasma microRNAs (miRNAs) were screened for in patients with intestinal malrotation using high‑throughput Illumina sequencing, and validated using reverse transcription‑quantitative PCR. Receiver operating characteristic curve (ROC) analysis was conducted to evaluate their specificity, sensitivity and assess their diagnostic value for intestinal malrotation. Bioinformatics analysis was performed to investigate the functions associated with the dysregulated miRNAs. A profile consisting of 28 differentially expressed plasma miRNAs was obtained, of which nine were verified to exhibit significantly altered expression. According to a ROC analysis, four of these could represent novel early‑stage, non‑invasive biomarkers for intestinal malrotation. Bioinformatics analysis demonstrated that the differentially expressed miRNAs were predominantly involved in 'metal ion transmembrane transporter activity' and 'calcium‑dependent protein binding', which may be related to the 'endocytosis' pathway. In conclusion, significantly differentially expressed plasma miRNAs were identified in congenital intestinal malrotation and their potential roles were described. These differentially expressed miRNAs may serve as biomarkers of intestinal malrotation and improve early diagnosis for this condition.
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- 2019
7. Assessment of differentially expressed plasma microRNAs in nonsyndromic cleft palate and nonsyndromic cleft lip with cleft palate
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Qian Li, Bei Zhou, Hui Yan, Jingyun Li, Jun Li, Ling Chen, Jijun Zou, and Yanli Gao
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Male ,0301 basic medicine ,Cleft Lip ,Dentistry ,miRNA microarray ,Real-Time Polymerase Chain Reaction ,Bioinformatics ,Regulatory molecules ,03 medical and health sciences ,microRNA ,Humans ,Medicine ,plasma microRNA ,nonsyndromic cleft lip with cleft palate ,Plasma samples ,business.industry ,Wnt signaling pathway ,Infant ,Pathway analysis ,Cleft Palate ,MicroRNAs ,Hedgehog signalling ,Gene Ontology ,030104 developmental biology ,Oncology ,Female ,nonsyndromic cleft palate ,Mirna microarray ,business ,Signalling pathways ,Signal Transduction ,Research Paper - Abstract
// Jingyun Li 1, * , Jijun Zou 2, * , Qian Li 1 , Ling Chen 1 , Yanli Gao 1 , Hui Yan 1 , Bei Zhou 1 , Jun Li 1 1 State Key Laboratory of Reproductive Medicine, Department of Plastic and Cosmetic Surgery, Maternal and Child Health Medical Institute, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China 2 Department of Burns and Plastic Surgery, Children’s Hospital of Nanjing Medical University, Nanjing 210008, China * These authors contributed equally to this work Correspondence to: Jun Li, email: drlijun123@163.com Keywords: nonsyndromic cleft palate, nonsyndromic cleft lip with cleft palate, plasma microRNA, miRNA microarray Received: September 30, 2016 Accepted: November 05, 2016 Published: November 16, 2016 ABSTRACT Plasma microRNAs (miRNAs) have recently emerged as a new class of regulatory molecules that influence many biological functions. However, the expression profile of plasma microRNAs in nonsyndromic cleft palate (NSCP) or nonsyndromic cleft lip with cleft palate (NSCLP) remains poorly investigated. In this study, we used Agilent human miRNA microarray chips to monitor miRNA levels in three NSCP plasma samples (mixed as the CP group), three NSCLP plasma samples (mixed as the CLP group) and three normal plasma samples (mixed as the Control group). Six selected plasma miRNAs were validated in samples from an additional 16 CP, 33 CLP and 8 healthy children using qRT-PCR. Using Venn diagrams, distinct and overlapping dysregulated miRNAs were identified. Their respective target genes were further assessed using gene ontology and pathway analysis. The results show that distinct or overlapping biological processes and signalling pathways were involved in CP and CLP. Our study showed that the common key gene targets reflected functional relationships to the Notch, Wnt, phosphatidylinositol and Hedgehog signalling pathways. Further studies should examine the mechanism of the potential target genes, which may provide new avenues for future clinical prevention and therapy.
