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In recent years, civil drones have become more and more visible in everyday life. There are numerous reports in the media covering a variety of drone aspects and technical developments. In addition, everyone is used to bird’s-eye views as common features in television, movies and photography. However, little is known about how the public perceives this development. This article reports the results of a representative national study on the acceptance of civilian drones. Overall, a balanced but slightly positive attitude towards civil drones was revealed. Factors analyzed include age, gender, place of residence or interest in technical matters, as well as the individual level of knowledge about the topic. Free verbal associations with the general term drone are described as well as concerns about the usage of civil drones. Concerning different applications of civil drones, results indicate clear approval in Germany for the use of drones in civil protection, rescue missions and research work. However, flights for advertising, leisure and parcel delivery purposes are disagreed with by at least half of those questioned. In the presentation of the results, this article describes social acceptance of civilian drones and thus helps to better understand the perception of civil unmanned aerial vehicles.

Drones may play an essential role in future traffic. As with every innovation, not only is the technical maturity decisive for its success, but also whether the general public will accept it. This paper uses a mixed-method approach combining quantitative and qualitative methods to investigate social acceptance concerning drones. The study included 20 participants and comprised a virtual simulation, a team task, and a group discussion. This way, different aspects of drone acceptance were investigated. On the one hand, a simulative approach was tested to explore the visual perception of drone flights in an urban setting. On the other hand, the acceptance of various drone applications such as civil protection, parcel delivery, and air taxis was studied. Furthermore, this research identified requirements for coordinating and managing future drone traffic. This paper combines two acceptance models from the literature as a theoretical framework: an adoption of Chamata’s and Winterton’s Technical Acceptance Model by Krempel and the Unacceptability–Acceptance Scale by Hofinger.

AbstractObjectivesThe aims of this study were to explore the knowledge of EMS physicians (EMSPs) on the legal aspects related to the management of prehospital psychiatric emergencies, assess their degree of comfort, and measure their perceived communication skills.MethodsA survey was distributed to the 376 EMSPs working in the prehospital setting in Lombardy, Italy. Knowledge of medico-legal aspects was measured through multiple-choice questions; the Comfort Score (CS) and perceived Communication Skills Score (CSS) were calculated using Likert Scale questions. CS ranged from 11 points (lowest) to 44 (highest); CSS ranged from 8 points (lowest) to 32 (highest).ResultsA total of 272 EMSPs answered the survey (RR= 72.3%); 45.2% were women. Mean age was 45.76 years (SD 8.8). Mean percentage of correct knowledge test answers was 52.4 (SD 12.3); 81.3% believed to have little knowledge of the Italian legislation regarding this topic. Mean CS was 29.3 (SD 5.5); mean CSS was 19.1 (SD 3.9). A higher percentage of correct answers in the knowledge test was associated with lower CS (P =0.019). Higher self-appraised knowledge of the Italian legislation was associated with higher CS (P < 0.001) and higher CSS (P <0.001).ConclusionsThese findings should prompt policy makers to ensure proper training and support for EMSPs in the matter of prehospital psychiatric emergency management.

In recent years, civil drones have become increasingly present in the media and in everyday life. However, public acceptance of drones still seems limited, with acceptance rates usually ranging around 50%.

The COVID-19 pandemic resulted in an upsurge in the spread of diverse conspiracy theories (CTs) with real-life impact. However, the dynamics of user engagement remain under-researched. In the present study, we leverage Twitter data across 11 months in 2020 from the timelines of 109 CT posters and a comparison group (non-CT group) of equal size. Within this approach, we used word embeddings to distinguish non-CT content from CT-related content as well as analysed which element of CT content emerged in the pandemic. Subsequently, we applied time series analyses on the aggregate and individual level to investigate whether there is a difference between CT posters and non-CT posters in non-CT tweets as well as the temporal dynamics of CT tweets. In this regard, we provide a description of the aggregate and individual series, conducted a STL decomposition in trends, seasons, and errors, as well as an autocorrelation analysis, and applied generalised additive mixed models to analyse nonlinear trends and their differences across users. The narrative motifs, characterised by word embeddings, address pandemic-specific motifs alongside broader motifs and can be related to several psychological needs (epistemic, existential, or social). Overall, the comparison of the CT group and non-CT group showed a substantially higher level of overall COVID-19-related tweets in the non-CT group and higher level of random fluctuations. Focussing on conspiracy tweets, we found a slight positive trend but, more importantly, an increase in users in 2020. Moreover, the aggregate series of CT content revealed two breaks in 2020 and a significant albeit weak positive trend since June. On the individual level, the series showed strong differences in temporal dynamics and a high degree of randomness and day-specific sensitivity. The results stress the importance of Twitter as a means of communication during the pandemic and illustrate that these beliefs travel very fast and are quickly endorsed.

