1. Occurrence of Colletotrichum fruticolaalong with C. gloeosporioidesin causing anthracnose disease on Citrus sinensisin Tunisia
- Author
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Ben Hadj Daoud, Haïfa, Ben Salem, Ibtissem, Sánchez, José, Gallego, Eduardo, and Boughalleb-M’Hamdi, Naima
- Abstract
Information on the Colletotrichumspecies associated with Citrus anthracnose was lacking. Surveys were conducted from January 2012 to February 2013 on different citrus species and varieties orchards in the Cap-Bon region (Tunisia) to investigate the prevalence of Colletotrichumspecies. Typical anthracnose symptoms such as dieback and wither-tip of twigs were noticed. The fungi associated with anthracnose were isolated and studied. Morphological features (mycelial and sporulation characterization), molecular identification by using ITS1 and ITS2 primers were done along with pathogenicity test monosporic cultures. Eight Colletotrichumisolates were collected from infected fruits, leaves and twigs and were identified as Colletotrichum gloeosporioides(seven isolates) and Colletotrichum fructicola(one isolate). All eight Colletotrichumisolates were pathogenic on Thomson, Malti and Meski orange varieties, Lunari lemon variety and sweet lime Bergamot detached fruit and leaves. This investigation revealed the prevalence, for the first time of C. fructicola, on Citrus in Tunisia.
- Published
- 2019
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