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- 2016
8. Low levels of tumour suppressor miR-655 in plasma contribute to lymphatic progression and poor outcomes in oesophageal squamous cell carcinoma
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Kazuma Okamoto, Toshiyuki Kosuga, Hitoshi Fujiwara, Taisuke Imamura, Shuhei Komatsu, Eigo Otsuji, Jun Kiuchi, Tomohiro Arita, Keiji Nishibeppu, Katsutoshi Shoda, Daisuke Ichikawa, Atsushi Shiozaki, and Hirotaka Konishi
- Subjects
Male ,0301 basic medicine ,Cancer Research ,Epithelial-Mesenchymal Transition ,Esophageal Neoplasms ,Lymphovascular invasion ,Biology ,lcsh:RC254-282 ,Mouse model ,law.invention ,03 medical and health sciences ,Subcutaneous injection ,0302 clinical medicine ,Cell Movement ,law ,microRNA ,Humans ,Genes, Tumor Suppressor ,Risk factor ,Letter to the Editor ,Therapeutic agent ,Cell Proliferation ,Lymph node metastasis ,Cell growth ,Plasma microRNA ,Biomarker ,Middle Aged ,Oesophageal squamous cell carcinoma ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,MicroRNAs ,030104 developmental biology ,Lymphatic system ,Oncology ,Lymphatic Metastasis ,030220 oncology & carcinogenesis ,Disease Progression ,Cancer research ,Molecular Medicine ,Biomarker (medicine) ,Suppressor ,Female ,Esophageal Squamous Cell Carcinoma - Abstract
Recent studies identified that low levels of tumour suppressor microRNAs (miRNAs) in plasma/serum relate to tumour progression and poor outcomes in cancers. We selected six candidates (miR-126, 133b, 143, 203, 338-3p, 655) of tumour suppressor miRNAs in oesophageal squamous cell carcinoma (ESCC) by a systematic review of NCBI database. Of these, miR-655 levels were significantly down-regulated in plasma of ESCC patients compared to healthy volunteers by test- and validation-scale analyses. Low levels of plasma miR-655 were significantly associated with lymphatic invasion, lymph node metastasis and advanced stage. Univariate and multivariate analysis revealed that the low level of plasma miR-655 was an independent risk factor of lymphatic progression and a poor prognostic factor. Overexpression of miR-655 in ESCC cells inhibited cell proliferation, migration, invasion and epithelial-mesenchymal transition. Increased plasma miR-655 levels by the subcutaneous injection significantly inhibited lymph node metastasis in mice. Low levels of miR-655 in plasma relate to lymphatic progression and poor outcomes, and the restoration of the plasma miR-655 levels might inhibit tumour and lymphatic progression in ESCC. Electronic supplementary material The online version of this article (10.1186/s12943-018-0929-3) contains supplementary material, which is available to authorized users.
- Published
- 2019
9. MicroRNAs in heart failure
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ACUTE MYOCARDIAL-INFARCTION ,PLASMA MICRORNA ,Heart failure ,ADULT HEARTS ,EJECTION FRACTION ,HYPERTROPHY ,MicroRNAs ,Circulation ,CIRCULATING MICRORNAS ,CARDIOVASCULAR-DISEASE ,SIGNATURES DIFFERENTIATE ,FIBROSIS ,CARDIAC RESYNCHRONIZATION THERAPY ,Antimir - Abstract
MicroRNAs (miRNAs) are increasingly recognized to play important roles in cardiovascular diseases, including heart failure. These small, non-coding RNAs have been identified in tissue and are involved in several pathophysiological processes related to heart failure, such as cardiac fibrosis and hypertrophy. As a result, miRNAs have become interesting novel drug targets, leading to the development of miRNA mimics and antimirs. MicroRNAs are also detected in the circulation, and are proposed as potential diagnostic and prognostic biomarkers in heart failure. However, their role and function in the circulation remains to be resolved. Here, we review the potential roles of miRNAs as circulating biomarkers and as targets for therapy.
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- 2016
10. Circulating microRNAs are associated with early childhood obesity: results of the I.Family Study
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Ronja Foraita, Alfonso Siani, Pasquale De Luca, Fabio Lauria, Dénes Molnár, Giuseppe Iacomino, Toomas Veidebaum, Wolfgang Ahrens, Pasquale Marena, Michael Tornaritis, Kathrin Günther, Antonella Venezia, Luis A. Moreno, Lauren Lissner, Paola Russo, and Stefaan De Henauw
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0301 basic medicine ,Oncology ,obesity ,medicine.medical_specialty ,lcsh:QH426-470 ,PLASMA MICRORNA ,Endocrinology, Diabetes and Metabolism ,Metabolic disorders ,lcsh:TX341-641 ,030209 endocrinology & metabolism ,Disease ,Clinical nutrition ,Overweight ,Childhood obesity ,Childhood overweight ,low-grade ,03 medical and health sciences ,0302 clinical medicine ,Diabetes mellitus ,Internal medicine ,Medicine and Health Sciences ,Genetics ,QUALITY ,Medicine ,2. Zero hunger ,business.industry ,Research ,SIGNATURE ,Biology and Life Sciences ,EXPRESSION PROFILE ,Biomarker ,medicine.disease ,Obesity ,Childhood overweight/low-grade obesity ,Circulating miRNAs ,3. Good health ,BODY-MASS INDEX ,lcsh:Genetics ,Circulating MicroRNA ,030104 developmental biology ,MIRNAS ,medicine.symptom ,business ,lcsh:Nutrition. Foods and food supply ,Dyslipidemia - Abstract
