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Permeability is a key rock property important for scientific applications that require simulation of fluid flow. Although permeability is determined using core flooding experiments, recent advancements in micro-CT imaging and pore scale fluid flow simulations have made it possible to constrain permeability honoring pore scale rock structure. Previous studies have reported that complex association of pores and solid grains often results in preferential flow paths which influence the resulting velocity field and, hence, the upscaled permeability value. Additionally, the pore structure may change due to geochemical processes such as microbial growth, mineral precipitation and dissolution. This could result in a flow field which dynamically evolves spatially and temporally. It would require numerous experiments or full physics simulations to determine the resultant upscaled Darcy permeability for such dynamically changing systems. This study presents a graph theory-based approach to upscale permeability from pore-to-Darcy scale for changing pore structure. The method involves transforming a given micro-CT rock image to a graph network map followed by the identification of the least resistance path between the inlet and the outlet faces using Dijkstra's algorithm where resistance is quantified as a function of pore sizes. The least resistance path is equivalent to the path of lowest resistance within the domain. The method was tested on micro-CT images of the samples of Sherwood Sandstone, Ketton Limestone and Estaillades Limestone. The three micro-CT images were used to generate 30 synthetic scenarios for geochemically induced pore structure changes covering a range of pore and solid grain growth. The least resistance value obtained from Dijkstra's algorithm was observed to correlate with upscaled permeability value determined from full physics simulations, while improving computational efficiency by a factor of 250. This provides confidence in using graph theory method as a proxy for full physics simulations for determining effective permeability for samples with changing pore structure.

Transforming growth factor- (TGF-) induces epithelial-mesenchymal transition (EMT) of epithelial cells in both normal embryonic development and certain pathological contexts. Here, we show that TGF- induced-EMT in human lung cancer cells (A549; adenocarcinoma cells) mediates tumor cell migration and invasion phenotypes. To gain insights into molecular events during EMT, we employed a global stable isotope labeled profiling strategy using iTRAQ reagents, followed by 2DLC−MS/MS, which identified a total of 51 differentially expressed proteins during EMT; 29 proteins were up-regulated and 22 proteins were down-regulated. Down-regulated proteins were predominantly enzymes involved in regulating nutrient or drug metabolism. The majority of the TGF--induced proteins (such as tropomyosins, filamin A, B, & C, integrin-1, heat shock protein27, transglutaminase2, cofilin, 14-3-3 zeta, ezrin-radixin-moesin) are involved in the regulation of cell migration, adhesion and invasion, suggesting the acquisition of a invasive phenotype.

Calibration of patient dose monitoring devices in diagnostic radiology has become increasingly important in the light of new legislation that requires monitoring of patient dose against local and national diagnostic reference levels. An investigation was conducted into the long-term stability of 41 dose-area product (DAP) meters over a period of approximately 5 years, to assess the suitability of an annual calibration regimen. For DAP meters fitted to overcouch X-ray tubes, 77% of calibrations were within 10%, whilst for undercouch tubes only 50% of calibrations were within 10%. These findings suggest that annual calibration may be too infrequent. Suitable calibration frequencies for different clinical workloads are discussed.