Search

Your search keyword '"Díaz-Ruíz, J. R."' showing total 4 results
Start Over Author "Díaz-Ruíz, J. R." Database Supplemental Index
4 results on '"Díaz-Ruíz, J. R."'

1. The nucleotide sequence of the coat protein genes and 3′ non-coding regions of two resistance-breaking tobamoviruses in pepper shows that they are different viruses

Author

García-Luque, I., Ferrero, M. L., Rodríguez, J. M., Alonso, E., de la Cruz, A., Sanz, A. I., Vaquero, C., Serra, M. T., and Díaz-Ruíz, J. R.

Abstract

The nucleotide sequence of the coat protein genes and 3′ non-coding regions of two different resistance-breaking tobamoviruses in pepper have been determined. The deduced coat protein of an Italian isolate of pepper mild mottle virus (PMMV-I) consists of 156 amino acids and its 3′ non-coding region is 198 nucleotides long. They have been found to be very similar in sequence and structure to those previously reported for a Spanish isolate (PMMV-S). In contrast, a Dutch isolate termed P 11 codes for a coat protein of 160 amino acids and its 3′ non-coding region is 291 nucleotides long, which may have arisen by duplication. The nucleotide and the predicted coat protein amino acid sequence analysis show that this isolate should be considered as a new virus within the tobamovirus group. The term paprika mild mottle virus (PaMMV) is proposed.

Published
1993
Full Text
View/download PDF

2. Nicotiana benthamiana Plants Transformed with the 54-kDa Region of the Pepper Mild Mottle Tobamovirus Replicase Gene Exhibit Two Types of Resistance Responses against Vital Infection

Author

Tenllado, F., García-Luque, I., Serra, M. T., and Díaz-Ruíz, J. R.

Abstract

Nicotiana benthamiana plants transformed with the 54-kDa region of the pepper mild mottle tobamovirus (PMMV) replicase gene were generated and six independently transformed plant lines were analyzed for resistance to PMMV. Two different resistance responses were obtained. Some of the transgenic plants from only two lines showed a preestablished, complete, and highly resistant phenotype since no vital symptoms were observed, although a low level of virus replication occurred The remaining plants from these two lines and all of the plants from the other four lines tested showed a delayed, induced, and also highly resistant phenotype since they were susceptible early, but were able to recover from the systemic PMMV infection. Recovered, symptomless leaves were resistant to the PMMV strain from which the 54-kDa gene was derived and to a closely related strain but not to tobacco mosaic virus. Such a delayed resistance phenotype has not been previously described for any plant expressing viral replicase sequences. The transgenic plants within the lines displaying complete or delayed resistance phenotypes were analyzed for transgene expression before and after PMMV inoculation and the two types of resistance responses were shown to be independent of the transgene transcript level. Copyright 1995, 1999 Academic Press

