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Nunes, JP, Blazevich, AJ, Schoenfeld, BJ, Kassiano, W, Costa, BDV, Ribeiro, AS, Nakamura, M, Nosaka, K, and Cyrino, ES. Determining changes in muscle size and architecture after exercise training: One site does not fit all. J Strength Cond Res38(4): 787–790, 2024—Different methods can be used to assess muscle hypertrophy, but the effects of training on regional changes in muscle size can be detected only using direct muscle measurements such as muscle thickness, cross-sectional area, or volume. Importantly, muscle size increases vary across regions within and between muscles after resistance training programs (i.e., heterogeneous, or nonuniform, muscle hypertrophy). Muscle architectural changes, including fascicle length and pennation angle, after resistance and stretch training programs are also region-specific. In this paper, we show that the literature indicates that a single-site measure of muscle shape does not properly capture the effects achieved after exercise training interventions and that conclusions concerning the magnitude of muscle adaptations can vary substantially depending on the muscle site to be examined. Thus, we propose that measurements of muscle size and architecture should be completed at multiple sites across regions between the agonist muscles within a muscle group and along the length of the muscles to provide an adequate picture of training effects.

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Kassiano, W, Costa, B, Kunevaliki, G, Soares, D, Stavinski, N, Francsuel, J, Carneiro, MAS, Tricoli, I, Nunes, JP, Ribeiro, AS, and Cyrino, ES. Muscle swelling of the triceps surae in response to straight-leg and bent-leg calf raise exercises in young women. J Strength Cond Res37(7): e438–e443, 2023—Triceps surae muscle swelling in response to different resistance exercises remains to be determined. This study compared the effects of straight-leg (STRA-leg) calf raise vs. bent-leg (BENT-leg) calf raise exercises on triceps surae muscle swelling. Seventeen young women (23.7 ± 4.0 years; 67.4 ± 16.0 kg; and 163.5 ± 7.2 cm) performed 2 resistance training sessions; in one, they performed the STRA-leg calf raise, and in another, they performed the BENT-leg calf raise. A randomized, cross-over, and counterbalanced design was adopted for this investigation. The subjects performed 4 sets of 20 repetitions maximum until concentric muscular failure. The muscle thickness of the gastrocnemius medial (GM), gastrocnemius lateral (GL), and soleus (SOL) was taken through B-mode ultrasound before and immediately after the calf raise exercises. STRA-leg calf raise elicited greater increases in muscle thickness of GM (+8.8% vs. −0.9%, p< 0.001) and GL (+14.5% vs. +7.0%, p< 0.001) than BENT-leg calf raise. Conversely, BENT-leg calf raise elicited greater increases in SOL muscle thickness than STRA-leg calf raise (+15.4% vs. +7.7%, p< 0.001). From a practical perspective, the STRA-leg calf raise should be preferred if the aim is stimulating the 3 muscles that comprise the triceps surae, whereas the BENT-leg calf raise should be prioritized if the target muscle is the SOL.

Kassiano, W, Costa, B, Nunes, JP, Ribeiro, AS, Schoenfeld, BJ, and Cyrino, ES. Which ROMs lead to Rome? a systematic review of the effects of range of motion on muscle hypertrophy. J Strength CondRes 37(5): 1135–1144, 2022—Resistance exercise range of motion (ROM) influences muscular adaptations. However, there are no consistent practical guidelines about the optimal ROM for maximizing muscle hypertrophy. The objective of this article was to systematically review the literature for studies that compared the effects of full ROM (fROM) and partial ROM (pROM) on muscle hypertrophy. PubMed/MEDLINE, Scopus, and Web of Science databases were searched to identify articles from the earliest record up to and including April 2022. We calculated the effect size (ES) scores of the variables of interest. Eleven studies were included in the review. Full ROM and pROM performed in the initial part of the ROM elicited greater muscle hypertrophy of the rectus femoris, vastus lateralis, biceps brachii, and brachialis distal sites (between-groups ES: 0.20–0.90) than pROM performed in the final part of the ROM. fROM elicited greater muscle growth on the gluteus maximus and adductors than pROM in the final part of the ROM (between-groups ES: 0.24–0.25). Initial pROM produced more favorable proximal rectus femoris hypertrophy than fROM (between-groups ES: 0.35–0.38). pROM in the middle part of the ROM elicited greater triceps brachii hypertrophy than fROM (between-group ES: 1.21). In conclusion, evidence suggests that when training at a longer muscle length—through either pROM or fROM—some muscles, such as quadriceps femoris, biceps brachii, and triceps brachii, tend to experience optimal growth. Thus, the use pROM in the initial part of the excursion in combination with fROM training should be considered when prescribing hypertrophy-oriented resistance training programs.

