1. Floating cells with stem cell properties in gastric cell line SGC-7901
- Author
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Zheng, Qiuhong, Gong, Fusheng, Xu, Yangmei, Zheng, Tianrong, and Ying, Mingang
- Abstract
Aims To obtain floating spheres from the adherent gastric cancer cell line SGC-7901 and to analyze the properties of the spheres.Methods Serum-free medium was applied to cultured SGC-7901 cells. Limiting dilution assay, tumor formation assay, microarray analysis, and real-time fluorescent quantitative PCR were used to test the stem cell properties of the spheres.Results A subpopulation of SGC-7901 cells formed floating spheres in serum-free medium. These cells showed enhanced tumorigenic ability and also highly expressed certain stem-cell-associated proteins.Conclusions We successfully propagated floating spheres with stem cell properties in the SGC-7901 gastric cell line.
- Published
- 2011
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