Background: Nearly 10 years ago, the World Health Organization reported the increasing prevalence of overweight and obesity worldwide as a challenge for public health due to the associated adverse consequences. Epidemiological studies established a firm relationship between an elevated body mass index and chronic conditions such as diabetes, dyslipidemia, hypertension, heart disease, non-alcoholic fatty liver disease, and some types of cancer. Omic studies demonstrated that microRNA (miRNA) profile changes in tissues correlate with a number of diseases, including obesity. Recent studies showed a remarkable stability of miRNAs also in blood, emphasizing their potential as theranostic agents for a variety of disorders and conditions. A number of miRNAs enriched in homeostasis of obesity and metabolic disorders have been characterized in previous researches.Aim: This work was finalized to investigate the differential circulating miRNAs signature in early childhood obesity. Our cross-sectional study analyzed the signature of circulating miRNAs in plasma samples of normal weight (n = 159) and overweight/obese (n = 149) children and adolescents participating to the I.Family study, an EC-funded study finalized to investigate the etiology of overweight, obesity and related disorders and the determinants of food choice, lifestyle, and related health outcomes in children and adolescents of eight European countries (www.ifamilystudy.eu).Results: Differences in miRNA signature with respect to anthropometric and biochemical variables were analyzed. A high degree of variability in levels of circulating miRNAs was identified among children from different countries, in line with recent reports supporting the hypothesis that these molecules are likewise affected by environmental and lifestyle factors. A panel of miRNAs differentially expressed in overweight/low-grade obesity children was characterized (miR-551a and miR-501-5p resulted upregulated; miR-10b-5p, miR-191-3p, miR-215-5p, and miR-874-3p resulted downregulated). ROC curves were also constructed for experimentally confirmed miRNAs. Single miRNAs generally exhibited low AUC values with the highest values for miR-874-3p and miR-501-5p which in combination provided an interesting value (AUC = 0.782). Pearson's analysis confirmed that miR-10b-5p, miR-215-5p, miR-501-5p, miR-551a, and miR-874-3p significantly correlated with BMI z-score. Molecular interactions of obesity-associated miRNAs were also predicted by bioinformatics tools.Conclusions: Our work showed that several circulating miRNAs are differentially represented in overweight/low-grade obesity children and adolescents. Although causal pathways cannot be firmly inferred, it is conceivable that circulating miRNAs may be new biomarkers of early childhood obesity.Trial registration: ISRCTN, ISRCTN62310987. Registered 23/02/2018 - Retrospectively registered.
- Published
- 2018
11. Integrated assessment of differentially expressed plasma microRNAs in subtypes of nonsyndromic orofacial clefts
- Author
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Jun Yan, Weimin Shen, Tao Han, Ni Wu, and Jijun Zou
- Subjects
0301 basic medicine ,Cleft Lip ,miRNA microarray ,Bioinformatics ,Epigenesis, Genetic ,cleft lip with palate ,03 medical and health sciences ,microRNA ,Medicine ,Humans ,plasma microRNA ,Gene ,Epigenesis ,cleft palate ,Mechanism (biology) ,business.industry ,Wnt signaling pathway ,Brain ,General Medicine ,Clinical Trial/Experimental Study ,Pathway analysis ,Microarray Analysis ,Hedgehog signaling pathway ,MicroRNAs ,030104 developmental biology ,ComputingMethodologies_DOCUMENTANDTEXTPROCESSING ,nonsyndromic cleft lip ,Signal transduction ,business ,Signal Transduction ,Research Article - Abstract
Supplemental Digital Content is available in the text, Background: Orofacial clefts include cleft lip only (CLO), cleft palate only (CPO), and cleft lip with palate (CLP). Previously, we reported the expression profile of plasma microRNAs in CLO, CPO, and CLP, respectively. However, the interaction of each subtype remains poorly investigated. Methods: In this study, we integrated the expression profiles of plasma miRNAs in these 3 subtypes, and assessed the distinct and overlapping dysregulated miRNAs using Venn diagrams. Their respective target genes reported in the literature were further analyzed using pathway analysis. Results and conclusion: The results showed that distinct or overlapping signaling pathways were involved in CLO, CPO, and CLP. The common key gene targets reflected functional relationships to the Wnt, Notch, TGF-beta, and Hedgehog signaling pathways. Further studies should examine the mechanism of the potential target genes, which may provide new avenues for future clinical prevention and therapy.