A crucial issue in the development of therapies to treat pathologies of the central nervous system is represented by the availability of non-invasive methods to study the three-dimensional morphology of spinal cord, with a resolution able to characterize its complex vascular and neuronal organization. X-ray phase contrast micro-tomography enables a high-quality, 3D visualization of both the vascular and neuronal network simultaneously without the need of contrast agents, destructive sample preparations or sectioning. Until now, high resolution investigations of the post-mortem spinal cord in murine models have mostly been performed in spinal cords removed from the spinal canal. We present here post-mortem phase contrast micro-tomography images reconstructed using advanced computational tools to obtain high-resolution and high-contrast 3D images of the fixed spinal cord without removing the bones and preserving the richness of micro-details available when measuring exposed spinal cords. We believe that it represents a significant step toward the in-vivo application.

Conservation of species is often focused either only on those that are endangered, or on maximising the number recorded on species lists. However, species share space and time with others, thus interacting and building frameworks of relationships that can be unravelled by community-level network analysis. It is these relationships that ultimately drive ecosystem function via the transfer of energy and nutrients. However interactions are rarely considered in conservation planning. Network analysis can be used to detect key species (“hubs”) that play an important role in cohesiveness of networks. We applied this approach to plant-pollinator communities on two montane Northern Apennine grasslands, paying special attention to the modules and the identity of hubs. We performed season-wide sampling and then focused the network analyses on time units consistent with plant phenology. After testing for significance of modules, only some modules were found to be significantly segregated from others. Thus, networks were organized around a structured core of modules with a set of companion species that were not organized into compartments. Using a network approach we obtained a list of important plant and pollinator species, including three Network Hubs of utmost importance, and other hubs of particular biogeographical interest. By having a lot of links and high partner diversity, hubs should convey stability to networks. Due to their role in the networks, taking into account such key species when considering the management of sites could help to preserve the greatest number of interactions and thus support many other species.

Conservation of species is often focused either only on those that are endangered, or on maximising the number recorded on species lists. However, species share space and time with others, thus interacting and building frameworks of relationships that can be unravelled by community-level network analysis. It is these relationships that ultimately drive ecosystem function via the transfer of energy and nutrients. However interactions are rarely considered in conservation planning. Network analysis can be used to detect key species (“hubs”) that play an important role in cohesiveness of networks. We applied this approach to plant-pollinator communities on two montane Northern Apennine grasslands, paying special attention to the modules and the identity of hubs. We performed season-wide sampling and then focused the network analyses on time units consistent with plant phenology. After testing for significance of modules, only some modules were found to be significantly segregated from others. Thus, networks were organized around a structured core of modules with a set of companion species that were not organized into compartments. Using a network approach we obtained a list of important plant and pollinator species, including three Network Hubs of utmost importance, and other hubs of particular biogeographical interest. By having a lot of links and high partner diversity, hubs should convey stability to networks. Due to their role in the networks, taking into account such key species when considering the management of sites could help to preserve the greatest number of interactions and thus support many other species.

