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3 results on '"Anderson, Meegan R."'

1. PET/CT Targeted Tissue Sampling Reveals Intravenously Administered HGN194 IgG1 Affects HIV Distribution after Rectal Exposure.

Author

Taylor, Roslyn A., Xiao, Sixia, Carias, Ann M., McRaven, Michael D., Thakkar, Divya N., Araínga, Mariluz, Lorenzo-Redondo, Ramon, Allen, Edward J., Rogers, Kenneth A., Kumarapperuma, Sidath C., Gong, Siqi, Anderson, Meegan R., Thomas, Yanique, Madden, Patrick J., Corti, Davide, Cameroni, Elisabetta, Lanzavecchia, Antonio, Goins, Beth, Fox, Peter, and Villinger, Francois J.

Abstract

Neutralizing monoclonal antibodies hold great potential for prevention of human immunodeficiency virus (HIV) acquisition. IgG is the most abundant antibody in human serum, has a long half-life, and potent effector functions, making it a prime candidate for an HIV prevention therapeutic. We combined Positron Emission Tomography imaging and fluorescent microscopy of 64Cu-labeled, photoactivatable-green fluorescent protein HIV (PA-GFP-BaL) and fluorescently labeled HGN194 IgG1 to determine whether intravenously instilled IgG influences viral interaction with mucosal barriers and viral penetration in colorectal tissue 2 h after rectal viral challenge. Our results show that IgG1 did not alter the number of virions found throughout the colon or viral penetration into the epithelium of the rectum or descending colon. A minor increase in virions was observed in the transverse colon of IgG1 treated animals. Overall, the number of viral particles found in the mesenteric lymph nodes was low. However, IgG1 administration resulted in a significant reduction of virions found in mesenteric lymph nodes. Taken together, our results show that HGN194 IgG1 does not prevent virions from penetrating into the colorectal mucosa but may perturb HIV virion access to the lymphatic system. [ABSTRACT FROM AUTHOR]

Published
2024
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2. Impact of chemokine C–C ligand 27, foreskin anatomy and sexually transmitted infections on HIV-1 target cell availability in adolescent South African males

Author

Gray, Clive M., O'Hagan, Kyle L., Lorenzo-Redondo, Ramon, Olivier, Abraham J., Amu, Sylvie, Chigorimbo-Murefu, Nyaradzo, Harryparsad, Rushil, Sebaa, Shorok, Maziya, Lungile, Dietrich, Janan, Otwombe, Kennedy, Martinson, Neil, Ferrian, Selena, Mkhize, Nonhlanhla N., Lewis, David A., Lang, Dirk, Carias, Ann M., Jaspan, Heather B., Wilson, Douglas P.K., McGilvray, Marcus, Cianci, Gianguido C., Anderson, Meegan R., Dinh, Minh H., Williamson, Anna-Lise, Passmore, Jo-Ann S., Chiodi, Francesca, and Hope, Thomas J.

Abstract

We compared outer and inner foreskin tissue from adolescent males undergoing medical male circumcision to better understand signals that increase HIV target cell availability in the foreskin. We measured chemokine gene expression and the impact of sexually transmitted infections (STIs) on the density and location of T and Langerhans cells. Chemokine C–C ligand 27 (CCL27) was expressed 6.94-fold higher in the inner foreskin when compared with the outer foreskin. We show that the density of CD4+CCR5+cells/mm2was higher in the epithelium of the inner foreskin, regardless of STI status, in parallel with higher CCL27 gene expression. In the presence of STIs, there were higher numbers of CD4+CCR5+cells/mm2cells in the sub-stratum of the outer and inner foreskin with concurrently higher number of CD207+Langerhans cells (LC) in both tissues, with the latter cells being closer to the keratin surface of the outer FS in the presence of an STI. When we tested the ability of exogenous CCL27 to induce T-cell migration in foreskin tissue, CD4 + T cells were able to relocate to the inner foreskin epithelium in response. We provide novel insight into the impact CCL27 and STIs on immune and HIV-1 target cell changes in the foreskin.

Published
2020
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3. Impact of chemokine C–C ligand 27, foreskin anatomy and sexually transmitted infections on HIV-1 target cell availability in adolescent South African males

Author

Gray, Clive M., O’Hagan, Kyle L., Lorenzo-Redondo, Ramon, Olivier, Abraham J., Amu, Sylvie, Chigorimbo-Murefu, Nyaradzo, Harryparsad, Rushil, Sebaa, Shorok, Maziya, Lungile, Dietrich, Janan, Otwombe, Kennedy, Martinson, Neil, Ferrian, Selena, Mkhize, Nonhlanhla N., Lewis, David A., Lang, Dirk, Carias, Ann M., Jaspan, Heather B., Wilson, Douglas P. K., McGilvray, Marcus, Cianci, Gianguido C., Anderson, Meegan R., Dinh, Minh H., Williamson, Anna-Lise, Passmore, Jo-Ann S., Chiodi, Francesca, and Hope, Thomas J.

Abstract

We compared outer and inner foreskin tissue from adolescent males undergoing medical male circumcision to better understand signals that increase HIV target cell availability in the foreskin. We measured chemokine gene expression and the impact of sexually transmitted infections (STIs) on the density and location of T and Langerhans cells. Chemokine C–C ligand 27 (CCL27) was expressed 6.94-fold higher in the inner foreskin when compared with the outer foreskin. We show that the density of CD4+CCR5+cells/mm2was higher in the epithelium of the inner foreskin, regardless of STI status, in parallel with higher CCL27 gene expression. In the presence of STIs, there were higher numbers of CD4+CCR5+cells/mm2cells in the sub-stratum of the outer and inner foreskin with concurrently higher number of CD207+Langerhans cells (LC) in both tissues, with the latter cells being closer to the keratin surface of the outer FS in the presence of an STI. When we tested the ability of exogenous CCL27 to induce T-cell migration in foreskin tissue, CD4 + T cells were able to relocate to the inner foreskin epithelium in response. We provide novel insight into the impact CCL27 and STIs on immune and HIV-1 target cell changes in the foreskin.

Published
2020
Full Text
View/download PDF

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