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To achieve breakthrough innovation, firms often need to acquire complementary knowledge from cross-industry actors in the innovation ecosystem, where unique and supermodular complementarities make knowledge exchange essential for value co-creation. This study examines the impact of the acquisition of knowledge transfer on breakthrough innovation by considering the complementors of unique and supermodular complementarity. We draw on data from 51 firms in the Chinese information and communication technology sector and apply the fuzzy set qualitative comparative analysis (fsQCA), in which we adopt factors on the complementor, relationship, and knowledge characteristics to determine the configurations that lead to firms' breakthrough innovation in the innovation ecosystem. The results show that several combinations of conditions drive firms' breakthrough innovation. Unique complementarities require conditions of explicit knowledge and cultural similarity within the realm of knowledge and complementors' characteristics. Conversely, supermodular complementarity underscores the importance of tacit knowledge, along with trust and tie strength within the characteristics of knowledge and relationships. Our study has practical and theoretical implications for complementors seeking breakthrough innovation in non-generic complementarities. • Unique and supermodular complementarities in the innovation ecosystem are heterogeneous. • We examine the acquisition of knowledge transfer's impact on breakthrough innovation to unpack these differences. • The results show that several combinations of knowledge transfer conditions drive focal firms' breakthrough innovation. • Unique complementarities emphasize knowledge specificity and require explicit knowledge and cultural similarity. • Supermodular complementarity underscores an extensive connection, considering trust and tie strength as the core. [ABSTRACT FROM AUTHOR]