7 results on '"Hanyu, K."'
Search Results
2. Tudor protein is essential for the localization of mitochondrial RNAs in polar granules of Drosophila embryos
- Author
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Amikura, R., Hanyu, K., Kashikawa, M., and Kobayashi, S.
- Published
- 2001
- Full Text
- View/download PDF
3. Ca(2+)/calmodulin and p85 cooperatively regulate an initiation of cytokinesis in Tetrahymena.
- Author
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Gonda, K, Katoh, M, Hanyu, K, Watanabe, Y, and Numata, O
- Abstract
Tetrahymena p85 differs in mobility in two-dimensional SDS-polyacrylamide gel electrophoresis between wild-type and temperature-sensitive cell-division-arrest mutant cdaA1 cell extracts, and is localized to the presumptive division plane before the formation of the division furrow. The p85 contained three identical sequences which show homology to the calmodulin binding site of Ca(2+)/calmodulin dependent protein kinase Type II in Saccharomyces cerevisiae. We found the p85 directly interacts with Tetrahymena calmodulin in a Ca(2+)-dependent manner, using a co-sedimentation assay. We next examined the localization of p85 and calmodulin during cytokinesis using indirect immunofluorescence. The results showed that both proteins colocalize in the division furrow. This is the first observation that calmodulin is localized in the division furrow. Moreover, the direct interaction between p85 and Ca(2+)/calmodulin was inhibited by Ca(2+)/calmodulin inhibitor N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide HCl. When the cells were treated with the drug just before the beginning of cytokinesis, the drug also inhibited the localization of p85 and calmodulin to the division plane, and the formation of the contractile ring and division furrow. Therefore, we propose that the Ca(2+)/calmodulin signal and its target protein p85 cooperatively regulate an initiation of cytokinesis and may be also concerned with the progression of cytokinesis in Tetrahymena.
- Published
- 1999
4. Identification of a mammary transforming gene (MAT1) associated with mouse mammary carcinogenesis.
- Author
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Bera, T K, Guzman, R C, Miyamoto, S, Panda, D K, Sasaki, M, Hanyu, K, Enami, J, and Nandi, S
- Abstract
We have developed an efficient in vitro transformation system using N-methyl-N-nitrosourea that allows us to study the role of hormones and growth factors in mouse mammary tumorigenesis. Utilizing this system, we reported earlier that mammary tumors induced in vitro with N-methyl-N-nitrosourea in the presence of mammogenic hormones (progesterone and prolactin) contain predominately an activated c-Ki-ras protooncogene with a G35 --> A35 transitional mutation in the 12th codon. Mammary tumors induced in the presence of another mitogen, lithium (Li), do not have a mutation in the c-Ki-ras protooncogene. By using an expression cloning system, a plasmid clone containing a 1.75-kb cDNA insert has been isolated from this group of tumors. Nucleic acid sequence analysis of the insert reveals that it has a short open reading frame of 61 amino acids and that it does not have sequence homology with any known gene. The gene, designated MAT1, can neoplastically transform NIH 3T3 cells and also the mammary epithelial cell line TM3. Expression of this gene occurs in normal mouse tissues including mammary gland and is overexpressed in the original mammary tumors as indicated by Northern blot analysis. In vitro transcription and translation of the clone shows a protein product of 6000 Da, which agrees with the predicted open reading frame.
- Published
- 1994
- Full Text
- View/download PDF
5. Linkage between recycling and consumption: a case of toilet paper in Japan
- Author
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Yamashita, H., Kishino, H., Hanyu, K., and Hayashi, C.