Pigmented rice varieties are rich in antioxidant and anti-inflammatory compounds (e.g. anthocyanins and proanthocyanidins). Therefore, their consumption could exert beneficial effects, particularly in people suffering from chronic diseases (e.g., celiac disease). Pigmented rice is commonly consumed as brown rice, but technological treatments could be applied to reduce its cooking time and improve its nutritional value (vitamins and minerals). In this study, two relatively new pigmented varieties (Violet and Orange) were characterized in terms of phenolic content and antioxidant capacity and the impact of two technological treatments (e.g., milling and parboiling) on their phytochemical composition was evaluated. Two pigmented and one non-pigmented Italian varieties were included for comparison. Both technological processes affected the concentration of phenolic compounds and their relative antioxidant property. Although milling mainly reduced the phenolic content and the antioxidant activity, anthocyanins seem to be more affected by parboiling (reduction of 91.5%). Despite the effects of technological treatments on active compounds, pigmented varieties still represent an interesting antioxidant source when compared to the non-pigmented ones.

Stem cell engineering of striatal medium spiny neurons (MSNs) is a promising strategy to understand diseases affecting the striatum and for cell-replacement therapies in different neurological diseases. Protocols to generate cells from human pluripotent stem cells (PSCs) are scarce and how well they recapitulate the endogenous fetal cells remains poorly understood. We have developed a protocol that modulates cell seeding density and exposure to specific morphogens that generates authentic and functional D1- and D2-MSNs with a high degree of reproducibility in 25 days of differentiation. Single-cell RNA sequencing (scRNA-seq) shows that our cells can mimic the cell-fate acquisition steps observed in vivoin terms of cell type composition, gene expression, and signaling pathways. Finally, by modulating the midkine pathway we show that we can increase the yield of MSNs. We expect that this protocol will help decode pathogenesis factors in striatal diseases and eventually facilitate cell-replacement therapies for Huntington’s disease (HD).

Mechanical micromachining is a very flexible and widely exploited process, but its knowledge should still be improved since several incompletely explained phenomena affect the microscale chip removal. Several models have been developed to describe the machining process, but only some of them consider a rounded edge tool, which is a typical condition in micromachining. Among these models, the Waldorf's slip-line field model for the macroscale allows to separately evaluate shearing and ploughing force components in orthogonal cutting conditions; therefore, it is suitable to predict cutting forces when a large ploughing action occurs, as in micromachining. This study aims at demonstrating how this model is suitable also for micromachining conditions. To achieve this goal, a clear and repeatable procedure has been developed for objectively validating its force prediction performance at low uncut chip thickness (less than 50 μm) and relatively higher cutting edge radius. The proposed procedure makes the model generally applicable after a suitable and non-extensive calibration campaign. This article shows how calibration experiments can be selected among the available cutting trial database based on the model force prediction capability. Final validation experiments have been used to show how the model is robust to a cutting speed variation even if the cutting speed is not among the model quantities. A suitable set-up, especially designed for microturning conditions, has been used to measure forces and chip thickness. Tests have been performed on 6082-T6 Aluminum alloy with different cutting speeds and different ratios between uncut chip thickness and cutting edge radius.

Objective To evaluate the prevalence of fistulas after palate repair and analyze their location and association with possible causal factors.Design Retrospective analysis of patient records and evaluation of preoperative initial photographs.Setting Tertiary craniofacial center.Participants Five hundred eighty-nine individuals with complete unilateral cleft lip and palate that underwent palate repair at the age of 12 to 36 months by the von Langenbeck technique, in a single stage, by the plastic surgery team of the hospital, from January 2003 to July 2007.Interventions The cleft width was visually classified by a single examiner as narrow, regular, or wide. The following regions of the palate were considered for the location: anterior, medium, transition (between hard and soft palate), and soft palate.Main outcome measures Descriptive statistics and analysis of association between the occurrence of fistula and the different parameters were evaluated.Results Palatal fistulas were observed in 27% of the sample, with a greater proportion at the anterior region (37.11%). The chi-square statistical test revealed statistically significant association (P= .05) between the fistulas and initial cleft width (P= .0003), intraoperative problems (P= .0037), and postoperative problems (P= .00002).Conclusions The prevalence of palatal fistula was similar to mean values reported in the literature. Analysis of causal factors showed a positive association between palatal fistulas with wide and regular initial cleft width and intraoperative and postoperative problems. The anterior region presented the greatest occurrence of fistulas.