Aims: The concomitant increase in skeletal muscle mass (SMM) and decrease in fat mass has been termed body recomposition. This study aimed to analyze the influence of pre-training levels of fat mass on body recomposition, muscular strength, and (phase angle) PhA after 24 weeks of resistance training (RT) in older women. Methods: Data from 99 older women (68.6 ± 5.7 years, 65.7 ± 8.6 kg, 155.1 ± 5.8 cm, 27.2 ± 3.1 kg/m2) was retrospectively analyzed. Participants were separated into tertiles according to the amount of fat mass at baseline as follows: low fat mass (L-FM, n= 33), moderate fat mass (M-FM, n= 33), and high fat mass (H-FM, n= 33). The participants underwent a RT program consisting of eight exercises, three sets per exercise, with a load between 8 and 15 RM, performed three times per week for 24 weeks. The SMM and fat mass were evaluated by dual-energy X-ray absorptiometry (DXA). Body recomposition was determined by the composite Z-score of changes in SMM and fat mass. One repetition maximum (1RM) tests in chest press, knee extension, and preacher curl were assessed to verify muscular strength. Bioimpedance was used to determine phase angle. Results: Results indicated that after the RT period, a greater positive body recomposition was observed in the L-FM group than in M-FM and H-FM groups. Moreover, all groups increased muscular strength and phase angle with no significant difference among groups (P> 0.05). Conclusion: The present study results suggest that the initial amount of fat mass influences the body recomposition induced by RT in older women, with those with lower pre-training fat mass levels presenting higher levels of body recomposition. However, improvements in muscular strength and phase angle are not dependent on the amount of initial fat mass in older women.

Background: Bioelectrical impedance analysis (BIA) is a technique widely used for estimating body composition and health-related parameters. The technology is relatively simple, quick, and non-invasive, and is currently used globally in diverse settings, including private clinicians’ offices, sports and health clubs, and hospitals, and across a spectrum of age, body weight, and disease states. BIA parameters can be used to estimate body composition (fat, fat-free mass, total-body water and its compartments). Moreover, raw measurements including resistance, reactance, phase angle, and impedance vector length can also be used to track health-related markers, including hydration and malnutrition, and disease-prognostic, athletic and general health status. Body composition shows profound variability in association with age, sex, race and ethnicity, geographic ancestry, lifestyle, and health status. To advance understanding of this variability, we propose to develop a large and diverse multi-country dataset of BIA raw measures and derived body components. The aim of this paper is to describe the ‘BIA International Database’ project and encourage researchers to join the consortium. Methods: The Exercise and Health Laboratory of the Faculty of Human Kinetics, University of Lisbon has agreed to host the database using an online portal. At present, the database contains 277,922 measures from individuals ranging from 11 months to 102 years, along with additional data on these participants. Conclusion: The BIA International Database represents a key resource for research on body composition.

Nunes, JP, Marcori, AJ, Ribeiro, AS, Cunha, PM, Kassiano, W, Costa, BDV, Aguiar, AF, Nakamura, M, Mayhew, JL, and Cyrino, ES. Differential responsiveness for strength gain between limbs after resistance training in older women: Impact on interlimb asymmetry reduction. J Strength Cond Res36(11): 3209–3216, 2022—The present study compared strength responses between preferred (PREF) and nonpreferred (N-PREF) legs in older women. Muscular strength was measured unilaterally using an isokinetic dynamometer and was analyzed for reproducibility scores, acute performance, and responsiveness to a resistance training (RT) program. One hundred eleven women (aged ≥60 years) performed 12 weeks of whole-body RT (3 times a week; 4 lower-body exercises). Reproducibility scores (intraclass correlation coefficient ≥0.920; following test-retests in part of the sample at pretraining; n= 19), average acute performances, and average strength gains (PREF = ∼6.9%; N-PREF = ∼7.2%) were similar between legs (p> 0.05). However, the individual analyses showed that some subjects were considered responders to strength gains in 1 leg, whereas nonresponders in the other. Nonetheless, when considering the responses in all strength tests, most subjects (91%) were considered responder to at least 1 measure. In addition, it was observed that the strength ratio between PREF/N-PREF legs was altered for those who presented some asymmetry at baseline, as the limbs became more symmetrical in all strength measures after the RT. In conclusion, we observed that some older women may have different levels of strength between legs; however, a traditional 12-week RT program with bilateral exercises can reduce such asymmetry by inducing greater strength gains in the weaker leg.

Supplemental Digital Content is Available in the Text.Kassiano, W, Nunes, JP, Costa, B, Ribeiro, AS, Schoenfeld, BJ, and Cyrino, ES. Does varying resistance exercises promote superior muscle hypertrophy and strength gains? A systematic review. J Strength Cond Res36(6): 1753–1762, 2022—Fitness professionals routinely employ a variety of resistance training exercises in program design as a strategy to enhance muscular adaptations. However, it remains uncertain whether such an approach offers advantages over a fixed-exercise selection. The objective of this review was to review the effects of exercise variation on muscle hypertrophy and strength. A search of the literature was conducted using PubMed/MEDLINE, Scopus, and Web of Science databases. Eight studies were identified as meeting inclusion criteria. The combined total sample of the studies was N= 241, comprising all young men. The methodological quality of included studies was considered “good” and “excellent” based on the Physiotherapy Evidence Database Scale. The available studies indicate that varying exercise selection can influence muscle hypertrophy and strength gains. Some degree of systematic variation seems to enhance regional hypertrophic adaptations and maximize dynamic strength, whereas excessive, random variation may compromise muscular gains. We conclude that exercise variation should be approached systematically with a focus on applied anatomical and biomechanical constructs; on the contrary, employing different exercises that provide a redundant stimulus, as well as excessive rotation of different exercises (i.e., high frequency of change), may actually hinder muscular adaptations.