Published
1995
Full Text
View/download PDF

3. Abstracts of presentations on plant protection issues at the xth international congress of virology

Author

Liu, Sijun, Bedford, Ian D., Markham, Peter G., Ghanim, Morad, Zeidan, Muhamad, Czosnek, Henryk, Bruyère, A., Herrbach, E., Brault, V., Ziegler-Graff, V., Guilley, H., van den Heuvel, J. F. J. M., Taiwo, M. A., Dijkstra, J., Martinez, B., López-Moya, J. J., Llave, C., Díaz-Ruíz, J. R., López-Abella, D., Mikoshiba, Y., Honda, K., Kanematsu, S., Fujisawa, I., Salomon, Raffi, Bernardi, Francoise, Raccah, B., Singer, S., Gal-On, A., Huet, H., López-Moya, J. J., Pirone, T. P., Visser, Peter B., Bol, John F., Hernández, Carmen, Brown, Derek J. F., Pappu, H. R., Culbreath, A. K., Todd, J. W., McPherson, R. M., Sherwood, J. L., Bertrand, P. F., Robbins, M. A., Reade, R. D., Rochon, D. M., Schönfelder, M., Körbler, M., Barg, E., Lesemann, D. -E., Vetten, H. J., Manqussopoulos, I. N., Tsagris, M., Maiss, E., Marczewski, W., Syller, J., Romero, Javier, Molina-Garcia, Antonio, Babin, Mar, Bujarski, Jozef J., Pogany, Judy, Zhang, L., Palukaitis, P., Kaplan, I. B., Qu, Feng, Morris, T. Jack, Steinkellner, H., Puehringer, H., Machado, A. M. Laimer da Câmara, Hammond, J., Brandt, S., Katinger, H., Himmler, G., Carrier, K., Hans, F., Wang, A., Sanfacon, H., Palkovics, László, Balázs, Ervin, Petrzik, K., Mráz, I., Fránová-Honetšlegrová, J., Kusiak, C., Berthome, R., Dinant, S., Astier, S., Albouy, J., Renou, J. P., Bó, E. Dal, Torre, M. E. Sánchez de la, Djelouah, K., García, M. L., Grau, O., Benvenisti, Luna, Gelman, Boris, Hai, Dalia, Yadin, Hagai, Stram, Yehuda, Becker, Yechiel, Čeřovská, N., Filigarová, M., Dědič, P., Nemchinov, L., Hadidi, A., Choi, Y. G., Randles, J. W., Samson, A. C. R., Wilford, J. N., Chapman, S., Santa Cruz, S., Wilson, T. M. A., Wilkinson, Nicola, Wilson, Louise, Marlow, Susan, King, Linda, Possee, Robert, Zhu, Fanxiu, Qi, Yipeng, Huang, Yongxiu, Hu, Jianhong, Oker-Blom, Christian, Keinänen, Kari, Chauhan, B. K., Possee, R. D., French, T. J., Finkelstein, Y., Levi, B. Z., Faktor, O., Toister-Achituv, Mira, Wang, Fushan, Qi, Yipeng, Huang, Yongxiu, Lu, Liquan, Du, Quansheng, Watson, S. K., Kalmakoff, J., Broer, R., Liu, Y., Zuidema, D., Strien, E. A. van, Vlak, J. M., Heldens, J. G. M., Chejanovsky, N., Gershburg, E., Toister-Achituv, Mira, Faruchi, S., Kamensky, B., Faktor, O., Faktor, O., Nahum, O., Stockholm, D., Rivkin, H., Gurevitz, M., Chejanovsky, N., Zilberberg, N., Gershburg, E., Stockholm, D., Rivkin, H., Chejanovsky, N., Gurevitz, M., Zilberberg, N., Gershburg, E., Smith, P., King, L. A., Bamett, A., Windass, J. D., Possee, R. D., Jacobs, C., Fielding, B., Davison, S., Kunjeku, E., Guarino, L. A., Jarvis, D. L., Reilly, L., Hoover, K., Schultz, C. M., Hammock, B. D., Gordon, K. H. J., Bawden, A. L., Brooks, E. M., Lincoln, M. R., Hanzlik, T. N., Larkin, P. J., Gordon, K. H. J., Bawden, A. L., van Hulten, M. C. W., Hanzlik, T. N., Hendry, D. A., Stephens, Rachel, Barnett, Anna, Thomas, Carole, Possee, Robert, King, Linda, Phanis, Constantinos, O’Reilly, David R., Clarke, E., Tristem, Michael, Cory, Jennifer, O’Reilly, David R., Mayo, M. A., Duncan, G. H., Reavy, B., Gildow, F. E., Lamb, J. W., Hay, R. T., Li, Shoudong, Qi, Bing, Wang, Jiawang, Qi, Yipeng, O’Reilly, David R., Kang, WonKyung, Crook, Norman E., Winstanley, Doreen, Alaoui-Ismaili, M. H., Richardson, C. D., Lundsgaard, T., Kobayashi, J., Kayama, T., Ikeda, N., Miyajima, S., Inouye, K., Kimura, T., Suzuki, N., Sugawara, M., Nuss, D. L., Matsuura, Y., Simón, Laureano, Guo, Huishan, García, Juan Antonio, Wang, S., Miller, W. A., Browning, K., Fütterer, Johannes, Potrykus, Ingo, Bao, Yiming, Li, Liu, Burns, Thomas M., Hull, Roger, Hohn, Thomas, Hefferon, K. L., AbouHaider, M. G., Hulanicka, D., Juszczuk, M., Iskakov, B. K., Shmanov, M. A., Polimbetova, N. S., Zhanybekova, S. Sh., Lee, A. V., Galiakparov, N. N., Dale, J. L., Beetham, P. R., Hafner, G. J., Harding, R. M., and Dale, J. L.

Published
1998
Full Text
View/download PDF

4. Nucleotide sequence and symptom modulating analysis of a Peanut stunt virus-associated satellite RNA from Poland: high level of sequence identity with the American PSV satellites

Author

Ferreiro, C., Ostrówka, K., López-Moya, J. J., and Díaz-Ruíz, J. R.

Abstract

Two peanut stunt virus isolates from Poland (PSV-Ag and PSV-P) have been studied. The isolates produce similar systemic symptoms onNicotiana tabacumplants but the symptoms onN. benthamiana, Pisum sativumandDatura stramoniumplants are much stronger for the PSV-P isolate. Analysis of the RNA extracted from purified virions by gel electrophoresis and RT-PCR amplification allowed the detection of a satellite RNA in the PSV-P isolate. The nucleotide sequence of this European PSV satellite was determined and found to have a high degree of identity with the sequences of the four American PSV satellites previously studied, which were found to have either no effect or ameliorate the PSV symptoms in tobacco plants (Collmer et al., 1985; Naidu et al., 1991). The possible role of the European PSV satellite in the modulation of viral symptoms has been studied but no effect was observed when the purified satellite was used with the PSV-Ag isolate as helper virus on any of the three hosts cited above.

Published
1996
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results