- Published
- 2018
12. MicroRNA in atherothromobosis: is it useful as a disease marker?
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Nobuyuki Ohte, Satoshi Fujii, Yasuaki Dohi, and Tomonori Sugiura
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0301 basic medicine ,medicine.medical_specialty ,Endothelium ,Review ,Coronary artery disease ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Internal medicine ,Hyperlipidemia ,Medicine ,Myocardial infarction ,Reactive hyperemia ,Angiology ,business.industry ,Cholesterol ,Smoking ,Plasma microRNA ,Hematology ,medicine.disease ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,030220 oncology & carcinogenesis ,LDL cholesterol ,Cardiology ,business ,Lipoprotein - Abstract
Atherosclerosis is one of the major causes of death. Data from animal experiments suggest that atherosclerosis involves an inflammatory process of the vascular wall under hyperlipidemia. Atherothrombosis can become a serious complication of atherosclerosis leading to acute cardiovascular events such as myocardial infarction and stroke. Clinical applications to use this knowledge remain scarce. The plasma levels of vascular endothelium-enriched microRNA (miRNAs) in patients with atherosclerotic vascular disease could serve as a disease marker. In our laboratory vascular endothelium-enriched miRNA (miR-126) level was analyzed using quantitative RT polymerase chain reaction analysis (qRT-PCR) in plasma from patients with suspected coronary artery disease (CAD) according to the chest symptom or findings of electrocardiogram, or middle-aged male smokers. Endothelial function for peripheral small vessels was assessed using End-PAT 2000 and expressed as reactive hyperemia peripheral arterial tonometry (RH-PAT) index. In patients with suspected CAD miR-126 was not significantly changed in CAD patients. However, miR-126 was decreased in CAD patients who also have high levels of low-density lipoprotein (LDL) cholesterol. Interestingly, miR-126 was increased when LDL cholesterol was high in patients who did not have evident CAD on coronary angiography even though they have risk factors for CAD. In smokers serum cotinine levels were inversely correlated with endothelial function expressed as RH-PAT index and positively correlated with levels of metabolic parameters such as non-high-density lipoprotein (HDL) cholesterol and insulin resistance. More than half of the smokers could not completely attain smoking cessation and, thus, the RH-PAT index was not improved 8 weeks after the instruction of smoking cessation. However, changes in the RH-PAT index showed a significant correlation with those in systolic blood pressure. In smokers who completely attained smoking cessation, both RH-PAT index and plasma miR-126 values were increased. Thus, among patients with suspected CAD or subjects with coronary risk factors plasma levels of endothelium-enriched circulating miR-126 could be substantially altered. The results suggest a potential usefulness of miR-126 as a sensitive biomarker in assessing endothelial damage. Measurement of microRNA may serve as a useful tool for laboratory assays to determine high-risk patients for atherothromobotic vascular diseases.
- Published
- 2016
13. Increased microRNA-34c abundance in Alzheimer's disease circulating blood plasma
- Author
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Samantha Jenkins, Vikranth Shetty, Timothy W. Jones, Eugenia Wang, Shephali Bhatnagar, Zongfei Yuan, Hyman M. Schipper, and Howard Chertkow
- Subjects
medicine.medical_specialty ,Peripheral blood mononuclear cell ,lcsh:RC321-571 ,Cellular and Molecular Neuroscience ,Internal medicine ,Blood plasma ,microRNA ,Medicine ,Original Research Article ,plasma microRNA ,lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry ,Molecular Biology ,Gene ,Receiver operating characteristic ,synaptic dysfunction ,neuronal survival ,business.industry ,Area under the curve ,Transfection ,Alzheimer's disease ,peripheral blood mononuclear cells ,Circulating MicroRNA ,Endocrinology ,Immunology ,peripheral blood mononuclear cells (PBMC) ,business ,miR-34c ,miR-34a ,Neuroscience - Abstract
Circulating microRNAs, present either in the cellular component, peripheral blood mononuclear cells (PBMC), or in cell-free plasma, have emerged as biomarkers for age-dependent systemic, disease-associated changes in many organs. Previously, we have shown that microRNA (miR)-34a is increased in circulating PBMC of Alzheimer's disease (AD) patients. In the present study, we show that this microRNA's sister, miR-34c, exhibits even greater increase in both cellular and plasma components of AD circulating blood samples, compared to normal age-matched controls. Statistical analysis shows the accuracy of levels of miR-34c assayed by receiver operating characteristic (ROC) analysis: the area under the curve is 0.99 (p < 0.0001) and the 95% confidence level extends from 0.97 to 1. Pearson correlation between miR-34c levels and mild and moderate AD, as defined by the mini-mental state examination (MMSE), shows an r-value of −0.7, suggesting a relatively strong inverse relationship between the two parameters. These data show that plasma levels of microRNA 34c are much more prominent in AD than those of its sister, miR-34a, or than its own level in PBMC. Transfection studies show that miR-34c, as does its sister miR-34a, represses the expression of several selected genes involved in cell survival and oxidative defense pathways, such as Bcl2, SIRT1, and others, in cultured cells. Taken together, our results indicate that increased levels of miR-34c in both PBMC and plasma may reflect changes in circulating blood samples in AD patients, compared to age-matched normal controls.
- Published
- 2013
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