Carneiro, MAS, de Oliveira Júnior, GN, de Sousa, JFR, Murta, EFC, Orsatti, CL, Michelin, MA, Cyrino, ES, and Orsatti, FL. Effects of resistance training at different loads on inflammatory biomarkers, muscle mass, muscular strength, and physical performance in postmenopausal women. J Strength Cond Res36(6): 1582–1590, 2022—It has been suggested that the effect of resistance training (RT) on circulating proinflammatory biomarkers may be dependent on muscle mass gain. A few recent studies have suggested that lower-load RT (LLRT; loads <50% of 1 repetition maximum [1RM] and repetition performed until, or close to, voluntary concentric failure) may be superior to higher-load RT (HLRT; loads >70% of 1RM) in increasing muscle mass. Hence, this study aimed to test whether LLRT is superior to HLRT for increasing muscle mass (total fat-free mass [TFFM] and leg fat-free mass [LFFM]) and improving circulating inflammatory biomarkers (interleukin [IL]-6, IL1-ra, tumor necrosis factor [TNF]-α, and extracellular heat shock protein [eHSP]70) in postmenopausal women (PW) (primary outcome). The secondary outcome was to compare the changes in muscular strength and physical performance (4-meter walking test [4-M], timed-up-and-go [TUG] test, and sit-to-stand [STS] test) between the LLRT and HLRT. The PW were randomized into 2 groups: LLRT (n= 14; loads necessary to perform 30–35 repetitions) and HLRT (n= 15; loads necessary to perform 8–12 repetitions). The greater magnitude of increase in LFFM (p= 0.033) was observed in LLRT when compared with HLRT. Moreover, there was a trend for a greater increase in TFFM in LLRT over HLRT (p= 0.070). However, there were similar improvements in TNF-α and muscular strength (p< 0.001). Furthermore, there was no significant difference between the RT schemes on IL-6, IL-1ra, and eHSP70 levels. Thus, although performing LLRT until, or close to, voluntary concentric failure seems to provide a greater stimulus for an increase in muscle mass than HLRT, it does not seem to affect the responses in circulating inflammatory biomarkers, muscular strength, and physical performance in PW.

Amarante do Nascimento, M, Nunes, JPA, Pina, FLC, Ribeiro, AS, Carneiro, NH, Venturini, D, Barbosa, DS, Mayhew, JL, and Cyrino, ES. Comparison of 2 weekly frequencies of resistance training on muscular strength, body composition, and metabolic biomarkers in resistance-trained older women: Effects of detraining and retraining. J Strength Cond Res36(5): 1437–1444, 2022—This study aimed to compare the effects of 2 weekly frequencies of resistance training (RT) on muscular strength, body composition, and metabolic biomarkers in previously resistance-trained older women after detraining and retraining. Forty subjects (>60 years) performed RT (8 exercises, 1 set of 10–15 repetitions maximum) 2 (G2x) or 3 (G3x) times per week over 12 weeks of training and retraining. After training, subjects were detrained for 12 weeks. After detraining, there were significant decreases (p< 0.05) in upper-body (∼12%) and lower-body (∼14%) muscular strength, fat-free mass (FFM) (∼2%), and testosterone (∼26%), whereas increases were revealed for fat mass (FM) (∼4%), relative body fat (∼3%), fasting glucose (∼8%), low-density lipoprotein cholesterol (LDL-C) (∼21%), and triglycerides (∼24%), with no differences between groups (p> 0.05). Following retraining, there were significant increases (p< 0.05) for upper (∼7%) and lower (∼10%) muscular strength, FFM (∼2%), and testosterone (∼20%). In contrast, decreases were found for FM (∼7%), relative body fat (∼3%), fasting glucose (∼6%), LDL-C (∼14%), and triglycerides (∼21%), also with no differences between groups (p> 0.05). Gains after retraining were lower than after training (p< 0.05) only for upper- and lower-body muscular strength (∼6%) and testosterone (∼11%). Total cholesterol, high-density lipoprotein cholesterol, IGF-1, and C-reactive protein did not change at any point in the study for either group (p> 0.05). Our results suggest that older women can regain previous RT program benefits following detraining, regardless of the weekly training frequency. However, some fitness components may take longer to reestablish than the initial training level.

Ribeiro, AS, Picoloto, A, Nunes, JP, Bezerra, ES, Schoenfeld, BJ, and Cyrino, ES. Effects of different resistance training loads on the muscle quality index in older women. J Strength Cond Res36(5): 1445–1449, 2022—The purpose of this study was to investigate the effects of resistance training (RT) performed using low vs. moderate loads on muscular strength, muscle mass, and muscle quality index (MQI) in older women. Twenty-seven older women (71.5 ± 5 years, 65.2 ± 11 kg, 154.2 ± 5 cm, and 27.4 ± 4 kg·m−2) were randomized into 2 groups: one that performed RT with a low load (LOW, n= 13) and another that performed RT with a moderate load (MOD, n= 14). An 8-week RT program was performed 3 d·wk−1. Outcomes included 1-repetition maximum (1RM) tests and assessment of body composition by dual-energy X-ray absorptiometry. The LOW load consisted of a relative load at 15RM, whereas the MOD load consisted of a relative load at 10RM. Both groups presented significant changes from pre-training to post-training for chest press 1RM (LOW = 10.3%, and MOD = 11.1%), knee extension 1RM (LOW = 14.4%, and MOD = 16.6%), preacher curl 1RM (LOW = 12.4%, and MOD = 12.0%), fat-free mass (LOW = 2.1%, and MOD = 1.9%), body fat (LOW = −3.2%, and MOD = −2.7%), and MQI (LOW = 10.6%, and MOD = 11.3%). Results were similar (p> 0.05) between groups. These findings suggest that LOW and MOD loads are equally effective for promoting improvements in muscular strength and muscle growth as well as the MQI in older women.