- Subjects
WASTE management ,TOILET paper ,CONSERVATION of natural resources ,WASTE recycling - Abstract
This study examines consumer factors of paper recycling in Japan. The study specifically focuses on toilet paper as a paper product and attempts to reveal how individuals evaluate recycled toilet paper, howthe evaluation relates to toilet paper consumption, and why people use or do not use recycled toilet paper. The study also examines what factors influence collection recycling behavior, and what people believe as necessary to achieve a society with better recycling. Responses were obtained from 1242 respondents in Japan. Four results were found. (1) People cannot judge the raw material of virgin toilet papers correctly, while people can correctly judge the raw material of recycled toilet paper. The quality and appearance of recycled toilet paperwas not high enough to compete with virgin toilet paper. Furthermore, the image of recycled toilet paper also had negative impact on the willingness to use recycled toilet paper. (2) The primary criterion for purchasing recycled toilet paper was pro-environmental attitude. For the virgin toilet paper, it was brand. As expected, recycled toilet paper users had a positive evaluation and image of recycled toilet paper, while virgin toilet paper users had a negative evaluation and image of it. (3) Actual recycling behavior might not relate directly to consumption behavior of recycled paper. Rather, it was determined by the knowledge of waste collection system and payment system. (4) Most people have not realized that without the consumption of recycledproducts, the recycling system is not completed. [ABSTRACT FROM AUTHOR]
- Published
- 2000
6. Circulation indices: new tools for analyzing the structure of material cascades
- Author
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Hayashi, C., Yamashita, H., Kishino, H., Hanyu, K., and Abe, K.
- Subjects
MATHEMATICAL models ,WASTE recycling ,PAPER recycling - Abstract
An improved understanding of the cascading structure of recycling isimportant for increasing resource productivity. The cascading theory, developed to analyze resource cascading, has two major dimensions; resource quality and resource lifetime. The latter is the only dimension for which a quantitative evaluation has been carried out in the previous research. In this study, we propose new tools to quantify both dimensions. These tools enable statistical estimation of the times of utilization of the material cascaded. The pre-circulation index (pre-CI) counts how many times the material has been utilized before consumption. For material in which quality deterioration is measurable largely on the basis of the number of utilization times, pre-CI can be an index of resource quality. The post-circulation index (post-CI) counts how many times the material will be utilized after consumption. The higher the number of utilization times, the longer the lifetimeof the material. Thus, post-CI can be an index of resource lifetime.Total-circulation index (TCI) is the sum of pre- and post-CIs. This can be an overall index of resource productivity. We apply the methodpresented herein to an analysis of the paper recycling system in Japan. Some important features of the CIs are demonstrated by the results; certain potential remains for further improvements in paper recycling in Japan. In the case that two products have the same utilizationrates, their pre-CIs can still differ according to the positions they occupy in the cascade. In the case that two recycling policies achieve the same size of primary raw material reduction, they can still differ in their impact to the whole cascade. By the method described herein, the CIs can represent the structure of a material cascade quantitatively and offer important knowledge by which to increase resource productivity. [ABSTRACT FROM AUTHOR]
- Published
- 2000
7. Recycling and consumption in Germany and Japan: a case of toilet paper
- Author
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Yamashita, M., Hayashi, C., Kishino, H., and Hanyu, K.
- Subjects
WASTE recycling ,TOILET paper - Abstract
This paper compares the recycling attitudes of consumers in Germany and Japan, based on our nationwide questionnaires. We analyzed consumption behavior as well as recycling behavior in narrow sense, becauseit is important to study the former to see how the supply of recycled materials is balanced with the demand, particularly in Japan which exports little recycled materials. Toilet paper was adopted as a specific good for study, because consumers have options of buying virgin products and recycled products. It was found that German households pay for waste collection specifically and have higher recycling rates than Japanese households. On the other hand, similar figures in German and Japanese consumers were observed with regard to consumption of recycled products. Their purchasing criteria and preference on virginand recycled products of toilet paper were examined by self-report and blind test with 2x 2 experimental design for toilet paper (German versus Japanese, virgin versus recycled). Blind test showed that people prefer domestic and virgin products in both countries. Half of respondents rating virgin products guessed that the products contained recycled material. For Japanese, the material of the sample toilet paper, made from virgin pulp, seems to be a more determining factor thanthe nationality of products, i.e. made in Japan. On the other hand, for Germans, the domestic nature seems to be a more dominant factor than the material, i.e. made from virgin material. Canonical discriminant analysis in conjunction with logistic regression based on self-reporting data detected the characters `multiplied', `appearance' and `brand' as major factors making the difference in preference between the two countries. The possible reasons causing the similarity and dissimilarity between the two countries are discussed with reference to the background history. [ABSTRACT FROM AUTHOR]
- Published
- 1999
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