Mechanical micromachining is a very flexible and widely exploited process, but its knowledge should still be improved since several typical phenomena play a role on the microscale chip removal (e.g. “minimum chip thickness effect”, microstructure influence on cutting forces, stable built-up edge, etc.). Several models have been developed to describe the machining process, but only some of them take into account a rounded-edge tool, which is a typical condition in micromachining. Among these models, the slip-line field model developed by Waldorf for the macroscale allows to separately evaluate shearing and ploughing force components in orthogonal cutting conditions, therefore it is suitable to predict the cutting forces when a large ploughing action occurs, as in micromachining. The present work aims at objectively verifying the cutting and feed force prediction performance of the Waldorf model within typical microscale cutting conditions (uncut chip thickness lower than 50μm and comparable in size to cutting edge radius) in its original version and in a modified version considering the partial effective rake angle. A suitable set-up, especially designed for microturning conditions, has been used in this research to measure forces and chip thickness. Tests have been carried out on C38500 brass (CuZn39Pb3) with different cutting speeds and different ratios between uncut chip thickness and cutting edge radius.

The pathophysiology of Alzheimers disease (AD) is influenced by sorting-protein related receptor (sorLa) that is less expressed in AD patients. The gene encoding sorLa (SORL1) has been investigated as a susceptibility factor for late-onset AD (LOAD) with conflicting results. Our objectives were to confirm the association between SORL1 SNPs and LOAD in two independent South-European centers and to perform a mega-analysis of published samples. We analyzed three SORL1 SNPs (intron 6: rs668387; rs689021; rs641120) from the Greece-Italy Genetic Association Study on lateonset AD (GIGAS_LOAD). Greek sample included 96 patients with LOAD (DSM-IV) and 120 unrelated controls. In Italy, a community-based sample is ongoing. 47 LOAD patients and 165 controls were recruited until study endpoint. These samples and previously published ones (Alzgene) were pooled as in a single study. A test for trend was used to analyze genotype association. In the GIGAS_LOAD sample no association was detected between SORL1 genotypes and LOAD. Conversely all SNPs were associated with LOAD in mega-analysis based on ordinal classification of genotypes (Armitages test: p<0.001). Although our analysis of pooled samples has positive results for the association between SORL1 and AD, there is substantial heterogeneity across studies. Thus further examination into SORL1 SNPs and the population is necessary to determine the role of SORL1 in LOAD.

Advanced Surface Movement Guidance and Control Systems (A-SMGCS) comprise a range of new technologies for both the flight deck and ground air traffic control and is supposed to enable increased safety and a more efficient throughput at presently highly congested major airports. A flight deck A-SMGCS module is the onboard guidance system TARMAC-AS. This module consists of controller pilot data link communication (CPDLC) and an electronic moving map (EMM), which also serves as a cockpit display of traffic information (CDTI). TARMAC-AS is evaluated in an investigation involving 49 commercial pilots who performed a series of approach, landing and taxiing simulation trials under varied visibility, which were completed in a fixed-base cockpit simulator. Results support the notion that an EMM + CPDLC + CDTI improve the effectiveness of taxi navigation. A potential negative impact of observed increased head-down times to the compelling TARMAC display on unexpected outside scene obstacle detection was not substantiated.

A pulse liquid chromatographic technique, set up by using an HPLC apparatus, is proposed for the characterization of the surface acidity of solid acids in liquids in view of their use as catalysts in liquid–solid reactions. Dosed amounts of solutions of two different basic probe molecules (2-phenylethylamine, pKa= 9.84 and aniline, pKa= 4.63) in solvents of different polarity were injected onto solid samples (acidic resins, Amberlites and a mixed silica–zirconia oxide) at a constant solvent flow rate. Titration with the weakest basic probe (aniline) rather than 2-phenylethylamine generally led to a lower amount of acidic sites. The amount of acidic sites determined at the surface was greatly affected by the solvent. With silica–zirconia, a higher amount of acidic sites was established by titration in apolar rather than polar solvents. The results have been compared with those obtained by conventional methods, i.e. ion exchange employing salts and, for silica–zirconia, Hammett titrations. The quite satisfactory results obtained are of some importance in establishing valuable relationships between catalyst acidity and catalytic activity in various solvents.