Cunha, PM, Ribeiro, AS, Padilha, C, Nunes, JP, Schoenfeld, BJ, Cyrino, LT, Tomeleri, CM, Nascimento, MA, Antunes, M, Fernandes, RR, Barbosa, DS, Venturini, D, Burini, RC, Sardinha, LB, and Cyrino, ES. Improvement of oxidative stress in older women is dependent on resistance training volume: Active aging longitudinal study. J Strength Cond Res36(4): 1141–1146, 2022—The purpose of the present study was to investigate the effects of resistance training (RT) performed with a higher versus lower training volume on oxidative stress (OS) biomarkers in older women. Thirty-eight older women (≥60 years) were randomly assigned to 1 of 2 groups: a group that performed 1 set per exercise (low volume [LV], n= 18) or 3 sets per exercise (high volume [HV], n= 20). The whole-body RT consisted of a 12-week RT program involving 8 exercises performed with sets of 10–15 repetitions maximum, 3 days per week. Advanced oxidation protein products (AOPP), total radical-trapping antioxidant parameter (TRAP), and ferrous oxidation-xylenol orange (FOX) were used as OS biomarkers. The composite Z-score of the percentage changes from pre- to posttraining of OS biomarkers according to groups was calculated. A significant main effect of time (p< 0.05) was found for AOPP (LV = −7.3% vs. HV = −12.2%) and TRAP (LV = +1.5% vs. HV = +15.5%) concentrations, without a statistical difference between the groups (p> 0.05). A significant group vs. time interaction (p< 0.001) was revealed for FOX (LV = +6.4% vs. HV = −8.9%). The overall analysis indicated higher positive changes for HV than LV (composed Z-score: HV = 0.41 ± 1.22 vs. LV = −0.37 ± 1.03; p< 0.05). Our results suggest that a greater volume of RT seems to promote superior improvements on OS biomarkers in older women.

Background: Many factors may influence the magnitude of individual responses to resistance training (RT). How the manipulation of training volume and frequency affects responsiveness level for muscle mass gain in older women has not been investigated. Aims: This study had the objective of identifying responders (RP) and non-responders (N-RP) older women for skeletal muscle mass (SMM) gain from a 12-week resistance training (RT) program. Additionally, we analyzed whether the N-RP could gain SMM with an increase in weekly training volume over 12 additional weeks of training. Methods: Thirty-nine older women (aged ≥ 60 years) completed 24 weeks of a whole-body RT intervention (eight exercises, 2–3×/week, 1–2 sets of 10–15 repetitions). SMM was estimated by DXA, and the responsive cut-off value was set at two times the standard error of measurement. Participants were considered as RP if they exceeded the cut-off value after a 12-week RT phase, while the N-RP were those who failed to reach the SMM cut-off. Results: Of the 22 participants considered to be N-RP, only 3 accumulated SMM gains (P= 0.250) that exceeded the cut-off point for responsiveness following 12 additional weeks of training, while 19 maintained or presented negative SMM changes. Of the 17 participants considered to be RP, all continued to gain SMM after the second 12-week RT phase. No significant correlation was observed between the changes in SMM and any baseline aspect of the participants. Conclusions: Our results suggest that some older women are RP, while others are N-RP to SMM gains resulting from RT. Furthermore, the non-responsiveness condition was not altered by an increase of training volume and intervention duration while RP participants continue to increase SMM; it appears that RP continue to be RP, and N-RP continue to be N-RP.

Abstract: To date, no meta-analytical study evaluating the benefits of resistance exercise intervention on muscular strength and power and functional capacity in acute hospitalized older adults was conducted. Then, to synthesize the emerging evidence on the effects of resistance exercise intervention on muscular strength and power and functional capacity in acute hospitalized older adults, two independent authors performed a systematic search (PubMed, Scopus, Web of Science, and SciELO) until January 2021. Randomized clinical trials were included regarding the effects of resistance exercise and hospital usual care. The Cochrane Collaboration assessment tool was used to analyze the risk of bias. The comparisons included muscular strength (isometric handgrip strength and one-repetition maximum test of leg press), muscular power (output during leg press exercise), and functional capacity (timed-up-and-go, and short physical performance battery). Resistance exercise intervention increased muscular strength (isometric handgrip strength: mean difference = 2.50 kg, 95% confidence interval (CI) = 1.33, 3.67; and one-repetition maximum test of leg press: mean difference = 19.28 kg, 95% confidence interval = 14.70, 23.86) and muscular power (mean difference = 29.52 W, 95% confidence interval = 28.84, 30.21), and functional capacity (timed-up-and-go: mean difference = 3.40 s, 95% confidence interval = 0.47, 6.36; and short physical performance battery: mean difference = 1.29 points, 95% confidence interval = 0.10, 2.48) at discharge compared with hospital usual care. This meta-analysis endorses the increase of muscular strength and power gains and improving the functional capacity in favor of resistance exercise intervention in acute hospitalized older adults. Trial Registration: https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42020203658

