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A 12-year-old intact male Miniature Schnauzer dog with chronic diarrhea that was unresponsive to empirical treatment was presented to a referring veterinarian. A laparotomy was performed, and formalin-fixed biopsies of duodenum, jejunum, and colon were sent to Oklahoma Animal Disease Diagnostic Laboratory for evaluation. Histologic examination revealed a severe, diffuse, granulomatous enteritis and colitis with intralesional yeast and hyphal forms. Grocott methenamine silver stains revealed short, aseptate hyphae co-mingled with 2–8 µm, oval to round yeast organisms consistent with Histoplasma capsulatum. The atypical presentation of both yeast and hyphal forms prompted identification of the organism. Direct sequencing of a polymerase chain reaction product from paraffin-embedded intestinal samples confirmed the presence of Ajellomyces capsulatuswith a homology over 99% to several sequences in GenBank. Ajellomyces capsulatusis the holomorphic name for H. capsulatum. Therefore, the mycelial form of a dimorphic fungus such as H. capsulatumcan coexist with yeast cells within lesions of histoplasmosis. Following diagnosis, the dog was treated with itraconazole for 6 months and has improved.

Pesticide use by agricultural producers is continually being scrutinized, and regulations concerning pesticide use are ever increasing. This study examines the perceived educational needs of Wyoming farmers and ranchers. A questionnaire was administered to approximately 2500 producers in January of 1995. Respondents identified and ranked the information sources they used most commonly in making their pesticide decisions. Respondents also indicated their agreement or disagreement with four statements designed to obtain their feelings concerning educational needs, pesticide dangers, and pesticide use. A qualitative response model was developed to help identify the demographic and socioeconomic characteristics of respondents who felt that more educational programs on the proper utilization of pesticides should be provided. The Weed and Pest District was the most commonly utilized source of pesticide information, followed by veterinarians, county extension agents, chemical companies, and neighbors. Almost 75% of respondents agreed with the statement that more educational programs should be provided on the proper application and use of pesticides. Results of the qualitative response model indicate producers with lower income levels (<$20 000) did not feel a need for additional education programs. Producers concerned about the effects of pesticides on the environment and their own health were more apt to feel a need for additional education programs. Results indicate that producers recognize a need for pesticide training and opportunities exist to provide additional training programs to a receptive clientele.