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INTRODUCTION: Osseodensification is an innovative technique in dentistry to create osteotomies in bone to enhance bone density. Developed by Huwais in 2013, this method has been used to increase the primary stability around dental implants, help in indirect sinus lifts and achieve desired expansion in bone. AIM: The main objective of this study was to examine the effects of osseodensification-induced bone modification in mouse ex vivo cultures under dynamic conditions. MATERIALS AND METHODS: Thirty calvaria were surgically extracted from 7-9 days old mice and divided into groups. Densah burs were utilized to create the defects. Clockwise action of the bur produced conventional osteotomies whereas counter-clockwise action created osseodensification effects. Photomicrographs were taken on days 7,14 and 28. Image J software was used to trace the defects and all data were transferred into Microsoft Excel to generate graphs. Statistical analysis and tests of association were done using SPSS software. RESULTS: Increased defect closure was evident in the “Osseodensification” group compared to the “Conventional Osteotomy” group. Defect closure was highest in the first week. There was no statistical significance between groups in original defect size (P-value: 0.6097). Comparing both groups, defect closure was statistically significant on day 7 (P-value: 0.0313). CONCLUSION: Osseodensification has proven to be superior to conventional osteotomies in terms of healing around bony defects and enhancing the primary stability of dental implants. Dynamic conditions during the initial phase of wound healing could hinder the healing cascade and result in a delay of the normal healing process.

BACKGROUND: Recently osseodensification has been introduced as a novel approach to management of the recipient site. The concept had been described in 2013 by Huwais which has revolutionized the way we approach an osteotomy site as we may obtain densification of the bone rather than its complete removal. AIM: We hypothesize that proper manipulation of the recipient site will induce cellular activities to accelerate new bone formation. We compared bone formation in a critical defect created by the osseodensification method or regular osteotomy under ex-vivo static calvarial culture. MATERIALS AND METHODS: Under sterile conditions, calvaria from 7-9-day-old neonatal CD-1 mice (n = 15) were dissected and trimmed. Densah™ burs were used to create 2.0 mm diameter defects. Clockwise rotation of the bur produced "Conventional Osteotomy," whereas counter-clockwise rotation created "Osseodensification." Five randomly selected calvaria halves for control and test groups were used to evaluate morphological changes, at 7, 14, and 28 days utilizing the Image J software. Statistical analyses were performed using SPSS software. RESULTS: Defect closure was significantly greater in the osseodensification group compared to the conventional group at post-operative day7 (p = 0.028), day 14 (p = 0.046) and day 28 (p=0.015). The original defects in both groups were not significantly different. CONCLUSION: Results showed that osseodensification lead to faster wound healing. Clinical studies have shown that osseodensification leads to better bone density around implants. These outcomes suggest that the compressed edge of a bone defect can accelerate the healing cascade by increasing cellular activity.