The present study aimed to evaluate the influence of holding the chair handles during the assessment of older women’s knee flexion and extension isokinetic and isometric strength. Of 57 older women (≥60 years) selected from a convenience sample for this research, 23 were randomly chosen to perform the tests twice for reliability analyses. Each participant performed maximal isokinetic knee extension and flexion at 60°/s and 180°/s angular velocities and isometric tests under two conditions: (a) holding the lateral handle of the chair (HOLD) and (b) supporting the hands on the shoulders with the arms crossed (i.e., not holding the handle [NHOLD]). Participants performed significantly better in the HOLD compared with the NHOLD condition. Slightly lower intraclass correlation coefficients (ICC = 0.870–0.956) were noted for the HOLD than for the NHOLD condition (ICC = 0.900–0.981) so that the HOLD performance on Days 1 and 2 was different on some measures. Owing to the higher reliability coefficients in the NHOLD condition, results suggest that, when assessing knee strength in older women, the evaluee should place their hands over their shoulders with arms crossed in front of the chest (NHOLD) to maximize assessment reliability (reproducibility of performance).

Background: Creatine (Cr) supplementation associated with resistance training produces greater muscular strength improvements in the upper compared with the lower body; however, no study has investigated if such region-specific results are seen with gains in muscle mass.Aim: We aimed to evaluate the effect of Cr supplementation in combination with resistance training on lean soft tissue changes in the upper and lower limbs and trunk in resistance-trained young adult men.Methods: In a randomized, double-blind and placebo-controlled design, 43 resistance-trained men (22.7 ± 3.0 years, 72.9 ± 8.7 kg, 177.9 ± 5.7 cm, 23.0 ± 2.5 kg/m2) received either creatine (Cr, n= 22) or placebo (PLA, n= 21) over an 8-week study period. The supplementation protocol included a loading phase (7 days, four doses of 0.3 g/kg per day) and a maintenance phase (7 weeks, single dose of 0.03 g/kg per day). During the same period, subjects performed resistance training four times per week using the following two-way split routine: Monday and Thursday = pectoral, shoulders, triceps, and abdomen, Tuesday and Friday = back, biceps, thighs, and calves. Lean soft tissue of the upper limbs (ULLST), lower limbs (LLLST), and trunk (TLST) was assessed by dual-energy X-ray absorptiometry before and after the intervention.Results: Both groups showed significant (p< 0.001) improvements in ULLST, LLLST, TLST, and the Cr group achieved greater (p< 0.001) increases in these outcomes compared with PLA. For the Cr group, improvements in ULLST (7.1 ± 2.9%) were higher than those observed in LLLST (3.2 ± 2.1%) and TLST (2.1 ± 2.2%). Otherwise, for PLA group there was no significant difference in the magnitude of segmental muscle hypertrophy (ULLST = 1.6 ± 3.0%; LLLST = 0.7 ± 2.8%; TLST = 0.7 ± 2.8%).Conclusion: Our results suggest that Cr supplementation can positively augment muscle hypertrophy in resistance-trained young adult men, particularly in the upper limbs.

Background: Aging is accompanied by progressive and accentuated decline in muscular strength and skeletal muscle mass, affecting health and functional autonomy. Both resistance training (RT) and diet are strategies that may contribute to improvement in the health of the elderly.Aim: The purpose of this study was to evaluate the effects of higher habitual protein intake on RT-induced changes in body composition and strength in untrained postmenopausal women.Methods: Seventy older women were submitted to an RT program. Body composition, muscular strength, and dietary intake (24 h dietary recall) were performed pre- and post-intervention. To verify different intervention effects according to protein intake of the participants, the sample was separated into tertiles according to protein intake: low, moderate, and high protein intake.Results: A time vs. group interaction (p< 0.05) was observed, with high protein intake presenting greater increases compared with low protein intake, for skeletal muscle mass (5.3% vs. 1.3%), lower limb lean soft tissue (4.9% vs. 1.4%), upper lean soft tissue (4.9% vs. 1.2%), preacher curl (24% vs. 15.2%), and total strength (16.4% vs. 11.7%). A time vs. group interaction (p< 0.05) was observed, with high protein intake presenting greater increases compared with moderate protein intake, for skeletal muscle mass (5.3% vs. 3.2%). In all groups, a main effect of time (p< 0.05) was observed for knee extension and chest press.Conclusions: We conclude that intake of >1.0 g/kg/day of protein promotes gains in skeletal muscle mass and muscular strength after RT in untrained older women.

To test the biocultural model through direct and indirect associations between biological maturation, adiposity, cardiorespiratory fitness, feelings of sadness, social relationships, and physical activity in adolescents.

