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Isthmic progenitors, tissue-specific stem cells in the stomach corpus, maintain mucosal homeostasis by balancing between proliferation and differentiation to gastric epithelial lineages. The progenitor cells rapidly adopt an active state in response to mucosal injury. However, it remains unclear how the isthmic progenitor cell niche is controlled during the regeneration of damaged epithelium. We recapitulated tissue recovery process after acute mucosal injury in the mouse stomach. Bromodeoxyuridine incorporation was used to trace newly generated cells during the injury and recovery phases. To define the epithelial lineage commitment process during recovery, we performed single-cell RNA-sequencing on epithelial cells from the mouse stomachs. We validated the effects of amphiregulin (AREG) on mucosal recovery, using recombinant AREG treatment or AREG-deficient mice. We determined that an epidermal growth factor receptor ligand, AREG, can control progenitor cell lineage commitment. Based on the identification of lineage-committed subpopulations in the corpus epithelium through single-cell RNA-sequencing and bromodeoxyuridine incorporation, we showed that isthmic progenitors mainly transition into short-lived surface cell lineages but are less frequently committed to long-lived parietal cell lineages in homeostasis. However, mucosal regeneration after damage directs the lineage commitment of isthmic progenitors towards parietal cell lineages. During recovery, AREG treatment promoted repopulation with parietal cells, while suppressing surface cell commitment of progenitors. In contrast, transforming growth factor-α did not alter parietal cell regeneration, but did induce expansion of surface cell populations. AREG deficiency impairs parietal cell regeneration but increases surface cell commitment. These data demonstrate that different epidermal growth factor receptor ligands can distinctly regulate isthmic progenitor-driven mucosal regeneration and lineage commitment. [Display omitted] Lineage commitment of stomach isthmic progenitor cells is regulated by different ligands for the epidermal growth factor receptor. [ABSTRACT FROM AUTHOR]

Elements of field cancerization, including atrophic gastritis, metaplasia, and dysplasia, promote gastric cancer development in association with chronic inflammation. However, it remains unclear how stroma changes during carcinogenesis and how the stroma contributes to progression of gastric preneoplasia. Here we investigated heterogeneity of fibroblasts, one of the most important elements in the stroma, and their roles in neoplastic transformation of metaplasia. We used single-cell transcriptomics to evaluate the cellular heterogeneity of mucosal cells from patients with gastric cancer. Tissue sections from the same cohort and tissue microarrays were used to identify the geographical distribution of distinct fibroblast subsets. We further evaluated the role of fibroblasts from pathologic mucosa in dysplastic progression of metaplastic cells using patient-derived metaplastic gastroids and fibroblasts. We identified 4 subsets of fibroblasts within stromal cells defined by the differential expression of PDGFRA , FBLN2, ACTA2 , or PDGFRB. Each subset was distributed distinctively throughout stomach tissues with different proportions at each pathologic stage. The PDGFRα+ subset expanded in metaplasia and cancer compared with normal, maintaining a close proximity with the epithelial compartment. Co-culture of metaplasia- or cancer-derived fibroblasts with gastroids showing the characteristics of spasmolytic polypeptide-expressing metaplasia–induced disordered growth, loss of metaplastic markers, and increases in markers of dysplasia. Culture of metaplastic gastroids with conditioned media from metaplasia- or cancer-derived fibroblasts also promoted dysplastic transition. These findings indicate that fibroblast associations with metaplastic epithelial cells can facilitate direct transition of metaplastic spasmolytic polypeptide-expressing metaplasia cell lineages into dysplastic lineages. [Display omitted] Altered stromal fibroblasts in the precancerous gastric lining promote the transition of precancerous metaplastic cells into dysplasia, supporting the development of gastric cancer. [ABSTRACT FROM AUTHOR]

Blunt cerebrovascular injury (BCVI) is a rare finding in trauma patients. The previously validated BCVI (Denver and Memphis) prediction model in adult patients was shown to be inadequate as a screening option in injured children. We sought to improve the detection of BCVI by developing a prediction model specific to the pediatric population. The National Trauma Databank (NTDB) was queried from 2007 to 2015. Test and training datasets of the total number of patients (885,100) with complete ICD data were used to build a random forest model predicting BCVI. All ICD features not used to define BCVI (2268) were included within the random forest model, a machine learning method. A random forest model of 1000 decision trees trying 7 variables at each node was applied to training data (50% of the dataset, 442,600 patients) and validated with test data in the remaining 50% of the dataset. In addition, Denver and Memphis model variables were re-validated and compared to our new model. A total of 885,100 pediatric patients were identified in the NTDB to have experienced blunt pediatric trauma, with 1,998 (0.2%) having a diagnosis of BCVI. Skull fractures (OR 1.004, 95% CI 1.003–1.004), extremity fractures (OR 1.001, 95% 1.0006–1.002), and vertebral injuries (OR 1.004, 95% CI 1.003–1.004) were associated with increased risk for BCVI. The BCVI prediction model identified 94.4% of BCVI patients and 76.1% of non-BCVI patients within the NTDB. This study identified ICD9/ICD10 codes with strong association to BCVI. The Denver and Memphis criteria were re-applied to NTDB data to compare validity and only correctly identified 13.4% of total BCVI patients and 99.1% of non BCVI patients. The prediction model developed in this study is able to better identify pediatric patients who should be screened with further imaging to identify BCVI. Retrospective diagnostic study–level III evidence. [ABSTRACT FROM AUTHOR]

