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In this study, in order to assess thermal runaway risk of two step synthesis reactions, two new risk assessment methods were proposed by combining the new assessment procedure with the risk matrix and the Stoessel criticality diagram. The thermal runaway risks of the tert-butyl peroxybenzoate (TBPB) synthesis process and the tert-butyl peracetate (TBPA) synthesis process, and the influence of the use of the pure target products on the assessment results were also studied by the new methods. The results showed that the new methods could obtain more accurate assessment results of two step synthesis reactions. Some measures should be taken to reduce the thermal runaway risks of the two synthesis processes. Furthermore, the use of the pure target products in the new methods would exaggerate the thermal runaway risks of the two step synthesis reactions.

Recent studies have demonstrated that miR-202 is associated with several types of cancer; however, the expression and function of miR-202 have not been investigated in bladder cancer. We analyzed the expression of miR-202 in bladder cancer tissues and adjacent noncancerous tissues. The effect of miR-202 on the proliferation, migration, and invasion was evaluated by in vitro assays. The target gene of miR-202 was assessed by luciferase reporter assay. In this study, miR-202 was found to be significantly downregulated in bladder cancer cell lines and tissues and was highly correlated with the T classification, N classification, grade, and recurrence. Ectopic expression of miR-202 suppressed cell viability, colony formation, cell migration, and invasion in vitro and inhibited xenograft tumor growth in vivo. Inversely, downregulation of miR-202 had contradictory effects. The 3′-untranslated region (3′-UTR) of epidermal growth factor receptor (EGFR) was identified as a direct target of miR-202 using luciferase reporter assays, and knockdown of EGFR enhanced miR-202-inhibited cell proliferation, migration, and invasion. In conclusion, miR-202 suppresses bladder cancer carcinogenesis and progression by targeting EGFR, thereby representing a potential target for miRNA-based therapy for bladder cancer in the future.