Background: This manuscript describes the design and rationale of a clinical trial that aims to investigate the multiple physiological, attitudinal, nutritional, and behavioral effects of a new interdisciplinary intervention based on the Health at Every Size® (HAES®) approach in obese women.Methods: This will be a prospective, 7-month, randomized (2:1), mixed-method clinical trial. Obese women will be recruited and randomly allocated into two groups. The intervention group (I-HAES®; proposed n= 40) will undertake a novel HAES®-based intervention. Participants will take part in an exercise program, nutrition counseling sessions, and philosophical workshops, all aligned with the principles of the HAES® approach. The control group (CTRL; proposed n= 20) will participate in a program using a traditional HAES®-based group format, characterized by bimonthly lectures about the same topics offered to the experimental group, encouraging the adoption of a healthy lifestyle. The following multiple quantitative outcomes will be assessed pre and post intervention: health-related quality of life, cardiovascular risk factors, anthropometric assessments, physical activity level, physical capacity and function, and psychological and behavioral assessments. Qualitative analysis will be used to evaluate the experiences of the participants throughout the intervention, as assessed by focus groups and semi-structured interviews.Conclusions: The interdisciplinary research team leading this study has varied and complementary expertise. The knowledge arising from this study will help to guide new interdisciplinary interventions with the potential to holistically improve the health of obese individuals. This trial is registered at Clinicaltrials.gov(NCT02102061).

Background: Exercise and supplementation with isoflavones are therapies used to prevent and treat climacteric symptoms.Aim: To verify the effects of 10 weeks of combined aerobic and resistance training and isoflavone supplementation on climacteric symptoms in postmenopausal women.Methods: A randomised, double-blind, controlled clinical trial was performed. A total of 32 postmenopausal women, aged 54.4 ± 5.4 years, with a body mass index of 26.6 ± 3.0 kg/m2and 5.6 ± 4.6 years after menopause, were randomly assigned to groups: placebo and exercise (PLA + EXE, n= 15) or 100 mg of isoflavone and exercise (ISO + EXE, n= 17). At the beginning and after 10 weeks of aerobic + resistance (20 min each, moderate intensity) training, climacteric symptoms were evaluated using the Blatt–Kupperman Menopausal Index, Cervantes Scale and Menopause Rating Scale. ANCOVA was used for analysis between groups and at different times, with the covariate adjusted by the pre-value. The level of significance considered was p< 0.05.Results: A reduction in climacteric symptoms was observed in both groups, without differences between the interventions. The reductions were 45% and 50% for the Blatt–Kupperman Menopausal Index, 41% and 52% for the MRS and 39% and 39% for the Cervantes Scale in the ISO + EXE and PLA + EXE groups, respectively. In the descriptive analysis of the Blatt–Kupperman Menopausal Index values, there was an increase in the absence of symptoms from 48−77% in the ISO + EXE group and 24−58% in the PLA + EXE group.Conclusions: A period of 10 weeks of combined training was effective in improving climacteric symptoms in post-menopausal women. However, isoflavone supplementation did not promote additional effects in improving symptoms.

Background: The constant pursuit of improved athletic performance characterizes high-performance sport and the use of medicinal plants as dietary supplements is becoming widespread among athletes to enhance long-term endurance performance.Aim: The present study evaluated the toxicity of Heteropterys tomentosa(HEHt) and its acute adaptogenic effects.Methods: The in vitro safety profile was evaluated on CHO-k1 cells using the alamar Blue assay, at concentrations ranging from 3.125 to 200 µg/mL. In vivo acute oral toxicity was conducted in male and female mice with oral administration of graded doses of HEHt from 400 to 2000 mg/kg. A subchronic oral toxicity study was completed by oral administration of HEHt (50, 200 or 1000 mg/kg) and vehicle for 30 days in male Wistar rats. Clinical observations and toxicological related parameters were determined. Blood was collected for biochemical and hematological analyses, while histological examinations were performed on selected organs. Thereafter, an adaptogenic test consisting of progressive loads until exhaustion was conducted in rats (n= 5/group) orally pre-treated with the vehicle and HEHt (25, 100 or 400 mg/kg).Results: HEHt exhibited no cytotoxic effects on the CHO-k1 cells and, apparently, no acute toxicity in mice and no subchronic toxicity in rats. An ergogenic effect was observed only at the dose of 25 mg/kg compared with the vehicle in relation to time to exhaustion and exercise load (p= .011 and .019, respectively). HEHt is safe at up to 400 mg/kg, contains astilbin and taxifolin as the major phytochemical compounds, and exhibited a potential adaptogenic effect.Conclusions: These results justify its anecdotal usage as a tonic, show that the hydroethanolic maceration of the root does not cause toxicity, and provide scientific evidence of its potential as a source of new adaptogenic substance(s).