The acidity properties of niobic acid (Nb2O5·nH2O) and niobium phosphate (NbOPO4) surfaces have been studied by different methods and under various conditions in order to obtain comprehensive information about the number, strength, and strength distribution of their acid sites. Four base probe molecules of different basicities (ammonia, pyridine, 2-phenylethylamine, and aniline) have been used. Gas−solid and liquid−solid titrations by these basic probes have been performed, using static or dynamic adsorption (calorimetry linked to volumetry, reaction calorimetry, pulse flow volumetry using a HPLC apparatus) and desorption (thermogravimetric analysis) techniques. The intrinsic surface acidities of the two solids, determined under vacuum or inert atmospheres, were compared with the effective acidities measured in liquids of different polarities and proticities (decane, cycloexane, toluene, methanol, and isopropanol). The effective acidities of the two surfaces could be related to their catalytic activities measured in the fructose dehydration reaction in aqueous phase. The superior catalytic activity of niobium phosphate compared to niobic acid is related to its higher number of surface sites maintaining acid characteristics even in highly polar and protic solvents.

Caveolins are the main structural proteins of glycolipid/cholesterol-rich plasmalemmal invaginations, termed caveolae. In addition, caveolin-1 isoform takes part in membrane remodelling as it binds and transports newly synthesized cholesterol from endoplasmic reticulum to the plasma membrane. Caveolin-1 is expressed in many cell types, including hippocampal neurons, where an abundant SNAP25-caveolin-1 complex is detected after induction of persistent synaptic potentiation. To ascertain whether caveolin-1 influences neuronal voltage-gated Ca2+ channel basal activity, we stably expressed caveolin-1 into transfected neuroblastoma×glioma NG108-15 hybrid cells [cav1(+) clone] that lack endogenous caveolins but express N-type Ca2+ channels upon cAMP-induced neuronal differentiation. Whole-cell patch-clamp recordings of cav1(+) cells demonstrated that N-type current density was reduced in size by ∼70% without any significant change in the time course of activation and inactivation and voltage dependence. Moreover, the cav1(+) clone exhibited a significantly increased proportion of membrane cholesterol compared to wild-type NG108-15 cells. To gain insight into the mechanism underlying caveolin-1 lowering of N-current density, and more precisely to test whether this was indirectly caused by caveolin-1-induced enhancement of membrane cholesterol, we compared single N-type channel activities in cav1(+) clone and wild-type NG108-15 cells enriched with cholesterol after exposure to a methyl-β-cyclodextrin-cholesterol complex. A lower Ca2+ channel activity was recorded from cell-attached patches of both cell types, thus supporting the view that the increased proportion of membrane cholesterol is ultimately responsible for the effect. This is due to a reduction in the probability of channel opening caused by a significant decrease of channel mean open time and by an increase of the frequency of null sweeps.

N-Methyl-D-aspartic acid (NMDA) is an agonist at the homonymous receptor implicated in the development of neuronal sensitization and its behavioral correlates. An effective modulation of the NMDA effects, achieved also by uncompetitive antagonists, could contribute to controlling pain symptoms in several neuropathic syndroms. Because nefopam is a known analgesic derivative of orphenadrine and of its congener diphenhydramine, both uncompetitive NMDA receptor antagonists, we tested the effect of nefopam on the developing pain and neuronal anomalies in an animal model of chronic pain with NMDA receptor involvement. A single intraperitoneal injection of nefopam was administered twenty minutes prior to the chronic constriction injury of the sciatic nerve (CCI rats). In the first 10 days, nefopam (30 mg/kg) significantly decreased behavioral signs of neuropathic pain and the stimulus-evoked electrophysiological anomalies in recordings at 14 days, with only slight manifestation afterwards. The dose of 20 mg/kg was ineffective. Nefopam injected after constriction was ineffective. In normal non-operated rats, Nefopam had no effect on the electrophysiological and behavioral parameters. Iontophoretic nefopam (1 mM, 50–80 nA, positive current) in normal rats did not change the spontaneous neuronal activity, but reduced the mean response to noxious stimuli and the concurrent iontophoretic NMDA evoked activity. In CCI rats, iontophoretic nefopam did not significantly modify the spontaneous hyperactivity but reduced significantly both the frequency of the responses to noxious stimuli, and the duration of the afterdischarge. We propose that nefopam exerts a preventive analgesic effect, with a possible role in modulating NMDA receptor-mediated effects in central sensitization.