This study described the results of online interprofessional education (IPE) between physical therapy and health information management students. Using the published Student Perceptions of Interprofessional Clinical Education -- Revised, version 2 (SPICE-R2) survey, this study measured changes in perception about IPE before and after three online interactions. Survey results included an overall score and three factors: Interprofessional Teamwork and Team-Based Practice (T), Roles/Responsibilities for Collaborative Practice (R), and Patient Outcomes from Collaborative Practice (O). Data were analyzed using two-way analysis of variance tests using time and program as factors. The overall scores improved significantly for time (ρ=.019). The T factor demonstrated a significant change for program (ρ=.006) and the R factor improved significantly over time (ρ=.005) and by program (ρ=.022). Narrative student comments focused on role and responsibility clarification, communication and coordination, and participation in a realistic experience involving multiple professions. The students believed that the experience was beneficial and important. [ABSTRACT FROM AUTHOR]

In this paper we examine the failure of the zero carbon homes agenda in the UK and argue that it represents a case of policy dismantling, where a range of policies and programmes have been introduced, revised and then removed by government. We bring together the sustainability transitions literature with the literature on policy dismantling, regime resistance and regime detractors, and suggest that the zero carbon housing agenda in the UK offers useful insights into the politics of sustainability transitions. We identify three phases of policy change, from policy expansion, symbolic dismantling and eventual active policy dismantling. In the conclusions we offer some suggestions on processes of policy dismantling and what this might mean for future sustainability transitions given the significant contributions of buildings to greenhouse gas emissions and the urgent need for rapid low carbon transitions. [ABSTRACT FROM AUTHOR]

The development of new biomaterials and drug delivery systems necessitates animal experimentation to demonstrate biocompatibility and therapeutic efficacy. Reduction and replacement of the requirement to conduct experiment using full-grown animals has been achieved through utilising zebrafish embryos, a promising bridge model between in vitro and in vivo research. In this review, we consider how zebrafish embryos have been utilised to test both the biocompatibility of materials developed to interact with the human body and drug release studies. Furthermore, we outline the advantages and limitations of this model and review legal and ethical issues. We anticipate increasing application of the zebrafish model for biomaterial evaluation in the near future. This review aims to evaluate the potential application and suitability of the zebrafish model in the development of biomaterials and drug delivery systems. It creates scientific impact and interest because replacement models are desirable to the society and the scientific community. The continuous development of biomaterials calls for the need to provide solutions for biological testing. This review covers the topic of how the FET model can be applied to evaluate biocompatibility. Further, it explores the zebrafish from the wild-type to the mutant form, followed by a discussion about the ethical considerations and concerns when using the FET model. Image, graphical abstract [ABSTRACT FROM AUTHOR]

While nearly all hospitals have adopted electronic health record (EHR) systems, some are dissatisfied and considering replacement systems to better address unique organizational needs and priorities. With more than 4,000 certified health information technology products available, comparing the vast number of EHR options is complex. This study tested the hypothesis that various EHR systems demonstrate different financial and quality performance and presented a framework for comparison. Using a subscribed database containing US hospitals' observations from 2011 to 2016, we estimated an ordinary least squares regression model with robust standard errors and clustered by year. We regressed the selected finance and quality measures as dependent variables with the vendors' indicators as independent variables, with control variables. This study demonstrated an approach for analyzing performance data to help hospitals distinguish EHR systems on the basis of several organizational outcomes: return on assets, bed utilization rate, Hospital Consumer Assessment of Healthcare Providers and Systems (HCAHPS) summary star rating, and value-based purchasing Total Performance Score. This framework will help EHR acquisition teams make informed decisions. [ABSTRACT FROM AUTHOR]

