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Species identification lies at the heart of biodiversity studies that has in recent years favoured DNA-based approaches. Microbial Biological Resource Centres are a rich source for diverse and high-quality reference materials in microbiology, and yet the strains preserved in these biobanks have been exploited only on a limited scale to generate DNA barcodes. As part of a project funded in the Netherlands to barcode specimens of major national biobanks, sequences of two nuclear ribosomal genetic markers, the Internal Transcribed Spaces and 5.8S gene (ITS) and the D1/D2 domain of the 26S Large Subunit (LSU), were generated as DNA barcode data for ca. 100 000 fungal strains originally assigned to ca. 17 000 species in the CBS fungal biobank maintained at the Westerdijk Fungal Biodiversity Institute, Utrecht. Using more than 24 000 DNA barcode sequences of 12 000 ex-type and manually validated filamentous fungal strains of 7 300 accepted species, the optimal identity thresholds to discriminate filamentous fungal species were predicted as 99.6 % for ITS and 99.8 % for LSU. We showed that 17 % and 18 % of the species could not be discriminated by the ITS and LSU genetic markers, respectively. Among them, ∼8 % were indistinguishable using both genetic markers. ITS has been shown to outperform LSU in filamentous fungal species discrimination with a probability of correct identification of 82 % vs. 77.6 %, and a clustering quality value of 84 % vs. 77.7 %. At higher taxonomic classifications, LSU has been shown to have a better discriminatory power than ITS. With a clustering quality value of 80 %, LSU outperformed ITS in identifying filamentous fungi at the ordinal level. At the generic level, the clustering quality values produced by both genetic markers were low, indicating the necessity for taxonomic revisions at genus level and, likely, for applying more conserved genetic markers or even whole genomes. The taxonomic thresholds predicted for filamentous fungal identification at the genus, family, order and class levels were 94.3 %, 88.5 %, 81.2 % and 80.9 % based on ITS barcodes, and 98.2 %, 96.2 %, 94.7 % and 92.7 % based on LSU barcodes. The DNA barcodes used in this study have been deposited to GenBank and will also be publicly available at the Westerdijk Institute's website as reference sequences for fungal identification, marking an unprecedented data release event in global fungal barcoding efforts to date.

Abstract.: Using a perturbative model for diffractive interactions, we derive an expression for the polarized diffractive structure function in the high energy limit. This structure function is given by the interference of diffractive amplitudes with polarized and unpolarized exchanges. For the polarized exchange we consider both two-gluon and quark-antiquark amplitudes. The polarized diffractive amplitude receives sizable contributions from non-strongly ordered regions in phase space, resulting in a double logarithmic enhancement at small x. The resummation of these double logarithmic terms is outlined. We also discuss the transition from our perturbative expression to the nonperturbative region. A first numerical estimate indicates that the perturbative contribution to the spin asymmetry is substantially larger than the nonperturbative one.

In the past year, polarized deep inelastic scattering experiments at CERN and SLAC have obtained structure function measurements off proton, neutron and deuteron targets at a level of precision never before achieved. The measurements can be used to test the Bjorken and Ellis-Jaffe sum rules, and also to obtain information on the parton distributions in polarized nucleons. We perform a global leading-order QCD fit to the proton deep inelastic data in order to extract the spin-dependent parton distributions. By using parametric forms which are consistent with theoretical expectations at large and smallx, we find that the quark distributions are now rather well constrained. We assume that there is no significant intrinsic polarization of the strange quark sea. The data are then consistent with a modest amount of the proton's spin carried by the gluon, although the shape of the gluon distribution is not well constrained, and several qualitatively different shapes are suggested. The spin-dependent distributions we obtain can be used as input to phenomenological studies for future polarized hadron-hadron and lepton-hadron colliders.

The idea that the pomeron has partonic structure similar to any other hadron has been given strong support by recent measurements of the diffractive structure function at HERA. We present a detailed theoretical analysis of the diffractive structure function under the assumption that the diffractive cross section can be factorized into a pomeron emission factor and the deep inelastic scattering cross section of the pomeron. We pay particular attention to the kinematic correlations implied by this picture, and suggest the measurement of an angular correlation which should provide a first test of the whole picture. We also present two simple phenomenological models for the quark and gluon structure of the pomeron, which are consistent with various theoretical ideas and which give equally good fits to recent measurements by the H1 collaboration, when combined with the pomeron emission factor of Donnachie and Landshoff. We predict that a large fraction of diffractive deep inelastic events will contain charm, and discuss how improved data will be able to distinguish the models.