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The article focuses on a research on role of alanine in catalytic activity of diadenosine tetraphosphate. Topics discussed include need for anti-tuberculosis drugs due to the increasing threat of tuberculosis across the world, use of QuickChange Primer Design Program from analytical tools provider Agilent Technologies to design Ala-149 deletion primer and the Ala-149 deletion antisense primer and correlation of Ala-149 loop deletion from active site of MtAPA to substrate specificity.