Background: Despite the well-documented importance of nutrition in optimizing performance and health, the dietary intake of soccer players has attracted little attention.Aim: We aimed to assess the pre-season dietary intake of professional soccer players and its adequacy in macro and micronutrients.Methods: The pre-season dietary intake of 19 male athletes was assessed using a semi-structured 3-day food record. To determine dietary adequacy and excess, energy and macronutrient intake were compared with the Brazilian dietary reference values for athletes, and micronutrients were compared with the Estimated Average Requirement – EAR (minimum recommendation) and Tolerable Upper Intake Level – UL (maximum recommendation).Results: Mean daily energy intake (40.74±12.81 kcal/kg) was adequate. However, there was a low carbohydrate intake (5.44±1.86 g/kg/day) and a high amount of protein and fat (1.91±0.75 and 1.27±0.50 g/kg/day, respectively). Sodium intake (3141.77±939.76 mg/day) was higher than UL (2300 mg/day), while the majority of players showed daily intake of vitamin A (74%), vitamin D (100%), folate (58%), calcium and magnesium (68%) below the EAR (625, 10 and 320 µg/day, 800 and 330 mg/day, respectively).Conclusion: The dietary intake of professional soccer players was adequate in energy, but inadequate in macro and micronutrients, which suggests the need to improve nutritional practices to sustain the physical demands of soccer during pre-season.

Background: Probiotics are live microorganisms that promote health benefits to the host. Evidence indicates that some probiotic strains play an immunomodulatory role and reduce the incidence of respiratory and gastrointestinal infections in athletes and in physical activity practitioners. For this reason, probiotic supplementation could indirectly improve exercise performance. However, recent studies have observed direct ergogenic effects of probiotics, but the mechanisms of action are poorly elucidated.Objective: In this study, we aim to synthesize available knowledge on the effect of probiotics on physical exercise, identify the mechanisms of action by which probiotics could improve performance directly and indirectly, and verify whether probiotics have any ergogenic effect.Methods: The study was performed in the PubMed database in February 2017, without limitation as to the publication period. The keyword combinations used were: ‘Probiotics’ and ‘Sports’ (n= 17 articles), ‘Probiotics’ and ‘Exercise’ (n= 26 articles) and ‘Probiotics’ and ‘Athletes’ (n= 11 articles).Results: Of the 16 studies evaluated, only six applied performance tests, of which only two demonstrated that probiotic supplementation increases performance, but one of them was performed with mice.Conclusions: According to the studies evaluated, probiotic supplementation does not present ergogenic effect, however, considering the small number of studies, this subject should be better investigated.

Background: Ergogenic effects of caffeine (CAF) ingestion have been observed in different cycling exercise modes, and have been associated with alterations in ratings of perceived exertion (RPE). However, there has been little investigation of maximal oxygen uptake (VO2MAX) test outcomes.Aim: This study aimed to verify whether CAF may reduce RPE, thereby improving maximal incremental test (MIT) outcomes such as VO2MAX, time to exhaustion and peak power output (WPEAK).Methods: Nine healthy individuals performed three MITs (25 W/min until exhaustion) in a random, counterbalanced fashion after ingestion of CAF, placebo perceived as caffeine (PLA), and no supplementation (baseline control). VO2was measured throughout the test, while RPE was rated according to overall and leg effort sensations. The power output corresponding to submaximal (RPE = 14 according to the 6–20 Borg scale) and maximal RPE was recorded for both overall (O-RPE14and O-RPEMAX) and leg RPE (L-RPE14and L-RPEMAX).Results: VO2MAXdid not change significantly between MITs; however, CAF and PLA increased time to exhaustion (↑ ∼18.7% and ∼17.1%, respectively; p< .05) and WPEAK(↑ ∼13.0% and ∼11.8%, respectively; p< .05) when compared with control. When compared with control, CAF ingestion reduced submaximal and maximal overall and leg RPEs, the effect being greater in maximal (likely beneficial in O-RPEMAXand L-RPEMAX) than submaximal RPE (possibly beneficial in O-RPE14and L-RPE14). Similar results were found when participants ingested PLA.Conclusions: Compared with control, CAF and PLA improved MIT performance outcomes such as time to exhaustion and WPEAK, without altering VO2MAXvalues. CAF effects were attributed to placebo.

Background: Despite the positive effects of high-intensity training on weight management and health-related outcomes, it is postulated that high-intensity training may also induce oxidative stress, increasing hepatic damage.Aim: The aim of this study was to compare the effects of low versus high-intensity training on biomarkers related to non-alcoholic fatty liver disease (NAFLD) in adolescents with obesity.Methods: For this study 107 adolescents (15 ± 1 years) with obesity (BMI = 34.7 ± 4.1 kg/m2) were randomized into High-Intensity Training (HIT, n= 31), Low-Intensity Training (LIT, n= 31) or Control Group (CG, n= 45). Adolescents from HIT and LIT received nutritional, psychological and clinical counseling. Blood lipids, Castelli risk index, glucose, insulin and hepatic enzymes were measured at baseline and after 12 weeks.Results: Castelli risk index 1 was reduced in all groups (p< 0.001) with moderate effect size (d) for HIT (d= 0.62) and LIT (d= 0.66). Castelli risk index 2 also decreased (p< 0.001 for all groups; HIT d= 0.65; LIT d= 0.79). High-density lipoprotein increased in all groups (d= 0.25 and d= 0.18 in HIT and LIT), while alanine aminotransferase tended to reduce (p= 0.062) in HIT (d= 0.34) and LIT (d= 0.73) and aspartate aminotransferase decreased (p= 0.024) in both HIT (d= 0.24) and LIT (d= 0.45). There were no changes in glucose, insulin and insulin resistance.Conclusion: Both high and low-intensity training improved biomarkers related to NAFLD. Thus, high-intensity training can be a safe and effective alternative to prevent and treat NAFLD in adolescents with obesity.