• Programs can be distinguished by purpose, audience, methods, approach to uncertainty and assumptions, and more. • Programs' timeframes varied from <2 to 100 years, with many focused on the 20–40 year range. • Environmental futures programs are strengthened by using multiple methods and defining success in advance. • Seven practices can promote the successful uptake of environmental futures information by decision-makers. Environmental decision- and policy-making must contend with uncertainty about the future that can hinder proactive environmental decisions, forcing environmental managers into reactive postures. To help prevent this, a variety of methods exist for exploring potential environmental futures and associated uncertainties. Producing environmental futures benefits those involved by supporting a systems-level understanding and expanding thinking beyond "business as usual". We review select environmental and non-environmental futures programs that seek to inform decision-making. Review objectives are to: 1) identify and discuss key environmental futures program attributes, and 2) propose program attributes that support successful connection with decision processes. Attributes discussed include purpose and audience, methods, addressing uncertainty and assumptions, relationship to indicator programs, how program values and biases are addressed, the role of goals, and how success is measured and defined. We conclude with recommendations for how to conduct environmental futures programs to be more useful for environmental decisions. These include: combining multiple environmental futures methods to provide complementary insights or highlight inconsistencies in assumptions, including a schematic of assumptions and drivers, and defining success criteria, whenever possible. These practices can help increase the acceptance of environmental futures products in decision-making and increase their short-term and long-term contributions. [ABSTRACT FROM AUTHOR]

Climate change is predicted to contribute to the present wave of extinctions; however, these extinctions may not immediately follow temperature increases and other climate change effects. We examined how predator species composition and increased temperatures affected bacterivorous protist community extinction debt (the number of species that will go extinct) and relaxation time (the amount of time it takes for the extinction debt to be paid off). We found that neither affected relaxation time, but that the presence of a generalist predatory protist species increased extinction debt through its role as a keystone predator. Warming also significantly decreased how long it took communities to lose half of the species that were to eventually go extinct. That this half-relaxation time was shorter in warmed communities than in unwarmed communities in this system indicates that extinctions were front-loaded in the warmed communities compared to the unwarmed communities. Our findings suggest that even if increased temperature is not expected to increase extinction debt, it could still accelerate the rate at which those extinctions will occur. This in turn narrows the time window for taking actions to prevent a large portion of the extinction debt from being paid off. [ABSTRACT FROM AUTHOR]

Highly alkaline industrial residues (e.g., steel slag, bauxite processing residue (red mud) and ash from coal combustion) have been identified as stocks of potentially valuable metals. Technological change has created demand for metals, such as vanadium and certain rare earth elements, in electronics associated with renewable energy generation and storage. Current raw material and circular economy policy initiatives in the EU and industrial ecology research all promote resource recovery from residues, with research so far primarily from an environmental science perspective. This paper begins to address the deficit of research into the governance of resource recovery from a novel situation where re-use involves extraction of a component from a bulk residue that itself represents a risk to the environment. Taking a political industrial ecology approach, we briefly present emerging techniques for recovery and consider their regulatory implications in the light of potential environmental impacts. The paper draws on EU and UK regulatory framework for these residues along with semi-structured interviews with industry and regulatory bodies. A complex picture emerges of entwined ownerships and responsibilities for residues, with past practice and policy having a lasting impact on current possibilities for resource recovery. [ABSTRACT FROM AUTHOR]

Experiential learning via simulation offers a variety of benefits including reduced risks, repetitive exposure, and mastery of complex processes. How to motivate people to engage in and enjoy playing games is an important concept in the creation of serious games focused on learning new skills. This study sought to determine the motivators that increase users' pleasurable experience when playing an electronic health record simulation game. To examine how intrinsic and extrinsic motivation affected both engagement and enjoyment, we surveyed students of health professions at one university. Results indicate that while both forms of motivation are significant in increasing engagement and enjoyment, extrinsic motivation such as badges, points, and scoreboards were much more important than internal motivations for our participants. These findings have implications for the development of an electronic health record simulation game. [ABSTRACT FROM AUTHOR]

The article focuses on the self-interest of the non-executive director with measuring the elements of corporate governance and the corporate scandals and an increased ability for companies to diversify and it identify and analyse matters such as the nature of the conflict.

The article focuses on advantages and disadvantages of print books and eBooks and mentions survey from Library Journal that confirmed college students preferred to read eBooks for research and read print books for pleasure. Topics discussed include eBook experience offered by public libraries to patrons in comparision to academic libraries, online access provided by subscribing libraries as P (Print) + E (Electronic_ and lack of availability of monographs through the model.

The article discusses research on inherited genetic variants such as TYRP1 (Tyrosinase-related protein 1 gene) associated with multiple primary melanoma. Topics discussed include determining genetic predisposition for multiple primary melanoma in the Genes, Environment, and Melanoma Study. Topics discussed include assessment of effects of single nucleotide proteins, evidences for relevance of genetic regions to melanoma risk, and magnitude of genetic effect on development of primary melanoma.