Programmed cell death (PCD) is an essential part of the defence response in plants and animals against pathogens. Here, we report that PCD is also involved in defence against pathogens of fungi. Vegetative incompatibility is a self/non–self recognition system in fungi that results in PCD when cells of incompatible strains fuse. We quantified the frequency of cell death associated with six vegetative incompatibility (vic) genes in the filamentous ascomycete fungus Cryphonectria parasitica. Cell death frequencies were compared with the effects of vicgenes on transmission of viruses between the same strains. We found a significant negative correlation between cell death and virus transmission. We also show that asymmetry in cell death correlates with asymmetry in virus transmission; greater transmission occurs into vicgenotypes that exhibit delayed or infrequent PCD after fusion with an incompatible strain. Furthermore, we found that virus infection can have a significant, strain–specific, positive or negative effect on PCD. Specific interactions between vicgene function and viruses, along with correlations between cell death and transmission, strongly implicate PCD as a host–mediated pathogen defence strategy in fungi.

Summary.:  Nefopam hyghochloride is a potent analgesic compound commercialized in most Western Europe for 20 years, which possesses a profile distinct from that of opioids or anti-inflammatory drugs. Previous evidence suggested a central action of nefopam but the detailed mechanisms remain unclear. While, nefopam structure resembles that of orphenadrine, an uncompetitive NMDA receptor antagonist, here we report that differently from orphenadrine, nefopam (100 μM) failed to protect cultured cerebellar neurons from excitotoxicity following direct exposure of neurons to glutamate. Moreover, nefopam failed to displace MK-801 binding to hippocampal membranes. Nefopam effectively prevented NMDA receptor-mediated early appearance (30 min) of toxicity signs induced by the voltage sensitive sodium channel (VSSC) activator veratridine. The later phase (24 h) of neurotoxicity by veratridine occurring independently from NMDA receptor activation, was also prevented by nefopam. Nefopam effect was not mimicked by the GABA receptor agonist muscimol.

Associative fiber systems in the entorhinal cortex (EC) have been extensively studied in different mammals with tracing techniques. The largest contingent of intra-EC cortico-cortical fibers runs in the superficial layers and is distributed predominantly within longitudinal cortical bands. We studied the patterns of intrinsic EC connectivity in the in vitro isolated guinea pig brain preparation by performing current-source density analysis of field potential laminar profiles recorded with multi-channel silicon probes. The response pattern evoked by stimulation of the lateral olfactory tract was utilized to identify the lateral (l-EC) and medial (m-EC) entorhinal cortex. Stimulation of the deep layers did not evoke consistent responses. Local stimulation of the superficial layers in different portions of the EC induced an early, possibly direct response restricted to layer II–III in the close proximity to the stimulating electrode, followed by a late potential in the superficial layer I, that propagated at distance with a progressively increasing latency. The monosynaptic nature of the delayed response was verified by applying a pairing test. The results demonstrated that stimulation in the rostral-medial part of the EC generated activity restricted to the rostral pole of the l-EC, stimulation of the m-EC induced an associative activation that propagated rostrocaudally within the m-EC, stimulation of the caudal pole of the m-EC induced an additional response directed laterally, and stimulation of the lateral band of the EC determined a prominent longitudinal propagation of neuronal activity, but also induced associative potentials that propagated medially. The results are in partial agreement with the general picture derived from the anatomical studies performed in different species. Even though the largest associative interactions between superficial layers are restricted within either the m-EC or the l-EC, both rostral and caudal stimuli in the EC region close to the rhinal sulcus induced activity that propagated across the border between l- and m-EC.