Background: Frailty is a multifactorial geriatric syndrome characterized by progressive decline in health and associated with decreased muscle mass, strength, and functional capacity. Resistance training (RT) combined with protein or amino acids supplementation has been shown to be promising for mitigating age-related impairments.Aim: To investigate the chronic effects of different strategies of protein and derivatives supplementation in association with RT on selected health-related parameters in pre-frail and frail elderly.Methods: This is a series of double-blind, randomized, placebo-controlled, parallel-group clinical trials. Volunteers will be divided into nine groups, comprising four different sub-studies evaluating the effects of: isolated leucine supplementation (study 1); protein source (whey vs. soy – study 2); combination of whey protein and creatine (study 3); and sexual dimorphism on the response to protein intake and RT (males vs. females – study 4). Muscle cross-sectional area, fiber cross-sectional area, body composition, lower-limb maximal dynamic and isometric strength, functionality, lipid profile, biochemical parameters, renal function, quality of life, and nutritional status will be assessed before and after a 16-week intervention period. Data will be tested for normality and a mixed-model for repeated measures will be conducted to assess within- and between-group effects of the intervention on the dependent variables. Confidence intervals (95%), effect sizes, and relative changes will also be determined, with significance set at p< 0.05.

AbstractThe goal of the present study was to analyse the impact of rapid weight loss methods (10%) on physical performance of judo athletes. Thirty-nine adult male judo athletes were selected to participate in the present study. Athletes were randomly allocated in two groups: experimental (EG, n = 20) and control (CG, n = 19). Twenty-four hours before the beginning of experimental approach, athletes were submitted to anthropometrical measurements and performed the Special Judo Fitness Test. The weight loss in the EG was conducted to generate a mean loss of 5% per week during 2 weeks. A two-way analysis of variance with repeated measurements was conducted to compare groups and moments. There was a group and moment interaction (p = .001) for body mass, with decrease only for the EG (Δ% = −10.6%, p = .01). There was also a group and moment for the Special Judo Fitness Test index (p = .001), with an improvement only in the CG (Δ% = −6%, p = .01). It was concluded that the rapid weight loss process was not a good strategy for judo athletes’ performance optimisation.

AbstractBackground:Earlier biological maturation has been related to increased metabolic risk. In this study, we verified mediating effects by central adiposity of the relationship between somatic maturity and metabolic risk factors in adolescents.Methods:In a cross-sectional study, 1034 adolescents aged 10–16 years from Londrina/PR/Brazil were evaluated. The age of peak height velocity (PHV) method was used to evaluate somatic maturity. Central adiposity was estimated through waist circumference measurements. Fasting glucose, triglycerides, high-density lipoprotein cholesterol (HDL-C), and blood pressure were measured as metabolic risk indicators. Physical activity (Baecke questionnaire) and cardiorespiratory fitness (20-m shuttle run test) were used as covariates.Results:Except for fasting glucose, waist circumference showed partial or full mediation of the relationship between maturity and the following metabolic risk factors with their respective z-score values: triglycerides (boys = −3.554 vs. girls = −5.031), HDL-C (boys = +5.300 vs. girls = +5.905), systolic blood pressure (boys = −3.540 vs. girls = −3.763), diastolic blood pressure (boys = −2.967 vs. girls = −3.264), and metabolic risk score (boys = −5.339 vs. girls = −6.362).Conclusions:The results suggest that central obesity plays a mediating role in the relationship between somatic maturation and metabolic risk during adolescence.

The indiscriminate use of different diagnostic criteria for the definition of dyslipidemia may result in inaccurate interpretations, which could compromise diagnosis and therefore the therapeutic and prophylactic actions to be taken.

Warm-up has been shown to mediate numerous acute physiological alterations that have been purported to confer beneficial effects on performance. This study investigated the acute effects of different warm-up procedures on resistance training performance. Employing a randomized, counterbalanced crossover design, 15 men performed 3 exercises (4 sets of bench press, squat, and arm curl at 80% of 1RM) to failure in 4 conditions (control, specific, aerobic, and combined). Outcome measures included the sum of repetitions and a fatigue index measuring the decline between sets. There was no significant difference for the sum of repetitions or for fatigue index among conditions for the 3 exercises. Performance in the resistance training exercises was not influenced by warm-up.

Analisar a validade de métodos para avaliação de gordura corporal em crianças e adolescentes.

To analyze the validity of methods to assess body fat in children and adolescents using a systematic review.

This investigation analyzed the effect of rest interval length on the rating of perceived exertion (RPE) during a resistance exercise session. Nineteen males performed two experimental sessions: resistance exercise with 30-sec. rest intervals (E30) and 90-sec. rest intervals (E90). In both sessions, five exercises (bench press, knee extension, seated row, knee curl, and frontal rise) were performed at 50% 1RM in three sets of 12, 9, and 6 repetitions, respectively. In the E30 session, the RPE increased between sets in all exercises, while in the E90 session, the RPE increased from the first set to the second set in three exercises. RPE in the E30 session was higher than that in the E90 session in the third set. The results suggest that RPE increases for shorter rest intervals than for longer rest intervals. Therefore, the RPE could be considered an indicator of muscle recovery during resistance exercise.