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The endothelin (ET) receptor that mediates vasoconstriction of the isolated rabbit pulmonary resistance artery was characterized using selective ET receptor agonists and antagonists. We also examined changes in ET-induced vasoconstriction brought about by left ventricular dysfunction using the rabbit coronary ligation model. The rank order of potency for contraction was sarafotoxin S6c (S6c) > ET-1 = ET-3, which is characteristic of an ETB-like receptor. The combined ETA/ETB receptor antagonist SB209670 (1 microM) antagonized responses to ET-1 and S6c with estimated pKb values of 6.8 +/- 0.2 and 7.8 +/- 0.2, respectively. BQ788 (1 microM) antagonized responses to S6c and ET-3 (but not ET-1) with estimated pKb values of 7.1 +/- 0.2 and 6.6 +/- 0.1, respectively. The ETA receptor antagonist FR139317 (1 microM), either alone or in combination with BQ788, did not inhibit responses to ET-1. The profile of the ET-1 response was not altered by left ventricular dysfunction. In control rabbits, the inhibitor of nitric oxide synthase N omega-nitro-L-arginine methyl ester (100 microM) had no significant effect on the potency of either ET-1 or S6c. In the coronary-ligated rabbits, however, it significantly increased the potency (10-15-fold) of both ET-1 and S6c. These results suggest that the ET receptor that mediates contraction in rabbit pulmonary resistance arteries has the characteristics of an ETB-like receptor. The responses to ET-1 are not altered by LVD but may be modified by increased release of nitric oxide.

Thedietsofthealpinegalaxias(Galaxias paucispondylus Stokell, 1938) and the longjawed galaxias (G. prognathus Stokell, 1940) were studied in Deep Creek, a high-country stream in the central South Island of New Zealand. Both species are small, slender fish with entirely freshwater life cycles. Their diets were very similar and consisted of aquatic invertebrates, dominated by the larvae of Deleatidium spp. (Ephemeroptera), Hydrobiosis spp. (Trichoptera), and Chironomidae (Diptera). Although diets partly reflected what was available in the stream, both fish selected soft-bodied prey in preference to cased or harder shelled prey. Both species are probably nocturnal feeders.

Populations of small fish were sampled in 12-20 riffles of the lower reaches of 3 braided rivers in Canterbury, New Zealand, during periods of low, stable flows. In the Ashley, which has been least affected by floods in recent years, the standing stock of fish was severalfold higher than in the Hurunui and Rakaia Rivers, which experienced large floods over much of the time, particularly the Rakaia River. Mean abundance + 1 standard deviation (S.D.) of all species combined, amounted to 5.95 + 2.76 fish m 2 for 10 species in the Ashley, 0.59 + 0.60 fish m 2 for 7 species in the Hurunui, and 0.23 + 0.11 fish m 2for 6 species in the Rakaia. Mean biomass was 24.85 + 9.59, 2.11 + 1.19, and 2.50 ± 3.60 g m 2in the Ashley, Hurunui, and Rakaia Rivers, respectively. The more common species in the Ashley and Rakaia Rivers were torrentfish, longfinned eel, blue-gilled bully, and upland bully. The same dominant species complex prevailed in the Hurunui River except that the common river galaxias was present instead of the bluegilled bully. The adverse effects of floods on fish, either directly, indirectly, or both, are considered to be a major limiting factor offish populations in braided rivers characterised by highly unstable flows and river beds, and a lack of suitable cover for fish.

Torrentfish Cheimarrichthys fosteri Haast were collected at 4-hour intervals over a full day in winter and summer, 1984-85, from the Ashley and Rakaia Rivers in Canterbury, New Zealand, to determine their diel feeding periodicity. Both populations fed, almost exclusively, from dusk to dawn in both seasons, with the quantity of food in their stomachs accumulating to peak levels by early morning. However, Ashley females had significantly less food in their stomachs than did males in summer, the season with a high incidence (91%) of females in spawning condition. Aquatic stages of Deleatidium spp. (Ephemeroptera: Leptophle-biidae) and Chironomidae constituted from 85 to 98% by number and 63 to 80% by dry weight of all foods eaten. Chironomids (small prey) comprised up to 85% of the total foods eaten by number but only 17% by dry weight, while Deleatidium (large prey) comprised up to 69% of the prey by number and 63% by dry weight. Numerically, chironomids were an important component in the torrentfish diet in both seasons in the Rakaia (71 to 75%), but only so in winter in the Ashley (85%). The reported daily feeding cycle is consistent with observations in a simulated stream, that torrentfish largely remain inactive during the day and come out to feed at night.

Selective ETAand ETBreceptor antagonists do not fully inhibit responses to ET-1 in human small pulmonary arteries. Here, we have compared the influence of the novel mixed ETA/ETBantagonists SB/217242, SB/234551 and SB/209670 on ET-1-mediated vasoconstriction in these vessels. ET-1 was a potent vasoconstrictor (pEC50: 8.14±0.05, n=5) and the concentration-response curve to ET-1 was biphasic in nature. All three mixed antagonists (1 μM) inhibited the responses to ET-1, abolishing the biphasic form of the concentration response curve. The order of potency was SB 209670>SB 234551≥SB 217242 with estimated pKb values of 8.0±0.20, 6.8±0.17 and 6.6±0.11 respectively (n=3–5).