In anaesthetised and paralysed rats with chronic constriction of the sciatic nerve, the effects of subcutaneous contralateral lidocaine (100 μl) on the activity of lumbar (L4–L5) wide dynamic range neurons ipsilateral to the constriction have been investigated. The results show reduction of the spontaneous hyperactivity for 60 min; suppression or reduction of the responses to contralateral noxious stimulation for 60 min; lack of effect on the responses to ipsilateral noxious stimulation, except for the afterdischarge duration, reduced for 60 min. The finding that the altered neuronal activity following peripheral nerve injury associated to behavioural signs of neuropathic pain, can be reduced by contralateral treatment, may provide further suggestions to neuropathic pain mechanisms and management.

Acupuncture is largely used for pain control in several pathological conditions. Its effects on the central nervous system are not well defined. We investigated the effect of the application of acupuncture to 13 normal subjects (males, 21–32 years). H215O bolus PET scans were read before the application of the needles (Rest, R) and after 25 min of needle insertion. Data were acquired by scanning in 3-D mode. The acupuncture application, true acupuncture (TA), was alternated to a placebo needle application (PA) in two different sequences (seven and six subjects, respectively), either R,PA,R, TA or R,TA,R,PA, a period of 15 min being left after every first TA or PA to allow for the recovery of basal conditions. Here we show that classic acupuncture activates the left Anterior Cingulus, the Insulae bilaterally, the Cerebellum bilaterally, the left Superior Frontal Gyrus, and the right Medial and Inferior Frontal Gyri. Most of the activated areas are shared with areas activated in acute and chronic pain states as described in the literature. Thus acupuncture appears to act by activating areas also involved in pain. This indicates that acupuncture could relief pain by unbalancing the equilibrium of distributed pain-related central networks.

The perirhinal cortex is a key structure involved in memory consolidation and retrieval. In spite of the extensive anatomical studies that describe the intrinsic and extrinsic associative connections of the perirhinal cortex, the activity generated within such a network has been poorly investigated. We describe here the pattern of synaptic interactions that subtend the responses evoked in area 36 of the perirhinal cortex by neocortical and local stimulation. The experiments were carried out in the in vitro isolated guinea pig brain. The synaptic perirhinal circuit was reconstructed by integrating results obtained during intracellular recordings from layer II–III neurons with simultaneous current source density analysis of laminar profiles performed with 16-channel silicon probes. Both neocortical and local stimulation of area 36 determined a brief monosynaptic excitatory potential in layer II–III neurons, followed by a biphasic synaptic inhibitory potential possibly mediated by a feed-forward inhibitory circuit at sites close to the stimulation electrode and a late excitatory postsynaptic potential (EPSP) that propagated at distance within area 36 along the rhinal sulcus. During a paired-pulse stimulation test, the inhibitory postsynaptic potential (IPSP) and the late EPSP were abolished in the second conditioned response, suggesting that they are generated by poli-synaptic circuits. Current source density analysis of the field responses demonstrated that 1) the monosynaptic activity was generated in layers II–III and 2) the sink associated to the disynaptic responses was localized within the superficial layer of area 36. We conclude that the neocortical input induces a brief monosynaptic excitation in area 36 of the perirhinal cortex, that is curtailed by a prominent inhibition and generates a recurrent excitatory associative response that travels at distance within area 36 itself. The results suggest that the perirhinal cortex network has the potentials to integrate multimodal incoming neocortical information on its way to the hippocampus.

Cross‐Cultural Filmmaking: A Handbook for Making Documentary and Ethnographic Films and Videos. Ilisa Barbash and Lucien Taylor. Berkeley, Los Angeles, London: University of California Press, 1997. Name and subject indexes, appendices, filmography; 557 pp. $24.95, paper.